Hypoxia enhances proliferation and stemness of human adipose-derived mesenchymal stem cells.

abstract：:Temperature reduction in CHO cell batch culture may be beneficial in the production of recombinant protein and in maintenance of viability. The effects on cell cycle, apoptosis and nucleotide pools were studied in cultures initiated at 37°C and temperature shifted to 30 °C after 48 hours. In control cultures maintaine...

journal_title：Cytotechnology

authors： Moore A,Mercer J,Dutina G,Donahue CJ,Bauer KD,Mather JP,Etcheverry T,Ryll T

更新日期：1997-01-01 00:00:00

abstract：:To characterize the effects of cetyltriethylammonium bromide (CTAB) on the transcription of gp64 promoter from Bombyx mori nucleopolyhedrovirus (BmNPV), the plasmid pBmgp64Luc used in transient expression assay system was constructed by using the luciferase gene as a reporter under the control of BmNPV gp64 promoter. ...

journal_title：Cytotechnology

authors： Zhou Y,Yi Y,Zhang Z,He J,Zhang Y

更新日期：2003-01-01 00:00:00

abstract：:Transient gene expression (TGE) bioprocesses have been difficult to scale up in large stirred tank bioreactors with volumes of more than 1.5 L. Low production levels are often observed, but the causes have not been investigated (Gutierrez-Granados et al. in Crit Rev Biotechnol 38:918-940, 2018). Chikungunya Virus-like...

journal_title：Cytotechnology

authors： Chen P,Demirji J,Ivleva VB,Horwitz J,Schwartz R,Arnold F

更新日期：2019-12-01 00:00:00

abstract：:Olive oil intake has been shown to induce significant levels of apoptosis in various cancer cells. These anti-cancer properties are thought to be mediated by phenolic compounds present in olive. These beneficial health effects of olive have been attributed, at least in part, to the presence of oleuropein and hydroxyty...

journal_title：Cytotechnology

authors： Han J,Talorete TP,Yamada P,Isoda H

更新日期：2009-01-01 00:00:00

abstract：:Disabled Infectious Single Cycle (DISC) HSV-2 has been cultured in the complimentary cell line CR2 to provide high titre bulk material suitable for the purification of the virus as a live viral vaccine. CR2 cells are cultured on the microcarrier Cytodex-1 at 5 g l-1 in small scale (1 l) and larger scale (15 l) reactor...

journal_title：Cytotechnology

authors： Zecchini TA,Smith RJ

更新日期：1999-07-01 00:00:00

abstract：:This study describes two packed bed bioreactor configurations which were used to culture a mouse-mouse hybridoma cell line (ATCC HB-57) which produces an IgG1 monoclonal antibody. The first configuration consists of a packed column which is continuously perfused by recirculating oxygenated media through the column. In...

journal_title：Cytotechnology

authors： Wang G,Zhang W,Jacklin C,Freedman D,Eppstein L,Kadouri A

更新日期：1992-01-01 00:00:00

abstract：:Structure-activity relationship of antioxidants for the protective effects on linoleic acid hydroperoxide (LOOH)-induced toxicity were examined in cultured human umbilical vein endothelial cells. alpha-Tocopherol, 2,2,5,7,8-pentamethylchroman-6-ol, butylated hydroxytoluene, probucol, and fatty acid esters of ascorbic ...

journal_title：Cytotechnology

authors： Kaneko T,Baba N,Matsuo M

更新日期：2001-01-01 00:00:00

abstract：:The application of image analysis methods to conventional thin sections for electron microscopy to analyze the chromatin arrangement are quite limited. We developed a method which utilizes freeze-fractured samples; the results indicate that the method is suitable for identifying the changes in the chromatin arrangemen...

journal_title：Cytotechnology

authors： Maraldi NM,Marinelli F,Squarzoni S,Santi S,Barbieri M

更新日期：1991-02-01 00:00:00

abstract：:We have studied parameters for optimizing the Spodoptera frugiperda (Sf9) cell culture and viral infection for the production of Anticarsia gemmatalis multiple nucleopolyhedrosis virus (AgMNPV) polyhedra inclusion bodies (PIBs) in shaker-Schott or spinner bottles and bioreactors. We have assayed the k(L)a of the syste...

journal_title：Cytotechnology

authors： Rodas VM,Marques FH,Honda MT,Soares DM,Jorge SA,Antoniazzi MM,Medugno C,Castro ME,Ribeiro BM,Souza ML,Tonso A,Pereira CA

更新日期：2005-06-01 00:00:00

abstract：:Gingival fibroblast cultures from four patients with Down's Syndrome (DS) and periodontal disease were compared with four in vitro age-matched fibroblast cultures of handicapped subjects (ND) also affected by periodontitis. The extra copy of chromosome 21 could alter growth regulation and biochemical mechanisms, so we...

