Abstract:
:Vascular growth factors, including vascular endothelial growth factor and fibroblast growth factor-2, bind to heparan sulfate proteoglycans in the basement membrane. While this binding, storage, and release system provides a critical model for controlled drug release devices, basement membrane-growth factor binding kinetics have not been fully established. We modified endothelial cell-growth factor binding kinetics protocols for the basement membrane. The basement membrane showed low affinity for fibroblast growth factor-2 (K d = 185.8 nM), with a slow off rate (k off = 0.00338 min(-1)). However, results were confounded by growth factor binding to tissue culture polystyrene in a manner strikingly similar to basement membrane. Since substrate binding could not be blocked, a binding kinetics based correction technique was developed to account for polystyrene growth factor binding. This method was validated by conducting binding kinetics experiments on bacteriologic plates that exhibit little growth factor binding. This novel method will improve our understanding of cell and protein interaction with the basement membrane in health and disease. They can also further be applied to develop biomimetic drug delivery systems.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Clyne AM,Edelman ERdoi
10.1007/s10616-009-9212-1subject
Has Abstractpub_date
2009-07-01 00:00:00pages
33issue
1-3eissn
0920-9069issn
1573-0778pii
10.1007/s10616-009-9212-1journal_volume
60pub_type
杂志文章相关文献
CYTOTECHNOLOGY文献大全abstract::A silicon microsensor (ISFET - Ion Sensitive Field Effect Transistor) has been used to detect the metabolism of a cell population cultured on a coverslip and positioned close to the sensor surface. The system output is analyzed as a function of cell density. ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00746064
更新日期:1993-01-01 00:00:00
abstract::Arrowroot (Maranta arundinacea. L) is an underutilized local crop potentially to be developed as carbohydrate source and functional food in Indonesia. The objectives of this research are to evaluate the immunostimulatory effects of arrowroot extracts in vitro by using animal cell culture techniques, and in vivo by usi...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-011-9403-4
更新日期:2012-03-01 00:00:00
abstract::Structure-activity relationship of antioxidants for the protective effects on linoleic acid hydroperoxide (LOOH)-induced toxicity were examined in cultured human umbilical vein endothelial cells. alpha-Tocopherol, 2,2,5,7,8-pentamethylchroman-6-ol, butylated hydroxytoluene, probucol, and fatty acid esters of ascorbic ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008139412588
更新日期:2001-01-01 00:00:00
abstract::Protein delivery represents a powerful tool for experiments in live cells including studies of protein-protein interactions, protein interference with blocking antibodies, intracellular trafficking and protein or peptide biological functions. Most available reagents dedicated to the protein delivery allow efficient cr...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-007-9102-3
更新日期:2008-01-01 00:00:00
abstract::We have examined the effect of differentiation on the expression of different members of the transforming growth factor type-beta (TGF-beta) family using embryonal carcinoma (EC) cells and early mammalian embryos. We determined that TGF-beta activity increases approximately 25-100% when the mouse EC cell line, F9, is ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00563783
更新日期:1990-11-01 00:00:00
abstract::Understanding the growth dynamics of influenza viruses is an essential step in virus replication and cell-adaptation. The aim of this study was to elucidate the growth kinetic of a low pathogenic avian influenza H9N2 subtype in chicken embryo fibroblast (CEF) and chicken tracheal epithelial (CTE) cells during consecut...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-012-9495-5
更新日期:2013-05-01 00:00:00
abstract::This work focused on determining the effect of dissolved oxygen concentration (DO) on growth and metabolism of BHK-21 cell line (host cell for recombinant proteins manufacturing and viral vaccines) cultured in two stirred tank bioreactors with different aeration-homogenization systems, as well as pH control mode. BHK-...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-013-9612-0
更新日期:2014-08-01 00:00:00
abstract::The bone marrow represents the most common source from which to isolate mesenchymal stem cells (MSCs). MSCs are capable of differentiating into tissues of the three primary lineages and have the potential to enhance repair in damaged organs through the principals of regenerative medicine. Given the ease with which MSC...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-012-9443-4
更新日期:2012-10-01 00:00:00
abstract::A fructose-based cell culture is suitable for the process control of protein production because of slow sugar consumption rate and low lactate accumulation. The fructose transporter, GLUT5, mediates its incorporation into cells and is required for the fructose-based culture. In order to produce efficiently recombinant...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-010-9289-6
更新日期:2010-08-01 00:00:00
abstract::In recent years, water pollution has been converted to a challenging discussion in health area of human being. Heavy elements are one of the most important water pollutants and their negative adverse effects on body systems have been confirmed. In this study, investigation of effects of two heavy elements including le...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-013-9626-7
