Abstract:
:The statistics of estimators used with the endpoint assay for virus titration were investigated. For a standard assay with 10 wells/dilution, the graphical estimator traditionally used was found to produce estimates with significant positive bias and a relatively low accuracy. Furthermore, the graphical estimator was found to be inconsistent. A superior estimator based on the maximum likelihood principle was developed. The results are discussed in relation to the choice between the endpoint titration assay and the plaque assay, and an alternative two-stage assay is presented.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Nielsen LK,Smyth GK,Greenfield PFdoi
10.1007/BF02522040subject
Has Abstractpub_date
1992-01-01 00:00:00pages
231-6issue
3eissn
0920-9069issn
1573-0778journal_volume
8pub_type
杂志文章相关文献
CYTOTECHNOLOGY文献大全abstract::Mandibular molar anlages excised from 17-day mouse foetuses were cultured in vitro or in ovo (on the chorioallantoic membrane). In both cases, the explants were underlain either with a Millipore filter or with a piece of fibrin foam. Tooth germs were harvested after 7 days of cultivation and processed histologically. ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00135638
更新日期:1991-09-01 00:00:00
abstract::Hepatic stellate cells (HSCs) are key players in liver fibrosis and regeneration via collagen degradation and synthesis. These phenomena involve inflammatory cytokines released from non-parenchymal liver cells such as Kupffer cells. Although the effects of individual cytokines on many cell types have been investigated...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-016-9948-3
更新日期:2017-06-01 00:00:00
abstract::Increasing interest has been observed in the use of photobiomodulation (PBM) to enhance the proliferation of stem cells and induce their differentiation. The effects of PBM at two different wavelengths (635 and 809 nm) with three different energy densities (0.5, 1 and 2 J/cm2) on the osteogenic differentiation of adip...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-020-00374-y
更新日期:2020-04-01 00:00:00
abstract::The use of the gadd153promoter to induce expression of a reporter geneunder heat stress conditions was investigated,since the results of previous studies have suggestedthat the gadd153promoter is likely to be activated by the indirecteffects of hyperthermia, that is, by DNA damage thatoccurs when reactive oxygen speci...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008181600197
更新日期:2000-07-01 00:00:00
abstract::Oral tolerance is the systemic immunological unresponsiveness that occurs after feeding protein antigens. Its physiological role is thought to be the prevention of hypersensitivity to food antigens, and its therapeutic use to treat inflammatory diseases has been suggested. Although it has been shown that CD4(+) T cell...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/b:cyto.0000039918.80472.0e
更新日期:2003-11-01 00:00:00
abstract::Desorption electrospray ionization may be used as a fast and convenient method for analysis and identification of lipids in the cell culture. Oxidative stress, which usually involves changes in lipids, was used as a model of pathology to show the utility of this analysis methodology. This paper addresses the surface p...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-014-9734-z
更新日期:2015-12-01 00:00:00
abstract::For the mouse hybridoma cell line VO 208, kinetics of growth, consumption of glucose and glutamine, and production of lactate, ammonia and antibodies were compared in batch and continuous cultures. At a given specific growth rate, different metabolic activities were observed: a 40% lower glucose and glutamine consumpt...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00570891
更新日期:1992-01-01 00:00:00
abstract::This paper reviews the JRC-Ispra research activity on toxicological studies related to the environmental and occupational exposure of trace metals, as carried out by a combination of cell culture methodologies with nuclear and radioanalytical techniques. Applications concern the setting of uptake-effect relationships ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::Mitochondrial dysfunction has been identified as one of the primary factors contributing to liver diseases. Pathways that control mitochondrial biogenesis are potential therapeutic targets for the amelioration of hepatocyte dysfunction and liver disease. Research on natural pharmacological agents that ameliorate liver...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-018-0242-4
更新日期:2018-12-01 00:00:00
abstract::The benzofuran lignans egonol and homoegonol are found in all species of the genus Styrax. Since natural products are important sources of new anticancer drugs, this study evaluated the cytotoxic activity of a hydroalcoholic extract of the stems of S. camporum (SCHE) and their chemical markers, egonol (EG) and homoego...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-015-9864-y
更新日期:2016-08-01 00:00:00
abstract::Therapeutic antibody IgG1 has two N-linked oligosaccharide chains bound to the Fc region. The oligosaccharides are of the complex biantennary type, composed of a trimannosyl core structure with the presence or absence of core fucose, bisecting N-acetylglucosamine (GlcNAc), galactose, and terminal sialic acid, which gi...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-007-9103-2
更新日期:2007-12-01 00:00:00
abstract::Human skin has continuous self-renewal potential throughout adult life and serves as first line of defence. Its cellular components such as human epidermal keratinocytes (HEKs) and dermal mesenchymal stromal cells (DMSCs) are valuable resources for wound healing applications and cell based therapies. Here we show a si...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-015-9857-x
更新日期:2016-08-01 00:00:00
abstract::Dioxacarb (Elecron, Famid) is a phenyl methylcarbamate insecticide and in vitro cytotoxic and genotoxic effects of this pesticide on human peripheral blood lymphocytes and Allium root meristematic cells were investigated by chromosomal aberrations (CAs) and Allium test. Human lymphocytes were treated with 62.5, 125, 2...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-014-9741-0
更新日期:2015-12-01 00:00:00
abstract::Sufentanil, a lipophilic opioid, is the most frequently used clinical drug for ischemic heart disease. The effects of sufentanil on MAPK signaling in ischemic heart disease were explored. The effects of sufentanil on ischemia-reperfusion (IR)-induced myocardial injury in a rat model were examined. The serum levels of ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-017-0127-y
