Antidiabetic effect of green rooibos (Aspalathus linearis) extract in cultured cells and type 2 diabetic model KK-A(y) mice.

Abstract:

:Previous studies have demonstrated antidiabetic effects for rooibos (Aspalathus linearis) and aspalathin (ASP), one of its main polyphenols. Rooibos, an endemic plant of South Africa, is well-known for its use as herbal tea. Green ('unfermented') rooibos has been shown to contain more ASP than 'fermented' rooibos tea, currently the major product. In the present study, we investigated the antidiabetic effect of green rooibos extract (GRE) through studies on glucose uptake in L6 myotubes and on pancreatic β-cell protective ability from reactive oxygen species (ROS) in RIN-5F cells. Its in vivo effect was also examined using obese diabetic KK-A(y) mice. GRE increased glucose uptake under insulin absent condition and induced phosphorylation of 5'-adenosine monophosphate-activated protein kinase (AMPK) in L6 myotubes as previously demonstrated for ASP. In addition to AMPK, GRE also promoted phosphorylation of Akt, another promoter of glucose transporter 4 (GLUT4) translocation, in L6 myotubes unlike ASP, suggesting an involvement of GRE component(s) other than ASP in Akt phosphorylation. Promotion of GLUT4 translocation to the plasma membrane by GRE in L6 myotubes was demonstrated by Western blotting analysis. GRE suppressed the advanced glycation end products (AGEs)-induced increase in ROS levels in RIN-5F pancreatic β-cells. Subchronic feeding with GRE suppressed the increase in fasting blood glucose levels in type 2 diabetic model KK-A(y) mice. These in vitro and in vivo results strongly suggest that GRE has antidiabetic potential through multiple modes of action.

journal_name

Cytotechnology

journal_title

Cytotechnology

authors

Kamakura R,Son MJ,de Beer D,Joubert E,Miura Y,Yagasaki K

doi

10.1007/s10616-014-9816-y

subject

Has Abstract

pub_date

2015-08-01 00:00:00

pages

699-710

issue

4

eissn

0920-9069

issn

1573-0778

journal_volume

67

pub_type

杂志文章
  • Development and characterization of insect cell lines.

    abstract:CONCLUSIONS:With the wide availability of insect cell culture media, it can generally be considered a routine process to develop new cell lines. Exceptions to this statement do exist, of course. Difficulties may arise when attempting to culture a specific cell type. For example, while there are a few cell lines from in...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00350384

    authors: Lynn DE

    更新日期:1996-01-01 00:00:00

  • Effect of purine supplementation on the growth of salmonid cell lines in different mammalian sera.

    abstract::The Chinook salmon embryo cell line, CHSE-214, grew well in fetal bovine serum (FBS) but poorly in dialyzed (d) FBS. Purines restored most but not all growth-promoting activity to dFBS, which suggests that purines account for a large portion of the dialyzable fraction's growth-promoting activity. CHSE-214 died in newb...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02540026

    authors: Ganassin RC,Bols NC

    更新日期:1992-01-01 00:00:00

  • Chaperone and foldase coexpression in the baculovirus-insect cell expression system.

    abstract:CONCLUSIONS:The BEVS has become widely utilized for production of recombinant proteins. However, protein aggregation and inefficient processing often limit yields, especially for secreted and membrane proteins. Since many proteins of pharmaceutical interest require similar posttranslational processing steps, engineerin...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00350396

    authors: Betenbaugh MJ,Ailor E,Whiteley E,Hinderliter P,Hsu TA

    更新日期:1996-01-01 00:00:00

  • Are we prepared for animal cell technology in the 21st century?

    abstract::Large scale animal cell culture for the production of complex therapeutic proteins has been a major success of the biotechnology industry. Today, approximately half of the $ 5 billion annual turnover of the biotechnology industry is based upon this technology, in many cases with reactors of more than 10 m(3). As we lo...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00744313

    authors: Cooney CL

    更新日期:1995-01-01 00:00:00

  • Echinococcus granulosus protoscoleces promotes proliferation and invasion of hepatocellular carcinoma cells.

    abstract::There may exist a connection between Echinococcus granulosus infection and cancer development. Here, it is aimed to investigate specific effects of E. granulosus protoscoleces (PSCs) on the proliferation and invasion capacities of hepatocellular carcinoma (HCC) cells in vitro and ex vitro. HepG2 cells were cultured wi...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-020-00437-0

    authors: Yasen A,Wang M,Ran B,Lv G,Aji T,Xiao H,Shao Y,Wen H

    更新日期:2021-02-01 00:00:00

  • Immunocytochemistry assay in BHK-21 cell line infected with Porcine Sapelovirus.

