Automated time-lapse microscopy and high-resolution tracking of cell migration.


:We describe a novel fully automated high-throughput time-lapse microscopy system and evaluate its performance for precisely tracking the motility of several glioma and osteoblastic cell lines. Use of this system revealed cell motility behavior not discernable with conventional techniques by collecting data (1) from closely spaced time points (minutes), (2) over long periods (hours to days), (3) from multiple areas of interest, (4) in parallel under several different experimental conditions. Quantitation of true individual and average cell velocity and path length was obtained with high spatial and temporal resolution in "scratch" or "wound healing" assays. This revealed unique motility dynamics of drug-treated and adhesion molecule-transfected cells and, thus, this is a considerable improvement over current methods of measurement and analysis. Several fluorescent vital labeling methods commonly used for end-point analyses (GFP expression, DiO lipophilic dye, and Qtracker nanocrystals) were found to be useful for time-lapse studies under specific conditions that are described. To illustrate one application, fluorescently labeled tumor cells were seeded onto cell monolayers expressing ectopic adhesion molecules, and this resulted in consistently reduced tumor cell migration velocities. These highly quantitative time-lapse analysis methods will promote the creation of new cell motility assays and increase the resolution and accuracy of existing assays.






Fotos JS,Patel VP,Karin NJ,Temburni MK,Koh JT,Galileo DS




Has Abstract


2006-05-01 00:00:00












  • On-line heat flux measurements improve the culture medium for the growth and productivity of genetically engineered CHO cells.

    abstract::With the increasingly competitive commercial production of target proteins by hybridoma and genetically engineered cells, there is an urgent requirement for biosensors to monitor and control on-line and in real time the growth of cultured cells. Since growth is accompanied by an enthalpy change, heat dissipation measu...


    pub_type: 杂志文章


    authors: Guan YH,Kemp RB

    更新日期:1999-07-01 00:00:00

  • Influence of aeration-homogenization system in stirred tank bioreactors, dissolved oxygen concentration and pH control mode on BHK-21 cell growth and metabolism.

    abstract::This work focused on determining the effect of dissolved oxygen concentration (DO) on growth and metabolism of BHK-21 cell line (host cell for recombinant proteins manufacturing and viral vaccines) cultured in two stirred tank bioreactors with different aeration-homogenization systems, as well as pH control mode. BHK-...


    pub_type: 杂志文章


    authors: Núñez EG,Leme J,de Almeida Parizotto L,Chagas WA,de Rezende AG,da Costa BL,Monteiro DC,Boldorini VL,Jorge SA,Astray RM,Pereira CA,Caricati CP,Tonso A

    更新日期:2014-08-01 00:00:00

  • Cephalic hedgehog expression is regulated directly by Sox17 in endoderm development of Xenopus laevis.

    abstract::In early development of animals, hedgehog (Hh) genes function as morphogen in the axis determination and the organ formation. In Xenopus, three hedgehog genes, sonic (shh), banded (bhh), and cephalic (chh), were identified and might organize various tissues and organs in embryogenesis. Here, we report the spatial and ...


    pub_type: 杂志文章


    authors: Yagi Y,Ito Y,Kuhara S,Tashiro K

    更新日期:2008-06-01 00:00:00

  • Evaluation of electroporation-induced adverse effects on adipose-derived stem cell exosomes.

    abstract::In the recent years, the possibility of utilizing extracellular vesicles for drug delivery purposes has been investigated in various models, suggesting that these vesicles may have such potential. In addition to the choice of donor cell type for vesicle production, a major obstacle still exists with respect of loading...


    pub_type: 杂志文章


    authors: Johnsen KB,Gudbergsson JM,Skov MN,Christiansen G,Gurevich L,Moos T,Duroux M

    更新日期:2016-10-01 00:00:00

  • Development of a Mammalian suspension culture for expression of active recombinant human urokinase-type plasminogen activator.

    abstract::The development of specific catalytic inhibitors for the serine protease urokinase-type plasminogen activator (uPA) has been hindered due to difficulties in producing sufficient amounts of active recombinant uPA that is catalytically equivalent to native uPA. The purpose of this study was to develop an efficient syste...


    pub_type: 杂志文章


    authors: Atkins E,Zamora S,Candia BJ,Baca A,Orlando RA

    更新日期:2005-09-01 00:00:00

  • Expression of anchorin CII mRNA by cultured chondrocytes.

    abstract::The establishment of a cell culture system promoting chondrocyte differentiation has been utilized to better characterize phenotypic stages of chondrogenesis at the cellular level. Although the expression of the type II collagen gene has been studied during "in vitro" chondrocyte differentiation, little is known about...