journal_title：Cytotechnology

authors： Solmi R,Rossetti A,Talassi O,Tomasini GL,Fato R,Estornell E,Lucarini G,Lenaz G,Simonelli L,Brunelli MA

更新日期：1993-01-01 00:00:00

abstract：:In the last 10 years, new assignments and the special demands of mammalian cells to the culture conditions caused the development of complex small scale fermentation setups. The use of continuous fermentation and cell retention devices requires appropriate process control systems. An arrangement for control and data-a...

journal_title：Cytotechnology

authors： Büntemeyer H,Marzahl R,Lehmann J

更新日期：1994-01-01 00:00:00

abstract：:There may exist a connection between Echinococcus granulosus infection and cancer development. Here, it is aimed to investigate specific effects of E. granulosus protoscoleces (PSCs) on the proliferation and invasion capacities of hepatocellular carcinoma (HCC) cells in vitro and ex vitro. HepG2 cells were cultured wi...

journal_title：Cytotechnology

authors： Yasen A,Wang M,Ran B,Lv G,Aji T,Xiao H,Shao Y,Wen H

更新日期：2021-02-01 00:00:00

abstract：:A Xenopus laevis peptidyl C-terminal alpha-amidating enzyme (AE-II) gene, modified by deletion of a region encoding the putative membrane-spanning domain and the putative C-terminal cytosolic tail, was expressed in BoMo-15 AIIc insect cells and silkworm larvae using a Bombyx mori baculovirus expression vector system. ...

journal_title：Cytotechnology

authors： Kobayashi J,Imanishi S,Inoue H,Ohsuye K,Yamaichi K,Tsuruoka N,Tanaka S

更新日期：1992-01-01 00:00:00

abstract：:The relatively recent discovery of miRNAs has added a completely new dimension to the study of the regulation of gene expression. The mechanism of action of miRNAs, the conservation between diverse species and the fact that each miRNA can regulate a number of targets and phenotypes clearly indicates the importance of ...

journal_title：Cytotechnology

authors： Gammell P

更新日期：2007-04-01 00:00:00

abstract：:Effective harvesting procedure for adipose tissue is demanded by the affordable Good Manufacturing Practice-Compliant Production of clinical-grade adipose tissue-derived stem cells (hADSCs). Enzymatic digestion using collagenase is the most reliable method of adipose tissue-derived stem cells (hADSCs) isolation, while...

journal_title：Cytotechnology

authors： Li Z,Mu D,Liu C,Xin M,Fu S,Li S,Qi J,Wang Q,Luan J

更新日期：2020-04-01 00:00:00

abstract：:Primary cell cultures obtained from embryos of Mizuchopecten yessoensis (Bivalvia) survived for four months. Although the number of cells progressively decreased during the cultivation, mitotic cells were observed both at the first stages and at the end. A possibility of growing marine invertebrates cells in long term...

journal_title：Cytotechnology

authors： Odintsova NA,Khomenko AV

更新日期：1991-05-01 00:00:00

abstract：:Sufentanil, a lipophilic opioid, is the most frequently used clinical drug for ischemic heart disease. The effects of sufentanil on MAPK signaling in ischemic heart disease were explored. The effects of sufentanil on ischemia-reperfusion (IR)-induced myocardial injury in a rat model were examined. The serum levels of ...

journal_title：Cytotechnology

authors： Tao H,Nuo M,Min S

更新日期：2018-02-01 00:00:00

abstract：:A new bioreactor for animal cell cultivation employs two compartments for cells and medium respectively. The two chambers are separated by an ultrafiltration membrane. Cells and solution of collagen or collagen/chitosan mixture were loaded to the cell chamber and were allowed to form gel inside. Contraction of the cel...

journal_title：Cytotechnology

authors： Scholz M,Hu WS

更新日期：1990-09-01 00:00:00

abstract：:This paper reviews the JRC-Ispra research activity on toxicological studies related to the environmental and occupational exposure of trace metals, as carried out by a combination of cell culture methodologies with nuclear and radioanalytical techniques. Applications concern the setting of uptake-effect relationships ...

journal_title：Cytotechnology

authors： Sabbioni E,Pozzi G,Fischbach M,Bahbouth E

更新日期：1993-01-01 00:00:00

abstract：:Drug uptake and efflux are two of the critical factors required in order to be able to define drug efficacy. This study aims to investigate cytotoxicity and uptake mechanisms of two FL118 analogues (7-Q20 and val-FL118) in parallel with FL118 in three-dimensional multi-cellular spheroids model. The influence of compou...

