Heat induction of reporter gene expression via the gadd153 promoter and its possible application to hyperthermia treatment of cancer.

abstract：:Specific respiration rate ( [Formula: see text]) is a key parameter to understand cell metabolism and physiological state, providing useful information for process supervision and control. In this work, we cultivated different insect cells in a very controlled environment, being able to measure [Formula: see text]. Sp...

journal_title：Cytotechnology

authors： Pamboukian MM,Jorge SA,Santos MG,Yokomizo AY,Pereira CA,Tonso A

更新日期：2008-05-01 00:00:00

abstract：:We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum free media and suspension growth. Subsequent...

journal_title：Cytotechnology

authors： Schoofs G,Monica TJ,Ayala J,Horwitz J,Montgomery T,Roth G,Castillo FJ

更新日期：1998-11-01 00:00:00

abstract：:Human skin has continuous self-renewal potential throughout adult life and serves as first line of defence. Its cellular components such as human epidermal keratinocytes (HEKs) and dermal mesenchymal stromal cells (DMSCs) are valuable resources for wound healing applications and cell based therapies. Here we show a si...

journal_title：Cytotechnology

authors： Mahabal S,Konala VB,Mamidi MK,Kanafi MM,Mishra S,Shankar K,Pal R,Bhonde R

更新日期：2016-08-01 00:00:00

abstract：:Light scattering properties of hybridoma cells were examined with flow cytometry. Viable and dead cells form two distinct populations. The distribution of the two populations changes during a batch culture. the concentration of dead cells measured by flow cytometry correlates well to that measured by hemacytometer. Th...

journal_title：Cytotechnology

authors： Sen S,Srienc F,Hu WS

更新日期：1989-05-01 00:00:00

abstract：:This study demonstrated a newly developed method using adipose tissue-derived stromal cells (ADSCs) and hydroxypropylmethylcellulose (HPMC) in building injectable tissue engineered cartilage in vivo. ADSCs from rabbit subcutaneous fatty tissue were cultured in chondrogenic differentiation medium and supplemented with ...

journal_title：Cytotechnology

authors： Xu Y,Zhang J,Ma Y,Han Y,Min J,Liang Y,Zhao D,Qiu J

更新日期：2014-10-01 00:00:00

abstract：:Dental pulp stem cells (DPSCs) are a type of mesenchymal stem cells, which have the self-renewal and multi-lineage differentiation potential, including chondrocytes, adipocytes, neural cells and osteoblasts. So they play a significant role in pulp repair and bone regeneration. Oncostatin M (OSM), one of the IL-6 famil...

journal_title：Cytotechnology

authors： Feng X,Shen S,Cao P,Zhu L,Zhang Y,Zheng K,Feng G,Zhang D

更新日期：2016-12-01 00:00:00

abstract：:Chemical genomics is a new research paradigm with importantapplications in drug discovery. It links genomic targets withsmall-molecule chemistries thereby allowing for efficient targetvalidation and lead compound identification. ACADIA'schemical-genomics platform consists of a large and diverse small-moleculelibrary (...

journal_title：Cytotechnology

authors： Hacksell U,Nash N,Burstein ES,Piu F,Croston G,Brann MR

更新日期：2002-01-01 00:00:00

abstract：:Helichrysum Mill. (Asteraceae) species have been used in folk medicine for thousands of years in the world. The in vitro cytogenetic effects in human lymphocytes of nine Helichrysum taxa used in Turkey folk medicine were investigated. Blood samples were obtained from healthy donors, non-smoking volunteers, which were ...

journal_title：Cytotechnology

authors： Eroğlu HE,Aksoy A,Hamzaoğlu E,Budak U,Albayrak S

更新日期：2009-01-01 00:00:00

abstract：:Oxidative stress plays a vital role in the occurrence and development of intestinal injury. Soluble epoxide hydrolase 2 gene (EPHX2) is a class of hydrolytic enzymes. We aim to explore the effects and molecular mechanism of siEPHX2 on H2O2-induced oxidative damage in rat intestinal epithelial IEC-6 cells. IEC-6 cells ...

journal_title：Cytotechnology

authors： Li J,Luo J,Zhang Y,Tang C,Wang J,Chen C

更新日期：2020-02-01 00:00:00

abstract：:We screened for biological activity which induces neurite outgrowth in vitro from 300 species of marine algae from along the Japan coast for possible use as a treatment for the lack of neurotrophic factor which is considered to be a cause of Alzheimer's disease. In this study, we evaluated the neurite outgrowth promot...

