Growth, metabolism and baculovirus production in suspension cultures of an Anticarsia gemmatalis cell line.


:The UFL-AG-286 cell line, established from embryonic tissue of the lepidopteran insect Anticarsia gemmatalis, has been identified as a good candidate to be used as a cellular substrate in the development of a process for in vitro production of the Anticarsia gemmatalis multicapsid nucleopolyhedrovirus, a baculovirus widely used as bioinsecticide. In order to characterize the technological properties of this cell line and evaluate its feasibility to use it for the large-scale production of Anticarsia gemmatalis multicapsid nucleopolyhedrovirus, UFL-AG-286 cells were adapted to grow as agitated suspension cultures in spinner-flasks. Batch suspension cultures of adapted cells in serum-supplemented TC-100 medium grew with a doubling time of about 29 h and reached a maximum cell density higher than 3.5 x 10(6) viable cells ml(-1). At the end of the growth period glucose was completely depleted from the culture medium, but L: -lactate was not produced. Amino acids, with the exception of glutamine, were only negligibly consumed or produced. In contrast to other insect cell lines, UFL-AG-286 cells appeared to be unable to synthesize alanine as a metabolic way to dispose the by-product ammonia. The synchronous infection of suspension cultures with Anticarsia gemmatalis multicapsid nucleopolyhedrovirus in the early to medium exponential growth phase yielded high amounts of both viral progenies per cell and reduced the specific demands of UFL-AG-286 cells for the main nutrients.






Gioria VV,Jäger V,Claus JD




Has Abstract


2006-10-01 00:00:00












  • Isolation, culture and differentiation of duck (Anas platyrhynchos) preadipocytes.

    abstract::In the present study, we isolated preadipocytes from the adipose tissue of Peking duck and subsequently cultured them in vitro. Cell counting kit-8 assay was employed to establish the growth curve of duck primary preadipocytes. Meanwhile, after the cells reaching full confluency, they were induced to differentiate int...


    pub_type: 杂志文章


    authors: Ding F,Li QQ,Li L,Gan C,Yuan X,Gou H,He H,Han CC,Wang JW

    更新日期:2015-10-01 00:00:00

  • Galangin induces B16F10 melanoma cell apoptosis via mitochondrial pathway and sustained activation of p38 MAPK.

    abstract::Galangin, an active flavonoid present at high concentration in Alpinia officinarum Hance and propolis, shows cytotoxicity towards several cancer cell lines, including melanoma. However, the specific cellular targets of galangin-induced cytotoxicity in melanoma are still unknown. Here, we investigated the effects of ga...


    pub_type: 杂志文章


    authors: Zhang W,Lan Y,Huang Q,Hua Z

    更新日期:2013-05-01 00:00:00

  • A modification of tooth germ cultivation in vitro and in ovo.

    abstract::Mandibular molar anlages excised from 17-day mouse foetuses were cultured in vitro or in ovo (on the chorioallantoic membrane). In both cases, the explants were underlain either with a Millipore filter or with a piece of fibrin foam. Tooth germs were harvested after 7 days of cultivation and processed histologically. ...


    pub_type: 杂志文章


    authors: Peterka M,Mandys V,Peterková R

    更新日期:1991-09-01 00:00:00

  • Chromatid segregation analysis in native human lymphocyte anaphases using sequential fluorescence in situ hybridization.

    abstract::A sequential multiprobe fluorescence in situ hybridization technique was developed to study the 13, 18, 21, X and Y chromatid segregation in human lymphocytes anaphases cultures without antimitotic treatment. This method was used to know if exist any different chromosomes segregation in lymphocytes from Down syndrome ...


    pub_type: 杂志文章


    authors: Escudero T,Coll MD,Fuster C,Egozcue J

    更新日期:2002-09-01 00:00:00

  • Explants of porcine coronary artery in culture: A paradigm for studying the influence of heparin on vascular wall cell proliferation.

    abstract::Explant cultures of porcine coronary artery provided a coculture model, used as a paradigm of arterial wall in contact with vascular prosthesis which allowed the study of spatial and temporal changes in cell phenotype. First cells emerging from the explant had an endothelial phenotype monitored by cytoimmunostaining. ...


    pub_type: 杂志文章


    authors: Dufresne M,Warocquier-Clérout R

    更新日期:2001-09-01 00:00:00

  • Vascular growth factor binding kinetics to the endothelial cell basement membrane, with a kinetics-based correction for substrate binding.

    abstract::Vascular growth factors, including vascular endothelial growth factor and fibroblast growth factor-2, bind to heparan sulfate proteoglycans in the basement membrane. While this binding, storage, and release system provides a critical model for controlled drug release devices, basement membrane-growth factor binding ki...