更新日期:2018-02-01 00:00:00
abstract::Temperature reduction in CHO cell batch culture may be beneficial in the production of recombinant protein and in maintenance of viability. The effects on cell cycle, apoptosis and nucleotide pools were studied in cultures initiated at 37°C and temperature shifted to 30 °C after 48 hours. In control cultures maintaine...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1007919921991
更新日期:1997-01-01 00:00:00
abstract::To characterize the effects of cetyltriethylammonium bromide (CTAB) on the transcription of gp64 promoter from Bombyx mori nucleopolyhedrovirus (BmNPV), the plasmid pBmgp64Luc used in transient expression assay system was constructed by using the luciferase gene as a reporter under the control of BmNPV gp64 promoter. ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1024231023015
更新日期:2003-01-01 00:00:00
abstract::Hepatocytes self-assemble in culture to form compacted spherical aggregates, or spheroids, that mimic the structure of the liver by forming tight junctions and bile canalicular channels. Hepatocyte spheroids thus resemble the liver to a great extent. However, liver tissue contains other cell types and has bile ducts a...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-004-7996-6
更新日期:2004-07-01 00:00:00
abstract::MiR-548 has been reported to be involved in a variety of tumor processes, but its function in breast cancer remains unclear. In this study, we found that miR-548 was low expressed in breast cancer tissues and cells compared with normal control. We then examined whether up-regulation of miR-548 could improve the progre...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-020-00418-3
更新日期:2020-10-01 00:00:00
abstract::Endothelial cells are involved in important pathological situations. They could be the target for infectious processes as for example in Cowdriosis, an important disease in cattle due to the rickettsia Cowdria ruminantium prevalent in the south of the Sahara. They are also connected to angiogenic processes related to ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1007928203997
更新日期:1997-11-01 00:00:00
abstract::Protein delivery represents a powerful tool for experiments in live cells including studies of protein-protein interactions, protein interference with blocking antibodies, intracellular trafficking and protein or peptide biological functions. Most available reagents dedicated to the protein delivery allow efficient cr...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-007-9102-3
更新日期:2008-01-01 00:00:00
abstract::Dental pulp stem cells (DPSCs) are a type of mesenchymal stem cells, which have the self-renewal and multi-lineage differentiation potential, including chondrocytes, adipocytes, neural cells and osteoblasts. So they play a significant role in pulp repair and bone regeneration. Oncostatin M (OSM), one of the IL-6 famil...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-016-9995-9
更新日期:2016-12-01 00:00:00
abstract::The present study describes an immunocytochemistry (ICC) assay with self-raised hyperimmune sera and a Baby Hamster Kidney-21 (BHK-21) cell line infected with Porcine Sapelovirus (PSV). Sapelovivus/IVRI/SPF-c-6/2015 strain Indian PSV was isolated from the porcine IBRS-2 cell line and investigated for growth on non-por...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-019-00315-4
更新日期:2019-06-01 00:00:00
abstract::The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rb1 was produced by fusing splenocytes immunized with a ginsenoside Rb1- bovine serum albumin conjugate wit...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008068718392
更新日期:1999-03-01 00:00:00
abstract::A promising method for diabetes treatment is the implantation of immunoisolated cells secreting insulin in response to glucose. Cell availability limits the application of this approach at a medically-relevant scale. We explore the use of transformed cells that can be grown to large homogeneous populations in developi...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00762410
更新日期:1994-01-01 00:00:00
abstract::We developed a simple method for freezing anchorage-dependent cells, including primary cultured rat liver parenchymal cells, without detaching the cells from the culture dish. The method consists of preculture of the cells to confluence, changing the growth medium to a conventional freezing medium, packaging in a cont...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00556297
更新日期:1991-03-01 00:00:00
abstract::Dental pulp stem cells constitute an attractive source of multipotent mesenchymal stem cells owing to their high proliferation rate and multilineage differentiation potential. Osteogenesis is initiated by osteoblasts, which originate from mesenchymal stem cells. These cells express specific surface antigens that disap...
journal_title:Cytotechnology
pub_type: 杂志文章,评审
doi:10.1007/s10616-018-0225-5
更新日期:2018-10-01 00:00:00
abstract::Successful culturing of neurons from adult animals has been historically difficult for a relatively long time. In this study, we reported the development of a novel method for the isolation and the culture of major pelvic ganglion (MPG) neurons from adult rat. The cultured cells were identified by neuron morphology an...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-012-9515-5
更新日期:2013-08-01 00:00:00
abstract::We screened for biological activity which induces neurite outgrowth in vitro from 300 species of marine algae from along the Japan coast for possible use as a treatment for the lack of neurotrophic factor which is considered to be a cause of Alzheimer's disease. In this study, we evaluated the neurite outgrowth promot...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1023974323807
更新日期:2002-11-01 00:00:00
abstract::Early developments in serum-free media led to a variety of formulations in which components normally provided in serum and required for growth (insulin, transferrin, lipid supplements, trace elements) and poorly defined components (extracts, hydrolysates) were added to defined basal media. These additives were mostly ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-006-9002-y
更新日期:2006-03-01 00:00:00
abstract::Precision-cut liver tissue slices (PCLS) have been used for decades to study pharmacological metabolism as well as toxicology and efficacy of novel substances on primary material under standardized conditions. Slicing of primary liver tissue has been done using different slicing machines. Since there has been great va...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-009-9246-4
更新日期:2009-12-01 00:00:00