Abstract:
:The growth of rat adrenal nerve cells was remarkably enhanced by supplementing the cultured medium from the human fibroblast cell line, Hs 68. Maximum specific growth rate and length of the neurites were observed as 0.076 (1/hr) and 0.026 mm, respectively in 20% supplement of five day old medium. In adding more than 20% of the cultured medium both cell and neurite growth was severely decreased. It was interesting that the cultured medium from Hs 68 cells could play a role in the extension of the neurites rather than in the growth of neurite cells. It was also found that molecules lower than 50,000 daltons in the conditioned medium could improve the growth of neurite bearing cells and the extension of the neurites than larger molecules. The efficacy of the proteins (<50,000 MW) was similar to that of human nerve growth factor and much better than that of basic fibroblast growth factor which was mainly secreted from human fibroblast cells.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Hong JS,Kim DS,Kim SH,Choi DH,Lee JH,Lee HYdoi
10.1023/A:1007942619463subject
Has Abstractpub_date
1998-03-01 00:00:00pages
125-30issue
2eissn
0920-9069issn
1573-0778journal_volume
26pub_type
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