    pub_type: 杂志文章


    authors: Castagnola P,Cancedda R

    更新日期:1991-02-01 00:00:00

  • Anti-hyperuricemic effect of isorhamnetin in cultured hepatocytes and model mice: structure-activity relationships of methylquercetins as inhibitors of uric acid production.

    abstract::Hyperuricemia is an important risk factor for gout. Isorhamnetin (3'-O-methylquercetin) is an O-methylated flavonol, which occurs in onion, almond and sea buckthorn. It is also one of the metabolites of quercetin in mammals. In the present study, we investigated anti-hyperuricemic effect of isorhamnetin adopting both ...


    pub_type: 杂志文章


    authors: Adachi SI,Kondo S,Sato Y,Yoshizawa F,Yagasaki K

    更新日期:2019-02-01 00:00:00

  • A high-yielding serum-free, suspension cell culture process to manufacture recombinant adenoviral vectors for gene therapy.

    abstract::We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum free media and suspension growth. Subsequent...


    pub_type: 杂志文章


    authors: Schoofs G,Monica TJ,Ayala J,Horwitz J,Montgomery T,Roth G,Castillo FJ

    更新日期:1998-11-01 00:00:00

  • Inhibitory effects of chlorogenic acid and its related compounds on the invasion of hepatoma cells in culture.

    abstract::Actions of chlorogenic acid, a major component of coffee, andits constituents, caffeic and quinic acids, on theproliferation and invasion of AH109A, a rat ascites hepatomacell line, were investigated using in vitro assay systems. Allthree components suppressed the AH109A invasion atconcentrations of 5-40 muM without a...


    pub_type: 杂志文章


    authors: Yagasaki K,Miura Y,Okauchi R,Furuse T

    更新日期:2000-07-01 00:00:00

  • Anti-photoaging activities of Sorbaria kirilowii ethanol extract in UVB-damaged cells.

    abstract::Sorbaria kirilowii (Regel) Maxim, a plant found in China, Korea, Japan, and east of Europe, is a common herb used for traditional medicinal purposes. However, its ability to prevent photoaging has not been studied. In this study, we investigated the anti-photoaging functions of an ethanol extract (Sk-EE) of S. kirilow...


    pub_type: 杂志文章


    authors: Chen H,Jang J,Kopalli SR,Yum J,Yoon K,Cho JY

    更新日期:2021-02-01 00:00:00

  • Galangin induces B16F10 melanoma cell apoptosis via mitochondrial pathway and sustained activation of p38 MAPK.

    abstract::Galangin, an active flavonoid present at high concentration in Alpinia officinarum Hance and propolis, shows cytotoxicity towards several cancer cell lines, including melanoma. However, the specific cellular targets of galangin-induced cytotoxicity in melanoma are still unknown. Here, we investigated the effects of ga...


    pub_type: 杂志文章


    authors: Zhang W,Lan Y,Huang Q,Hua Z

    更新日期:2013-05-01 00:00:00

  • Role of a putative tyrosine-O-sulfate receptor in the targeting and/or intracellular transport of tyrosine-sulfated proteins.

    abstract::By employing the affinity gel fraction technique, we have detected a 175 kDa tyrosine-O-sulfate (TyrS)-binding protein in sodium choleate extracts of the microsomal membrane fractions of bovine liver and pancreas, as well as canine liver and pancreas. Western blot analysis revealed the presence of the bovine liver Tyr...


    pub_type: 杂志文章


    authors: Liu MC,Sakakibara Y,Suiko M

    更新日期:1997-01-01 00:00:00

  • Effect of some constituents of chicken egg yolk lipoprotein on the growth and IgM production of human-human hybridoma cells and other human-derived cells.

    abstract::Chicken egg yolk lipoprotein (YLP) was partially fractionated into some constituents, and the effect of constituents of YLP were examined on the growth and immunoglobulin (IgM and IgG) secretion of a HB4C5 human-human hybridoma cell line cultured in serum-free medium. Among the fractions, YP-1 and YP-2 fractions (LDL-...


    pub_type: 杂志文章


    authors: Shinohara K,Fukushima T,Suzuki M,Tsutsumi M,Kobori M,Kong ZL

    更新日期:1993-01-01 00:00:00

  • Interaction ofEscherichia coli cytotoxic necrotizing factor type 1 (CNF1) with cultured cells.

    abstract::The cytotoxic necrotizing factor type 1 (CNF1) fromE. coli causes necrosis in rabbit skin and multinucleation in cultured cells. Cells exposed to CNF1 were characterized by changes in actin organization, mainly consisting in the presence of well-developed stress fibers and membrane ruffles. The interaction of CNF1 wit...


    pub_type: 杂志文章


    authors: Falzano L,Fiorentini C,Boquet P,Donelli G

    更新日期:1993-01-01 00:00:00

  • Evaluation of monitoring approaches and effects of culture conditions on recombinant protein production in baculovirus-infected insect cells.

