Cetyltriethylammonium bromide stimulating transcription of Bombyx mori nucleopolyhedrovirus gp64 gene promoter mediated by viral factors.


:To characterize the effects of cetyltriethylammonium bromide (CTAB) on the transcription of gp64 promoter from Bombyx mori nucleopolyhedrovirus (BmNPV), the plasmid pBmgp64Luc used in transient expression assay system was constructed by using the luciferase gene as a reporter under the control of BmNPV gp64 promoter. When the Bombyx mori cells (Bm-N) were transfected with the pBmgp64Luc, different treatments were undertaken. We found that the transient expression activity of luciferase could not be augmented directly by CTAB treatment alone, but could be enhanced more than 2 times by BmNPV treatment alone at a multiplicity of infection (MOI) of 0.5. Through co-treatment with 0.1 microg ml(-1) of CTAB and BmNPV at a MOI of 0.5, the enzymatic activity increased 5.21 times. We presumed that the stimulation of transcription of BmNPV gp64 promoter by CTAB was mediated by viral factors from BmNPV. In addition, the time curves of luciferase activity in cells transfected with pBmgp64Luc and transactivated by virus were observed.






Zhou Y,Yi Y,Zhang Z,He J,Zhang Y




Has Abstract


2003-01-01 00:00:00












  • Mechanisms of resistance to alkylating agents.

    abstract::Alkylating agents are the most widely used anticancer drugs whose main target is the DNA, although how exactly the DNA lesions cause cell death is still not clear. The emergence of resistance to this class of drugs as well as to other antitumor agents is one of the major causes of failure of cancer treatment. This pap...


    pub_type: 杂志文章


    authors: Damia G,D'Incalci M

    更新日期:1998-09-01 00:00:00

  • Modeling of cell culture processes.

    abstract::Models of cell processes can be particularly useful in simulating, optimizing and controlling cell culture systems. Models reported in the literature are of various degrees of biological structure and mathematical complexity and describe cell growth, death, metabolism, and product formation, alone or in combination wi...


    pub_type: 杂志文章,评审


    authors: Tziampazis E,Sambanis A

    更新日期:1994-01-01 00:00:00

  • Molecular mechanisms of Bombyx batryticatus ethanol extract inducing gastric cancer SGC-7901 cells apoptosis.

    abstract::Bombyx batryticatus is a traditional Chinese medicine. To understand apoptotic effect of B. batryticatus ethanol extract (BBE), we investigated the role of BBE in inducing apoptosis of human gastric cancer cells SGC-7901. Cells treated with BBE and apoptosis was assessed by methyl thiazolyl tetrazolium (MTT) assay, mo...


    pub_type: 杂志文章


    authors: Wu JY,Sheikho A,Ma H,Li TC,Zhao YQ,Zhang YL,Wang D

    更新日期:2017-12-01 00:00:00

  • Development of a Mammalian suspension culture for expression of active recombinant human urokinase-type plasminogen activator.

    abstract::The development of specific catalytic inhibitors for the serine protease urokinase-type plasminogen activator (uPA) has been hindered due to difficulties in producing sufficient amounts of active recombinant uPA that is catalytically equivalent to native uPA. The purpose of this study was to develop an efficient syste...


    pub_type: 杂志文章


    authors: Atkins E,Zamora S,Candia BJ,Baca A,Orlando RA

    更新日期:2005-09-01 00:00:00

  • Versatile stem cells, young and old. A review.

    abstract::Both embryonic and somatic stem cells have been studied in recent years with particular regard to their differentiation potential. In vitro studies allow a considerable amplification of such cells in culture as well as the induction of commitment in different directions under proper stimulating factors. Moreover, a su...


    pub_type: 杂志文章


    authors: Eridani S

    更新日期:2001-03-01 00:00:00

  • Anti-proliferative and apoptotic effects of oleuropein and hydroxytyrosol on human breast cancer MCF-7 cells.

    abstract::Olive oil intake has been shown to induce significant levels of apoptosis in various cancer cells. These anti-cancer properties are thought to be mediated by phenolic compounds present in olive. These beneficial health effects of olive have been attributed, at least in part, to the presence of oleuropein and hydroxyty...


    pub_type: 杂志文章


    authors: Han J,Talorete TP,Yamada P,Isoda H

    更新日期:2009-01-01 00:00:00

  • Effect of Partial Medium Replacement on Cell Growth and Protein Production for the High-Fivetrade mark insect cell line.

    abstract::The potential of spent medium to support the growth and recombinant protein production of High-Fivetrade mark cells was investigated. Growth in medium consisting of three parts fresh and one part spent medium was comparable to that in fresh medium (maximal specific growth rates of 0.028 and 0.029 h(-1), and maximal ce...


    pub_type: 杂志文章


    authors: Ikonomou L,Bastin G,Schneider YJ,Agathos SN

    更新日期:2004-01-01 00:00:00

  • Is there any genetic predisposition of MMP-9 gene C1562T and MTHFR gene C677T polymorphisms with essential hypertension?

