Abstract:
:Monoclonal antibodies (mAb) are high added value glycoproteins recommended for immunotherapy, diagnosis, and also for the treatment of bacterial infections resistant to multiple drugs such as Methicillin Resistant Staphylococcus aureus (MRSA). In addition to environmental conditions related to cell cultures, the intrinsic characteristics of hybridoma cells, like the secretion stability of monoclonal antibodies by the cells through successive subcultures, are relevant for the characterization of cell lines related to the productivity of mAb. The rate of mAb production differs significantly between different cell lines and different passage numbers, and it is an important variable in characterization of cell lines. In order to find a more robust, faster-growing, and higher-productivity cell line of hybridoma, cultivations in 24-well plates were performed in different subculture periods, or cell passages (P), of hybridoma cells producing MRSA anti-PBP2a monoclonal antibodies [MRSA-antiPBP2a (mAb)]. The objective of this study was to study the effects of cell growth and production of MRSA-antiPBP2a mAb secreted by murine hybridoma cells grown in different passages as well as determine the which passages the hybridomas can be cultivated without harming their growth and productivity. So, cell growth profiles of hybridomas secreting MRSA-antiPBP2a (mAb) and the production of MRSA-antiPBP2a mAb in different subculture periods or cell passages (P) were studied. Cell growth tests, monoclonal antibody productivity, and metabolite characteristics revealed substantial differences in those cells kept between P10 and P50. Similarities in the secretion of monoclonal antibody, growth, and metabolic profiles, were noted in the MRSA-antiPBP2a mAb producing hybridoma cells kept between P10 and P20. Also, glucose consumption (g/L) and lactate production (g/L) in the latter cell cultures were monitored daily through biochemical analyzer. As of P30, it was observed a 4.4 times reduction in productivity, a 13 % reduction in metabolic yield, and a significant change in cell growth. Secretion of MRSA-antiPBP2a mAb should be obtained through the culture of hybridomas up to P20 in order to keep its stability.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Corrêa AL,Senna JP,de Sousa ÁPdoi
10.1007/s10616-014-9794-0subject
Has Abstractpub_date
2016-05-01 00:00:00pages
419-27issue
3eissn
0920-9069issn
1573-0778pii
10.1007/s10616-014-9794-0journal_volume
68pub_type
杂志文章相关文献
CYTOTECHNOLOGY文献大全abstract::Large scale animal cell culture for the production of complex therapeutic proteins has been a major success of the biotechnology industry. Today, approximately half of the $ 5 billion annual turnover of the biotechnology industry is based upon this technology, in many cases with reactors of more than 10 m(3). As we lo...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00744313
更新日期:1995-01-01 00:00:00
abstract::The capacity of hepatocytes and cholangiocytes to contribute to their own maintenance has long been recognized. More recently, studies have indicated the presence of both intra-hepatic and extra-hepatic stem/progenitor cell populations. The intraorgan compartment probably derives primarily from the biliary tree, most ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1024826823194
更新日期:2003-03-01 00:00:00
abstract::The objective of this study was to compare effects of in vitro culture systems on embryonic development and expression patterns of developmentally important genes in preimplantation buffalo embryos. After IVM/IVF presumptive zygotes were cultured in one of three systems: undefined TCM-199, mCR2aa medium supplemented w...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-016-0010-2
更新日期:2016-10-01 00:00:00
abstract::Oral tolerance is the systemic immunological unresponsiveness that occurs after feeding protein antigens. Its physiological role is thought to be the prevention of hypersensitivity to food antigens, and its therapeutic use to treat inflammatory diseases has been suggested. Although it has been shown that CD4(+) T cell...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/b:cyto.0000039918.80472.0e
更新日期:2003-11-01 00:00:00
abstract::Human herpes virus 6 (HHV-6) is a common human pathogen that is most often detected in hematopoietic cells. Although human cells harboring chromosomally integrated HHV-6 can be generated in vitro, the availability of such cell lines originating from in vivo tissues is limited. In this study, chromosomally integrated H...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-017-0119-y
更新日期:2018-02-01 00:00:00
abstract::Benfuracarb is a carbamate insecticide used to control insect pests in vegetables and it has anti-acetylcholinesterase activity lower than other carbamates. Cytotoxic effects of benfuracarb were evaluated by using root growth inhibition (EC50), mitotic index (MI), and mitotic phase determinations on the root meristem ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-014-9811-3
