High-level expression of a foreign gene by a recombinant baculovirus with an expanded host range.


:The usefulness of host range expanded viruses as an expressionvector system was investigated by following the expression ofthe E. coli lacZ gene. The host range expanded recombinantviruses were obtained from Sf-21 or BmN-4 cells coinfected withAutographa californica and Bombyx mori nuclearpolyhedrosis viruses. Among the host range expanded viruses,RecB-8 and RecS-B6 have similar enzyme digestion profiles butdifferent infection characteristics in cells. Therefore, tostudy the foreign gene expression efficiency of these twoviruses, we constructed recombinant viruses RecB8-LacZ andRecSB6-LacZ containing the lacZ gene instead of the polyhedringene. Also, the host range expanded recombinant AcNPV, Bac-BH,containing lacZ gene in the polyhedrin gene locus was constructedby substitution of the 0.6 kb region within the helicase gene ofBacPAK6 with that of BmNPV. beta-Galactosidase expressionefficiency by these viruses were determined and compared in Sf-21and BmN-4 cells. The result showed that Bac-BH has highexpression efficiency only in Sf-21 cells, whereas RecB8-LacZhas high expression efficiency both in Sf-21 and BmN-4 cells.Also, in BmN-4 cells, beta-galactosidase expressionefficiency of RecB8-LacZ was higher than that of recombinantBmNPV (BmK1-LacZ containing lacZ gene in polyhedrin gene locus).In addition, the expression efficiency was not correlated withvirus titer.






Kim HS,Woo SD,Kim WJ,Choi JY,Kang SK




Has Abstract


2000-02-01 00:00:00












  • Explants of porcine coronary artery in culture: A paradigm for studying the influence of heparin on vascular wall cell proliferation.

    abstract::Explant cultures of porcine coronary artery provided a coculture model, used as a paradigm of arterial wall in contact with vascular prosthesis which allowed the study of spatial and temporal changes in cell phenotype. First cells emerging from the explant had an endothelial phenotype monitored by cytoimmunostaining. ...


    pub_type: 杂志文章


    authors: Dufresne M,Warocquier-Clérout R

    更新日期:2001-09-01 00:00:00

  • The enhancement of growth and differentiation of rat adrenal nerve cells by the addition of conditioned medium from human fibroblast cultures.

    abstract::The growth of rat adrenal nerve cells was remarkably enhanced by supplementing the cultured medium from the human fibroblast cell line, Hs 68. Maximum specific growth rate and length of the neurites were observed as 0.076 (1/hr) and 0.026 mm, respectively in 20% supplement of five day old medium. In adding more than 2...


    pub_type: 杂志文章


    authors: Hong JS,Kim DS,Kim SH,Choi DH,Lee JH,Lee HY

    更新日期:1998-03-01 00:00:00

  • Uptake and efflux of FL118 and two FL118 derivatives in 3D cell model.

    abstract::Drug uptake and efflux are two of the critical factors required in order to be able to define drug efficacy. This study aims to investigate cytotoxicity and uptake mechanisms of two FL118 analogues (7-Q20 and val-FL118) in parallel with FL118 in three-dimensional multi-cellular spheroids model. The influence of compou...


    pub_type: 杂志文章


    authors: Zhou L,Weng Q,Zheng Y,Zhou Y,Li Q,Li F

    更新日期:2019-07-12 00:00:00

  • Assessment of the effect of TLR7/8, TLR9 agonists and CD40 ligand on the transformation efficiency of Epstein-Barr virus in human B lymphocytes by limiting dilution assay.

    abstract::Infection of human B cells with Epstein-Barr virus (EBV) induces polyclonal activation in almost all infected cells, but a small proportion of infected cells are transformed to immortalized lymphoblastoid cell lines. Since B cells are activated also by CD40 ligand (CD40L) and Toll-like receptor (TLR) agonists via a si...


    pub_type: 杂志文章


    authors: Younesi V,Shirazi FG,Memarian A,Amanzadeh A,Jeddi-Tehrani M,Shokri F

    更新日期:2014-01-01 00:00:00

  • Formation of multilayer rosettes in phytohemagglutinin-stimulated lymphocytes: correlation with chromatin condensation conformation in premature chromosome condensation.

    abstract::We found that the formation of multilayer rosettes by transformed human blood lymphocytes after phytohemagglutinin (PHA) stimulation is correlated with conformational changes of the chromatin as seen by premature chromosome condensation (PCC). The frequency distribution of grades of PCC and multilayer rosette formatio...


