A continuous multistage roller reactor for animal cell culture: 1. Patterns of growth, production and catabolism of a murine hybridoma.

Abstract:

:A Tubular Liquid Film Reactor was designed as a model system to transfer a batch culture kinetic to a continuous cascade. Cell density, product formation and substrate consumption rates were followed during fermentation at two dilution rates. In spite of the high dilution rates effective in each segment by itself high cell densities of up to 10(7) cells/ml were achieved due to cell sedimentation. The model character of the reactor was taken to determine critical values of substrate concentrations that influence production rates and result in an adaptation of metabolism.

journal_name

Cytotechnology

journal_title

Cytotechnology

authors

Borth N,Steindl F,Weigang F,Reiter M,Katinger H

doi

10.1007/BF00365489

subject

Has Abstract

pub_date

1990-05-01 00:00:00

pages

253-8

issue

3

eissn

0920-9069

issn

1573-0778

journal_volume

3

pub_type

杂志文章
  • The enhancement of growth and differentiation of rat adrenal nerve cells by the addition of conditioned medium from human fibroblast cultures.

    abstract::The growth of rat adrenal nerve cells was remarkably enhanced by supplementing the cultured medium from the human fibroblast cell line, Hs 68. Maximum specific growth rate and length of the neurites were observed as 0.076 (1/hr) and 0.026 mm, respectively in 20% supplement of five day old medium. In adding more than 2...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1023/A:1007942619463

    authors: Hong JS,Kim DS,Kim SH,Choi DH,Lee JH,Lee HY

    更新日期:1998-03-01 00:00:00

  • Relative survivability of human osteoblasts is enhanced by 39 °C and ascorbic acid after exposure to photopolymerization ingredients.

    abstract::Photopolymerizable hydrogels offer great potential in cartilage tissue engineering due to their ability to conform to irregular defect shapes and be applied in a potentially minimally invasive manner. An important process requirement in the use of photopolymerizable hydrogels is the ability of the suspended cells to w...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-012-9512-8

    authors: Dua R,Ramaswamy S

    更新日期:2013-08-01 00:00:00

  • Isolation, culture and identification of pulmonary arterial smooth muscle cells from rat distal pulmonary arteries.

    abstract::The culture of pulmonary arterial smooth muscle cells (PASMCs) is one of the most powerful tools for exploring the mechanisms of pulmonary hypertension (PH). Both pulmonary vasoconstriction and remodeling occur predominantly in distal pulmonary arteries (PA). In this study, we provide our detailed and standardized pro...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-017-0081-8

    authors: Peng G,Xu J,Liu R,Fu Z,Li S,Hong W,Chen J,Li B,Ran P

    更新日期:2017-10-01 00:00:00

  • Primary cell culture from embryos of the Japanese scallop Mizuchopecten yessoensis (Bivalvia).

    abstract::Primary cell cultures obtained from embryos of Mizuchopecten yessoensis (Bivalvia) survived for four months. Although the number of cells progressively decreased during the cultivation, mitotic cells were observed both at the first stages and at the end. A possibility of growing marine invertebrates cells in long term...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00353702

    authors: Odintsova NA,Khomenko AV

    更新日期:1991-05-01 00:00:00

  • Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells.

    abstract::The adult heart contains a population of cardiac progenitor cells (CPCs). Growing and collecting an adequate number of CPCs demands complex culture media containing growth factors. Since activated macrophages secrete many growth factors, we investigated if activated isolated heart cells seeded on a feeder layer of act...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-014-9814-0

    authors: Sepúlveda DE,Cabeza Meckert P,Locatelli P,Olea FD,Pérez NG,Pinilla OA,Díaz RG,Crottogini A,Laguens RP

    更新日期:2016-08-01 00:00:00

  • Anti-proliferative and apoptotic effects of oleuropein and hydroxytyrosol on human breast cancer MCF-7 cells.

    abstract::Olive oil intake has been shown to induce significant levels of apoptosis in various cancer cells. These anti-cancer properties are thought to be mediated by phenolic compounds present in olive. These beneficial health effects of olive have been attributed, at least in part, to the presence of oleuropein and hydroxyty...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-009-9191-2

    authors: Han J,Talorete TP,Yamada P,Isoda H

    更新日期:2009-01-01 00:00:00

  • Early detection of cell metabolism with a silicon microsensor.

