Differentiation of Human Bone Marrow Mesenchymal Stem Cells to Chondrocytes for Construction of Three-dimensional Cartilage Tissue.


:A differentiation method of human bone marrow mesenchymal stem cells (MSCs) to chondrocytes was developed for the construction of a three-dimensional (3D) cartilage tissue. The adhesive cells, which were isolated from a human bone marrow aspirate were embedded in type I collagen in a poly-L: -lactate-glycolic acid copolymer (PLGA) mesh and cultivated for 4 week together with growth factors. The degree of cellular differentiation was estimated by quantitative RT-PCR of aggrecan and type II collagen mRNAs and by staining with Safranin O. The 3D culture showed a higher degree of differentiation even without growth factors than the conventional pellet culture with growth factors, namely, dexamethasone and transforming growth factor (TGF)-beta 3. The 3D culture for 2 week with the combined addition of dexamethasone, TGF-beta 3, and insulin-like growth factor (IGF)-I reached a 30% expression of aggrecan mRNA compared with that in primary human chondrocytes, while the aggrecan mRNA expression in the conventional pellet culture was less than 2%. The sequential two-step differentiation cultivation, during which the cells were cultivated in 3D for 1 week after the conventional two-dimensional (2D) culture for 1 week, could markedly accelerate the expression of aggrecan mRNA compared with the 3D cultivation for 2 week.






Matsuda C,Takagi M,Hattori T,Wakitani S,Yoshida T




Has Abstract


2005-01-01 00:00:00












  • Mathematical models for multidrug resistance and its reversal.

    abstract::Mathematical models describing drug resistance are briefly reviewed. One model which describes the molecular function of the P-glycoprotein pump in multidrug resistant (MDR) cell lines has been developed and is presented in detail. The pump is modeled as an energy dependent facilitated diffusion process. A partial dif...


    pub_type: 杂志文章,评审


    authors: Michelson S

    更新日期:1993-01-01 00:00:00

  • MiR-206 inhibits epilepsy and seizure-induced brain injury by targeting CCL2.

    abstract::To determine the function of miR-206 in epilepsy. Epileptic rat model was established by intra-amygdala injection of kainic acid (KA). Expression levels of miR-206, C-C Motif Chemokine Ligand 2 (CCL2) and interleukin-1β (Il-1β) in hippocampus tissues was measured by reverse transcription-quantitative PCR (RT-qPCR) and...


    pub_type: 杂志文章


    authors: Wu Z,Liu Y,Huang J,Huang Y,Fan L

    更新日期:2019-06-26 00:00:00

  • Improvement in the antibody productivity of human-human hybridomas by transfection with Tac gene.

    abstract::The presence of a highly purified recombinant interleukin-2 (rIL-2) increased the production of immunoglobulin (IgM or IgG) by human-human hybridomas to 1.5-2.0 times the production by untreated cells. However, these cells did not react with anti-Tac (IL-2 receptor alpha) antibody. To enhance the response of the hybri...


    pub_type: 杂志文章


    authors: Iwamoto K,Shintani Y,Sasada R,Honjo T,Kitano K

    更新日期:1990-07-01 00:00:00

  • Anticancer effect of Tamarix gallica extracts on human colon cancer cells involves Erk1/2 and p38 action on G2/M cell cycle arrest.

    abstract::Taking into account that oxidative stress is among the factors causing cancer-related death; chemoprevention which consists in using antioxidant substances such as phenolics could prevent cancer formation and progression. In the present study, phenolic contents and antioxidant activities of methanolic extracts from th...


    pub_type: 杂志文章


    authors: Boulaaba M,Tsolmon S,Ksouri R,Han J,Kawada K,Smaoui A,Abdelly C,Isoda H

    更新日期:2013-12-01 00:00:00

  • Antibodies in the study of multiple drug resistance.

    abstract::Multidrug resistance (MDR) is a major problem in cancer chemotherapy. As P-glycoprotein is the key molecule in MDR, many investigators have constructed anti-P-glycoprotein monoclonal antibodies (MAbs). Those antibodies, including MRK16 and C219, were used for elucidation of the mechanism of MDR and for overcoming of M...


    pub_type: 杂志文章,评审


    authors: Heike Y,Tsuruo T

    更新日期:1993-01-01 00:00:00

  • A methoxyflavanone derivative from the Asian medicinal herb (Perilla frutescens) induces p53-mediated G2/M cell cycle arrest and apoptosis in A549 human lung adenocarcinoma.

    abstract::Perilla frutescens is an Asian dietary herb consumed as an essential seasoning in Japanese cuisine as well as used for a Chinese medicine. Here, we report that a newly found methoxyflavanone derivative from P. frutescens (Perilla-derived methoxyflavanone, PDMF; 8-hydroxy-5,7-dimethoxyflavanone) shows carcinostatic act...