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    pub_type: 杂志文章


    authors: Bayramoglu A,Urhan Kucuk M,Guler HI,Abaci O,Kucukkaya Y,Colak E

    更新日期:2015-01-01 00:00:00

  • Insights into protective effects of medium additives on animal cells under fluid stresses: the hydrophobic interactions.

    abstract::Animal cells in suspension culture can suffer severe mechanical damage from bursting gas bubbles or other hydrodynamic force sources. Certain chemical additives in the culture media, particularly some surface-active chemicals, can effectively protect animal cells against such damage. Previously we proposed that the pr...


    pub_type: 杂志文章


    authors: Wu J

    更新日期:1996-01-01 00:00:00

  • High-level expression of a foreign gene by a recombinant baculovirus with an expanded host range.

    abstract::The usefulness of host range expanded viruses as an expressionvector system was investigated by following the expression ofthe E. coli lacZ gene. The host range expanded recombinantviruses were obtained from Sf-21 or BmN-4 cells coinfected withAutographa californica and Bombyx mori nuclearpolyhedrosis viruses. Among t...


    pub_type: 杂志文章


    authors: Kim HS,Woo SD,Kim WJ,Choi JY,Kang SK

    更新日期:2000-02-01 00:00:00

  • Enhanced secretory ability for the human factor VIII light chain produced in baculovirus-infected insect cells.

    abstract::The light and truncated heavy chains of human factor VIII, expressed separately in baculovirus-infected insect cells, exhibited different secretory behaviour when compared with each other and with a biologically active fusion molecule of the truncated heavy and light chains. The light chain was very efficiently secret...


    pub_type: 杂志文章


    authors: Webb E,Tkalcevic J,Edwards S,Hocking D,Nisbet I

    更新日期:1996-01-01 00:00:00

  • Binucleated HeLa cells are formed by cytokinesis failure in starvation and keep the potential of proliferation.

    abstract::Many cytological studies have reported that the numbers of binucleated cells were elevated in various tumors. However, binucleated cells are observed in not only malignant tumors but also normal tissues. Thus, the clinical significance of binucleated cells is controversial. Here we attempted to elucidate the character...


    pub_type: 杂志文章


    authors: Nishimura K,Watanabe S,Hayashida R,Sugishima S,Iwasaka T,Kaku T

    更新日期:2016-08-01 00:00:00

  • Small interfering RNA knockdown of mini-TyrRS and mini-TrpRS effects angiogenesis in human umbilical vein endothelial cells in hypoxic culture.

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    pub_type: 杂志文章


    authors: Zeng R,Chen YC,Zeng Z,Liu R,Qiang O,Jiang XF,Liu XX,Li X,Wang HY

    更新日期:2008-03-01 00:00:00

  • Septin 6 localizes to microtubules in neuronal dendrites.

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    pub_type: 杂志文章


    authors: Moon IS,Lee H,Walikonis RS

    更新日期:2013-03-01 00:00:00

  • A direct computer control concept for mammalian cell fermentation processes.

    abstract::In the last 10 years, new assignments and the special demands of mammalian cells to the culture conditions caused the development of complex small scale fermentation setups. The use of continuous fermentation and cell retention devices requires appropriate process control systems. An arrangement for control and data-a...


    pub_type: 杂志文章


    authors: Büntemeyer H,Marzahl R,Lehmann J

    更新日期:1994-01-01 00:00:00

  • Towards the development of a bioartificial pancreas: immunoisolation and NMR monitoring of mouse insulinomas.

    abstract::A promising method for diabetes treatment is the implantation of immunoisolated cells secreting insulin in response to glucose. Cell availability limits the application of this approach at a medically-relevant scale. We explore the use of transformed cells that can be grown to large homogeneous populations in developi...


    pub_type: 杂志文章


    authors: Sambanis A,Papas KK,Flanders PC,Long RC Jr,Kang H,Constantinidis I

    更新日期:1994-01-01 00:00:00

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    abstract::The metabolism of hybridoma cells was controlled to reduce metabolic formation in fed-batch cultures by dynamically feeding a salt-free nutrient concentrate. For this purpose, on-line oxygen uptake rate (OUR) measurement was used to estimate the metabolic demand of hybridoma cells and to determine the feeding rate of ...


    pub_type: 杂志文章


    authors: Zhou W,Rehm J,Europa A,Hu WS

    更新日期:1997-07-01 00:00:00

  • Immunonucleochemistry: a new method for in situ detection of antigens in the nucleus of cells in culture.

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    authors: Moon IS,Lee H,Park SD,Seog DH

    更新日期:2010-04-01 00:00:00

  • Primary cell culture from embryos of the Japanese scallop Mizuchopecten yessoensis (Bivalvia).

    abstract::Primary cell cultures obtained from embryos of Mizuchopecten yessoensis (Bivalvia) survived for four months. Although the number of cells progressively decreased during the cultivation, mitotic cells were observed both at the first stages and at the end. A possibility of growing marine invertebrates cells in long term...


