Production of high titre disabled infectious single cycle (DISC) HSV from a microcarrier culture.

Abstract:

:Disabled Infectious Single Cycle (DISC) HSV-2 has been cultured in the complimentary cell line CR2 to provide high titre bulk material suitable for the purification of the virus as a live viral vaccine. CR2 cells are cultured on the microcarrier Cytodex-1 at 5 g l-1 in small scale (1 l) and larger scale (15 l) reactors. The cells are infected at an MOI of 0.01 pfu cell-1 and the culture harvested 60-72 h later. The infected cells are removed from the microcarriers by the addition of a hypotonic saline and the virus released by low-pressure disruption techniques. Virus titres achieved are compared to the standard roller bottle process. The resulting material is the starting point for the purification of the DISC-HSV virus.

journal_name

Cytotechnology

journal_title

Cytotechnology

authors

Zecchini TA,Smith RJ

doi

10.1023/A:1008005200711

subject

Has Abstract

pub_date

1999-07-01 00:00:00

pages

203-10

issue

1-3

eissn

0920-9069

issn

1573-0778

journal_volume

30

pub_type

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