    abstract::The present study describes an immunocytochemistry (ICC) assay with self-raised hyperimmune sera and a Baby Hamster Kidney-21 (BHK-21) cell line infected with Porcine Sapelovirus (PSV). Sapelovivus/IVRI/SPF-c-6/2015 strain Indian PSV was isolated from the porcine IBRS-2 cell line and investigated for growth on non-por...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-019-00315-4

    authors: Kumari S,Singh R,Desingu PA,Ray PK,Taru Sharma G,Saikumar G

    更新日期:2019-06-01 00:00:00

  • Effects of temperature shift on cell cycle, apoptosis and nucleotide pools in CHO cell batch cultues.

    abstract::Temperature reduction in CHO cell batch culture may be beneficial in the production of recombinant protein and in maintenance of viability. The effects on cell cycle, apoptosis and nucleotide pools were studied in cultures initiated at 37°C and temperature shifted to 30 °C after 48 hours. In control cultures maintaine...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1023/A:1007919921991

    authors: Moore A,Mercer J,Dutina G,Donahue CJ,Bauer KD,Mather JP,Etcheverry T,Ryll T

    更新日期:1997-01-01 00:00:00

  • Interferon-γ-induced differentiation of human neuroblastoma cells increases cellular uptake and halflife of metaiodobenzylguanidine.

    abstract::Iodine labeled metaiodobenzylguanidine (MIBG) is a radiopharmaceutical employed for both diagnosis and metabolic radiotherapy of neuroblastoma (NB). Resistance to the radiotherapeutic effects of MIBG is common, due to lack of MIBG accumulation by NB cells. MIBG enters competent cells via the noradrenaline transporter;...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00746080

    authors: Montaldo PG,Carbone R,Cornaglia Ferraris P,Ponzoni M

    更新日期:1993-01-01 00:00:00

  • Heterohybridomas that secrete high levels of pseudomonas-specific therapeutic human monoclonal antibodies: their generation and large scale growth in an automated hollow fiber cell culture system.

    abstract::Fusion of lymphoblastoid cell lines that produce human monoclonal antibodies against Pseudomonas aeruginosa with the human/mouse heteromyeloma SHM-D33 generated heterohybrids that were stable and secreted antibody in the range of 20 to 300 micrograms/ml. One of the hybridoma cell lines ws adapted to serum-free medium ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00365266

    authors: Gammon MC,Banas MH,Boccumini LE,Sigal NH,Zweerink HJ

    更新日期:1990-01-01 00:00:00

  • Engineering challenges in high density cell culture systems.

    abstract::High density cell culture systems offer the advantage of production of bio-pharmaceuticals in compact bioreactors with high volumetric production rates; however, these systems are difficult to design and operate. First of all, the cells have to be retained in the bioreactor by physical means during perfusion. The desi...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00353919

    authors: Ozturk SS

    更新日期:1996-01-01 00:00:00

  • Effects of medium-chain fatty acids on the structure and immune response of IPEC-J2 cells.

    abstract::Medium-chain fatty acids (MCFAs) have been suggested as an alternative to the use of antibiotics in animal nutrition with promising results. First, we studied the sensitivity of Salmonella Enteritidis and an enteropathogenic Escherichia coli strain against caprylic (C8), capric (C10) and lauric (C12) acids. A porcine ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-016-0003-1

    authors: Martínez-Vallespín B,Vahjen W,Zentek J

    更新日期:2016-10-01 00:00:00

  • Construction and characterization of a multilayered gingival keratinocyte culture model: the TURK-U model.

    abstract::In construction of epithelial cells as multilayers, the cells are grown submerged to confluence on fibroblast-embedded collagen gels and, then, lifted to air to promote their stratification. We recently demonstrated that gingival epithelial cells form uniform monolayers on semi-permeable nitrocellulose membranes, supp...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-016-0029-4

    authors: Gursoy UK,Gursoy M,Könönen E,Sintim HO,Uitto VJ,Syrjänen S

    更新日期:2016-12-01 00:00:00

  • Genotoxicity of Thermopsis turcica on Allium cepa L. roots revealed by alkaline comet and random amplified polymorphic DNA assays.

    abstract::This study was undertaken to evaluate genotoxic potential of Thermopsis turcica aqueous extracts on the roots of onion bulb (Allium cepa L.) by comet assay and random amplified polymorphic DNA technique. The Allium root growth inhibition test indicated that the EC50 and 2×EC50 values were 8 and 16 mg/ml concentrations...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-014-9835-8

    authors: Ciğerci İH,Cenkci S,Kargıoğlu M,Konuk M

    更新日期:2016-08-01 00:00:00

  • The human vascular endothelial cell line HUV-EC-C harbors the integrated HHV-6B genome which remains stable in long term culture.

