Abstract：
:The light and truncated heavy chains of human factor VIII, expressed separately in baculovirus-infected insect cells, exhibited different secretory behaviour when compared with each other and with a biologically active fusion molecule of the truncated heavy and light chains. The light chain was very efficiently secreted into culture medium, as judged by high extracellular protein levels and the absence of evidence for light chain retention within cells. Alternatively, proteins containing the heavy chain sequence were poorly secreted and appeared to be sequestered within cells, suggesting that regions within the heavy chain are responsible for the low levels of secreted protein which have generally been observed for recombinant factor VIII.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Webb E,Tkalcevic J,Edwards S,Hocking D,Nisbet Idoi
10.1007/BF02215666subject
Has Abstractpub_date
1996-01-01 00:00:00pages
165-70issue
2eissn
0920-9069issn
1573-0778journal_volume
21pub_type
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