    pub_type: 杂志文章


    authors: Abd El-Hafeez AA,Fujimura T,Kamei R,Hirakawa N,Baba K,Ono K,Kawamoto S

    更新日期:2018-06-01 00:00:00

  • Purification and characterization of lymphocytic clonal growth factor (LCGF) derived from human-human hybridoma SH-76 cells.

    abstract::Human-human hybridoma SH-76 cells were found to produce a factor that supported the growth of lymphocytic cells at low densities. The factor was purified from serum-free conditioned medium of the hybridoma cells by a successive application of ammonium sulfate precipitation, DEAE-Toyopearl, TSK G3000 SW and DEAE-5PW co...


    pub_type: 杂志文章


    authors: Miyata M,Murakami H,Hashizume S,Yamada K,Omura H

    更新日期:1988-11-01 00:00:00

  • Modeling of cell culture processes.

    abstract::Models of cell processes can be particularly useful in simulating, optimizing and controlling cell culture systems. Models reported in the literature are of various degrees of biological structure and mathematical complexity and describe cell growth, death, metabolism, and product formation, alone or in combination wi...


    pub_type: 杂志文章,评审


    authors: Tziampazis E,Sambanis A

    更新日期:1994-01-01 00:00:00

  • Chromatid segregation analysis in native human lymphocyte anaphases using sequential fluorescence in situ hybridization.

    abstract::A sequential multiprobe fluorescence in situ hybridization technique was developed to study the 13, 18, 21, X and Y chromatid segregation in human lymphocytes anaphases cultures without antimitotic treatment. This method was used to know if exist any different chromosomes segregation in lymphocytes from Down syndrome ...


    pub_type: 杂志文章


    authors: Escudero T,Coll MD,Fuster C,Egozcue J

    更新日期:2002-09-01 00:00:00

  • Metabolic rates of vascular endothelial cellsin vitro.

    abstract::Cultures of endothelial cells and cell lines of endothelial origin were maintained at confluence without medium exchange for a period of 72 h. During this time period the concentration of nutrients - amino acids and glucose - and metabolic waste products - lactate and ammonium - was determined as well as cell vitality...


    pub_type: 杂志文章


    authors: Schrimpf G,Schröder M,Weitnauer E,Friedl P

    更新日期:1994-01-01 00:00:00

  • Molecular analysis of cross-reactive human monoclonal antibody AE6F4 generated by in vitro immunization: Epitope mapping of AE6F4 antibody on 14-3-3 family proteins and cytokeratin 8.

    abstract::We reported previously that adenocarcinoma-reactive human monoclonal antibody AE6F4, which had been generated by in vitro immunization method, recognizes both 14-3-3protein and cytokeratin 8 (CK8). In this study, to analyze the cross-reactivity of AE6F4 antibody, epitopes of AE6F4 antibody on 14-3-3 proteins and CK8 w...


    pub_type: 杂志文章


    authors: Ichikawa A,Shim SY,Katakura Y,Teruya K,Hashizume S,Shirahata S

    更新日期:2001-07-01 00:00:00

  • Isolation, culture and identification of pulmonary arterial smooth muscle cells from rat distal pulmonary arteries.

    abstract::The culture of pulmonary arterial smooth muscle cells (PASMCs) is one of the most powerful tools for exploring the mechanisms of pulmonary hypertension (PH). Both pulmonary vasoconstriction and remodeling occur predominantly in distal pulmonary arteries (PA). In this study, we provide our detailed and standardized pro...


    pub_type: 杂志文章


    authors: Peng G,Xu J,Liu R,Fu Z,Li S,Hong W,Chen J,Li B,Ran P

    更新日期:2017-10-01 00:00:00

  • Intracellular localisation studies of doxorubicin and Victoria Blue BO in EMT6-S and EMT6-R cells using confocal microscopy.

    abstract::The subcellular localisation of doxorubicin and Victoria Blue BO (VBBO) in a murine mammary tumour cell line EMT6-S, and the resistant sub-lineEMT6-R was studied, using confocal microscopy, in order to investigate their sites of action. In cells treated with doxorubicin (10 mu M) for 90 min, the pattern of intracellul...


    pub_type: 杂志文章


    authors: Burrow SM,Phoenix DA,Wainwright M,Tobin MJ

    更新日期:2002-05-01 00:00:00

  • Analysis of toxicity effects of Di-(2-ethylhexyl) phthalate exposure on human bronchial epithelial 16HBE cells.

    abstract::Recent studies have indicated that Di-(2-ethylhexyl) phthalate (DEHP), the most commonly used plasticizer in daily-life products, could be dispersed in indoor air and induce human exposure via inhalation. DEHP has been reported to have effects on the respiratory system in both animal and human researches. The toxicity...


