A rat mammary gland cancer cell with stem cell properties of self-renewal and multi-lineage differentiation.

abstract：:Advancements in cell cultures are occurring at a rapid pace, an important direction is culturing cells in 3D conditions. We demonstrate the usefulness of agarose hydrogels in obtaining 3 dimensional aggregates of three cell lines, A549, MCF-7 and Sp2/0. The differences in culture phases, susceptibility to cisplatin-in...

journal_title：Cytotechnology

authors： Ravi M,Kaviya SR,Paramesh V

更新日期：2016-05-01 00:00:00

abstract：:A new compound, rotenoid isoflavone glycoside named, 6'-O-beta-D: -glucopyranosyl-12a-hydroxydalpanol was isolated from the methanolic (MeOH) fruit extract of Amorpha fruticosa LINNE by means of multi-stage column chromatography. Immuno-modulatory activities of this new glycoside were compared with the partitioned fra...

journal_title：Cytotechnology

authors： Lee HJ,Kang HY,Kim CH,Kim HS,Kwon MC,Kim SM,Shin IS,Lee HY

更新日期：2006-11-01 00:00:00

abstract：:To determine the function of miR-206 in epilepsy. Epileptic rat model was established by intra-amygdala injection of kainic acid (KA). Expression levels of miR-206, C-C Motif Chemokine Ligand 2 (CCL2) and interleukin-1β (Il-1β) in hippocampus tissues was measured by reverse transcription-quantitative PCR (RT-qPCR) and...

journal_title：Cytotechnology

authors： Wu Z,Liu Y,Huang J,Huang Y,Fan L

更新日期：2019-06-26 00:00:00

abstract：:Endothelial cells are involved in important pathological situations. They could be the target for infectious processes as for example in Cowdriosis, an important disease in cattle due to the rickettsia Cowdria ruminantium prevalent in the south of the Sahara. They are also connected to angiogenic processes related to ...

journal_title：Cytotechnology

authors： Marique T,Blankaert D,Hendrick V,Raschella A,Declerck B,Alloin C,Teixera-Guerra I,Sandron D,Cherlet M,Parent D,Kirkpatrick C,Van Vooren JP,Wérenne J

更新日期：1997-11-01 00:00:00

abstract：:In vitro studies about biomaterials biological properties are essential screening tests. Yet cell cultures encounter difficulties related to cell retention on material surface or to the observation of both faces of permeable materials. The objective of the present study was to develop a reliable in vitro method to stu...

journal_title：Cytotechnology

authors： Grenade C,Moniotte N,Rompen E,Vanheusden A,Mainjot A,De Pauw-Gillet MC

更新日期：2016-12-01 00:00:00

abstract：:Iron overload is a major health problem for patients who have to have continuous blood transfusions. It brings some metabolic problems together. Various iron chelating agents are being used for treatment of hemochromatosis which arises from excess iron accumulation. This study was conducted with the aim of determining...

journal_title：Cytotechnology

authors： Ila HB,Topaktas M,Arslan M,Büyükleyla M

更新日期：2014-08-01 00:00:00

abstract：:Taking into account that oxidative stress is among the factors causing cancer-related death; chemoprevention which consists in using antioxidant substances such as phenolics could prevent cancer formation and progression. In the present study, phenolic contents and antioxidant activities of methanolic extracts from th...

journal_title：Cytotechnology

authors： Boulaaba M,Tsolmon S,Ksouri R,Han J,Kawada K,Smaoui A,Abdelly C,Isoda H

更新日期：2013-12-01 00:00:00

abstract：:"In vitro" studies, commonly using porcine ovarian follicles, may generate inconsistent results when atretic follicles are not eliminated from the pool of experimental follicles. The present experiment was conducted to test the practical value of the macroscopie identification of large porcine follicles, which were as...

journal_title：Cytotechnology

authors： Dudek D

更新日期：1994-01-01 00:00:00

abstract：:The Korean horse mussel extract was purified and fractionated by a bioassay-guided purification step. The final fraction contained seven steroid and one polycyclic aromatic compounds, in which cholest-7-en-3-ol, (3β,5α)- (58.7 %) was a main component followed by ergosta-7,22dien-3-ol (3β,5α,22E) (13.0 %). This extract...

journal_title：Cytotechnology

authors： Wikarta JM,Kim SM

更新日期：2016-08-01 00:00:00

abstract：:In our previous work, we isolated Arbas Cashmere goat hair follicle stem cells (gHFSCs) and explored the pluripotency. In this study, we investigated the expression and putative role of Sox9 in the gHFSCs. Immunofluorescence staining showed that Sox9 is predominantly expressed in the bulge region of the Arbas Cashmere...

