Production of high titre disabled infectious single cycle (DISC) HSV from a microcarrier culture.


:Disabled Infectious Single Cycle (DISC) HSV-2 has been cultured in the complimentary cell line CR2 to provide high titre bulk material suitable for the purification of the virus as a live viral vaccine. CR2 cells are cultured on the microcarrier Cytodex-1 at 5 g l-1 in small scale (1 l) and larger scale (15 l) reactors. The cells are infected at an MOI of 0.01 pfu cell-1 and the culture harvested 60-72 h later. The infected cells are removed from the microcarriers by the addition of a hypotonic saline and the virus released by low-pressure disruption techniques. Virus titres achieved are compared to the standard roller bottle process. The resulting material is the starting point for the purification of the DISC-HSV virus.






Zecchini TA,Smith RJ




Has Abstract


1999-07-01 00:00:00












  • Use of cell cultures, nuclear and radioanalytical techniques for metallotoxicological studies at the JRC-Ispra.

    abstract::This paper reviews the JRC-Ispra research activity on toxicological studies related to the environmental and occupational exposure of trace metals, as carried out by a combination of cell culture methodologies with nuclear and radioanalytical techniques. Applications concern the setting of uptake-effect relationships ...


    pub_type: 杂志文章


    authors: Sabbioni E,Pozzi G,Fischbach M,Bahbouth E

    更新日期:1993-01-01 00:00:00

  • Effect of Partial Medium Replacement on Cell Growth and Protein Production for the High-Fivetrade mark insect cell line.

    abstract::The potential of spent medium to support the growth and recombinant protein production of High-Fivetrade mark cells was investigated. Growth in medium consisting of three parts fresh and one part spent medium was comparable to that in fresh medium (maximal specific growth rates of 0.028 and 0.029 h(-1), and maximal ce...


    pub_type: 杂志文章


    authors: Ikonomou L,Bastin G,Schneider YJ,Agathos SN

    更新日期:2004-01-01 00:00:00

  • Development and characterization of insect cell lines.

    abstract:CONCLUSIONS:With the wide availability of insect cell culture media, it can generally be considered a routine process to develop new cell lines. Exceptions to this statement do exist, of course. Difficulties may arise when attempting to culture a specific cell type. For example, while there are a few cell lines from in...


    pub_type: 杂志文章


    authors: Lynn DE

    更新日期:1996-01-01 00:00:00

  • Efficient production of recombinant IgG by metabolic control and co-expression with GLUT5 in a fructose-based medium.

    abstract::A fructose-based cell culture is suitable for the process control of protein production because of slow sugar consumption rate and low lactate accumulation. The fructose transporter, GLUT5, mediates its incorporation into cells and is required for the fructose-based culture. In order to produce efficiently recombinant...


    pub_type: 杂志文章


    authors: Inoue Y,Tsukamoto Y,Yamanaka M,Nakamura S,Inoue A,Nishino N,Kawahara H

    更新日期:2010-08-01 00:00:00

  • Effect of some constituents of chicken egg yolk lipoprotein on the growth and IgM production of human-human hybridoma cells and other human-derived cells.

    abstract::Chicken egg yolk lipoprotein (YLP) was partially fractionated into some constituents, and the effect of constituents of YLP were examined on the growth and immunoglobulin (IgM and IgG) secretion of a HB4C5 human-human hybridoma cell line cultured in serum-free medium. Among the fractions, YP-1 and YP-2 fractions (LDL-...


    pub_type: 杂志文章


    authors: Shinohara K,Fukushima T,Suzuki M,Tsutsumi M,Kobori M,Kong ZL

    更新日期:1993-01-01 00:00:00

  • A two-compartment cell entrapment bioreactor with three different holding times for cells, high and low molecular weight compounds.

    abstract::A new bioreactor for animal cell cultivation employs two compartments for cells and medium respectively. The two chambers are separated by an ultrafiltration membrane. Cells and solution of collagen or collagen/chitosan mixture were loaded to the cell chamber and were allowed to form gel inside. Contraction of the cel...


    pub_type: 杂志文章


    authors: Scholz M,Hu WS

    更新日期:1990-09-01 00:00:00

  • Cytogenetic effects of nine Helichrysum taxa in human lymphocytes culture.