    abstract::The baculovirus infection process of Spodoptera frugiperda (Sf9) insect cells in oxygen-controlled bioreactors in serum-free medium was investigated using a recombinant Autographa californica (AcNPV) virus expressing beta-galactosidase enzyme as a model system. A variety of monitoring techniques including trypan blue ...


    pub_type: 杂志文章


    authors: Hensler WT,Agathos SN

    更新日期:1994-01-01 00:00:00

  • Rapid detection of cytotoxicity of food additives and contaminants by a novel cytotoxicity test, menadione-catalyzed H(2)O (2) production assay.

    abstract::Menadione-catalyzed H(2)O(2) production by viable animal cells was proportional to the viable cell number, and H(2)O(2) production decreased with increasing cytotoxic effects after the incubation of cells with cytotoxic compounds. The cytotoxic effects of food additives, pesticides, antibiotics, heavy metals, phytotox...


    pub_type: 杂志文章


    authors: Yamashoji S,Isshiki K

    更新日期:2001-11-01 00:00:00

  • High level expression of a frog alpha-amidating enzyme, AE-II, in cultured cells and silkworm larvae using a Bombyx mori nuclear polyhedrosis virus expression vector.

    abstract::A Xenopus laevis peptidyl C-terminal alpha-amidating enzyme (AE-II) gene, modified by deletion of a region encoding the putative membrane-spanning domain and the putative C-terminal cytosolic tail, was expressed in BoMo-15 AIIc insect cells and silkworm larvae using a Bombyx mori baculovirus expression vector system. ...


    pub_type: 杂志文章


    authors: Kobayashi J,Imanishi S,Inoue H,Ohsuye K,Yamaichi K,Tsuruoka N,Tanaka S

    更新日期:1992-01-01 00:00:00

  • Assessment of the in vitro cytotoxicity and in vivo anti-tumor activity of the alcoholic stem bark extract/fractions of Mimusops elengi Linn.

    abstract::Various parts of Mimusops elengi Linn. (Sapotaceae) have been used widely in traditional Indian medicine for the treatment of pain, inflammation and wounds. The study was conducted to explore the use of stem bark of M. elengi on pharmacological grounds and to evaluate the scientific basis of cytotoxic and anti-tumor a...


    pub_type: 杂志文章


    authors: Kumar H,Savaliya M,Biswas S,Nayak PG,Maliyakkal N,Manjunath Setty M,Gourishetti K,Pai KS

    更新日期:2016-08-01 00:00:00

  • Establishment of primary cell cultures from fish calcified tissues.

    abstract::Fishes have been recently recognized as a suitable model organism to study vertebrate physiological processes, in particular skeletal development and tissue mineralization. However, there is a lack of well characterized in vitro cell systems derived from fish calcified tissues. We describe here a protocol that was suc...


    pub_type: 杂志文章


    authors: Marques CL,Rafael MS,Cancela ML,Laizé V

    更新日期:2007-09-01 00:00:00

  • Development of a generic transient transfection process at 100 L scale.

    abstract::We have developed a generic transient transfection process at 100 L scale, using HEK293-EBNA cells and PEI as the transfection reagent for the production of recombinant IgG. The process, including large-scale plasmid preparation, expression at bioreactor scale, capture, purification and, if necessary, endotoxin remova...


    pub_type: 杂志文章


    authors: Tuvesson O,Uhe C,Rozkov A,Lüllau E

    更新日期:2008-02-01 00:00:00

  • Isolation, culture and identification of pulmonary arterial smooth muscle cells from rat distal pulmonary arteries.

    abstract::The culture of pulmonary arterial smooth muscle cells (PASMCs) is one of the most powerful tools for exploring the mechanisms of pulmonary hypertension (PH). Both pulmonary vasoconstriction and remodeling occur predominantly in distal pulmonary arteries (PA). In this study, we provide our detailed and standardized pro...


    pub_type: 杂志文章


    authors: Peng G,Xu J,Liu R,Fu Z,Li S,Hong W,Chen J,Li B,Ran P

    更新日期:2017-10-01 00:00:00

  • Generation of lymphokine activated killer cells in a new high density dialyzing culture apparatus.

    abstract::In order to establish an efficient culture system for the generation of lymphokine activated killer (LAK) cells, we have developed a new device which is essentially based on a continuous dialyzing culture vessel. LAK cells grown in such a system showed higher cytotoxicity than those grown under conventional culture co...


    pub_type: 杂志文章


    authors: Kohgo Y,Sakamaki S,Kanisawa Y,Nojiri S,Ueno Y,Itoh Y,Takahashi M,Sasagawa Y,Hosoi S,Sato S,Niitsu Y