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    authors: Odintsova NA,Khomenko AV

    更新日期:1991-05-01 00:00:00

  • Aldehyde dehydrogenase activity of Wharton jelly mesenchymal stromal cells: isolation and characterization.

    abstract::Mesenchymal stromal cells (MSCs) are promising tools in regenerative medicine and targeted therapies. Although different origins have been described, there is still huge need to find a valuable source harboring specific subpopulations of MSCs with precise therapeutic functions. Here, we isolated by fluorescence activa...


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    authors: Najar M,Crompot E,van Grunsven LA,Dollé L,Lagneaux L

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  • Liver stem cells.

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    authors: Theise ND

    更新日期:2003-03-01 00:00:00

  • Expression of recombinant Atlantic salmon serum C-type lectin in Drosophila melanogaster Schneider 2 cells.

    abstract::The Atlantic salmon (Salmo salar) serum lectin (SSL) is a soluble C-type lectin that binds bacteria, including salmon pathogens. This lectin is a cysteine-rich oligomeric protein. Consequently, a Drosophila melanogaster expression system was evaluated for use in expressing SSL. A cDNA encoding SSL was cloned into a ve...


    pub_type: 杂志文章


    authors: Uribe E,Venkatesan M,Rose DR,Ewart KV

    更新日期:2013-08-01 00:00:00

  • Impact of chicken-origin cells on adaptation of a low pathogenic influenza virus.

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    pub_type: 杂志文章


    authors: Shahsavandi S,Ebrahimi MM,Mohammadi A,Zarrin Lebas N

    更新日期:2013-05-01 00:00:00

  • A plant extract which enhances the plating efficiency of lymphoid cell lines and enhances the survival of normal lymphoid cells in vitro.

    abstract::A water soluble extract from the bark of the Samoan medicinal plant Alphitonia zizyphoides A. Gray (Rhamnaceae), enhances the plating efficiency in vitro of lymphoid cell lines as well as the survival of bone marrow cells and normal T and B lymphocytes. Furthermore, the inclusion of bark-extract into culture media enh...


    pub_type: 杂志文章


    authors: Dunstan CA,Andersson J,Bohlin L,Cox PA,Grönvik KO

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  • Zoledronic acid suppresses metastasis of esophageal squamous cell carcinoma cells through upregulating the tight junction protein occludin.

    abstract::We have previously demonstrated the radio-sensitizing effect of zoledronic acid (ZOL), a third generation bisphosphonate, on human esophageal squamous cell carcinoma (ESCC) cells. Here we show that ZOL suppresses metastatic progression of ESCC cells mainly through up-regulating the tight junction protein occludin. Exp...


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    authors: Lin C,Xin S,Qin X,Li H,Lin L,You Y

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  • Photobiomodulation effects on osteogenic differentiation of adipose-derived stem cells.

    abstract::Increasing interest has been observed in the use of photobiomodulation (PBM) to enhance the proliferation of stem cells and induce their differentiation. The effects of PBM at two different wavelengths (635 and 809 nm) with three different energy densities (0.5, 1 and 2 J/cm2) on the osteogenic differentiation of adip...


    pub_type: 杂志文章


    authors: Bölükbaşı Ateş G,Ak A,Garipcan B,Gülsoy M

    更新日期:2020-04-01 00:00:00

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    pub_type: 杂志文章


    authors: Martinoia S,Meloni M,Parodi MT,Tedesco M,Ciccarelli C,Grattarola M

    更新日期:1993-01-01 00:00:00

  • Construction and characterization of a multilayered gingival keratinocyte culture model: the TURK-U model.

    abstract::In construction of epithelial cells as multilayers, the cells are grown submerged to confluence on fibroblast-embedded collagen gels and, then, lifted to air to promote their stratification. We recently demonstrated that gingival epithelial cells form uniform monolayers on semi-permeable nitrocellulose membranes, supp...


    pub_type: 杂志文章


    authors: Gursoy UK,Gursoy M,Könönen E,Sintim HO,Uitto VJ,Syrjänen S

    更新日期:2016-12-01 00:00:00

  • Inhibition of hepatoma cell invasion beneath mesothelial-cell monolayer by sera from tea- and related component-treated rats and their modes of action.

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    pub_type: 杂志文章


    authors: Zhang G,Miura Y,Yagasaki K

    更新日期:2001-07-01 00:00:00

  • A rat mammary gland cancer cell with stem cell properties of self-renewal and multi-lineage differentiation.

    abstract::The cancer stem cell hypothesis posits that tumors are derived from a single cancer-initiating cell with stem cell properties. The task of identifying and characterizing cancer-initiating cells with stem cell properties at the single cell level has proven technically difficult because of the scarcity of the cancer ste...


    pub_type: 杂志文章


    authors: Cocola C,Sanzone S,Astigiano S,Pelucchi P,Piscitelli E,Vilardo L,Barbieri O,Bertoli G,Reinbold RA,Zucchi I

    更新日期:2008-09-01 00:00:00