    pub_type: 杂志文章


    authors: Thomou H,Sambani C,Kitsiou P,Politis G

    更新日期:1988-07-01 00:00:00

  • Reduction of charge variants by CHO cell culture process optimization.

    abstract::Over the past decade, global interest in the development of therapeutic monoclonal antibodies (mAbs) has risen rapidly. As therapeutic agents, antibodies have shown marked efficacy in combatting a range of cancers and immune diseases with high target specificity and low toxicity (Carla Lucia et al. in PLoS ONE 6:e2407...


    pub_type: 杂志文章


    authors: Weng Z,Jin J,Shao C,Li H

    更新日期:2020-04-01 00:00:00

  • Effects of temperature shift on cell cycle, apoptosis and nucleotide pools in CHO cell batch cultues.

    abstract::Temperature reduction in CHO cell batch culture may be beneficial in the production of recombinant protein and in maintenance of viability. The effects on cell cycle, apoptosis and nucleotide pools were studied in cultures initiated at 37°C and temperature shifted to 30 °C after 48 hours. In control cultures maintaine...


    pub_type: 杂志文章


    authors: Moore A,Mercer J,Dutina G,Donahue CJ,Bauer KD,Mather JP,Etcheverry T,Ryll T

    更新日期:1997-01-01 00:00:00

  • A high-yielding serum-free, suspension cell culture process to manufacture recombinant adenoviral vectors for gene therapy.

    abstract::We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum free media and suspension growth. Subsequent...


    pub_type: 杂志文章


    authors: Schoofs G,Monica TJ,Ayala J,Horwitz J,Montgomery T,Roth G,Castillo FJ

    更新日期:1998-11-01 00:00:00

  • Dental pulp stem cells and osteogenesis: an update.

    abstract::Dental pulp stem cells constitute an attractive source of multipotent mesenchymal stem cells owing to their high proliferation rate and multilineage differentiation potential. Osteogenesis is initiated by osteoblasts, which originate from mesenchymal stem cells. These cells express specific surface antigens that disap...


    pub_type: 杂志文章,评审


    authors: Mortada I,Mortada R

    更新日期:2018-10-01 00:00:00

  • Atorvastatin neutralises the thrombin-induced tissue factor expresion in endothelial cells via geranylgeranyl pyrophosphate.

    abstract::Statins may have beneficial effects in atherogenesis given their antithrombotic properties involving non-lipid mechanisms that modify endothelial function of tissue factor induction by thrombin. In this study, we investigate the effect of atorvastatin on tissue factor (TF) activity in thrombin-stimulated endothelial c...


    pub_type: 杂志文章


    authors: Martínez-Sales V,Vila V,Ferrando M,Reganon E

    更新日期:2011-01-01 00:00:00

  • A practical approach for intracellular protein delivery.

    abstract::Protein delivery represents a powerful tool for experiments in live cells including studies of protein-protein interactions, protein interference with blocking antibodies, intracellular trafficking and protein or peptide biological functions. Most available reagents dedicated to the protein delivery allow efficient cr...


    pub_type: 杂志文章


    authors: Weill CO,Biri S,Adib A,Erbacher P

    更新日期:2008-01-01 00:00:00

  • Isolation, culture and identification of pulmonary arterial smooth muscle cells from rat distal pulmonary arteries.

    abstract::The culture of pulmonary arterial smooth muscle cells (PASMCs) is one of the most powerful tools for exploring the mechanisms of pulmonary hypertension (PH). Both pulmonary vasoconstriction and remodeling occur predominantly in distal pulmonary arteries (PA). In this study, we provide our detailed and standardized pro...


    pub_type: 杂志文章


    authors: Peng G,Xu J,Liu R,Fu Z,Li S,Hong W,Chen J,Li B,Ran P

    更新日期:2017-10-01 00:00:00

  • Comparison of specific rates of hybridoma growth and metabolism in batch and continuous cultures.

    abstract::For the mouse hybridoma cell line VO 208, kinetics of growth, consumption of glucose and glutamine, and production of lactate, ammonia and antibodies were compared in batch and continuous cultures. At a given specific growth rate, different metabolic activities were observed: a 40% lower glucose and glutamine consumpt...


    pub_type: 杂志文章


    authors: Goergen JL,Marc A,Engasser JM

    更新日期:1992-01-01 00:00:00

  • Development of a generic transient transfection process at 100 L scale.

    abstract::We have developed a generic transient transfection process at 100 L scale, using HEK293-EBNA cells and PEI as the transfection reagent for the production of recombinant IgG. The process, including large-scale plasmid preparation, expression at bioreactor scale, capture, purification and, if necessary, endotoxin remova...


