Abstract:
:A Xenopus laevis peptidyl C-terminal alpha-amidating enzyme (AE-II) gene, modified by deletion of a region encoding the putative membrane-spanning domain and the putative C-terminal cytosolic tail, was expressed in BoMo-15 AIIc insect cells and silkworm larvae using a Bombyx mori baculovirus expression vector system. The expressed enzyme was identified predominantly in the culture medium and the hemolymph of silkworm larvae, indicating successful secretion of the expressed AE-II. The level of recombinant enzyme in the larval hemolymph at 4 days post-infection (40 micrograms/ml) was more than 100-fold the peak levels found in the culture medium (250 ng/ml). The enzyme activity in the larval hemolymph at 4 days post-infection was 3700 units/ml.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Kobayashi J,Imanishi S,Inoue H,Ohsuye K,Yamaichi K,Tsuruoka N,Tanaka Sdoi
10.1007/BF02525492subject
Has Abstractpub_date
1992-01-01 00:00:00pages
103-8issue
2eissn
0920-9069issn
1573-0778journal_volume
8pub_type
杂志文章相关文献
CYTOTECHNOLOGY文献大全abstract::In the present manuscript, the methods required to generate purified cultures of mature adipocytes, as well as stromal vascular cells, from the same isolation are detailed. Also, we describe the in vitro conditions for the dedifferentiation of the isolated mature adipocytes. These two types of cells may be used to ree...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-005-2602-0
更新日期:2004-10-01 00:00:00
abstract::More than 35 years ago double Nobel laureate Linus Carl Pauling published a powerful model of the molecular mechanism of general anesthesia, generally referred to as the hydrate-microcrystal (aqueous-phase) theory. This hypothesis, based on the molecular behavior of water molecules, did not receive serious attention d...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-009-9216-x
更新日期:2009-04-01 00:00:00
abstract::This study was undertaken to evaluate genotoxic potential of Thermopsis turcica aqueous extracts on the roots of onion bulb (Allium cepa L.) by comet assay and random amplified polymorphic DNA technique. The Allium root growth inhibition test indicated that the EC50 and 2×EC50 values were 8 and 16 mg/ml concentrations...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-014-9835-8
更新日期:2016-08-01 00:00:00
abstract::The statistics of estimators used with the endpoint assay for virus titration were investigated. For a standard assay with 10 wells/dilution, the graphical estimator traditionally used was found to produce estimates with significant positive bias and a relatively low accuracy. Furthermore, the graphical estimator was ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF02522040
更新日期:1992-01-01 00:00:00
abstract::This article describes the historical development of the prophylactic use of antibiotics in cell culture as well as their effects on cells. The influence of antibiotics on cell morphology, cellular degeneration and cell death and cellular function is summarized. Cellular DNA as well as protein synthesis are affected w...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00749764
更新日期:1995-06-01 00:00:00
abstract::The culture of pulmonary arterial smooth muscle cells (PASMCs) is one of the most powerful tools for exploring the mechanisms of pulmonary hypertension (PH). Both pulmonary vasoconstriction and remodeling occur predominantly in distal pulmonary arteries (PA). In this study, we provide our detailed and standardized pro...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-017-0081-8
更新日期:2017-10-01 00:00:00
abstract::The bone marrow represents the most common source from which to isolate mesenchymal stem cells (MSCs). MSCs are capable of differentiating into tissues of the three primary lineages and have the potential to enhance repair in damaged organs through the principals of regenerative medicine. Given the ease with which MSC...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-012-9443-4
更新日期:2012-10-01 00:00:00
abstract::The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rb1 was produced by fusing splenocytes immunized with a ginsenoside Rb1- bovine serum albumin conjugate wit...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008068718392
更新日期:1999-03-01 00:00:00
abstract::Fibroblast growth factor 21 (FGF21) potentially regulates glucose and lipid metabolism in energy homeostasis. We investigated dynamic changes in goat adipocytes treated with 75 nM FGF21 for 24, 36 and 48 h. Compared to controls, FGF21-treated adipocytes displayed smaller lipid droplets and altered levels of the mRNA t...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-018-0237-1