journal_title：Cytotechnology

authors： He N,Dong Z,Tai D,Liang H,Guo X,Cang M,Liu D

更新日期：2018-08-01 00:00:00

abstract：:Despite the fact, that monoclonal antibodies are the fastest growing group of biopharmaceuticals in development, this is not true for the IgM class, which remains as enigmatic as ever. While more examples of usefulness of IgMs for medical applications are emerging, their recombinant production is still not common. In ...

journal_title：Cytotechnology

authors： Chromikova V,Mader A,Steinfellner W,Kunert R

更新日期：2015-03-01 00:00:00

abstract：:The bioavailability and action of teas on the invasion of a rat ascites hepatoma cell line, AH109A, were determined and their modes of action were by co-culturing the cancer cells with a rat mesentery-derived mesothelial-cell (M-cell) monolayer in the presence of sera from rats orally given teas and their component, (...

journal_title：Cytotechnology

authors： Zhang G,Miura Y,Yagasaki K

更新日期：2001-07-01 00:00:00

abstract：:The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rb1 was produced by fusing splenocytes immunized with a ginsenoside Rb1- bovine serum albumin conjugate wit...

journal_title：Cytotechnology

authors： Tanaka H,Fukuda N,Shoyama Y

更新日期：1999-03-01 00:00:00

abstract：:We describe possible functions of carbohydrates attached to growth factors and strategies to examine the functions, concentrating on erythropoietin, a major regulator of erythropoiesis. Erythropoietin in erythropoiesis functions as an endocrine hormone; it is produced by kidney cells and transferred into the circulati...

journal_title：Cytotechnology

authors： Nagao M,Masuda S,Ueda M,Sasaki R

更新日期：1995-01-01 00:00:00

abstract：:The bone marrow represents the most common source from which to isolate mesenchymal stem cells (MSCs). MSCs are capable of differentiating into tissues of the three primary lineages and have the potential to enhance repair in damaged organs through the principals of regenerative medicine. Given the ease with which MSC...

journal_title：Cytotechnology

authors： Dickinson H,Milton P,Jenkin G

更新日期：2012-10-01 00:00:00

abstract：:The presence of a highly purified recombinant interleukin-2 (rIL-2) increased the production of immunoglobulin (IgM or IgG) by human-human hybridomas to 1.5-2.0 times the production by untreated cells. However, these cells did not react with anti-Tac (IL-2 receptor alpha) antibody. To enhance the response of the hybri...

journal_title：Cytotechnology

authors： Iwamoto K,Shintani Y,Sasada R,Honjo T,Kitano K

更新日期：1990-07-01 00:00:00

abstract：:We screened for biological activity which induces neurite outgrowth in vitro from 300 species of marine algae from along the Japan coast for possible use as a treatment for the lack of neurotrophic factor which is considered to be a cause of Alzheimer's disease. In this study, we evaluated the neurite outgrowth promot...

journal_title：Cytotechnology

authors： Kamei Y,Sagara A

更新日期：2002-11-01 00:00:00

abstract：:Chemical genomics is a new research paradigm with importantapplications in drug discovery. It links genomic targets withsmall-molecule chemistries thereby allowing for efficient targetvalidation and lead compound identification. ACADIA'schemical-genomics platform consists of a large and diverse small-moleculelibrary (...

journal_title：Cytotechnology

authors： Hacksell U,Nash N,Burstein ES,Piu F,Croston G,Brann MR

更新日期：2002-01-01 00:00:00

abstract：:The growth of rat adrenal nerve cells was remarkably enhanced by supplementing the cultured medium from the human fibroblast cell line, Hs 68. Maximum specific growth rate and length of the neurites were observed as 0.076 (1/hr) and 0.026 mm, respectively in 20% supplement of five day old medium. In adding more than 2...

journal_title：Cytotechnology

authors： Hong JS,Kim DS,Kim SH,Choi DH,Lee JH,Lee HY

更新日期：1998-03-01 00:00:00

abstract：:Primary rat hepatocytes dedifferentiate rapidly losing theactivities of the drug metabolising enzymes involved in thedetoxification of xenobiotics in the liver. An alternativeapproach to using primary hepatocytes for toxicity testing isthe development of immortalised hepatocyte cell lines via thetransfection of primar...

journal_title：Cytotechnology

authors： Kelly HT,Hill E,Reid F,Grant MH,Macdonald C

更新日期：2000-10-01 00:00:00

abstract：:In construction of epithelial cells as multilayers, the cells are grown submerged to confluence on fibroblast-embedded collagen gels and, then, lifted to air to promote their stratification. We recently demonstrated that gingival epithelial cells form uniform monolayers on semi-permeable nitrocellulose membranes, supp...