    abstract::A silicon microsensor (ISFET - Ion Sensitive Field Effect Transistor) has been used to detect the metabolism of a cell population cultured on a coverslip and positioned close to the sensor surface. The system output is analyzed as a function of cell density. ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00746064

    authors: Martinoia S,Meloni M,Parodi MT,Tedesco M,Ciccarelli C,Grattarola M

    更新日期:1993-01-01 00:00:00

  • Synthesis, characterization and cytotoxicity of boron nitride nanoparticles: emphasis on toxicogenomics.

    abstract::Nanotechnology is increasingly developing area including more than 700 commercial products such as clothing, food preparation, cosmetics, mechanics, electronics and also health industry. People generally contact with nanoparticles by inhaling from air. Thus, it is becoming important issue to understand harmful effects...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-019-00292-8

    authors: Türkez H,Arslan ME,Sönmez E,Açikyildiz M,Tatar A,Geyikoğlu F

    更新日期:2019-02-01 00:00:00

  • Differentiation of Human Bone Marrow Mesenchymal Stem Cells to Chondrocytes for Construction of Three-dimensional Cartilage Tissue.

    abstract::A differentiation method of human bone marrow mesenchymal stem cells (MSCs) to chondrocytes was developed for the construction of a three-dimensional (3D) cartilage tissue. The adhesive cells, which were isolated from a human bone marrow aspirate were embedded in type I collagen in a poly-L: -lactate-glycolic acid cop...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-005-3751-x

    authors: Matsuda C,Takagi M,Hattori T,Wakitani S,Yoshida T

    更新日期:2005-01-01 00:00:00

  • Expression of recombinant Atlantic salmon serum C-type lectin in Drosophila melanogaster Schneider 2 cells.

    abstract::The Atlantic salmon (Salmo salar) serum lectin (SSL) is a soluble C-type lectin that binds bacteria, including salmon pathogens. This lectin is a cysteine-rich oligomeric protein. Consequently, a Drosophila melanogaster expression system was evaluated for use in expressing SSL. A cDNA encoding SSL was cloned into a ve...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-012-9505-7

    authors: Uribe E,Venkatesan M,Rose DR,Ewart KV

    更新日期:2013-08-01 00:00:00

  • Evaluation of monitoring approaches and effects of culture conditions on recombinant protein production in baculovirus-infected insect cells.

    abstract::The baculovirus infection process of Spodoptera frugiperda (Sf9) insect cells in oxygen-controlled bioreactors in serum-free medium was investigated using a recombinant Autographa californica (AcNPV) virus expressing beta-galactosidase enzyme as a model system. A variety of monitoring techniques including trypan blue ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00762392

    authors: Hensler WT,Agathos SN

    更新日期:1994-01-01 00:00:00

  • Adaptation of the Se301 insect cell line to suspension culture. Effect of turbulence on growth and on production of nucleopolyhedrovius (SeMNPV).

    abstract::As chemical pesticides are being banned as control agents for agricultural pests, the use of the highly specific, safe to non-target organisms baculoviruses has been proposed. These viruses can be produced either in vivo or in vitro. In vitro production requires appropriated host insect cell lines with the ability for...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-011-9387-0

    authors: Beas-Catena A,Sánchez-Mirón A,García-Camacho F,Molina-Grima E

    更新日期:2011-12-01 00:00:00

  • Improved reproducibility in preparing precision-cut liver tissue slices.

    abstract::Precision-cut liver tissue slices (PCLS) have been used for decades to study pharmacological metabolism as well as toxicology and efficacy of novel substances on primary material under standardized conditions. Slicing of primary liver tissue has been done using different slicing machines. Since there has been great va...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-009-9246-4

    authors: Zimmermann M,Lampe J,Lange S,Smirnow I,Königsrainer A,Hann-von-Weyhern C,Fend F,Gregor M,Bitzer M,Lauer UM

    更新日期:2009-12-01 00:00:00

  • Neurite outgrowth promoting activity of marine algae from Japan against rat adrenal medulla pheochromocytoma cell line, PC12D.

    abstract::We screened for biological activity which induces neurite outgrowth in vitro from 300 species of marine algae from along the Japan coast for possible use as a treatment for the lack of neurotrophic factor which is considered to be a cause of Alzheimer's disease. In this study, we evaluated the neurite outgrowth promot...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1023/A:1023974323807

    authors: Kamei Y,Sagara A

    更新日期:2002-11-01 00:00:00

  • MiR-548c-3p inhibits the proliferation, migration and invasion of human breast cancer cell by targeting E2F3.

