Anti-allergic triterpenes isolated from olive milled waste.

abstract：:In previous studies, vascular smooth muscle progenitor cells (vSMPCs) isolated from peripheral blood mononuclear cells (PBMCs) were cultured using medium containing platelet-derived growth factor-BB (PDGF-BB) for 4 weeks. However, this method requires long culture periods of up to 4 weeks and yields low cell counts. T...

journal_title：Cytotechnology

authors： Seong JH,Song YS,Joo HW,Park IH,Shen GY,Shin NK,Lee AH,Kwon AM,Lee Y,Kim H,Kim KS

更新日期：2020-10-01 00:00:00

abstract：:Drug uptake and efflux are two of the critical factors required in order to be able to define drug efficacy. This study aims to investigate cytotoxicity and uptake mechanisms of two FL118 analogues (7-Q20 and val-FL118) in parallel with FL118 in three-dimensional multi-cellular spheroids model. The influence of compou...

journal_title：Cytotechnology

authors： Zhou L,Weng Q,Zheng Y,Zhou Y,Li Q,Li F

更新日期：2019-07-12 00:00:00

abstract：:High density cell culture systems offer the advantage of production of bio-pharmaceuticals in compact bioreactors with high volumetric production rates; however, these systems are difficult to design and operate. First of all, the cells have to be retained in the bioreactor by physical means during perfusion. The desi...

journal_title：Cytotechnology

authors： Ozturk SS

更新日期：1996-01-01 00:00:00

abstract：:The cancer stem cell hypothesis posits that tumors are derived from a single cancer-initiating cell with stem cell properties. The task of identifying and characterizing cancer-initiating cells with stem cell properties at the single cell level has proven technically difficult because of the scarcity of the cancer ste...

journal_title：Cytotechnology

authors： Cocola C,Sanzone S,Astigiano S,Pelucchi P,Piscitelli E,Vilardo L,Barbieri O,Bertoli G,Reinbold RA,Zucchi I

更新日期：2008-09-01 00:00:00

abstract：:Many cytological studies have reported that the numbers of binucleated cells were elevated in various tumors. However, binucleated cells are observed in not only malignant tumors but also normal tissues. Thus, the clinical significance of binucleated cells is controversial. Here we attempted to elucidate the character...

journal_title：Cytotechnology

authors： Nishimura K,Watanabe S,Hayashida R,Sugishima S,Iwasaka T,Kaku T

更新日期：2016-08-01 00:00:00

abstract：:By employing the affinity gel fraction technique, we have detected a 175 kDa tyrosine-O-sulfate (TyrS)-binding protein in sodium choleate extracts of the microsomal membrane fractions of bovine liver and pancreas, as well as canine liver and pancreas. Western blot analysis revealed the presence of the bovine liver Tyr...

journal_title：Cytotechnology

authors： Liu MC,Sakakibara Y,Suiko M

更新日期：1997-01-01 00:00:00

abstract：:Numerous challenges remain in the successful clinical translation of cell-based therapeutic studies for skeletal tissue repair, including appropriate cell sources and viable cell delivery systems. Poly(ethylene glycol)-poly(ε-caprolactone) (PEG-PCL) amphiphilic block copolymers have been extensively explored in micros...

journal_title：Cytotechnology

authors： Zhang D,Tong A,Zhou L,Fang F,Guo G

更新日期：2012-12-01 00:00:00

abstract：:Carvacrol, the predominant monoterpene in many essential oils of Labitae including Origanum, Satureja, Thymbra, Thymus, and Corydothymus has substantial antibacterial, antifungal, antihelmintic, insecticial, analgesic and antioxidant activities. Approximately 75% of lung cancer is non-small cell carcinoma (NSCLC) whic...

journal_title：Cytotechnology

authors： Koparal AT,Zeytinoglu M

更新日期：2003-11-01 00:00:00

abstract：:The bone marrow represents the most common source from which to isolate mesenchymal stem cells (MSCs). MSCs are capable of differentiating into tissues of the three primary lineages and have the potential to enhance repair in damaged organs through the principals of regenerative medicine. Given the ease with which MSC...

journal_title：Cytotechnology

authors： Dickinson H,Milton P,Jenkin G

更新日期：2012-10-01 00:00:00

abstract：:Multidrug resistance (MDR) is a major problem in cancer chemotherapy. As P-glycoprotein is the key molecule in MDR, many investigators have constructed anti-P-glycoprotein monoclonal antibodies (MAbs). Those antibodies, including MRK16 and C219, were used for elucidation of the mechanism of MDR and for overcoming of M...