    pub_type: 杂志文章


    authors: Clyne AM,Edelman ER

    更新日期:2009-07-01 00:00:00

  • Versatile stem cells, young and old. A review.

    abstract::Both embryonic and somatic stem cells have been studied in recent years with particular regard to their differentiation potential. In vitro studies allow a considerable amplification of such cells in culture as well as the induction of commitment in different directions under proper stimulating factors. Moreover, a su...


    pub_type: 杂志文章


    authors: Eridani S

    更新日期:2001-03-01 00:00:00

  • Xanthoria elegans (Link) (lichen) extract counteracts DNA damage and oxidative stress of mitomycin C in human lymphocytes.

    abstract::Several lichen species have been used for medicinal purposes throughout the ages, and they are reported to be effective in the treatment of different disorders including ulcer and cancer. It is revealed that lichens may be easily accessible sources of natural drugs and possible food supplements after their safety eval...


    pub_type: 杂志文章


    authors: Turkez H,Aydin E,Aslan A

    更新日期:2012-12-01 00:00:00

  • Visible light regulates neurite outgrowth of nerve cells.

    abstract::The neurite outgrowth of PC12 cells on collagen-coated glass plates under light emitting diode (LED) irradiation at several wavelengths (i.e., 455, 470, 525, 600, 630, 880 and 945 nm) was investigated. No neurite outgrowth was observed during cultivation under irradiation from the lamp of an inverted light microscope ...


    pub_type: 杂志文章


    authors: Higuchi A,Watanabe T,Noguchi Y,Chang Y,Chen WY,Matsuoka Y

    更新日期:2007-07-01 00:00:00

  • Anticancer effect of Tamarix gallica extracts on human colon cancer cells involves Erk1/2 and p38 action on G2/M cell cycle arrest.

    abstract::Taking into account that oxidative stress is among the factors causing cancer-related death; chemoprevention which consists in using antioxidant substances such as phenolics could prevent cancer formation and progression. In the present study, phenolic contents and antioxidant activities of methanolic extracts from th...


    pub_type: 杂志文章


    authors: Boulaaba M,Tsolmon S,Ksouri R,Han J,Kawada K,Smaoui A,Abdelly C,Isoda H

    更新日期:2013-12-01 00:00:00

  • DNA and cell death.

    abstract::The type of DNA damage and the role of poly (ADP-ribosyl) polymerase (ADPRP) and sulphated glyprotein 2 (SGP-2) in programmed cell death (apoptosis) was investigated in the following model systems: i) rat thymocytes treated with dexamethasone (DEX) eitherin vitro orin vivo; ii) human perypheral blood mononuclear cells...


    pub_type: 杂志文章


    authors: Monti D,Troiano L,Tropea F,Grassilli E,Cossarizza A,Barozzi D,Bettuzzi S,Corti A,Franceschi C

    更新日期:1991-02-01 00:00:00

  • Development of a generic transient transfection process at 100 L scale.

    abstract::We have developed a generic transient transfection process at 100 L scale, using HEK293-EBNA cells and PEI as the transfection reagent for the production of recombinant IgG. The process, including large-scale plasmid preparation, expression at bioreactor scale, capture, purification and, if necessary, endotoxin remova...


    pub_type: 杂志文章


    authors: Tuvesson O,Uhe C,Rozkov A,Lüllau E

    更新日期:2008-02-01 00:00:00

  • Optimization of cultivation conditions for monoclonal IgM antibody production by M1A2 hybridoma using artificial neural network.

    abstract::Monoclonal antibody (McAb) has been established as one of the most successful therapeutic strategies for the treatment of cancer. M1A2 (McAb) as a new monoclonal antibody was designed to recognize heat shock protein (HSP60), but its optimum production condition has not been studied. In this study, the cell culture con...


    pub_type: 杂志文章


    authors: Bashokouh F,Abbasiliasi S,Tan JS

    更新日期:2019-07-16 00:00:00

  • A high-yielding serum-free, suspension cell culture process to manufacture recombinant adenoviral vectors for gene therapy.

    abstract::We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum free media and suspension growth. Subsequent...


    pub_type: 杂志文章


    authors: Schoofs G,Monica TJ,Ayala J,Horwitz J,Montgomery T,Roth G,Castillo FJ

    更新日期:1998-11-01 00:00:00

  • A luciferase complementation assay system using transferable mouse artificial chromosomes to monitor protein-protein interactions mediated by G protein-coupled receptors.