    abstract::Helichrysum Mill. (Asteraceae) species have been used in folk medicine for thousands of years in the world. The in vitro cytogenetic effects in human lymphocytes of nine Helichrysum taxa used in Turkey folk medicine were investigated. Blood samples were obtained from healthy donors, non-smoking volunteers, which were ...


    pub_type: 杂志文章


    authors: Eroğlu HE,Aksoy A,Hamzaoğlu E,Budak U,Albayrak S

    更新日期:2009-01-01 00:00:00

  • Apoptosis resistance in tumor cells.

    abstract::Various antitumor agents induce apoptotic cell death in tumor cells. Since the apoptosis program in tumor cells plays a critical role in the chemotherapy-induced tumor cell killing, it is suggested that the defect in the signaling pathway of apoptosis could cause a new form of multidrug resistance in tumor cells. This...


    pub_type: 杂志文章


    authors: Mashima T,Seimiya H,Chen Z,Kataoka S,Tsuruo T

    更新日期:1998-09-01 00:00:00

  • Analysis of toxicity effects of Di-(2-ethylhexyl) phthalate exposure on human bronchial epithelial 16HBE cells.

    abstract::Recent studies have indicated that Di-(2-ethylhexyl) phthalate (DEHP), the most commonly used plasticizer in daily-life products, could be dispersed in indoor air and induce human exposure via inhalation. DEHP has been reported to have effects on the respiratory system in both animal and human researches. The toxicity...


    pub_type: 杂志文章


    authors: Ma Y,Guo Y,Wu S,Lv Z,Zhang Q,Xie X,Ke Y

    更新日期:2018-02-01 00:00:00

  • Effects of medium-chain fatty acids on the structure and immune response of IPEC-J2 cells.

    abstract::Medium-chain fatty acids (MCFAs) have been suggested as an alternative to the use of antibiotics in animal nutrition with promising results. First, we studied the sensitivity of Salmonella Enteritidis and an enteropathogenic Escherichia coli strain against caprylic (C8), capric (C10) and lauric (C12) acids. A porcine ...


    pub_type: 杂志文章


    authors: Martínez-Vallespín B,Vahjen W,Zentek J

    更新日期:2016-10-01 00:00:00

  • Fibroblast growth factor complexed to the cytotoxin saporin is growth inhibitory but not cytotoxic for embryonal carcinoma cells.

    abstract::Fibroblast growth factors (FGFs) have been implicated in a number of proliferative lesions, including malignant tumor growth and vascularization. As a result, cytotoxic agents that target cell surface FGF receptors are currently under investigation. Previous reports have shown that conjugation of basic FGF with the ri...


    pub_type: 杂志文章


    authors: Miller K,Wilder PJ,Rizzino A

    更新日期:1993-01-01 00:00:00

  • Highly passage of Spodoptera litura cell line causes its permissiveness to baculovirus infection.

    abstract::It is well known that the characteristics of cell lines possibly alter when cell lines are at high-passage number because of the environmental selection. We do not know whether non-permissive or low-permissive cell lines could become permissive or more permissive to virus infection after over-high passage. In the pres...


    pub_type: 杂志文章


    authors: Zhang X,Lan W,Deng Y,Ma Y,Liu K,Peng J,Li Y,Hong H

    更新日期:2008-07-01 00:00:00

  • On-line heat flux measurements improve the culture medium for the growth and productivity of genetically engineered CHO cells.

    abstract::With the increasingly competitive commercial production of target proteins by hybridoma and genetically engineered cells, there is an urgent requirement for biosensors to monitor and control on-line and in real time the growth of cultured cells. Since growth is accompanied by an enthalpy change, heat dissipation measu...


    pub_type: 杂志文章


    authors: Guan YH,Kemp RB

    更新日期:1999-07-01 00:00:00

  • Versatile stem cells, young and old. A review.

    abstract::Both embryonic and somatic stem cells have been studied in recent years with particular regard to their differentiation potential. In vitro studies allow a considerable amplification of such cells in culture as well as the induction of commitment in different directions under proper stimulating factors. Moreover, a su...


    pub_type: 杂志文章


    authors: Eridani S

    更新日期:2001-03-01 00:00:00

  • Liver stem cells.

    abstract::The capacity of hepatocytes and cholangiocytes to contribute to their own maintenance has long been recognized. More recently, studies have indicated the presence of both intra-hepatic and extra-hepatic stem/progenitor cell populations. The intraorgan compartment probably derives primarily from the biliary tree, most ...


