Insect cells respiratory activity in bioreactor.

abstract：:Fibroblast growth factor 21 (FGF21) potentially regulates glucose and lipid metabolism in energy homeostasis. We investigated dynamic changes in goat adipocytes treated with 75 nM FGF21 for 24, 36 and 48 h. Compared to controls, FGF21-treated adipocytes displayed smaller lipid droplets and altered levels of the mRNA t...

journal_title：Cytotechnology

authors： Zhang Y,Li L,Wang Q,Zhan S,Wang L,Zhong T,Guo J,Zhang H

更新日期：2018-10-01 00:00:00

abstract：:More than 35 years ago double Nobel laureate Linus Carl Pauling published a powerful model of the molecular mechanism of general anesthesia, generally referred to as the hydrate-microcrystal (aqueous-phase) theory. This hypothesis, based on the molecular behavior of water molecules, did not receive serious attention d...

journal_title：Cytotechnology

authors： Nakada T

更新日期：2009-04-01 00:00:00

abstract：:The cytotoxic necrotizing factor type 1 (CNF1) fromE. coli causes necrosis in rabbit skin and multinucleation in cultured cells. Cells exposed to CNF1 were characterized by changes in actin organization, mainly consisting in the presence of well-developed stress fibers and membrane ruffles. The interaction of CNF1 wit...

journal_title：Cytotechnology

authors： Falzano L,Fiorentini C,Boquet P,Donelli G

更新日期：1993-01-01 00:00:00

abstract：:The efficacy of hepatocellular carcinoma (HCC) treatment is very low because of the high percentage of recurrence and resistance to anticancer agents. Hepatic cancer stem cells (HCSCs) are considered the origin of such recurrence and resistance. Our aim was to evaluate the stemness of doxorubicin and 5-fluorouracil re...

journal_title：Cytotechnology

authors： Vu NB,Nguyen TT,Tran LC,Do CD,Nguyen BH,Phan NK,Pham PV

更新日期：2013-08-01 00:00:00

abstract：:In recent years, water pollution has been converted to a challenging discussion in health area of human being. Heavy elements are one of the most important water pollutants and their negative adverse effects on body systems have been confirmed. In this study, investigation of effects of two heavy elements including le...

journal_title：Cytotechnology

authors： Radbin R,Vahedi F,Chamani J

更新日期：2014-10-01 00:00:00

abstract：:In construction of epithelial cells as multilayers, the cells are grown submerged to confluence on fibroblast-embedded collagen gels and, then, lifted to air to promote their stratification. We recently demonstrated that gingival epithelial cells form uniform monolayers on semi-permeable nitrocellulose membranes, supp...

journal_title：Cytotechnology

authors： Gursoy UK,Gursoy M,Könönen E,Sintim HO,Uitto VJ,Syrjänen S

更新日期：2016-12-01 00:00:00

abstract：:Disabled Infectious Single Cycle (DISC) HSV-2 has been cultured in the complimentary cell line CR2 to provide high titre bulk material suitable for the purification of the virus as a live viral vaccine. CR2 cells are cultured on the microcarrier Cytodex-1 at 5 g l-1 in small scale (1 l) and larger scale (15 l) reactor...

journal_title：Cytotechnology

authors： Zecchini TA,Smith RJ

更新日期：1999-07-01 00:00:00

abstract：:The Pseudopimelodidae family comprises 35 species however, cytogenetic studies have been performed in only six species. This study uncovered karyotypic data on Pseudopimelodus pulcher and Microglanis cottoides. Both species possessed 2n = 54, with 20m + 16sm + 10st + 8a and FN = 100 for P. pulcher and 30m + 14sm + 6st...

journal_title：Cytotechnology

authors： Gouveia JG,de Moraes VP,Pires LB,da Rosa R,Dias AL

更新日期：2015-03-01 00:00:00

abstract：:Celecoxib is a selective cyclooxygenase-2 (COX-2) inhibitor with antitumor and antiangiogenic activities. To investigate the effects of celecoxib on nasopharyngeal carcinoma (NPC), HNE-1 cells were treated with celecoxib at various concentrations. MTT assay, migration assay and invasion assay were performed to observe...

journal_title：Cytotechnology

authors： Chen J,Ran Y,Hong C,Chen Z,You Y

更新日期：2010-10-01 00:00:00

abstract：:ADG cell line was established from an abalonedigestive gland and previously characterized. ADGcells have the potential to grow in protein-freeculture and secrete l3 types of glycosidases. Inthis article, we determined the origin of ADG cell line,using electron microscopy, and purified a glycosidasesecreted by these ce...