    abstract::MiR-548 has been reported to be involved in a variety of tumor processes, but its function in breast cancer remains unclear. In this study, we found that miR-548 was low expressed in breast cancer tissues and cells compared with normal control. We then examined whether up-regulation of miR-548 could improve the progre...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-020-00418-3

    authors: Tan PY,Wen LJ,Li HN,Chai SW

    更新日期:2020-10-01 00:00:00

  • Liver stem cells.

    abstract::The capacity of hepatocytes and cholangiocytes to contribute to their own maintenance has long been recognized. More recently, studies have indicated the presence of both intra-hepatic and extra-hepatic stem/progenitor cell populations. The intraorgan compartment probably derives primarily from the biliary tree, most ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1023/A:1024826823194

    authors: Theise ND

    更新日期:2003-03-01 00:00:00

  • Alteration of mammalian cell metabolism by dynamic nutrient feeding.

    abstract::The metabolism of hybridoma cells was controlled to reduce metabolic formation in fed-batch cultures by dynamically feeding a salt-free nutrient concentrate. For this purpose, on-line oxygen uptake rate (OUR) measurement was used to estimate the metabolic demand of hybridoma cells and to determine the feeding rate of ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1023/A:1007945826228

    authors: Zhou W,Rehm J,Europa A,Hu WS

    更新日期:1997-07-01 00:00:00

  • The role of Sox9 in maintaining the characteristics and pluripotency of Arbas Cashmere goat hair follicle stem cells.

    abstract::In our previous work, we isolated Arbas Cashmere goat hair follicle stem cells (gHFSCs) and explored the pluripotency. In this study, we investigated the expression and putative role of Sox9 in the gHFSCs. Immunofluorescence staining showed that Sox9 is predominantly expressed in the bulge region of the Arbas Cashmere...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-018-0206-8

    authors: He N,Dong Z,Tai D,Liang H,Guo X,Cang M,Liu D

    更新日期:2018-08-01 00:00:00

  • Use of cell cultures, nuclear and radioanalytical techniques for metallotoxicological studies at the JRC-Ispra.

    abstract::This paper reviews the JRC-Ispra research activity on toxicological studies related to the environmental and occupational exposure of trace metals, as carried out by a combination of cell culture methodologies with nuclear and radioanalytical techniques. Applications concern the setting of uptake-effect relationships ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:

    authors: Sabbioni E,Pozzi G,Fischbach M,Bahbouth E

    更新日期:1993-01-01 00:00:00

  • Efficient production of recombinant IgG by metabolic control and co-expression with GLUT5 in a fructose-based medium.

    abstract::A fructose-based cell culture is suitable for the process control of protein production because of slow sugar consumption rate and low lactate accumulation. The fructose transporter, GLUT5, mediates its incorporation into cells and is required for the fructose-based culture. In order to produce efficiently recombinant...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-010-9289-6

    authors: Inoue Y,Tsukamoto Y,Yamanaka M,Nakamura S,Inoue A,Nishino N,Kawahara H

    更新日期:2010-08-01 00:00:00

  • A luciferase complementation assay system using transferable mouse artificial chromosomes to monitor protein-protein interactions mediated by G protein-coupled receptors.

    abstract::G protein-coupled receptors (GPCRs) are seven-transmembrane domain receptors that interact with the β-arrestin family, particularly β-arrestin 1 (ARRB1). GPCRs interact with 33% of small molecule drugs. Ligand screening is promising for drug discovery concerning GPCR-related diseases. Luciferase complementation assay ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-018-0231-7

    authors: Uno N,Fujimoto T,Komoto S,Kurosawa G,Sawa M,Suzuki T,Kazuki Y,Oshimura M

    更新日期:2018-12-01 00:00:00

  • Automated time-lapse microscopy and high-resolution tracking of cell migration.

    abstract::We describe a novel fully automated high-throughput time-lapse microscopy system and evaluate its performance for precisely tracking the motility of several glioma and osteoblastic cell lines. Use of this system revealed cell motility behavior not discernable with conventional techniques by collecting data (1) from cl...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-006-9006-7

    authors: Fotos JS,Patel VP,Karin NJ,Temburni MK,Koh JT,Galileo DS

    更新日期:2006-05-01 00:00:00

  • Chromatid segregation analysis in native human lymphocyte anaphases using sequential fluorescence in situ hybridization.