    abstract::G protein-coupled receptors (GPCRs) are seven-transmembrane domain receptors that interact with the β-arrestin family, particularly β-arrestin 1 (ARRB1). GPCRs interact with 33% of small molecule drugs. Ligand screening is promising for drug discovery concerning GPCR-related diseases. Luciferase complementation assay ...


    pub_type: 杂志文章


    authors: Uno N,Fujimoto T,Komoto S,Kurosawa G,Sawa M,Suzuki T,Kazuki Y,Oshimura M

    更新日期:2018-12-01 00:00:00

  • Highly passage of Spodoptera litura cell line causes its permissiveness to baculovirus infection.

    abstract::It is well known that the characteristics of cell lines possibly alter when cell lines are at high-passage number because of the environmental selection. We do not know whether non-permissive or low-permissive cell lines could become permissive or more permissive to virus infection after over-high passage. In the pres...


    pub_type: 杂志文章


    authors: Zhang X,Lan W,Deng Y,Ma Y,Liu K,Peng J,Li Y,Hong H

    更新日期:2008-07-01 00:00:00

  • Purification and characterization of alpha-D-galactosidase produced by ADG cell line established from abalon digestive gland.

    abstract::ADG cell line was established from an abalonedigestive gland and previously characterized. ADGcells have the potential to grow in protein-freeculture and secrete l3 types of glycosidases. Inthis article, we determined the origin of ADG cell line,using electron microscopy, and purified a glycosidasesecreted by these ce...


    pub_type: 杂志文章


    authors: Kusumoto K,Shirahata S,Kamei Y

    更新日期:2000-07-01 00:00:00

  • Genotoxicity of Thermopsis turcica on Allium cepa L. roots revealed by alkaline comet and random amplified polymorphic DNA assays.

    abstract::This study was undertaken to evaluate genotoxic potential of Thermopsis turcica aqueous extracts on the roots of onion bulb (Allium cepa L.) by comet assay and random amplified polymorphic DNA technique. The Allium root growth inhibition test indicated that the EC50 and 2×EC50 values were 8 and 16 mg/ml concentrations...


    pub_type: 杂志文章


    authors: Ciğerci İH,Cenkci S,Kargıoğlu M,Konuk M

    更新日期:2016-08-01 00:00:00

  • Improvement in the antibody productivity of human-human hybridomas by transfection with Tac gene.

    abstract::The presence of a highly purified recombinant interleukin-2 (rIL-2) increased the production of immunoglobulin (IgM or IgG) by human-human hybridomas to 1.5-2.0 times the production by untreated cells. However, these cells did not react with anti-Tac (IL-2 receptor alpha) antibody. To enhance the response of the hybri...


    pub_type: 杂志文章


    authors: Iwamoto K,Shintani Y,Sasada R,Honjo T,Kitano K

    更新日期:1990-07-01 00:00:00

  • The role of recombinant proteins in the development of serum-free media.

    abstract::Early developments in serum-free media led to a variety of formulations in which components normally provided in serum and required for growth (insulin, transferrin, lipid supplements, trace elements) and poorly defined components (extracts, hydrolysates) were added to defined basal media. These additives were mostly ...


    pub_type: 杂志文章


    authors: Keenan J,Pearson D,Clynes M

    更新日期:2006-03-01 00:00:00

  • Effects of Thymus kotschyanus var. glabrescens Boiss. extract on mitomycin-C induced chromosomal aberrations and sister chromatid exchanges in human lymphocytes.

    abstract::In this investigation, clastogenic effects of Thymus kotschyanus var. glabrescens Boiss. extract (TE) and anticlastogenic effects of this extract against Mitomycin C (MMC) induced chromosome damage have been evaluated in human peripheral blood lymphocytes in vitro. Two series of experiments were conducted. In the firs...


    pub_type: 杂志文章


    authors: Celik M,Unal F,Yüzbaşıoğlu D,Yılmaz S,Aksoy H,Karaman S

    更新日期:2006-06-01 00:00:00

  • The role of oncostatin M regulates osteoblastic differentiation of dental pulp stem cells through STAT3 pathway.

    abstract::Dental pulp stem cells (DPSCs) are a type of mesenchymal stem cells, which have the self-renewal and multi-lineage differentiation potential, including chondrocytes, adipocytes, neural cells and osteoblasts. So they play a significant role in pulp repair and bone regeneration. Oncostatin M (OSM), one of the IL-6 famil...


    pub_type: 杂志文章


    authors: Feng X,Shen S,Cao P,Zhu L,Zhang Y,Zheng K,Feng G,Zhang D

    更新日期:2016-12-01 00:00:00

  • Primary cell culture from embryos of the Japanese scallop Mizuchopecten yessoensis (Bivalvia).