journal_title：Cytotechnology

authors： Kusumoto K,Shirahata S,Kamei Y

更新日期：2000-07-01 00:00:00

abstract：:Lentiviruses are quite effective gene delivery systems for stable production of genetically engineered human cells. However, prior to using lentivirus to deliver genetic materials to cells of interest, the normal course of production of these lentiviruses involves a lengthy collection, purification, preservation, and ...

journal_title：Cytotechnology

authors： Timmins LM,Patel RS,Teryek MS,Parekkadan B

更新日期：2019-10-01 00:00:00

abstract：:2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a persistent and ubiquitous environmental contaminant. The health impact of TCDD exposure is of great concern to the general public. Recent reports have implied that eicosapentaenoic acid (EPA) might be a potential chemopreventive agent and influence hepatotoxicity. The ai...

journal_title：Cytotechnology

authors： Turkez H,Geyikoglu F,Mokhtar YI,Togar B

更新日期：2012-01-01 00:00:00

abstract：:Iron overload is a major health problem for patients who have to have continuous blood transfusions. It brings some metabolic problems together. Various iron chelating agents are being used for treatment of hemochromatosis which arises from excess iron accumulation. This study was conducted with the aim of determining...

journal_title：Cytotechnology

authors： Ila HB,Topaktas M,Arslan M,Büyükleyla M

更新日期：2014-08-01 00:00:00

abstract：:We describe a novel fully automated high-throughput time-lapse microscopy system and evaluate its performance for precisely tracking the motility of several glioma and osteoblastic cell lines. Use of this system revealed cell motility behavior not discernable with conventional techniques by collecting data (1) from cl...

journal_title：Cytotechnology

authors： Fotos JS,Patel VP,Karin NJ,Temburni MK,Koh JT,Galileo DS

更新日期：2006-05-01 00:00:00

abstract：:Dental pulp stem cells (DPSCs), one type of mesenchymal stem cells, are considered to be a type of tool cells for regenerative medicine and tissue engineering. Our previous studies found that the stimulation with lipopolysaccharide (LPS) might introduce senescence of DPSCs, and this senescence would have a positive co...

journal_title：Cytotechnology

authors： Feng G,Zheng K,Cao T,Zhang J,Lian M,Huang D,Wei C,Gu Z,Feng X

更新日期：2018-06-01 00:00:00

abstract：:The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rb1 was produced by fusing splenocytes immunized with a ginsenoside Rb1- bovine serum albumin conjugate wit...

journal_title：Cytotechnology

authors： Tanaka H,Fukuda N,Shoyama Y

更新日期：1999-03-01 00:00:00

abstract：:Mesenchymal stromal cells (MSCs) are promising tools in regenerative medicine and targeted therapies. Although different origins have been described, there is still huge need to find a valuable source harboring specific subpopulations of MSCs with precise therapeutic functions. Here, we isolated by fluorescence activa...

journal_title：Cytotechnology

authors： Najar M,Crompot E,van Grunsven LA,Dollé L,Lagneaux L

更新日期：2019-02-01 00:00:00

abstract：:By employing the affinity gel fraction technique, we have detected a 175 kDa tyrosine-O-sulfate (TyrS)-binding protein in sodium choleate extracts of the microsomal membrane fractions of bovine liver and pancreas, as well as canine liver and pancreas. Western blot analysis revealed the presence of the bovine liver Tyr...

journal_title：Cytotechnology

authors： Liu MC,Sakakibara Y,Suiko M

更新日期：1997-01-01 00:00:00

abstract：:A silicon microsensor (ISFET - Ion Sensitive Field Effect Transistor) has been used to detect the metabolism of a cell population cultured on a coverslip and positioned close to the sensor surface. The system output is analyzed as a function of cell density. ...

journal_title：Cytotechnology

authors： Martinoia S,Meloni M,Parodi MT,Tedesco M,Ciccarelli C,Grattarola M

更新日期：1993-01-01 00:00:00

abstract：:Human herpes virus 6 (HHV-6) is a common human pathogen that is most often detected in hematopoietic cells. Although human cells harboring chromosomally integrated HHV-6 can be generated in vitro, the availability of such cell lines originating from in vivo tissues is limited. In this study, chromosomally integrated H...

journal_title：Cytotechnology

authors： Shioda S,Kasai F,Ozawa M,Hirayama N,Satoh M,Kameoka Y,Watanabe K,Shimizu N,Tang H,Mori Y,Kohara A

