Estimation of rates of oxygen uptake and carbon dioxide evolution of animal cell culture using material and energy balances.

Abstract:

:Material and degree of reductance balance equations are used to estimate the rates of oxygen uptake and carbon dioxide evolution of animal cell cultures. Lumped compositions, molecular weight and reductance degree of cellular protein, monoclonal antibody, biomass and amino acid consumption (excluding glutamine and alanine) are found to be relatively constant for different hybridoma cell lines and may be used as regularities. The calculated rates of oxygen uptake and carbon dioxide evolution agree well with experimental values of several different cultures reported in the literature. This simple method gives the same results as calculated on the basis of a detailed metabolic reaction network.

journal_name

Cytotechnology

journal_title

Cytotechnology

authors

Xiu ZL,Deckwer WD,Zeng AP

doi

10.1023/A:1008004618163

subject

Has Abstract

pub_date

1999-05-01 00:00:00

pages

159-66

issue

3

eissn

0920-9069

issn

1573-0778

journal_volume

29

pub_type

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