    abstract::Human herpes virus 6 (HHV-6) is a common human pathogen that is most often detected in hematopoietic cells. Although human cells harboring chromosomally integrated HHV-6 can be generated in vitro, the availability of such cell lines originating from in vivo tissues is limited. In this study, chromosomally integrated H...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-017-0119-y

    authors: Shioda S,Kasai F,Ozawa M,Hirayama N,Satoh M,Kameoka Y,Watanabe K,Shimizu N,Tang H,Mori Y,Kohara A

    更新日期:2018-02-01 00:00:00

  • Expression of recombinant Atlantic salmon serum C-type lectin in Drosophila melanogaster Schneider 2 cells.

    abstract::The Atlantic salmon (Salmo salar) serum lectin (SSL) is a soluble C-type lectin that binds bacteria, including salmon pathogens. This lectin is a cysteine-rich oligomeric protein. Consequently, a Drosophila melanogaster expression system was evaluated for use in expressing SSL. A cDNA encoding SSL was cloned into a ve...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-012-9505-7

    authors: Uribe E,Venkatesan M,Rose DR,Ewart KV

    更新日期:2013-08-01 00:00:00

  • Modeling of cell culture processes.

    abstract::Models of cell processes can be particularly useful in simulating, optimizing and controlling cell culture systems. Models reported in the literature are of various degrees of biological structure and mathematical complexity and describe cell growth, death, metabolism, and product formation, alone or in combination wi...

    journal_title:Cytotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/BF00749616

    authors: Tziampazis E,Sambanis A

    更新日期:1994-01-01 00:00:00

  • Estimation of rates of oxygen uptake and carbon dioxide evolution of animal cell culture using material and energy balances.

    abstract::Material and degree of reductance balance equations are used to estimate the rates of oxygen uptake and carbon dioxide evolution of animal cell cultures. Lumped compositions, molecular weight and reductance degree of cellular protein, monoclonal antibody, biomass and amino acid consumption (excluding glutamine and ala...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1023/A:1008004618163

    authors: Xiu ZL,Deckwer WD,Zeng AP

    更新日期:1999-05-01 00:00:00

  • The cell yields and biological characteristics of stromal/stem cells from lipoaspirate with different digestion loading ratio.

    abstract::Effective harvesting procedure for adipose tissue is demanded by the affordable Good Manufacturing Practice-Compliant Production of clinical-grade adipose tissue-derived stem cells (hADSCs). Enzymatic digestion using collagenase is the most reliable method of adipose tissue-derived stem cells (hADSCs) isolation, while...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-020-00369-9

    authors: Li Z,Mu D,Liu C,Xin M,Fu S,Li S,Qi J,Wang Q,Luan J

    更新日期:2020-04-01 00:00:00

  • Molecular mechanisms of Bombyx batryticatus ethanol extract inducing gastric cancer SGC-7901 cells apoptosis.

    abstract::Bombyx batryticatus is a traditional Chinese medicine. To understand apoptotic effect of B. batryticatus ethanol extract (BBE), we investigated the role of BBE in inducing apoptosis of human gastric cancer cells SGC-7901. Cells treated with BBE and apoptosis was assessed by methyl thiazolyl tetrazolium (MTT) assay, mo...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-017-0102-7

    authors: Wu JY,Sheikho A,Ma H,Li TC,Zhao YQ,Zhang YL,Wang D

    更新日期:2017-12-01 00:00:00

  • Can macroscopic identification of large ovarian follicles be useful in eliminating follicles with impaired steroid function?

    abstract::"In vitro" studies, commonly using porcine ovarian follicles, may generate inconsistent results when atretic follicles are not eliminated from the pool of experimental follicles. The present experiment was conducted to test the practical value of the macroscopie identification of large porcine follicles, which were as...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00758179

    authors: Dudek D

    更新日期:1994-01-01 00:00:00

  • The insulin-like growth factor II/mannose-6-phosphate receptor : IGF-II/Man-6-P receptor.

    abstract::Recent evidence from molecular cloning, biochemical and immunological experiments has established that the cation-independent mannose-6-phosphate (Man-6-P) receptor and insulin-like growth factor-II (IGF-II) receptor are the same protein. Although the role of the IGF-II/Man-6-P receptor as a transporter of hydrolytic ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00364994

    authors: Macdonald RG

    更新日期:1989-12-01 00:00:00

  • Hypoxia enhances proliferation and stemness of human adipose-derived mesenchymal stem cells.

    abstract::The aim of the study was to obtain the highest number of multipotent adipose-derived mesenchymal stem cells (ADMSCs) by using culture conditions which favour cell expansion without loss of mesenchymal stem cells (MSC)-like properties. Based on the assumption that stem cells reside in niches characterized by hypoxic co...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-014-9731-2

    authors: Fotia C,Massa A,Boriani F,Baldini N,Granchi D

    更新日期:2015-12-01 00:00:00

  • Evaluation of electroporation-induced adverse effects on adipose-derived stem cell exosomes.

