Abstract:
:The usefulness of host range expanded viruses as an expressionvector system was investigated by following the expression ofthe E. coli lacZ gene. The host range expanded recombinantviruses were obtained from Sf-21 or BmN-4 cells coinfected withAutographa californica and Bombyx mori nuclearpolyhedrosis viruses. Among the host range expanded viruses,RecB-8 and RecS-B6 have similar enzyme digestion profiles butdifferent infection characteristics in cells. Therefore, tostudy the foreign gene expression efficiency of these twoviruses, we constructed recombinant viruses RecB8-LacZ andRecSB6-LacZ containing the lacZ gene instead of the polyhedringene. Also, the host range expanded recombinant AcNPV, Bac-BH,containing lacZ gene in the polyhedrin gene locus was constructedby substitution of the 0.6 kb region within the helicase gene ofBacPAK6 with that of BmNPV. beta-Galactosidase expressionefficiency by these viruses were determined and compared in Sf-21and BmN-4 cells. The result showed that Bac-BH has highexpression efficiency only in Sf-21 cells, whereas RecB8-LacZhas high expression efficiency both in Sf-21 and BmN-4 cells.Also, in BmN-4 cells, beta-galactosidase expressionefficiency of RecB8-LacZ was higher than that of recombinantBmNPV (BmK1-LacZ containing lacZ gene in polyhedrin gene locus).In addition, the expression efficiency was not correlated withvirus titer.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Kim HS,Woo SD,Kim WJ,Choi JY,Kang SKdoi
10.1023/A:1008166310368subject
Has Abstractpub_date
2000-02-01 00:00:00pages
87-92issue
2eissn
0920-9069issn
1573-0778journal_volume
32pub_type
杂志文章相关文献
CYTOTECHNOLOGY文献大全abstract::The development of specific catalytic inhibitors for the serine protease urokinase-type plasminogen activator (uPA) has been hindered due to difficulties in producing sufficient amounts of active recombinant uPA that is catalytically equivalent to native uPA. The purpose of this study was to develop an efficient syste...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-005-4637-7
更新日期:2005-09-01 00:00:00
abstract::The effects of media concentrations of glucose andglutamine on the intracellular nucleotide pools andoxygen uptake rates of a murine antibody-secretinghybridoma cell line were investigated. Cells takenfrom mid-exponential phase of growth were incubated inmedium containing varying concentrations of glucose(0-25 mM) and...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008154615643
更新日期:2000-10-01 00:00:00
abstract::The establishment of a cell culture system promoting chondrocyte differentiation has been utilized to better characterize phenotypic stages of chondrogenesis at the cellular level. Although the expression of the type II collagen gene has been studied during "in vitro" chondrocyte differentiation, little is known about...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00736804
更新日期:1991-02-01 00:00:00
abstract::Rats subcutaneously implanted with AH109A hepatoma cells show hyperlipidemia with high concentrations of serum triglyceride and nonesterified fatty acid, suppression of lipoprotein lipase (LPL), and elevation of hormone-sensitive lipase (HSL) activities during the growth of the hepatoma. Supplementation of the diet wi...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-010-9282-0
更新日期:2010-07-01 00:00:00
abstract::Chicken egg yolk lipoprotein (YLP) was partially fractionated into some constituents, and the effect of constituents of YLP were examined on the growth and immunoglobulin (IgM and IgG) secretion of a HB4C5 human-human hybridoma cell line cultured in serum-free medium. Among the fractions, YP-1 and YP-2 fractions (LDL-...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00749004
更新日期:1993-01-01 00:00:00
abstract::The current study was conducted to determine whether there is a relation between hypertension and two different polymorphisms, including C1562T of the Matrix metalloproteinase-9 (MMP-9) gene and C677T of the methylenetetrahydrofolate reductase (MTHFR) gene. Genomic DNA obtained from 224 persons (125 patients with hype...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-013-9665-0
更新日期:2015-01-01 00:00:00
abstract::Recent studies have indicated that Di-(2-ethylhexyl) phthalate (DEHP), the most commonly used plasticizer in daily-life products, could be dispersed in indoor air and induce human exposure via inhalation. DEHP has been reported to have effects on the respiratory system in both animal and human researches. The toxicity...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-017-0111-6
更新日期:2018-02-01 00:00:00