    abstract::A sequential multiprobe fluorescence in situ hybridization technique was developed to study the 13, 18, 21, X and Y chromatid segregation in human lymphocytes anaphases cultures without antimitotic treatment. This method was used to know if exist any different chromosomes segregation in lymphocytes from Down syndrome ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1023/A:1023945218123

    authors: Escudero T,Coll MD,Fuster C,Egozcue J

    更新日期:2002-09-01 00:00:00

  • Human fetal aorta-derived vascular progenitor cells: identification and potential application in ischemic diseases.

    abstract::Vasculogenesis, the formation of blood vessels in embryonic or fetal tissue mediated by immature vascular cells (i.e., angioblasts), is poorly understood. Here we report a summary of our recent studies on the identification of a population of vascular progenitor cells (VPCs) in human fetal aorta. These undifferentiate...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-008-9167-7

    authors: Invernici G,Madeddu P,Emanueli C,Parati EA,Alessandri G

    更新日期:2008-09-01 00:00:00

  • Regulation and expression of transforming growth factor type-beta during early mammalian development.

    abstract::We have examined the effect of differentiation on the expression of different members of the transforming growth factor type-beta (TGF-beta) family using embryonal carcinoma (EC) cells and early mammalian embryos. We determined that TGF-beta activity increases approximately 25-100% when the mouse EC cell line, F9, is ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00563783

    authors: Kelly D,Campbell WJ,Tiesman J,Rizzino A

    更新日期:1990-11-01 00:00:00

  • Features of free software packages in flow cytometry: a comparison between four non-commercial software sources.

    abstract::Flow cytometers designed to analyze large particles are enabling new applications in biology. Data analysis is a critical component of the process FCM. In this article we compare features of four free software packages including WinMDI, Cyflogic, Flowing software, and Cytobank. ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-013-9609-8

    authors: Sahraneshin Samani F,Moore JK,Khosravani P,Ebrahimi M

    更新日期:2014-08-01 00:00:00

  • Antidiabetic effect of green rooibos (Aspalathus linearis) extract in cultured cells and type 2 diabetic model KK-A(y) mice.

    abstract::Previous studies have demonstrated antidiabetic effects for rooibos (Aspalathus linearis) and aspalathin (ASP), one of its main polyphenols. Rooibos, an endemic plant of South Africa, is well-known for its use as herbal tea. Green ('unfermented') rooibos has been shown to contain more ASP than 'fermented' rooibos tea,...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-014-9816-y

    authors: Kamakura R,Son MJ,de Beer D,Joubert E,Miura Y,Yagasaki K

    更新日期:2015-08-01 00:00:00

  • The role of oncostatin M regulates osteoblastic differentiation of dental pulp stem cells through STAT3 pathway.

    abstract::Dental pulp stem cells (DPSCs) are a type of mesenchymal stem cells, which have the self-renewal and multi-lineage differentiation potential, including chondrocytes, adipocytes, neural cells and osteoblasts. So they play a significant role in pulp repair and bone regeneration. Oncostatin M (OSM), one of the IL-6 famil...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-016-9995-9

    authors: Feng X,Shen S,Cao P,Zhu L,Zhang Y,Zheng K,Feng G,Zhang D

    更新日期:2016-12-01 00:00:00

  • The cell yields and biological characteristics of stromal/stem cells from lipoaspirate with different digestion loading ratio.

    abstract::Effective harvesting procedure for adipose tissue is demanded by the affordable Good Manufacturing Practice-Compliant Production of clinical-grade adipose tissue-derived stem cells (hADSCs). Enzymatic digestion using collagenase is the most reliable method of adipose tissue-derived stem cells (hADSCs) isolation, while...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-020-00369-9

    authors: Li Z,Mu D,Liu C,Xin M,Fu S,Li S,Qi J,Wang Q,Luan J

    更新日期:2020-04-01 00:00:00

  • Hypoxia enhances proliferation and stemness of human adipose-derived mesenchymal stem cells.

    abstract::The aim of the study was to obtain the highest number of multipotent adipose-derived mesenchymal stem cells (ADMSCs) by using culture conditions which favour cell expansion without loss of mesenchymal stem cells (MSC)-like properties. Based on the assumption that stem cells reside in niches characterized by hypoxic co...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-014-9731-2

    authors: Fotia C,Massa A,Boriani F,Baldini N,Granchi D

    更新日期:2015-12-01 00:00:00