Effects of Thymus kotschyanus var. glabrescens Boiss. extract on mitomycin-C induced chromosomal aberrations and sister chromatid exchanges in human lymphocytes.

Abstract:

:In this investigation, clastogenic effects of Thymus kotschyanus var. glabrescens Boiss. extract (TE) and anticlastogenic effects of this extract against Mitomycin C (MMC) induced chromosome damage have been evaluated in human peripheral blood lymphocytes in vitro. Two series of experiments were conducted. In the first, only 10(-5), 10(-4), 10(-3) and 10(-2) mul ml(-1) concentrations of TE were used for 48 h to detect potential clastogenicity. In the second, MMC (0.38 mug ml(-1)) plus 10(-5), 10(-4), 10(-3) and 10(-2) mul ml(-1) concentrations of TE were used for 48 h to determine anticlastogenic effects. TE did not increase sister chromatid exchanges (SCEs) (except 10(-2) mul ml concentration) and chromosome aberrations (CAs) significantly compared with negative and solvent controls. However, it decreased the frequency of MMC induced chromosome aberrations. Decreasing was significant at 10(-4), 10(-3) and 10(-2) mul ml(-1) concentrations. On the other hand, TE significantly increased MMC-induced SCEs for all treatment groups compared with positive control.

journal_name

Cytotechnology

journal_title

Cytotechnology

authors

Celik M,Unal F,Yüzbaşıoğlu D,Yılmaz S,Aksoy H,Karaman S

doi

10.1007/s10616-006-9015-6

subject

Has Abstract

pub_date

2006-06-01 00:00:00

pages

99-104

issue

2

eissn

0920-9069

issn

1573-0778

journal_volume

51

pub_type

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