    pub_type: 杂志文章


    authors: Theise ND

    更新日期:2003-03-01 00:00:00

  • Structure-activity relationship of antioxidants for inhibitors of linoleic acid hydroperoxide-induced toxicity in cultured human umbilical vein endothelial cells.

    abstract::Structure-activity relationship of antioxidants for the protective effects on linoleic acid hydroperoxide (LOOH)-induced toxicity were examined in cultured human umbilical vein endothelial cells. alpha-Tocopherol, 2,2,5,7,8-pentamethylchroman-6-ol, butylated hydroxytoluene, probucol, and fatty acid esters of ascorbic ...


    pub_type: 杂志文章


    authors: Kaneko T,Baba N,Matsuo M

    更新日期:2001-01-01 00:00:00

  • Aldehyde dehydrogenase activity of Wharton jelly mesenchymal stromal cells: isolation and characterization.

    abstract::Mesenchymal stromal cells (MSCs) are promising tools in regenerative medicine and targeted therapies. Although different origins have been described, there is still huge need to find a valuable source harboring specific subpopulations of MSCs with precise therapeutic functions. Here, we isolated by fluorescence activa...


    pub_type: 杂志文章


    authors: Najar M,Crompot E,van Grunsven LA,Dollé L,Lagneaux L

    更新日期:2019-02-01 00:00:00

  • Automated time-lapse microscopy and high-resolution tracking of cell migration.

    abstract::We describe a novel fully automated high-throughput time-lapse microscopy system and evaluate its performance for precisely tracking the motility of several glioma and osteoblastic cell lines. Use of this system revealed cell motility behavior not discernable with conventional techniques by collecting data (1) from cl...


    pub_type: 杂志文章


    authors: Fotos JS,Patel VP,Karin NJ,Temburni MK,Koh JT,Galileo DS

    更新日期:2006-05-01 00:00:00

  • Human fetal aorta-derived vascular progenitor cells: identification and potential application in ischemic diseases.

    abstract::Vasculogenesis, the formation of blood vessels in embryonic or fetal tissue mediated by immature vascular cells (i.e., angioblasts), is poorly understood. Here we report a summary of our recent studies on the identification of a population of vascular progenitor cells (VPCs) in human fetal aorta. These undifferentiate...


    pub_type: 杂志文章


    authors: Invernici G,Madeddu P,Emanueli C,Parati EA,Alessandri G

    更新日期:2008-09-01 00:00:00

  • Anin vitro model for the biological evaluation of dental materials using human periodontal ligament cells.

    abstract::Anin vitro system is described which uses human diploid cells derived from the periodontal ligament. It simulates the clinical situation and is suitable for rapid cytotoxicity screening of dental materials. It can also be adapted to study various factors influencing biocompatibility. ...


    pub_type: 杂志文章


    authors: Adams AM,Soames JV,Searle RF

    更新日期:1988-02-01 00:00:00

  • The effect of glucose and glutamine on the intracellular nucleotide pool and oxygen uptake rate of a murine hybridoma.

    abstract::The effects of media concentrations of glucose andglutamine on the intracellular nucleotide pools andoxygen uptake rates of a murine antibody-secretinghybridoma cell line were investigated. Cells takenfrom mid-exponential phase of growth were incubated inmedium containing varying concentrations of glucose(0-25 mM) and...


    pub_type: 杂志文章


    authors: Barnabé N,Butler M

    更新日期:2000-10-01 00:00:00

  • The organotypic culture of human skin keratinocytes and fibroblasts to achieve form and function.

    abstract::We describe an organotypic model of human skin comprised of a stratified layer of human epidermal keratinocytes and dermal fibroblasts within a contracted collagen lattice. Feasible and reproducible production of the skin construct has required the use of traditional as well as specialized culture techniques. The conf...


    pub_type: 杂志文章


    authors: Parenteau NL,Bilbo P,Nolte CJ,Mason VS,Rosenberg M

    更新日期:1992-01-01 00:00:00

  • Signs of deferasirox genotoxicity.

    abstract::Iron overload is a major health problem for patients who have to have continuous blood transfusions. It brings some metabolic problems together. Various iron chelating agents are being used for treatment of hemochromatosis which arises from excess iron accumulation. This study was conducted with the aim of determining...