journal_title：Cytotechnology

authors： Gursoy UK,Gursoy M,Könönen E,Sintim HO,Uitto VJ,Syrjänen S

更新日期：2016-12-01 00:00:00

abstract：:The capacity of hepatocytes and cholangiocytes to contribute to their own maintenance has long been recognized. More recently, studies have indicated the presence of both intra-hepatic and extra-hepatic stem/progenitor cell populations. The intraorgan compartment probably derives primarily from the biliary tree, most ...

journal_title：Cytotechnology

authors： Theise ND

更新日期：2003-03-01 00:00:00

abstract：:The Atlantic salmon (Salmo salar) serum lectin (SSL) is a soluble C-type lectin that binds bacteria, including salmon pathogens. This lectin is a cysteine-rich oligomeric protein. Consequently, a Drosophila melanogaster expression system was evaluated for use in expressing SSL. A cDNA encoding SSL was cloned into a ve...

journal_title：Cytotechnology

authors： Uribe E,Venkatesan M,Rose DR,Ewart KV

更新日期：2013-08-01 00:00:00

abstract：:Aim We studied the role of mini-TyrRS and mini-TrpRS in angiogenesis by using small interfering RNA-mediated mini-TyrRS/mini-TrpRS knockout in hypoxic culture of human umbilical vein endothelial cells. Methods SiRNA was used as the main method to inhibited the gene function. Silencing efficiency was assayed by real-ti...

journal_title：Cytotechnology

authors： Zeng R,Chen YC,Zeng Z,Liu R,Qiang O,Jiang XF,Liu XX,Li X,Wang HY

更新日期：2008-03-01 00:00:00

abstract：:Medium-chain fatty acids (MCFAs) have been suggested as an alternative to the use of antibiotics in animal nutrition with promising results. First, we studied the sensitivity of Salmonella Enteritidis and an enteropathogenic Escherichia coli strain against caprylic (C8), capric (C10) and lauric (C12) acids. A porcine ...

journal_title：Cytotechnology

authors： Martínez-Vallespín B,Vahjen W,Zentek J

更新日期：2016-10-01 00:00:00

abstract：:The Baculovirus Expression Vector System has become widely used for the production of recombinant proteins for research and diagnostics. Serum-free culture media able to support high cell densities have been developed for the large scale culture of insect cells. While serum elimination aims at avoiding the risks assoc...

journal_title：Cytotechnology

authors： Deparis V,Durrieu C,Schweizer M,Marc I,Goergen JL,Chevalot I,Marc A

更新日期：2003-07-01 00:00:00

abstract：:Human herpes virus 6 (HHV-6) is a common human pathogen that is most often detected in hematopoietic cells. Although human cells harboring chromosomally integrated HHV-6 can be generated in vitro, the availability of such cell lines originating from in vivo tissues is limited. In this study, chromosomally integrated H...

journal_title：Cytotechnology

authors： Shioda S,Kasai F,Ozawa M,Hirayama N,Satoh M,Kameoka Y,Watanabe K,Shimizu N,Tang H,Mori Y,Kohara A

更新日期：2018-02-01 00:00:00

abstract：:Megalocytiviruses are important emerging pathogens in both freshwater and marine finfish aquaculture. However, a limited number of piscine cell lines are persistently susceptible to these viruses, which greatly limits the study of megalocytiviruses. In this study, a new fibroblast-like cell line was established from a...

journal_title：Cytotechnology

authors： Dong C,Shuang F,Weng S,He J

更新日期：2014-12-01 00:00:00

abstract：:Sorbaria kirilowii (Regel) Maxim, a plant found in China, Korea, Japan, and east of Europe, is a common herb used for traditional medicinal purposes. However, its ability to prevent photoaging has not been studied. In this study, we investigated the anti-photoaging functions of an ethanol extract (Sk-EE) of S. kirilow...

journal_title：Cytotechnology

authors： Chen H,Jang J,Kopalli SR,Yum J,Yoon K,Cho JY

更新日期：2021-02-01 00:00:00

abstract：:The establishment of a cell culture system promoting chondrocyte differentiation has been utilized to better characterize phenotypic stages of chondrogenesis at the cellular level. Although the expression of the type II collagen gene has been studied during "in vitro" chondrocyte differentiation, little is known about...

journal_title：Cytotechnology

authors： Castagnola P,Cancedda R

更新日期：1991-02-01 00:00:00