    abstract::Primary cell cultures obtained from embryos of Mizuchopecten yessoensis (Bivalvia) survived for four months. Although the number of cells progressively decreased during the cultivation, mitotic cells were observed both at the first stages and at the end. A possibility of growing marine invertebrates cells in long term...


    pub_type: 杂志文章


    authors: Odintsova NA,Khomenko AV

    更新日期:1991-05-01 00:00:00

  • Epithelial properties of human intestinal Caco-2 cells cultured in a serum-free medium.

    abstract::Human intestinal Caco-2 cells were cultured under serum-free conditions on an insoluble collagen and FCS matrix (Caco-2-SF), and a comparison was made between several characteristics of Caco-2 and Caco-2-SF cells. Their morphological appearance was identical. Slight differences were found in cell growth and expression...


    pub_type: 杂志文章


    authors: Hashimoto K,Shimizu M

    更新日期:1993-01-01 00:00:00

  • Evaluating the bottlenecks of recombinant IgM production in mammalian cells.

    abstract::Despite the fact, that monoclonal antibodies are the fastest growing group of biopharmaceuticals in development, this is not true for the IgM class, which remains as enigmatic as ever. While more examples of usefulness of IgMs for medical applications are emerging, their recombinant production is still not common. In ...


    pub_type: 杂志文章


    authors: Chromikova V,Mader A,Steinfellner W,Kunert R

    更新日期:2015-03-01 00:00:00

  • Uptake and efflux of FL118 and two FL118 derivatives in 3D cell model.

    abstract::Drug uptake and efflux are two of the critical factors required in order to be able to define drug efficacy. This study aims to investigate cytotoxicity and uptake mechanisms of two FL118 analogues (7-Q20 and val-FL118) in parallel with FL118 in three-dimensional multi-cellular spheroids model. The influence of compou...


    pub_type: 杂志文章


    authors: Zhou L,Weng Q,Zheng Y,Zhou Y,Li Q,Li F

    更新日期:2019-07-12 00:00:00

  • Apoptosis-inducing activities of Halopteris scoparia L. Sauvageau (Brown algae) on cancer cells and its biosafety and antioxidant properties.

    abstract::The aim of this study was to reveal the biological activities and in vivo toxicity profiles of n-hexane, chloroform and methanol extracts of brown algae Halopteris scoparia L. Sauvageau. In this study, extracts were tested for their phytochemical contents and antioxidant activities. The cytotoxic activities of the ext...


    pub_type: 杂志文章


    authors: Güner A,Nalbantsoy A,Sukatar A,Karabay Yavaşoğlu NÜ

    更新日期:2019-06-01 00:00:00

  • Interaction ofEscherichia coli cytotoxic necrotizing factor type 1 (CNF1) with cultured cells.

    abstract::The cytotoxic necrotizing factor type 1 (CNF1) fromE. coli causes necrosis in rabbit skin and multinucleation in cultured cells. Cells exposed to CNF1 were characterized by changes in actin organization, mainly consisting in the presence of well-developed stress fibers and membrane ruffles. The interaction of CNF1 wit...


    pub_type: 杂志文章


    authors: Falzano L,Fiorentini C,Boquet P,Donelli G

    更新日期:1993-01-01 00:00:00

  • Different expression levels of TNF receptors on the rheumatoid synovial macrophages derived from surgery and a synovectomy as detected by a new flow cytometric analysis.

    abstract::TNFalpha plays a crucial role in the pathogenesis of rheumatoid arthritis. It is very important to examine the expression of the TNF receptors, the ligand of TNFalpha. In this study, we developed a triple-color flow cytometric analysis using CD45 and CD14 monoclonal antibodies to simply detect the expression of the TN...


    pub_type: 杂志文章


    authors: Ida H,Aramaki T,Nakamura H,Fujikawa K,Arima K,Tamai M,Kamachi M,Satoh K,Origuchi T,Kawakami A,Furuichi I,Kawabe Y,Eguchi K

    更新日期:2009-07-01 00:00:00

  • Dental pulp stem cells and osteogenesis: an update.

    abstract::Dental pulp stem cells constitute an attractive source of multipotent mesenchymal stem cells owing to their high proliferation rate and multilineage differentiation potential. Osteogenesis is initiated by osteoblasts, which originate from mesenchymal stem cells. These cells express specific surface antigens that disap...


    pub_type: 杂志文章,评审


    authors: Mortada I,Mortada R

    更新日期:2018-10-01 00:00:00