journal_title：Cytotechnology

authors： Kamei Y,Sagara A

更新日期：2002-11-01 00:00:00

abstract：:Cells derived from the periodontal ligament (PDL) have previously been reported to have stem cell-like characteristics (PDL stem cells; PDLSCs) and play an important part in bone engineering, including that of alveolar bone. However, these populations have been heterogeneous, and thus far no specific marker has yet be...

journal_title：Cytotechnology

authors： Sununliganon L,Singhatanadgit W

更新日期：2012-01-01 00:00:00

abstract：:In neuronal dendrites, septins localize to the base of the spine, a unique position which is sandwiched between the microtubule (MT)-rich dendritic shaft and the actin filament-rich spine. Here, we provide evidence for the association of SEPT6 with MTs in cultured rat hippocampal neurons. In normal cultures, SEPT6 clu...

journal_title：Cytotechnology

authors： Moon IS,Lee H,Walikonis RS

更新日期：2013-03-01 00:00:00

abstract：:Numerous challenges remain in the successful clinical translation of cell-based therapeutic studies for skeletal tissue repair, including appropriate cell sources and viable cell delivery systems. Poly(ethylene glycol)-poly(ε-caprolactone) (PEG-PCL) amphiphilic block copolymers have been extensively explored in micros...

journal_title：Cytotechnology

authors： Zhang D,Tong A,Zhou L,Fang F,Guo G

更新日期：2012-12-01 00:00:00

abstract：:Despite the fact, that monoclonal antibodies are the fastest growing group of biopharmaceuticals in development, this is not true for the IgM class, which remains as enigmatic as ever. While more examples of usefulness of IgMs for medical applications are emerging, their recombinant production is still not common. In ...

journal_title：Cytotechnology

authors： Chromikova V,Mader A,Steinfellner W,Kunert R

更新日期：2015-03-01 00:00:00

abstract：CONCLUSIONS:The BEVS has become widely utilized for production of recombinant proteins. However, protein aggregation and inefficient processing often limit yields, especially for secreted and membrane proteins. Since many proteins of pharmaceutical interest require similar posttranslational processing steps, engineerin...

journal_title：Cytotechnology

authors： Betenbaugh MJ,Ailor E,Whiteley E,Hinderliter P,Hsu TA

更新日期：1996-01-01 00:00:00

abstract：:Daphnoretin is a bicoumarin compound isolated from a natural product, Wikstroemia indica, which has been used to treat many diseases. It has strong antiviral and anti-tumor activities. Taking the anti-tumor activity of daphnoretin as a starting point, the present study aimed to test the pro-apoptotic effect of daphnor...

journal_title：Cytotechnology

authors： Yang ZY,Kan JT,Cheng ZY,Wang XL,Zhu YZ,Guo W

更新日期：2014-01-01 00:00:00

abstract：:Several lichen species have been used for medicinal purposes throughout the ages, and they are reported to be effective in the treatment of different disorders including ulcer and cancer. It is revealed that lichens may be easily accessible sources of natural drugs and possible food supplements after their safety eval...

journal_title：Cytotechnology

authors： Turkez H,Aydin E,Aslan A

更新日期：2012-12-01 00:00:00

abstract：:The development of specific catalytic inhibitors for the serine protease urokinase-type plasminogen activator (uPA) has been hindered due to difficulties in producing sufficient amounts of active recombinant uPA that is catalytically equivalent to native uPA. The purpose of this study was to develop an efficient syste...

journal_title：Cytotechnology

authors： Atkins E,Zamora S,Candia BJ,Baca A,Orlando RA

更新日期：2005-09-01 00:00:00

abstract：:This study describes two packed bed bioreactor configurations which were used to culture a mouse-mouse hybridoma cell line (ATCC HB-57) which produces an IgG1 monoclonal antibody. The first configuration consists of a packed column which is continuously perfused by recirculating oxygenated media through the column. In...

journal_title：Cytotechnology

authors： Wang G,Zhang W,Jacklin C,Freedman D,Eppstein L,Kadouri A

更新日期：1992-01-01 00:00:00

abstract：:Effective harvesting procedure for adipose tissue is demanded by the affordable Good Manufacturing Practice-Compliant Production of clinical-grade adipose tissue-derived stem cells (hADSCs). Enzymatic digestion using collagenase is the most reliable method of adipose tissue-derived stem cells (hADSCs) isolation, while...