更新日期:2016-08-01 00:00:00
abstract::Human skin has continuous self-renewal potential throughout adult life and serves as first line of defence. Its cellular components such as human epidermal keratinocytes (HEKs) and dermal mesenchymal stromal cells (DMSCs) are valuable resources for wound healing applications and cell based therapies. Here we show a si...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-015-9857-x
更新日期:2016-08-01 00:00:00
abstract::The effects of media concentrations of glucose andglutamine on the intracellular nucleotide pools andoxygen uptake rates of a murine antibody-secretinghybridoma cell line were investigated. Cells takenfrom mid-exponential phase of growth were incubated inmedium containing varying concentrations of glucose(0-25 mM) and...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008154615643
更新日期:2000-10-01 00:00:00
abstract::Prostacyclin alternatively called prostaglandin (PG) I2 is an unstable metabolite synthesized by the arachidonate cyclooxygenase pathway. Earlier studies have suggested that prostacyclin analogues can act as a potent effector of adipose differentiation. However, biosynthesis of PGI2 has not been determined comprehensi...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-013-9616-9
更新日期:2014-08-01 00:00:00
abstract::Various parts of Mimusops elengi Linn. (Sapotaceae) have been used widely in traditional Indian medicine for the treatment of pain, inflammation and wounds. The study was conducted to explore the use of stem bark of M. elengi on pharmacological grounds and to evaluate the scientific basis of cytotoxic and anti-tumor a...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-014-9839-4
更新日期:2016-08-01 00:00:00
abstract::This study demonstrated a newly developed method using adipose tissue-derived stromal cells (ADSCs) and hydroxypropylmethylcellulose (HPMC) in building injectable tissue engineered cartilage in vivo. ADSCs from rabbit subcutaneous fatty tissue were cultured in chondrogenic differentiation medium and supplemented with ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-013-9627-6
更新日期:2014-10-01 00:00:00
abstract::A silicon microsensor (ISFET - Ion Sensitive Field Effect Transistor) has been used to detect the metabolism of a cell population cultured on a coverslip and positioned close to the sensor surface. The system output is analyzed as a function of cell density. ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00746064
更新日期:1993-01-01 00:00:00
abstract::The Atlantic salmon (Salmo salar) serum lectin (SSL) is a soluble C-type lectin that binds bacteria, including salmon pathogens. This lectin is a cysteine-rich oligomeric protein. Consequently, a Drosophila melanogaster expression system was evaluated for use in expressing SSL. A cDNA encoding SSL was cloned into a ve...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-012-9505-7
更新日期:2013-08-01 00:00:00
abstract::To construct a recombinant protein highly producing cell lines, we have previously developed the Oncogene Activated Production (OAP) system by using BHK-21 cells. Here we verified the availability of the OAP system in CHO cells. We firstly generated 'primed' ras amplified CHO cells, ras clone I, by introducing human c...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008048928053
更新日期:1999-09-01 00:00:00
abstract::The cancer stem cell hypothesis posits that tumors are derived from a single cancer-initiating cell with stem cell properties. The task of identifying and characterizing cancer-initiating cells with stem cell properties at the single cell level has proven technically difficult because of the scarcity of the cancer ste...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-008-9173-9
更新日期:2008-09-01 00:00:00
abstract::Sufentanil, a lipophilic opioid, is the most frequently used clinical drug for ischemic heart disease. The effects of sufentanil on MAPK signaling in ischemic heart disease were explored. The effects of sufentanil on ischemia-reperfusion (IR)-induced myocardial injury in a rat model were examined. The serum levels of ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-017-0127-y
更新日期:2018-02-01 00:00:00
abstract::Over the past decade, global interest in the development of therapeutic monoclonal antibodies (mAbs) has risen rapidly. As therapeutic agents, antibodies have shown marked efficacy in combatting a range of cancers and immune diseases with high target specificity and low toxicity (Carla Lucia et al. in PLoS ONE 6:e2407...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-020-00375-x
更新日期:2020-04-01 00:00:00
abstract::Fusion of lymphoblastoid cell lines that produce human monoclonal antibodies against Pseudomonas aeruginosa with the human/mouse heteromyeloma SHM-D33 generated heterohybrids that were stable and secreted antibody in the range of 20 to 300 micrograms/ml. One of the hybridoma cell lines ws adapted to serum-free medium ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00365266