abstract::A fructose-based cell culture is suitable for the process control of protein production because of slow sugar consumption rate and low lactate accumulation. The fructose transporter, GLUT5, mediates its incorporation into cells and is required for the fructose-based culture. In order to produce efficiently recombinant...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-010-9289-6
更新日期:2010-08-01 00:00:00
abstract::The decline of European abalone Haliotis tuberculata populations has been associated with various pathogens including bacteria of the genus Vibrio. Following the summer mortality outbreaks reported in France between 1998 and 2000, Vibrio harveyi strains were isolated from moribund abalones, allowing in vivo and in vit...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-013-9583-1
更新日期:2013-10-01 00:00:00
abstract::In the recent years, the possibility of utilizing extracellular vesicles for drug delivery purposes has been investigated in various models, suggesting that these vesicles may have such potential. In addition to the choice of donor cell type for vesicle production, a major obstacle still exists with respect of loading...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-016-9952-7
更新日期:2016-10-01 00:00:00
abstract::By employing the affinity gel fraction technique, we have detected a 175 kDa tyrosine-O-sulfate (TyrS)-binding protein in sodium choleate extracts of the microsomal membrane fractions of bovine liver and pancreas, as well as canine liver and pancreas. Western blot analysis revealed the presence of the bovine liver Tyr...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1007903315687
更新日期:1997-01-01 00:00:00
abstract::Material and degree of reductance balance equations are used to estimate the rates of oxygen uptake and carbon dioxide evolution of animal cell cultures. Lumped compositions, molecular weight and reductance degree of cellular protein, monoclonal antibody, biomass and amino acid consumption (excluding glutamine and ala...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008004618163
更新日期:1999-05-01 00:00:00
abstract::Olive oil intake has been shown to induce significant levels of apoptosis in various cancer cells. These anti-cancer properties are thought to be mediated by phenolic compounds present in olive. These beneficial health effects of olive have been attributed, at least in part, to the presence of oleuropein and hydroxyty...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-009-9191-2
更新日期:2009-01-01 00:00:00
abstract::Early developments in serum-free media led to a variety of formulations in which components normally provided in serum and required for growth (insulin, transferrin, lipid supplements, trace elements) and poorly defined components (extracts, hydrolysates) were added to defined basal media. These additives were mostly ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-006-9002-y
更新日期:2006-03-01 00:00:00
abstract::Actions of chlorogenic acid, a major component of coffee, andits constituents, caffeic and quinic acids, on theproliferation and invasion of AH109A, a rat ascites hepatomacell line, were investigated using in vitro assay systems. Allthree components suppressed the AH109A invasion atconcentrations of 5-40 muM without a...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008141918852
更新日期:2000-07-01 00:00:00
abstract::In order to establish an efficient culture system for the generation of lymphokine activated killer (LAK) cells, we have developed a new device which is essentially based on a continuous dialyzing culture vessel. LAK cells grown in such a system showed higher cytotoxicity than those grown under conventional culture co...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00365414
更新日期:1989-02-01 00:00:00
abstract::Achyranthes bidentata (A. bidentata) Blume is a medicinal herb with the property of strengthening bones and muscles and ensuring proper downward flow of blood in terms of the therapeutic theory of traditional medicine. In the present study, the effect of A. bidentata root extract (AE) on osteoblast function was invest...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-013-9645-4
更新日期:2014-12-01 00:00:00
abstract::"In vitro" studies, commonly using porcine ovarian follicles, may generate inconsistent results when atretic follicles are not eliminated from the pool of experimental follicles. The present experiment was conducted to test the practical value of the macroscopie identification of large porcine follicles, which were as...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00758179
更新日期:1994-01-01 00:00:00
abstract::A promising method for diabetes treatment is the implantation of immunoisolated cells secreting insulin in response to glucose. Cell availability limits the application of this approach at a medically-relevant scale. We explore the use of transformed cells that can be grown to large homogeneous populations in developi...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00762410