更新日期：2018-02-01 00:00:00

abstract：:Low-frequency dielectric spectroscopy has been used in situ, i.e. while the cells are still attached to their microsupport, to monitor the changes of biomass accompanying the growth of anchorage-dependent cells. This method, when compared to Aperture Impedance Pulse Spectroscopy (also called electronic sizing), is cha...

journal_title：Cytotechnology

authors： Degouys V,Cerckel I,Garcia A,Harfield J,Dubois D,Fabry L,Miller AO

更新日期：1993-01-01 00:00:00

abstract：:The degranulation of basophils stimulated by antigen is one of the allergic mechanisms of immediate hypersensitivity reactions. In a previous study, the β-hexosaminidase release of rat basophilic leukemia (RBL-2H3) cells stimulated by IgE cross-linking was specifically inhibited by ethanol extract of olive milled wast...

journal_title：Cytotechnology

authors： Kishikawa A,Amen Y,Shimizu K

更新日期：2017-04-01 00:00:00

abstract：:A Tubular Liquid Film Reactor was designed as a model system to transfer a batch culture kinetic to a continuous cascade. Cell density, product formation and substrate consumption rates were followed during fermentation at two dilution rates. In spite of the high dilution rates effective in each segment by itself high...

journal_title：Cytotechnology

authors： Borth N,Steindl F,Weigang F,Reiter M,Katinger H

更新日期：1990-05-01 00:00:00

abstract：:Successful culturing of neurons from adult animals has been historically difficult for a relatively long time. In this study, we reported the development of a novel method for the isolation and the culture of major pelvic ganglion (MPG) neurons from adult rat. The cultured cells were identified by neuron morphology an...

journal_title：Cytotechnology

authors： Cheng S,Yang X,Zhang Y,Xiao C

更新日期：2013-08-01 00:00:00

abstract：:Menadione-catalyzed H(2)O(2) production by viable animal cells was proportional to the viable cell number, and H(2)O(2) production decreased with increasing cytotoxic effects after the incubation of cells with cytotoxic compounds. The cytotoxic effects of food additives, pesticides, antibiotics, heavy metals, phytotox...

journal_title：Cytotechnology

authors： Yamashoji S,Isshiki K

更新日期：2001-11-01 00:00:00

abstract：:In previous studies, vascular smooth muscle progenitor cells (vSMPCs) isolated from peripheral blood mononuclear cells (PBMCs) were cultured using medium containing platelet-derived growth factor-BB (PDGF-BB) for 4 weeks. However, this method requires long culture periods of up to 4 weeks and yields low cell counts. T...

journal_title：Cytotechnology

authors： Seong JH,Song YS,Joo HW,Park IH,Shen GY,Shin NK,Lee AH,Kwon AM,Lee Y,Kim H,Kim KS

更新日期：2020-10-01 00:00:00

abstract：:We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum free media and suspension growth. Subsequent...

journal_title：Cytotechnology

authors： Schoofs G,Monica TJ,Ayala J,Horwitz J,Montgomery T,Roth G,Castillo FJ

更新日期：1998-11-01 00:00:00

abstract：:Although the insect cell/baculovirus system is an important expression platform for recombinant protein production, our understanding of insect cell metabolism with respect to enhancing cell growth capability and productivity is still limited. Moreover, different host insect cell lines may have different growth charac...

journal_title：Cytotechnology

authors： Chou CC

更新日期：2014-10-01 00:00:00

abstract：:Bombyx batryticatus is a traditional Chinese medicine. To understand apoptotic effect of B. batryticatus ethanol extract (BBE), we investigated the role of BBE in inducing apoptosis of human gastric cancer cells SGC-7901. Cells treated with BBE and apoptosis was assessed by methyl thiazolyl tetrazolium (MTT) assay, mo...

journal_title：Cytotechnology

authors： Wu JY,Sheikho A,Ma H,Li TC,Zhao YQ,Zhang YL,Wang D

更新日期：2017-12-01 00:00:00

abstract：:A hallmark of human cancer cells is immortal cell growth, which is associated with telomere maintenance by telomerase. The transcriptional regulation of the human telomerase reverse transcriptase (hTERT) gene is a major mechanism that negatively and positively controls telomerase activity in normal and cancer cells, r...

journal_title：Cytotechnology

authors： Horikawa I,Michishita E,Barrett JC

更新日期：2004-06-01 00:00:00