更新日期:1990-01-01 00:00:00
abstract::IL-5 plays important roles in eosinophil differentiation, expansion, and recruitment. The regulation of IL-5 seems critical for the treatment of eosinophil-mediated allergic reactions. However, the precise mechanisms for IL-5 regulation remain unknown. In this study, we investigated how IL-5 production is regulated. T...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-013-9585-z
更新日期:2014-05-01 00:00:00
abstract::The benzofuran lignans egonol and homoegonol are found in all species of the genus Styrax. Since natural products are important sources of new anticancer drugs, this study evaluated the cytotoxic activity of a hydroalcoholic extract of the stems of S. camporum (SCHE) and their chemical markers, egonol (EG) and homoego...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-015-9864-y
更新日期:2016-08-01 00:00:00
abstract::Hypertension (HT) is a common and life threating health problem worldwide leading to stroke, heart attack and renal failure. It is characterized by elevated blood pressure forced heart load. Human interleukin-6 (IL-6) and C- reactive protein (CRP) are known to be involved in inflammatory processes. IL-6 gene is a poly...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-014-9732-1
更新日期:2015-12-01 00:00:00
abstract::The efficacy of hepatocellular carcinoma (HCC) treatment is very low because of the high percentage of recurrence and resistance to anticancer agents. Hepatic cancer stem cells (HCSCs) are considered the origin of such recurrence and resistance. Our aim was to evaluate the stemness of doxorubicin and 5-fluorouracil re...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-012-9511-9
更新日期:2013-08-01 00:00:00
abstract::Vascular growth factors, including vascular endothelial growth factor and fibroblast growth factor-2, bind to heparan sulfate proteoglycans in the basement membrane. While this binding, storage, and release system provides a critical model for controlled drug release devices, basement membrane-growth factor binding ki...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-009-9212-1
更新日期:2009-07-01 00:00:00
abstract::Recent evidence from molecular cloning, biochemical and immunological experiments has established that the cation-independent mannose-6-phosphate (Man-6-P) receptor and insulin-like growth factor-II (IGF-II) receptor are the same protein. Although the role of the IGF-II/Man-6-P receptor as a transporter of hydrolytic ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00364994
更新日期:1989-12-01 00:00:00
abstract::The statistics of estimators used with the endpoint assay for virus titration were investigated. For a standard assay with 10 wells/dilution, the graphical estimator traditionally used was found to produce estimates with significant positive bias and a relatively low accuracy. Furthermore, the graphical estimator was ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF02522040
更新日期:1992-01-01 00:00:00
abstract::Infection of human B cells with Epstein-Barr virus (EBV) induces polyclonal activation in almost all infected cells, but a small proportion of infected cells are transformed to immortalized lymphoblastoid cell lines. Since B cells are activated also by CD40 ligand (CD40L) and Toll-like receptor (TLR) agonists via a si...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-013-9542-x
更新日期:2014-01-01 00:00:00
abstract::Gingival fibroblast cultures from four patients with Down's Syndrome (DS) and periodontal disease were compared with four in vitro age-matched fibroblast cultures of handicapped subjects (ND) also affected by periodontitis. The extra copy of chromosome 21 could alter growth regulation and biochemical mechanisms, so we...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::Mandibular molar anlages excised from 17-day mouse foetuses were cultured in vitro or in ovo (on the chorioallantoic membrane). In both cases, the explants were underlain either with a Millipore filter or with a piece of fibrin foam. Tooth germs were harvested after 7 days of cultivation and processed histologically. ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00135638
更新日期:1991-09-01 00:00:00
abstract::Mitochondrial dysfunction has been identified as one of the primary factors contributing to liver diseases. Pathways that control mitochondrial biogenesis are potential therapeutic targets for the amelioration of hepatocyte dysfunction and liver disease. Research on natural pharmacological agents that ameliorate liver...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-018-0242-4
更新日期:2018-12-01 00:00:00
abstract::Alkylating agents are the most widely used anticancer drugs whose main target is the DNA, although how exactly the DNA lesions cause cell death is still not clear. The emergence of resistance to this class of drugs as well as to other antitumor agents is one of the major causes of failure of cancer treatment. This pap...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008060720608
更新日期:1998-09-01 00:00:00