    pub_type: 杂志文章


    authors: Ma Y,Guo Y,Wu S,Lv Z,Zhang Q,Xie X,Ke Y

    更新日期:2018-02-01 00:00:00

  • Phytochemical study and evaluation of cytotoxicity, mutagenicity, cell cycle kinetics and gene expression of Bauhinia holophylla (Bong.) Steud. in HepG2 cells in vitro.

    abstract::Bauhinia holophylla (Bong.) Steud. (Fabaceae) is a plant used in Brazilian folk medicine to treat diabetes and inflammation. This study evaluated the phytochemical properties, cytotoxic, apoptotic, mutagenic/antimutagenic effects and alterations in gene expression (RNAm) in HepG2 cells treated with the B. holophylla e...


    pub_type: 杂志文章


    authors: Ribeiro DL,Cilião HL,Specian AFL,Serpeloni JM,De Oliveira MT,Varanda EA,Vilegas W,Saldanha LL,Martínez-López W,Dokkedal AL,Cólus IMS

    更新日期:2018-04-01 00:00:00

  • The role of Sox9 in maintaining the characteristics and pluripotency of Arbas Cashmere goat hair follicle stem cells.

    abstract::In our previous work, we isolated Arbas Cashmere goat hair follicle stem cells (gHFSCs) and explored the pluripotency. In this study, we investigated the expression and putative role of Sox9 in the gHFSCs. Immunofluorescence staining showed that Sox9 is predominantly expressed in the bulge region of the Arbas Cashmere...


    pub_type: 杂志文章


    authors: He N,Dong Z,Tai D,Liang H,Guo X,Cang M,Liu D

    更新日期:2018-08-01 00:00:00

  • Evaluation of the antiviral effect of new compounds by using cellular cultures.

    abstract::Replication Kinetics of HIV was studied under single-growth cycle conditions in C8166 lymphoblastoid cells by back-titrating released and cell-associated infectious virus et each time point. Under these conditions HIV seemed to replicate faster than previously estimated. The amount cell-associated virus always exceede...


    pub_type: 杂志文章


    authors: Dianzani F,Antonelli G

    更新日期:1991-02-01 00:00:00

  • Morphological observation and analysis using automated image cytometry for the comparison of trypan blue and fluorescence-based viability detection method.

    abstract::The ability to accurately determine cell viability is essential to performing a well-controlled biological experiment. Typical experiments range from standard cell culturing to advanced cell-based assays that may require cell viability measurement for downstream experiments. The traditional cell viability measurement ...


    pub_type: 杂志文章


    authors: Chan LL,Kuksin D,Laverty DJ,Saldi S,Qiu J

    更新日期:2015-05-01 00:00:00

  • Effect of Partial Medium Replacement on Cell Growth and Protein Production for the High-Fivetrade mark insect cell line.

    abstract::The potential of spent medium to support the growth and recombinant protein production of High-Fivetrade mark cells was investigated. Growth in medium consisting of three parts fresh and one part spent medium was comparable to that in fresh medium (maximal specific growth rates of 0.028 and 0.029 h(-1), and maximal ce...


    pub_type: 杂志文章


    authors: Ikonomou L,Bastin G,Schneider YJ,Agathos SN

    更新日期:2004-01-01 00:00:00

  • Insect cells respiratory activity in bioreactor.

    abstract::Specific respiration rate ( [Formula: see text]) is a key parameter to understand cell metabolism and physiological state, providing useful information for process supervision and control. In this work, we cultivated different insect cells in a very controlled environment, being able to measure [Formula: see text]. Sp...


    pub_type: 杂志文章


    authors: Pamboukian MM,Jorge SA,Santos MG,Yokomizo AY,Pereira CA,Tonso A

    更新日期:2008-05-01 00:00:00

  • Visible light regulates neurite outgrowth of nerve cells.

    abstract::The neurite outgrowth of PC12 cells on collagen-coated glass plates under light emitting diode (LED) irradiation at several wavelengths (i.e., 455, 470, 525, 600, 630, 880 and 945 nm) was investigated. No neurite outgrowth was observed during cultivation under irradiation from the lamp of an inverted light microscope ...


    pub_type: 杂志文章


    authors: Higuchi A,Watanabe T,Noguchi Y,Chang Y,Chen WY,Matsuoka Y

    更新日期:2007-07-01 00:00:00

  • Localization of viral transforming sequences within marker chromosomes associated with tumor formation and progression in a murine fibrosarcoma.

    abstract::The low-metastatic RSV-transformed fibrosarcoma line B77-3T3 and its metastatic variant AA12, selected in vitro, have been analysed by blot and in situ hybridization with v-src and murine c-myc specific probes in order to detect molecular rearrangements underlying the transition from the low-metastatic to the high-met...