    pub_type: 杂志文章


    authors: Ila HB,Topaktas M,Arslan M,Büyükleyla M

    更新日期:2014-08-01 00:00:00

  • Eicosapentaenoic acid protects against 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced hepatic toxicity in cultured rat hepatocytes.

    abstract::2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a persistent and ubiquitous environmental contaminant. The health impact of TCDD exposure is of great concern to the general public. Recent reports have implied that eicosapentaenoic acid (EPA) might be a potential chemopreventive agent and influence hepatotoxicity. The ai...


    pub_type: 杂志文章


    authors: Turkez H,Geyikoglu F,Mokhtar YI,Togar B

    更新日期:2012-01-01 00:00:00

  • Formation of monoclonal antibody against a major ginseng component, ginsenoside Rb1 and its characterization.

    abstract::The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rb1 was produced by fusing splenocytes immunized with a ginsenoside Rb1- bovine serum albumin conjugate wit...


    pub_type: 杂志文章


    authors: Tanaka H,Fukuda N,Shoyama Y

    更新日期:1999-03-01 00:00:00

  • Silver/silver chloride nanoparticles inhibit the proliferation of human glioblastoma cells.

    abstract::Glioblastomas (GBM) are aggressive brain tumors with very poor prognosis. While silver nanoparticles represent a potential new strategy for anticancer therapy, the silver/silver chloride nanoparticles (Ag/AgCl-NPs) have microbicidal activity, but had not been tested against tumor cells. Here, we analyzed the effect of...


    pub_type: 杂志文章


    authors: Eugenio M,Campanati L,Müller N,Romão LF,de Souza J,Alves-Leon S,de Souza W,Sant'Anna C

    更新日期:2018-12-01 00:00:00

  • Non-fucosylated therapeutic antibodies: the next generation of therapeutic antibodies.

    abstract::Therapeutic antibody IgG1 has two N-linked oligosaccharide chains bound to the Fc region. The oligosaccharides are of the complex biantennary type, composed of a trimannosyl core structure with the presence or absence of core fucose, bisecting N-acetylglucosamine (GlcNAc), galactose, and terminal sialic acid, which gi...


    pub_type: 杂志文章


    authors: Mori K,Iida S,Yamane-Ohnuki N,Kanda Y,Kuni-Kamochi R,Nakano R,Imai-Nishiya H,Okazaki A,Shinkawa T,Natsume A,Niwa R,Shitara K,Satoh M

    更新日期:2007-12-01 00:00:00

  • Intracellular localisation studies of doxorubicin and Victoria Blue BO in EMT6-S and EMT6-R cells using confocal microscopy.

    abstract::The subcellular localisation of doxorubicin and Victoria Blue BO (VBBO) in a murine mammary tumour cell line EMT6-S, and the resistant sub-lineEMT6-R was studied, using confocal microscopy, in order to investigate their sites of action. In cells treated with doxorubicin (10 mu M) for 90 min, the pattern of intracellul...


    pub_type: 杂志文章


    authors: Burrow SM,Phoenix DA,Wainwright M,Tobin MJ

    更新日期:2002-05-01 00:00:00

  • Echinococcus granulosus protoscoleces promotes proliferation and invasion of hepatocellular carcinoma cells.

    abstract::There may exist a connection between Echinococcus granulosus infection and cancer development. Here, it is aimed to investigate specific effects of E. granulosus protoscoleces (PSCs) on the proliferation and invasion capacities of hepatocellular carcinoma (HCC) cells in vitro and ex vitro. HepG2 cells were cultured wi...


    pub_type: 杂志文章


    authors: Yasen A,Wang M,Ran B,Lv G,Aji T,Xiao H,Shao Y,Wen H

    更新日期:2021-02-01 00:00:00

  • The human vascular endothelial cell line HUV-EC-C harbors the integrated HHV-6B genome which remains stable in long term culture.

    abstract::Human herpes virus 6 (HHV-6) is a common human pathogen that is most often detected in hematopoietic cells. Although human cells harboring chromosomally integrated HHV-6 can be generated in vitro, the availability of such cell lines originating from in vivo tissues is limited. In this study, chromosomally integrated H...


    pub_type: 杂志文章


    authors: Shioda S,Kasai F,Ozawa M,Hirayama N,Satoh M,Kameoka Y,Watanabe K,Shimizu N,Tang H,Mori Y,Kohara A

    更新日期:2018-02-01 00:00:00