Abstract:
:In neuronal dendrites, septins localize to the base of the spine, a unique position which is sandwiched between the microtubule (MT)-rich dendritic shaft and the actin filament-rich spine. Here, we provide evidence for the association of SEPT6 with MTs in cultured rat hippocampal neurons. In normal cultures, SEPT6 clusters localized to MTs, but not to actin clusters. Only MT-disrupting agents (vincristine and nocodazole), but not microfilament-disrupting one (latrunculin A), induced the redistribution of SEPT6 to the disrupted MTs. The nascent MT fibers that were recovered from vincristine or nocodazole treatments also accompanied SEPT6. Blocking MT disruption by Taxol prevented such phenomena, proving that the redistribution of SEPT6 was due to the MT disruption. Our results indicate that SEPT6 complexes at the base of the dendritic spine are associated with MTs.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Moon IS,Lee H,Walikonis RSdoi
10.1007/s10616-012-9477-7subject
Has Abstractpub_date
2013-03-01 00:00:00pages
179-86issue
2eissn
0920-9069issn
1573-0778journal_volume
65pub_type
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