更新日期:2018-10-01 00:00:00
abstract::Precision-cut liver tissue slices (PCLS) have been used for decades to study pharmacological metabolism as well as toxicology and efficacy of novel substances on primary material under standardized conditions. Slicing of primary liver tissue has been done using different slicing machines. Since there has been great va...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-009-9246-4
更新日期:2009-12-01 00:00:00
abstract::To characterize the effects of cetyltriethylammonium bromide (CTAB) on the transcription of gp64 promoter from Bombyx mori nucleopolyhedrovirus (BmNPV), the plasmid pBmgp64Luc used in transient expression assay system was constructed by using the luciferase gene as a reporter under the control of BmNPV gp64 promoter. ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1024231023015
更新日期:2003-01-01 00:00:00
abstract::The aim of this research was a comparative study on the isolation and culture of head kidney macrophages derived from Acipenser persicous and Rutilus frisii kutum as teleost and chondrostei species of fish. The macrophages were isolated by density gradient sedimentation, followed by adherence to a plastic surface. The...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-018-0256-y
更新日期:2018-12-01 00:00:00
abstract::A novel type of succinoyl trehalose lipid (STL-1) prepared from n-hexadecane-culture of Rhodococcus erythropolis SD-74 markedly inhibited the growth of a human monocytoid leukemic cell line, U937, and induced its morphological alteration along a monocyte-macrophage lineage. STL-1 markedly increased differention-associ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00749758
更新日期:1995-01-01 00:00:00
abstract::We developed a simple method for freezing anchorage-dependent cells, including primary cultured rat liver parenchymal cells, without detaching the cells from the culture dish. The method consists of preculture of the cells to confluence, changing the growth medium to a conventional freezing medium, packaging in a cont...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00556297
更新日期:1991-03-01 00:00:00
abstract::In neuronal dendrites, septins localize to the base of the spine, a unique position which is sandwiched between the microtubule (MT)-rich dendritic shaft and the actin filament-rich spine. Here, we provide evidence for the association of SEPT6 with MTs in cultured rat hippocampal neurons. In normal cultures, SEPT6 clu...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-012-9477-7
更新日期:2013-03-01 00:00:00
abstract::The polymerase chain reaction (PCR) has been used for the general detection of Mollicutes. 25 Mycoplasma and Acholeplasma species were detected including important contaminants of cell cultures such as M. orale, M. arginini, M. hyorhinis, M. fermentans, A. laidlawii and additional human and animal mycoplasmas. PCR rea...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00754609
更新日期:1994-01-01 00:00:00
abstract::Photopolymerizable hydrogels offer great potential in cartilage tissue engineering due to their ability to conform to irregular defect shapes and be applied in a potentially minimally invasive manner. An important process requirement in the use of photopolymerizable hydrogels is the ability of the suspended cells to w...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-012-9512-8
更新日期:2013-08-01 00:00:00
abstract::Cells transfected by retroviral vectors are brought in agene of particular interest and are very useful in avariety of experiments. It is essential to testify that theDNA fragment was successfully introduced into the cellstogether with the retroviral vectors. Polymerase chainreaction is believed to be a fast and conve...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008187310534
更新日期:2000-11-01 00:00:00
abstract::The aim of this study was to reveal the biological activities and in vivo toxicity profiles of n-hexane, chloroform and methanol extracts of brown algae Halopteris scoparia L. Sauvageau. In this study, extracts were tested for their phytochemical contents and antioxidant activities. The cytotoxic activities of the ext...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-019-00314-5
更新日期:2019-06-01 00:00:00