    pub_type: 杂志文章


    authors: Tuvesson O,Uhe C,Rozkov A,Lüllau E

    更新日期:2008-02-01 00:00:00

  • Early detection of cell metabolism with a silicon microsensor.

    abstract::A silicon microsensor (ISFET - Ion Sensitive Field Effect Transistor) has been used to detect the metabolism of a cell population cultured on a coverslip and positioned close to the sensor surface. The system output is analyzed as a function of cell density. ...


    pub_type: 杂志文章


    authors: Martinoia S,Meloni M,Parodi MT,Tedesco M,Ciccarelli C,Grattarola M

    更新日期:1993-01-01 00:00:00

  • Electroporation: an arsenal of application.

    abstract::Electroporation is a way to induce nanometersized membrane pore for exogenous substances delivery into cytoplasm using an artificial electric field. Now it was widely used for molecules transfer especially in molecular experiments and genetic aspects. In recent years, modern electroporation on the embryo was developed...


    pub_type: 杂志文章


    authors: Yuan TF

    更新日期:2007-06-01 00:00:00

  • Xanthoria elegans (Link) (lichen) extract counteracts DNA damage and oxidative stress of mitomycin C in human lymphocytes.

    abstract::Several lichen species have been used for medicinal purposes throughout the ages, and they are reported to be effective in the treatment of different disorders including ulcer and cancer. It is revealed that lichens may be easily accessible sources of natural drugs and possible food supplements after their safety eval...


    pub_type: 杂志文章


    authors: Turkez H,Aydin E,Aslan A

    更新日期:2012-12-01 00:00:00

  • Biological response of endothelial cells and its modulation by cytokines: prospects for therapy and bioprocesses.

    abstract::Endothelial cells are involved in important pathological situations. They could be the target for infectious processes as for example in Cowdriosis, an important disease in cattle due to the rickettsia Cowdria ruminantium prevalent in the south of the Sahara. They are also connected to angiogenic processes related to ...


    pub_type: 杂志文章


    authors: Marique T,Blankaert D,Hendrick V,Raschella A,Declerck B,Alloin C,Teixera-Guerra I,Sandron D,Cherlet M,Parent D,Kirkpatrick C,Van Vooren JP,Wérenne J

    更新日期:1997-11-01 00:00:00

  • Expression of anchorin CII mRNA by cultured chondrocytes.

    abstract::The establishment of a cell culture system promoting chondrocyte differentiation has been utilized to better characterize phenotypic stages of chondrogenesis at the cellular level. Although the expression of the type II collagen gene has been studied during "in vitro" chondrocyte differentiation, little is known about...


    pub_type: 杂志文章


    authors: Castagnola P,Cancedda R

    更新日期:1991-02-01 00:00:00

  • Monoclonal antibodies against naturally occurring bioactive compounds.

    abstract::The ratio of hapten to bovine serum albumin (BSA) in an antigen conjugate was determined by matrix-assisted laser desorption/ionization (MALDI) tof mass spectrometry. A hybridoma secreting monoclonal antibody (MAb) was produced by fusing splenocytes immunized with an antigen-BSA conjugate with HAT-sensitive mouse myel...


    pub_type: 杂志文章


    authors: Shoyama Y,Tanaka H,Fukuda N

    更新日期:1999-09-01 00:00:00

  • Regulation of matrix metalloproteinase-1 and alpha-smooth muscle actin expression by interleukin-1 alpha and tumour necrosis factor alpha in hepatic stellate cells.

    abstract::Hepatic stellate cells (HSCs) are key players in liver fibrosis and regeneration via collagen degradation and synthesis. These phenomena involve inflammatory cytokines released from non-parenchymal liver cells such as Kupffer cells. Although the effects of individual cytokines on many cell types have been investigated...


    pub_type: 杂志文章


    authors: Inoue A,Obayashi K,Sonoda Y,Nakamura A,Ueno T,Kuhara S,Tashiro K

    更新日期:2017-06-01 00:00:00

  • The role of Sox9 in maintaining the characteristics and pluripotency of Arbas Cashmere goat hair follicle stem cells.

    abstract::In our previous work, we isolated Arbas Cashmere goat hair follicle stem cells (gHFSCs) and explored the pluripotency. In this study, we investigated the expression and putative role of Sox9 in the gHFSCs. Immunofluorescence staining showed that Sox9 is predominantly expressed in the bulge region of the Arbas Cashmere...