journal_title：Cytotechnology

authors： Zhou L,Weng Q,Zheng Y,Zhou Y,Li Q,Li F

更新日期：2019-07-12 00:00:00

abstract：:The bone marrow represents the most common source from which to isolate mesenchymal stem cells (MSCs). MSCs are capable of differentiating into tissues of the three primary lineages and have the potential to enhance repair in damaged organs through the principals of regenerative medicine. Given the ease with which MSC...

journal_title：Cytotechnology

authors： Dickinson H,Milton P,Jenkin G

更新日期：2012-10-01 00:00:00

abstract：:Reproductive cells are a very special kind of material for the analysis. Depending on the species, their dimensions allow for the application of mass spectrometry imaging-based techniques to receive a reasonable data for interpretation of their condition without any additional sample preparation steps, except for typi...

journal_title：Cytotechnology

authors： Bodzon-Kulakowska A,Arena R,Mielczarek P,Hartman K,Kozoł P,Gibuła-Tarlowska E,Wrobel TP,Gąsior Ł,Polański Z,Ptak GE,Suder P

更新日期：2020-06-01 00:00:00

abstract：:The chicken is a useful animal for the development of the specificantibodies against the mammalian conserved proteins. We generated twotypes of recombinant chicken monoclonal antibodies (mAbs), using a phagedisplay technique from a chicken hybridoma HUC2-13 which secreted themAb to the N-terminal of the mammalian prio...

journal_title：Cytotechnology

authors： Nakamura N,Aoki Y,Horiuchi H,Furusawa S,Yamanaka HI,Kitamoto T,Matsuda H

更新日期：2000-03-01 00:00:00

abstract：:Increasing interest has been observed in the use of photobiomodulation (PBM) to enhance the proliferation of stem cells and induce their differentiation. The effects of PBM at two different wavelengths (635 and 809 nm) with three different energy densities (0.5, 1 and 2 J/cm2) on the osteogenic differentiation of adip...

journal_title：Cytotechnology

authors： Bölükbaşı Ateş G,Ak A,Garipcan B,Gülsoy M

更新日期：2020-04-01 00:00:00

abstract：:In our previous work, we isolated Arbas Cashmere goat hair follicle stem cells (gHFSCs) and explored the pluripotency. In this study, we investigated the expression and putative role of Sox9 in the gHFSCs. Immunofluorescence staining showed that Sox9 is predominantly expressed in the bulge region of the Arbas Cashmere...

journal_title：Cytotechnology

authors： He N,Dong Z,Tai D,Liang H,Guo X,Cang M,Liu D

更新日期：2018-08-01 00:00:00

abstract：:The use of the gadd153promoter to induce expression of a reporter geneunder heat stress conditions was investigated,since the results of previous studies have suggestedthat the gadd153promoter is likely to be activated by the indirecteffects of hyperthermia, that is, by DNA damage thatoccurs when reactive oxygen speci...

journal_title：Cytotechnology

authors： Bouhon IA,Ito A,Shinkai M,Honda H,Kobayashi T

更新日期：2000-07-01 00:00:00

abstract：:The ability to accurately determine cell viability is essential to performing a well-controlled biological experiment. Typical experiments range from standard cell culturing to advanced cell-based assays that may require cell viability measurement for downstream experiments. The traditional cell viability measurement ...

journal_title：Cytotechnology

authors： Chan LL,Kuksin D,Laverty DJ,Saldi S,Qiu J

更新日期：2015-05-01 00:00:00

abstract：:In the present study, we isolated preadipocytes from the adipose tissue of Peking duck and subsequently cultured them in vitro. Cell counting kit-8 assay was employed to establish the growth curve of duck primary preadipocytes. Meanwhile, after the cells reaching full confluency, they were induced to differentiate int...

journal_title：Cytotechnology

authors： Ding F,Li QQ,Li L,Gan C,Yuan X,Gou H,He H,Han CC,Wang JW

更新日期：2015-10-01 00:00:00

abstract：:In this investigation, clastogenic effects of Thymus kotschyanus var. glabrescens Boiss. extract (TE) and anticlastogenic effects of this extract against Mitomycin C (MMC) induced chromosome damage have been evaluated in human peripheral blood lymphocytes in vitro. Two series of experiments were conducted. In the firs...

journal_title：Cytotechnology

authors： Celik M,Unal F,Yüzbaşıoğlu D,Yılmaz S,Aksoy H,Karaman S

更新日期：2006-06-01 00:00:00

abstract：:Many cytological studies have reported that the numbers of binucleated cells were elevated in various tumors. However, binucleated cells are observed in not only malignant tumors but also normal tissues. Thus, the clinical significance of binucleated cells is controversial. Here we attempted to elucidate the character...

journal_title：Cytotechnology

authors： Nishimura K,Watanabe S,Hayashida R,Sugishima S,Iwasaka T,Kaku T

更新日期：2016-08-01 00:00:00