journal_title：Cytotechnology

authors： Li Z,Mu D,Liu C,Xin M,Fu S,Li S,Qi J,Wang Q,Luan J

更新日期：2020-04-01 00:00:00

abstract：:Multidrug resistance (MDR) is a major problem in cancer chemotherapy. As P-glycoprotein is the key molecule in MDR, many investigators have constructed anti-P-glycoprotein monoclonal antibodies (MAbs). Those antibodies, including MRK16 and C219, were used for elucidation of the mechanism of MDR and for overcoming of M...

journal_title：Cytotechnology

authors： Heike Y,Tsuruo T

更新日期：1993-01-01 00:00:00

abstract：:As chemical pesticides are being banned as control agents for agricultural pests, the use of the highly specific, safe to non-target organisms baculoviruses has been proposed. These viruses can be produced either in vivo or in vitro. In vitro production requires appropriated host insect cell lines with the ability for...

journal_title：Cytotechnology

authors： Beas-Catena A,Sánchez-Mirón A,García-Camacho F,Molina-Grima E

更新日期：2011-12-01 00:00:00

abstract：:The neurite outgrowth of PC12 cells on collagen-coated glass plates under light emitting diode (LED) irradiation at several wavelengths (i.e., 455, 470, 525, 600, 630, 880 and 945 nm) was investigated. No neurite outgrowth was observed during cultivation under irradiation from the lamp of an inverted light microscope ...

journal_title：Cytotechnology

authors： Higuchi A,Watanabe T,Noguchi Y,Chang Y,Chen WY,Matsuoka Y

更新日期：2007-07-01 00:00:00

abstract：:Galangin, an active flavonoid present at high concentration in Alpinia officinarum Hance and propolis, shows cytotoxicity towards several cancer cell lines, including melanoma. However, the specific cellular targets of galangin-induced cytotoxicity in melanoma are still unknown. Here, we investigated the effects of ga...

journal_title：Cytotechnology

authors： Zhang W,Lan Y,Huang Q,Hua Z

更新日期：2013-05-01 00:00:00

abstract：:The presence of a highly purified recombinant interleukin-2 (rIL-2) increased the production of immunoglobulin (IgM or IgG) by human-human hybridomas to 1.5-2.0 times the production by untreated cells. However, these cells did not react with anti-Tac (IL-2 receptor alpha) antibody. To enhance the response of the hybri...

journal_title：Cytotechnology

authors： Iwamoto K,Shintani Y,Sasada R,Honjo T,Kitano K

更新日期：1990-07-01 00:00:00

abstract：:Previously, it has been shown that the binding of epidermal growth factor (EGF) by a wide range of cells decreases as cell density increases. In this report, we demonstrate that KB cells treated chronically with phorbol esters continue to exhibit decreases in EGF receptor binding as cell density increases. This findin...

journal_title：Cytotechnology

authors： Rizzino A,Huebert C,Kuszynski C,Wilder PJ

更新日期：1991-10-01 00:00:00

abstract：:Material and degree of reductance balance equations are used to estimate the rates of oxygen uptake and carbon dioxide evolution of animal cell cultures. Lumped compositions, molecular weight and reductance degree of cellular protein, monoclonal antibody, biomass and amino acid consumption (excluding glutamine and ala...

journal_title：Cytotechnology

authors： Xiu ZL,Deckwer WD,Zeng AP

更新日期：1999-05-01 00:00:00

abstract：:The benzofuran lignans egonol and homoegonol are found in all species of the genus Styrax. Since natural products are important sources of new anticancer drugs, this study evaluated the cytotoxic activity of a hydroalcoholic extract of the stems of S. camporum (SCHE) and their chemical markers, egonol (EG) and homoego...

journal_title：Cytotechnology

authors： de Oliveira PF,Damasceno JL,Bertanha CS,Araújo AR,Pauletti PM,Tavares DC

更新日期：2016-08-01 00:00:00

abstract：:We developed a simple method for freezing anchorage-dependent cells, including primary cultured rat liver parenchymal cells, without detaching the cells from the culture dish. The method consists of preculture of the cells to confluence, changing the growth medium to a conventional freezing medium, packaging in a cont...

journal_title：Cytotechnology

authors： Ohno T,Saijo-Kurita K,Miyamoto-Eimori N,Kurose T,Aoki Y,Yosimura S

更新日期：1991-03-01 00:00:00

abstract：:Statins may have beneficial effects in atherogenesis given their antithrombotic properties involving non-lipid mechanisms that modify endothelial function of tissue factor induction by thrombin. In this study, we investigate the effect of atorvastatin on tissue factor (TF) activity in thrombin-stimulated endothelial c...

journal_title：Cytotechnology

authors： Martínez-Sales V,Vila V,Ferrando M,Reganon E

更新日期：2011-01-01 00:00:00