    abstract::In the recent years, the possibility of utilizing extracellular vesicles for drug delivery purposes has been investigated in various models, suggesting that these vesicles may have such potential. In addition to the choice of donor cell type for vesicle production, a major obstacle still exists with respect of loading...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-016-9952-7

    authors: Johnsen KB,Gudbergsson JM,Skov MN,Christiansen G,Gurevich L,Moos T,Duroux M

    更新日期:2016-10-01 00:00:00

  • Antibodies in the study of multiple drug resistance.

    abstract::Multidrug resistance (MDR) is a major problem in cancer chemotherapy. As P-glycoprotein is the key molecule in MDR, many investigators have constructed anti-P-glycoprotein monoclonal antibodies (MAbs). Those antibodies, including MRK16 and C219, were used for elucidation of the mechanism of MDR and for overcoming of M...

    journal_title:Cytotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/BF00744659

    authors: Heike Y,Tsuruo T

    更新日期:1993-01-01 00:00:00

  • Expression of anchorin CII mRNA by cultured chondrocytes.

    abstract::The establishment of a cell culture system promoting chondrocyte differentiation has been utilized to better characterize phenotypic stages of chondrogenesis at the cellular level. Although the expression of the type II collagen gene has been studied during "in vitro" chondrocyte differentiation, little is known about...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00736804

    authors: Castagnola P,Cancedda R

    更新日期:1991-02-01 00:00:00

  • Molecular analysis of cross-reactive human monoclonal antibody AE6F4 generated by in vitro immunization: Epitope mapping of AE6F4 antibody on 14-3-3 family proteins and cytokeratin 8.

    abstract::We reported previously that adenocarcinoma-reactive human monoclonal antibody AE6F4, which had been generated by in vitro immunization method, recognizes both 14-3-3protein and cytokeratin 8 (CK8). In this study, to analyze the cross-reactivity of AE6F4 antibody, epitopes of AE6F4 antibody on 14-3-3 proteins and CK8 w...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1023/A:1014060113186

    authors: Ichikawa A,Shim SY,Katakura Y,Teruya K,Hashizume S,Shirahata S

    更新日期:2001-07-01 00:00:00

  • Study of the cytotoxic activity of Styrax camporum extract and its chemical markers, egonol and homoegonol.

    abstract::The benzofuran lignans egonol and homoegonol are found in all species of the genus Styrax. Since natural products are important sources of new anticancer drugs, this study evaluated the cytotoxic activity of a hydroalcoholic extract of the stems of S. camporum (SCHE) and their chemical markers, egonol (EG) and homoego...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-015-9864-y

    authors: de Oliveira PF,Damasceno JL,Bertanha CS,Araújo AR,Pauletti PM,Tavares DC

    更新日期:2016-08-01 00:00:00

  • A luciferase complementation assay system using transferable mouse artificial chromosomes to monitor protein-protein interactions mediated by G protein-coupled receptors.

    abstract::G protein-coupled receptors (GPCRs) are seven-transmembrane domain receptors that interact with the β-arrestin family, particularly β-arrestin 1 (ARRB1). GPCRs interact with 33% of small molecule drugs. Ligand screening is promising for drug discovery concerning GPCR-related diseases. Luciferase complementation assay ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-018-0231-7

    authors: Uno N,Fujimoto T,Komoto S,Kurosawa G,Sawa M,Suzuki T,Kazuki Y,Oshimura M

    更新日期:2018-12-01 00:00:00

  • Role of a putative tyrosine-O-sulfate receptor in the targeting and/or intracellular transport of tyrosine-sulfated proteins.

    abstract::By employing the affinity gel fraction technique, we have detected a 175 kDa tyrosine-O-sulfate (TyrS)-binding protein in sodium choleate extracts of the microsomal membrane fractions of bovine liver and pancreas, as well as canine liver and pancreas. Western blot analysis revealed the presence of the bovine liver Tyr...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1023/A:1007903315687

    authors: Liu MC,Sakakibara Y,Suiko M

    更新日期:1997-01-01 00:00:00

  • The role of natural indigo dye in alleviation of genotoxicity of sodium dithionite as a reducing agent.

    abstract::Indigo blue is a natural dye used for thousands of years by civilizations to dye fabric blue and it is naturally obtained from Isatis tinctoria. I. tinctoria is not only used for extraction of indigo blue color but also used medicinally in Traditional Chinese Medicine because of its active compounds. Sodium dithionite...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-016-0018-7

    authors: Bektaş İ,Karaman Ş,Dıraz E,Çelik M

    更新日期:2016-12-01 00:00:00