journal_title：Cytotechnology

authors： Heike Y,Tsuruo T

更新日期：1993-01-01 00:00:00

abstract：:The cytotoxic necrotizing factor type 1 (CNF1) fromE. coli causes necrosis in rabbit skin and multinucleation in cultured cells. Cells exposed to CNF1 were characterized by changes in actin organization, mainly consisting in the presence of well-developed stress fibers and membrane ruffles. The interaction of CNF1 wit...

journal_title：Cytotechnology

authors： Falzano L,Fiorentini C,Boquet P,Donelli G

更新日期：1993-01-01 00:00:00

abstract：:A water soluble extract from the bark of the Samoan medicinal plant Alphitonia zizyphoides A. Gray (Rhamnaceae), enhances the plating efficiency in vitro of lymphoid cell lines as well as the survival of bone marrow cells and normal T and B lymphocytes. Furthermore, the inclusion of bark-extract into culture media enh...

journal_title：Cytotechnology

authors： Dunstan CA,Andersson J,Bohlin L,Cox PA,Grönvik KO

更新日期：1994-01-01 00:00:00

abstract：:We provide evidence that the expression of the human MRP/GS-X pump encoded by the MRP (multidrug resistance associated protein) gene is induced by cisplatin in human leukemia HL-60/R-CP (cisplatin-resistant) cells and modulates cell growth inhibition by delta(7)-prostaglandin A1 (PGA1) methyl ester. The MRP mRNA level...

journal_title：Cytotechnology

authors： Akimaru K,Kuo MT,Furuta K,Suzuki M,Noyori R,Ishikawa T

更新日期：1996-01-01 00:00:00

abstract：:The growth of rat adrenal nerve cells was remarkably enhanced by supplementing the cultured medium from the human fibroblast cell line, Hs 68. Maximum specific growth rate and length of the neurites were observed as 0.076 (1/hr) and 0.026 mm, respectively in 20% supplement of five day old medium. In adding more than 2...

journal_title：Cytotechnology

authors： Hong JS,Kim DS,Kim SH,Choi DH,Lee JH,Lee HY

更新日期：1998-03-01 00:00:00

abstract：:It is well known that the characteristics of cell lines possibly alter when cell lines are at high-passage number because of the environmental selection. We do not know whether non-permissive or low-permissive cell lines could become permissive or more permissive to virus infection after over-high passage. In the pres...

journal_title：Cytotechnology

authors： Zhang X,Lan W,Deng Y,Ma Y,Liu K,Peng J,Li Y,Hong H

更新日期：2008-07-01 00:00:00

abstract：:Megalocytiviruses are important emerging pathogens in both freshwater and marine finfish aquaculture. However, a limited number of piscine cell lines are persistently susceptible to these viruses, which greatly limits the study of megalocytiviruses. In this study, a new fibroblast-like cell line was established from a...

journal_title：Cytotechnology

authors： Dong C,Shuang F,Weng S,He J

更新日期：2014-12-01 00:00:00

abstract：:The statistics of estimators used with the endpoint assay for virus titration were investigated. For a standard assay with 10 wells/dilution, the graphical estimator traditionally used was found to produce estimates with significant positive bias and a relatively low accuracy. Furthermore, the graphical estimator was ...

journal_title：Cytotechnology

authors： Nielsen LK,Smyth GK,Greenfield PF

更新日期：1992-01-01 00:00:00

abstract：:Statins may have beneficial effects in atherogenesis given their antithrombotic properties involving non-lipid mechanisms that modify endothelial function of tissue factor induction by thrombin. In this study, we investigate the effect of atorvastatin on tissue factor (TF) activity in thrombin-stimulated endothelial c...

journal_title：Cytotechnology

authors： Martínez-Sales V,Vila V,Ferrando M,Reganon E

更新日期：2011-01-01 00:00:00

abstract：:Disabled Infectious Single Cycle (DISC) HSV-2 has been cultured in the complimentary cell line CR2 to provide high titre bulk material suitable for the purification of the virus as a live viral vaccine. CR2 cells are cultured on the microcarrier Cytodex-1 at 5 g l-1 in small scale (1 l) and larger scale (15 l) reactor...

journal_title：Cytotechnology

authors： Zecchini TA,Smith RJ

更新日期：1999-07-01 00:00:00

abstract：:To construct a recombinant protein highly producing cell lines, we have previously developed the Oncogene Activated Production (OAP) system by using BHK-21 cells. Here we verified the availability of the OAP system in CHO cells. We firstly generated 'primed' ras amplified CHO cells, ras clone I, by introducing human c...