    pub_type: 杂志文章


    authors: Doneda L,Custode P,Morghen Cde G,Larizza L

    更新日期:1987-10-01 00:00:00

  • Generation of lymphokine activated killer cells in a new high density dialyzing culture apparatus.

    abstract::In order to establish an efficient culture system for the generation of lymphokine activated killer (LAK) cells, we have developed a new device which is essentially based on a continuous dialyzing culture vessel. LAK cells grown in such a system showed higher cytotoxicity than those grown under conventional culture co...


    pub_type: 杂志文章


    authors: Kohgo Y,Sakamaki S,Kanisawa Y,Nojiri S,Ueno Y,Itoh Y,Takahashi M,Sasagawa Y,Hosoi S,Sato S,Niitsu Y

    更新日期:1989-02-01 00:00:00

  • Cyanidin-3-glucoside enhances mitochondrial function and biogenesis in a human hepatocyte cell line.

    abstract::Mitochondrial dysfunction has been identified as one of the primary factors contributing to liver diseases. Pathways that control mitochondrial biogenesis are potential therapeutic targets for the amelioration of hepatocyte dysfunction and liver disease. Research on natural pharmacological agents that ameliorate liver...


    pub_type: 杂志文章


    authors: Mogalli R,Matsukawa T,Shimomura O,Isoda H,Ohkohchi N

    更新日期:2018-12-01 00:00:00

  • Comparison of RAP-PCR analysis of gene expression in fresh and immortalised rat hepatocyte cell lines.

    abstract::Primary rat hepatocytes dedifferentiate rapidly losing theactivities of the drug metabolising enzymes involved in thedetoxification of xenobiotics in the liver. An alternativeapproach to using primary hepatocytes for toxicity testing isthe development of immortalised hepatocyte cell lines via thetransfection of primar...


    pub_type: 杂志文章


    authors: Kelly HT,Hill E,Reid F,Grant MH,Macdonald C

    更新日期:2000-10-01 00:00:00

  • Relative survivability of human osteoblasts is enhanced by 39 °C and ascorbic acid after exposure to photopolymerization ingredients.

    abstract::Photopolymerizable hydrogels offer great potential in cartilage tissue engineering due to their ability to conform to irregular defect shapes and be applied in a potentially minimally invasive manner. An important process requirement in the use of photopolymerizable hydrogels is the ability of the suspended cells to w...


    pub_type: 杂志文章


    authors: Dua R,Ramaswamy S

    更新日期:2013-08-01 00:00:00

  • Inhibition of hepatoma cell invasion beneath mesothelial-cell monolayer by sera from tea- and related component-treated rats and their modes of action.

    abstract::The bioavailability and action of teas on the invasion of a rat ascites hepatoma cell line, AH109A, were determined and their modes of action were by co-culturing the cancer cells with a rat mesentery-derived mesothelial-cell (M-cell) monolayer in the presence of sera from rats orally given teas and their component, (...


    pub_type: 杂志文章


    authors: Zhang G,Miura Y,Yagasaki K

    更新日期:2001-07-01 00:00:00

  • Enhanced secretory ability for the human factor VIII light chain produced in baculovirus-infected insect cells.

    abstract::The light and truncated heavy chains of human factor VIII, expressed separately in baculovirus-infected insect cells, exhibited different secretory behaviour when compared with each other and with a biologically active fusion molecule of the truncated heavy and light chains. The light chain was very efficiently secret...


    pub_type: 杂志文章


    authors: Webb E,Tkalcevic J,Edwards S,Hocking D,Nisbet I

    更新日期:1996-01-01 00:00:00

  • Highly passage of Spodoptera litura cell line causes its permissiveness to baculovirus infection.

    abstract::It is well known that the characteristics of cell lines possibly alter when cell lines are at high-passage number because of the environmental selection. We do not know whether non-permissive or low-permissive cell lines could become permissive or more permissive to virus infection after over-high passage. In the pres...


    pub_type: 杂志文章


    authors: Zhang X,Lan W,Deng Y,Ma Y,Liu K,Peng J,Li Y,Hong H

    更新日期:2008-07-01 00:00:00

  • Alteration of mammalian cell metabolism by dynamic nutrient feeding.

    abstract::The metabolism of hybridoma cells was controlled to reduce metabolic formation in fed-batch cultures by dynamically feeding a salt-free nutrient concentrate. For this purpose, on-line oxygen uptake rate (OUR) measurement was used to estimate the metabolic demand of hybridoma cells and to determine the feeding rate of ...


    pub_type: 杂志文章


    authors: Zhou W,Rehm J,Europa A,Hu WS

    更新日期:1997-07-01 00:00:00