    更新日期:1989-02-01 00:00:00

  • Evaluating the bottlenecks of recombinant IgM production in mammalian cells.

    abstract::Despite the fact, that monoclonal antibodies are the fastest growing group of biopharmaceuticals in development, this is not true for the IgM class, which remains as enigmatic as ever. While more examples of usefulness of IgMs for medical applications are emerging, their recombinant production is still not common. In ...


    pub_type: 杂志文章


    authors: Chromikova V,Mader A,Steinfellner W,Kunert R

    更新日期:2015-03-01 00:00:00

  • Cetyltriethylammonium bromide stimulating transcription of Bombyx mori nucleopolyhedrovirus gp64 gene promoter mediated by viral factors.

    abstract::To characterize the effects of cetyltriethylammonium bromide (CTAB) on the transcription of gp64 promoter from Bombyx mori nucleopolyhedrovirus (BmNPV), the plasmid pBmgp64Luc used in transient expression assay system was constructed by using the luciferase gene as a reporter under the control of BmNPV gp64 promoter. ...


    pub_type: 杂志文章


    authors: Zhou Y,Yi Y,Zhang Z,He J,Zhang Y

    更新日期:2003-01-01 00:00:00

  • MicroRNAs: recently discovered key regulators of proliferation and apoptosis in animal cells : Identification of miRNAs regulating growth and survival.

    abstract::The relatively recent discovery of miRNAs has added a completely new dimension to the study of the regulation of gene expression. The mechanism of action of miRNAs, the conservation between diverse species and the fact that each miRNA can regulate a number of targets and phenotypes clearly indicates the importance of ...


    pub_type: 杂志文章


    authors: Gammell P

    更新日期:2007-04-01 00:00:00

  • Genetic engineering of α2,6-sialyltransferase in recombinant CHO cells and its effects on the sialylation of recombinant interferon-γ.

    abstract::The CHO cell line has achieved considerable commercial importance as a vehicle for the production of human therapeutic proteins, but is known to lack a functional copy of the gene coding for α2,6-sialyltransferase (EC The cDNA for rat α2,6-ST was expressed in a recombinant CHO cell line making interferon-γ,...


    pub_type: 杂志文章


    authors: Monaco L,Marc A,Eon-Duval A,Acerbis G,Distefano G,Lamotte D,Engasser JM,Soria M,Jenkins N

    更新日期:1996-01-01 00:00:00

  • Modified CelliGen-packed bed bioreactors for hybridoma cell cultures.

    abstract::This study describes two packed bed bioreactor configurations which were used to culture a mouse-mouse hybridoma cell line (ATCC HB-57) which produces an IgG1 monoclonal antibody. The first configuration consists of a packed column which is continuously perfused by recirculating oxygenated media through the column. In...


    pub_type: 杂志文章


    authors: Wang G,Zhang W,Jacklin C,Freedman D,Eppstein L,Kadouri A

    更新日期:1992-01-01 00:00:00

  • Non-fucosylated therapeutic antibodies: the next generation of therapeutic antibodies.

    abstract::Therapeutic antibody IgG1 has two N-linked oligosaccharide chains bound to the Fc region. The oligosaccharides are of the complex biantennary type, composed of a trimannosyl core structure with the presence or absence of core fucose, bisecting N-acetylglucosamine (GlcNAc), galactose, and terminal sialic acid, which gi...


    pub_type: 杂志文章


    authors: Mori K,Iida S,Yamane-Ohnuki N,Kanda Y,Kuni-Kamochi R,Nakano R,Imai-Nishiya H,Okazaki A,Shinkawa T,Natsume A,Niwa R,Shitara K,Satoh M

    更新日期:2007-12-01 00:00:00

  • The transient expression of CHIKV VLP in large stirred tank bioreactors.

    abstract::Transient gene expression (TGE) bioprocesses have been difficult to scale up in large stirred tank bioreactors with volumes of more than 1.5 L. Low production levels are often observed, but the causes have not been investigated (Gutierrez-Granados et al. in Crit Rev Biotechnol 38:918-940, 2018). Chikungunya Virus-like...


    pub_type: 杂志文章


    authors: Chen P,Demirji J,Ivleva VB,Horwitz J,Schwartz R,Arnold F

    更新日期:2019-12-01 00:00:00

  • Genotypic and phenotypic changes of BHK-21 cells grown in suspension cultures.

    abstract::The propagation of foot-and-mouth disease virus on BHK-21 suspension cells, although economically convenient, may yield a scarcely immunizing antigen. Helpful insights were obtained by investigating a few genotypic and phenotypic features of the cell cultures. The appearance of polyploid populations, higher cell conce...


    pub_type: 杂志文章


    authors: Amadori M,Berneri C

    更新日期:1993-01-01 00:00:00