    pub_type: 杂志文章


    authors: He N,Dong Z,Tai D,Liang H,Guo X,Cang M,Liu D

    更新日期:2018-08-01 00:00:00

  • Mathematical models for multidrug resistance and its reversal.

    abstract::Mathematical models describing drug resistance are briefly reviewed. One model which describes the molecular function of the P-glycoprotein pump in multidrug resistant (MDR) cell lines has been developed and is presented in detail. The pump is modeled as an energy dependent facilitated diffusion process. A partial dif...


    pub_type: 杂志文章,评审


    authors: Michelson S

    更新日期:1993-01-01 00:00:00

  • Detection of mycoplasma contaminations by the polymerase chain reaction.

    abstract::The polymerase chain reaction (PCR) has been used for the general detection of Mollicutes. 25 Mycoplasma and Acholeplasma species were detected including important contaminants of cell cultures such as M. orale, M. arginini, M. hyorhinis, M. fermentans, A. laidlawii and additional human and animal mycoplasmas. PCR rea...


    pub_type: 杂志文章


    authors: Wirth M,Berthold E,Grashoff M,Pfützner H,Schubert U,Hauser H

    更新日期:1994-01-01 00:00:00

  • A speedy method to detect inserted DNA fragment in cell lines transfected with retroviral vectors.

    abstract::Cells transfected by retroviral vectors are brought in agene of particular interest and are very useful in avariety of experiments. It is essential to testify that theDNA fragment was successfully introduced into the cellstogether with the retroviral vectors. Polymerase chainreaction is believed to be a fast and conve...


    pub_type: 杂志文章


    authors: Ding LH,Iimura E,Saijo K,Hamada H,Ohno T

    更新日期:2000-11-01 00:00:00

  • Primary adipocyte culture: adipocyte purification methods may lead to a new understanding of adipose tissue growth and development.

    abstract::In the present manuscript, the methods required to generate purified cultures of mature adipocytes, as well as stromal vascular cells, from the same isolation are detailed. Also, we describe the in vitro conditions for the dedifferentiation of the isolated mature adipocytes. These two types of cells may be used to ree...


    pub_type: 杂志文章


    authors: Fernyhough ME,Vierck JL,Hausman GJ,Mir PS,Okine EK,Dodson MV

    更新日期:2004-10-01 00:00:00

  • Small interfering RNA knockdown of mini-TyrRS and mini-TrpRS effects angiogenesis in human umbilical vein endothelial cells in hypoxic culture.

    abstract::Aim We studied the role of mini-TyrRS and mini-TrpRS in angiogenesis by using small interfering RNA-mediated mini-TyrRS/mini-TrpRS knockout in hypoxic culture of human umbilical vein endothelial cells. Methods SiRNA was used as the main method to inhibited the gene function. Silencing efficiency was assayed by real-ti...


    pub_type: 杂志文章


    authors: Zeng R,Chen YC,Zeng Z,Liu R,Qiang O,Jiang XF,Liu XX,Li X,Wang HY

    更新日期:2008-03-01 00:00:00

  • Enhanced secretory ability for the human factor VIII light chain produced in baculovirus-infected insect cells.

    abstract::The light and truncated heavy chains of human factor VIII, expressed separately in baculovirus-infected insect cells, exhibited different secretory behaviour when compared with each other and with a biologically active fusion molecule of the truncated heavy and light chains. The light chain was very efficiently secret...


    pub_type: 杂志文章


    authors: Webb E,Tkalcevic J,Edwards S,Hocking D,Nisbet I

    更新日期:1996-01-01 00:00:00

  • Photobiomodulation effects on osteogenic differentiation of adipose-derived stem cells.

    abstract::Increasing interest has been observed in the use of photobiomodulation (PBM) to enhance the proliferation of stem cells and induce their differentiation. The effects of PBM at two different wavelengths (635 and 809 nm) with three different energy densities (0.5, 1 and 2 J/cm2) on the osteogenic differentiation of adip...


    pub_type: 杂志文章


    authors: Bölükbaşı Ateş G,Ak A,Garipcan B,Gülsoy M

    更新日期:2020-04-01 00:00:00

  • Cell cycle, cell size and mitochondrial activity of hybridoma cells during batch cultivation.

    abstract::Cell cycle, cell size and rhodamine 123 fluorescence in cell populations of two batch cultures were analysed and quantified with a fluorescence-activated cell sorter (FACS). Two cultures derived from either exponential or stationary phase innocula were investigated in order to demonstrate the dependency of the subsequ...


    pub_type: 杂志文章


    authors: al-Rubeai M,Chalder S,Bird R,Emery AN

    更新日期:1991-11-01 00:00:00