更新日期:1994-01-01 00:00:00
abstract::Alphaviruses are positive stranded RNA viruses that replicate to extremely high titers. Sindbis and Semliki Forest viral vectors are widely used tools for high-level production of recombinant proteins. Recent studies have broadened their scope to vaccine production, gene therapy, and analysis of cell function. Here we...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1013192017048
更新日期:2001-05-01 00:00:00
abstract::Successful culturing of neurons from adult animals has been historically difficult for a relatively long time. In this study, we reported the development of a novel method for the isolation and the culture of major pelvic ganglion (MPG) neurons from adult rat. The cultured cells were identified by neuron morphology an...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-012-9515-5
更新日期:2013-08-01 00:00:00
abstract::Although the insect cell/baculovirus system is an important expression platform for recombinant protein production, our understanding of insect cell metabolism with respect to enhancing cell growth capability and productivity is still limited. Moreover, different host insect cell lines may have different growth charac...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-013-9637-4
更新日期:2014-10-01 00:00:00
abstract::Celecoxib is a selective cyclooxygenase-2 (COX-2) inhibitor with antitumor and antiangiogenic activities. To investigate the effects of celecoxib on nasopharyngeal carcinoma (NPC), HNE-1 cells were treated with celecoxib at various concentrations. MTT assay, migration assay and invasion assay were performed to observe...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-010-9296-7
更新日期:2010-10-01 00:00:00
abstract::Models of cell processes can be particularly useful in simulating, optimizing and controlling cell culture systems. Models reported in the literature are of various degrees of biological structure and mathematical complexity and describe cell growth, death, metabolism, and product formation, alone or in combination wi...
journal_title:Cytotechnology
pub_type: 杂志文章,评审
doi:10.1007/BF00749616
更新日期:1994-01-01 00:00:00
abstract::We provide evidence that the expression of the human MRP/GS-X pump encoded by the MRP (multidrug resistance associated protein) gene is induced by cisplatin in human leukemia HL-60/R-CP (cisplatin-resistant) cells and modulates cell growth inhibition by delta(7)-prostaglandin A1 (PGA1) methyl ester. The MRP mRNA level...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00744216
更新日期:1996-01-01 00:00:00
abstract::There is a constant and growing interest in exploitingadenoviruses as vectors for gene therapy when transientexpression of a therapeutic protein is necessary. Therequirement for an increased viral titre has prompted asearch for techniques by which this virus may be assayedwith greater speed and simplicity. Conventiona...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1021106116887
更新日期:2002-01-01 00:00:00
abstract::Specific respiration rate ( [Formula: see text]) is a key parameter to understand cell metabolism and physiological state, providing useful information for process supervision and control. In this work, we cultivated different insect cells in a very controlled environment, being able to measure [Formula: see text]. Sp...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-007-9118-8
更新日期:2008-05-01 00:00:00
abstract::The capacity of hepatocytes and cholangiocytes to contribute to their own maintenance has long been recognized. More recently, studies have indicated the presence of both intra-hepatic and extra-hepatic stem/progenitor cell populations. The intraorgan compartment probably derives primarily from the biliary tree, most ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1024826823194
更新日期:2003-03-01 00:00:00
abstract::Alkylating agents are the most widely used anticancer drugs whose main target is the DNA, although how exactly the DNA lesions cause cell death is still not clear. The emergence of resistance to this class of drugs as well as to other antitumor agents is one of the major causes of failure of cancer treatment. This pap...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008060720608
更新日期:1998-09-01 00:00:00
abstract:CONCLUSIONS:The BEVS has become widely utilized for production of recombinant proteins. However, protein aggregation and inefficient processing often limit yields, especially for secreted and membrane proteins. Since many proteins of pharmaceutical interest require similar posttranslational processing steps, engineerin...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00350396
更新日期:1996-01-01 00:00:00