abstract::The current study was conducted to determine whether there is a relation between hypertension and two different polymorphisms, including C1562T of the Matrix metalloproteinase-9 (MMP-9) gene and C677T of the methylenetetrahydrofolate reductase (MTHFR) gene. Genomic DNA obtained from 224 persons (125 patients with hype...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-013-9665-0
更新日期:2015-01-01 00:00:00
abstract::Arrowroot (Maranta arundinacea. L) is an underutilized local crop potentially to be developed as carbohydrate source and functional food in Indonesia. The objectives of this research are to evaluate the immunostimulatory effects of arrowroot extracts in vitro by using animal cell culture techniques, and in vivo by usi...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-011-9403-4
更新日期:2012-03-01 00:00:00
abstract::Carvacrol, the predominant monoterpene in many essential oils of Labitae including Origanum, Satureja, Thymbra, Thymus, and Corydothymus has substantial antibacterial, antifungal, antihelmintic, insecticial, analgesic and antioxidant activities. Approximately 75% of lung cancer is non-small cell carcinoma (NSCLC) whic...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/b:cyto.0000039917.60348.45
更新日期:2003-11-01 00:00:00
abstract::Hepatocytes self-assemble in culture to form compacted spherical aggregates, or spheroids, that mimic the structure of the liver by forming tight junctions and bile canalicular channels. Hepatocyte spheroids thus resemble the liver to a great extent. However, liver tissue contains other cell types and has bile ducts a...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-004-7996-6
更新日期:2004-07-01 00:00:00
abstract::Mathematical models describing drug resistance are briefly reviewed. One model which describes the molecular function of the P-glycoprotein pump in multidrug resistant (MDR) cell lines has been developed and is presented in detail. The pump is modeled as an energy dependent facilitated diffusion process. A partial dif...
journal_title:Cytotechnology
pub_type: 杂志文章,评审
doi:10.1007/BF00744670
更新日期:1993-01-01 00:00:00
abstract::We have studied parameters for optimizing the Spodoptera frugiperda (Sf9) cell culture and viral infection for the production of Anticarsia gemmatalis multiple nucleopolyhedrosis virus (AgMNPV) polyhedra inclusion bodies (PIBs) in shaker-Schott or spinner bottles and bioreactors. We have assayed the k(L)a of the syste...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-005-3175-7
更新日期:2005-06-01 00:00:00
abstract::In recent years, water pollution has been converted to a challenging discussion in health area of human being. Heavy elements are one of the most important water pollutants and their negative adverse effects on body systems have been confirmed. In this study, investigation of effects of two heavy elements including le...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-013-9626-7
更新日期:2014-10-01 00:00:00
abstract::Sorbaria kirilowii (Regel) Maxim, a plant found in China, Korea, Japan, and east of Europe, is a common herb used for traditional medicinal purposes. However, its ability to prevent photoaging has not been studied. In this study, we investigated the anti-photoaging functions of an ethanol extract (Sk-EE) of S. kirilow...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-020-00449-w
更新日期:2021-02-01 00:00:00
abstract::In cartilage tissue engineering, viable cell numbers should be correctly counted in the collagenase digest of the biopsied cartilage. However, this is a difficult task due to the presence of matrix debris, cell ghosts and their aggregates. To search for the correct cell counting method in this situation, we evaluated ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-010-9304-y
更新日期:2010-12-01 00:00:00
abstract::We have developed a generic transient transfection process at 100 L scale, using HEK293-EBNA cells and PEI as the transfection reagent for the production of recombinant IgG. The process, including large-scale plasmid preparation, expression at bioreactor scale, capture, purification and, if necessary, endotoxin remova...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-008-9135-2
更新日期:2008-02-01 00:00:00
abstract::Hyperuricemia is an important risk factor for gout. Isorhamnetin (3'-O-methylquercetin) is an O-methylated flavonol, which occurs in onion, almond and sea buckthorn. It is also one of the metabolites of quercetin in mammals. In the present study, we investigated anti-hyperuricemic effect of isorhamnetin adopting both ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-018-0275-8
更新日期:2019-02-01 00:00:00