Abstract：
:Explant cultures of porcine coronary artery provided a coculture model, used as a paradigm of arterial wall in contact with vascular prosthesis which allowed the study of spatial and temporal changes in cell phenotype. First cells emerging from the explant had an endothelial phenotype monitored by cytoimmunostaining. Percentages of anti-smooth muscle alpha-actin labelled cells were assessed at early and late phase by flow cytofluorometric analysis to control the effect of heparin. At 100 mug ml(-1), no effect on alpha-actin labelled cell growth has been detected. This result contrasted with the inhibition of monolayer cell cultures. At 500 mug ml(-1), the proliferation of smooth muscle cells was reduced. This explant system should be useful for testing drugs susceptible to interfere with restenosis.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Dufresne M,Warocquier-Clérout Rdoi
10.1023/A:1016195029359subject
Has Abstractpub_date
2001-09-01 00:00:00pages
13-22issue
1eissn
0920-9069issn
1573-0778journal_volume
37pub_type
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