journal_title：Cytotechnology

authors： Katakura Y,Seto P,Miura T,Ohashi H,Teruya K,Shirahata S

更新日期：1999-09-01 00:00:00

abstract：:With the increasingly competitive commercial production of target proteins by hybridoma and genetically engineered cells, there is an urgent requirement for biosensors to monitor and control on-line and in real time the growth of cultured cells. Since growth is accompanied by an enthalpy change, heat dissipation measu...

journal_title：Cytotechnology

authors： Guan YH,Kemp RB

更新日期：1999-07-01 00:00:00

abstract：:Various parts of Mimusops elengi Linn. (Sapotaceae) have been used widely in traditional Indian medicine for the treatment of pain, inflammation and wounds. The study was conducted to explore the use of stem bark of M. elengi on pharmacological grounds and to evaluate the scientific basis of cytotoxic and anti-tumor a...

journal_title：Cytotechnology

authors： Kumar H,Savaliya M,Biswas S,Nayak PG,Maliyakkal N,Manjunath Setty M,Gourishetti K,Pai KS

更新日期：2016-08-01 00:00:00

abstract：:Galangin, an active flavonoid present at high concentration in Alpinia officinarum Hance and propolis, shows cytotoxicity towards several cancer cell lines, including melanoma. However, the specific cellular targets of galangin-induced cytotoxicity in melanoma are still unknown. Here, we investigated the effects of ga...

journal_title：Cytotechnology

authors： Zhang W,Lan Y,Huang Q,Hua Z

更新日期：2013-05-01 00:00:00

abstract：:Dental pulp stem cells constitute an attractive source of multipotent mesenchymal stem cells owing to their high proliferation rate and multilineage differentiation potential. Osteogenesis is initiated by osteoblasts, which originate from mesenchymal stem cells. These cells express specific surface antigens that disap...

journal_title：Cytotechnology

authors： Mortada I,Mortada R

更新日期：2018-10-01 00:00:00

abstract：:Sorbaria kirilowii (Regel) Maxim, a plant found in China, Korea, Japan, and east of Europe, is a common herb used for traditional medicinal purposes. However, its ability to prevent photoaging has not been studied. In this study, we investigated the anti-photoaging functions of an ethanol extract (Sk-EE) of S. kirilow...

journal_title：Cytotechnology

authors： Chen H,Jang J,Kopalli SR,Yum J,Yoon K,Cho JY

更新日期：2021-02-01 00:00:00

abstract：:In the last 10 years, new assignments and the special demands of mammalian cells to the culture conditions caused the development of complex small scale fermentation setups. The use of continuous fermentation and cell retention devices requires appropriate process control systems. An arrangement for control and data-a...

journal_title：Cytotechnology

authors： Büntemeyer H,Marzahl R,Lehmann J

更新日期：1994-01-01 00:00:00

abstract：:Parkinson's disease (PD) is one of the most common neural degenerative disease, affecting millions of people globally. Great progress has been made in the PD treatment, and one of the most promising one is the stem cell-based therapy. Thus, studies on the differentiation of neural stem cells (NSCs) are important to th...

journal_title：Cytotechnology

authors： Qi L,Tang Y,He W,Pan H,Jiang W,Wang L,Deng W

更新日期：2017-04-01 00:00:00

abstract：:The potential of spent medium to support the growth and recombinant protein production of High-Fivetrade mark cells was investigated. Growth in medium consisting of three parts fresh and one part spent medium was comparable to that in fresh medium (maximal specific growth rates of 0.028 and 0.029 h(-1), and maximal ce...

journal_title：Cytotechnology

authors： Ikonomou L,Bastin G,Schneider YJ,Agathos SN

更新日期：2004-01-01 00:00:00

abstract：:This paper reviews the JRC-Ispra research activity on toxicological studies related to the environmental and occupational exposure of trace metals, as carried out by a combination of cell culture methodologies with nuclear and radioanalytical techniques.Applications concern the setting of uptake-effect relationships a...

journal_title：Cytotechnology

authors： Sabbioni E,Pozzi G,Fischbach M,Bahbouth E

更新日期：1993-01-01 00:00:00

abstract：:This article describes the current status in the development of human monoclonal antibodies. Over the last ten years a lot of information about the human immune system has emerged. Combining these with the many new (bio-)technologies it is plausible that the long awaited breakthrough of this technology is close. This ...

journal_title：Cytotechnology

authors： Lindl T

更新日期：1996-01-01 00:00:00