更新日期:2014-10-01 00:00:00
abstract::It is well known that the characteristics of cell lines possibly alter when cell lines are at high-passage number because of the environmental selection. We do not know whether non-permissive or low-permissive cell lines could become permissive or more permissive to virus infection after over-high passage. In the pres...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-008-9158-8
更新日期:2008-07-01 00:00:00
abstract::The CHO cell line has achieved considerable commercial importance as a vehicle for the production of human therapeutic proteins, but is known to lack a functional copy of the gene coding for α2,6-sialyltransferase (EC 2.4.99.1). The cDNA for rat α2,6-ST was expressed in a recombinant CHO cell line making interferon-γ,...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00353939
更新日期:1996-01-01 00:00:00
abstract::This article describes the historical development of the prophylactic use of antibiotics in cell culture as well as their effects on cells. The influence of antibiotics on cell morphology, cellular degeneration and cell death and cellular function is summarized. Cellular DNA as well as protein synthesis are affected w...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00749764
更新日期:1995-06-01 00:00:00
abstract::The chicken is a useful animal for the development of the specificantibodies against the mammalian conserved proteins. We generated twotypes of recombinant chicken monoclonal antibodies (mAbs), using a phagedisplay technique from a chicken hybridoma HUC2-13 which secreted themAb to the N-terminal of the mammalian prio...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008149815908
更新日期:2000-03-01 00:00:00
abstract::The effects of media concentrations of glucose andglutamine on the intracellular nucleotide pools andoxygen uptake rates of a murine antibody-secretinghybridoma cell line were investigated. Cells takenfrom mid-exponential phase of growth were incubated inmedium containing varying concentrations of glucose(0-25 mM) and...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008154615643
更新日期:2000-10-01 00:00:00
abstract::The aim of this research was a comparative study on the isolation and culture of head kidney macrophages derived from Acipenser persicous and Rutilus frisii kutum as teleost and chondrostei species of fish. The macrophages were isolated by density gradient sedimentation, followed by adherence to a plastic surface. The...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-018-0256-y
更新日期:2018-12-01 00:00:00
abstract::By employing the affinity gel fraction technique, we have detected a 175 kDa tyrosine-O-sulfate (TyrS)-binding protein in sodium choleate extracts of the microsomal membrane fractions of bovine liver and pancreas, as well as canine liver and pancreas. Western blot analysis revealed the presence of the bovine liver Tyr...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1007903315687
更新日期:1997-01-01 00:00:00
abstract::A Xenopus laevis peptidyl C-terminal alpha-amidating enzyme (AE-II) gene, modified by deletion of a region encoding the putative membrane-spanning domain and the putative C-terminal cytosolic tail, was expressed in BoMo-15 AIIc insect cells and silkworm larvae using a Bombyx mori baculovirus expression vector system. ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF02525492
更新日期:1992-01-01 00:00:00
abstract::The establishment of a cell culture system promoting chondrocyte differentiation has been utilized to better characterize phenotypic stages of chondrogenesis at the cellular level. Although the expression of the type II collagen gene has been studied during "in vitro" chondrocyte differentiation, little is known about...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00736804
更新日期:1991-02-01 00:00:00
abstract::We describe a novel fully automated high-throughput time-lapse microscopy system and evaluate its performance for precisely tracking the motility of several glioma and osteoblastic cell lines. Use of this system revealed cell motility behavior not discernable with conventional techniques by collecting data (1) from cl...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-006-9006-7
更新日期:2006-05-01 00:00:00
abstract::Perilla frutescens is an Asian dietary herb consumed as an essential seasoning in Japanese cuisine as well as used for a Chinese medicine. Here, we report that a newly found methoxyflavanone derivative from P. frutescens (Perilla-derived methoxyflavanone, PDMF; 8-hydroxy-5,7-dimethoxyflavanone) shows carcinostatic act...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-017-0116-1
更新日期:2018-06-01 00:00:00
abstract::In neuronal dendrites, septins localize to the base of the spine, a unique position which is sandwiched between the microtubule (MT)-rich dendritic shaft and the actin filament-rich spine. Here, we provide evidence for the association of SEPT6 with MTs in cultured rat hippocampal neurons. In normal cultures, SEPT6 clu...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-012-9477-7
更新日期:2013-03-01 00:00:00
abstract::In construction of epithelial cells as multilayers, the cells are grown submerged to confluence on fibroblast-embedded collagen gels and, then, lifted to air to promote their stratification. We recently demonstrated that gingival epithelial cells form uniform monolayers on semi-permeable nitrocellulose membranes, supp...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-016-0029-4
更新日期:2016-12-01 00:00:00
abstract::Photopolymerizable hydrogels offer great potential in cartilage tissue engineering due to their ability to conform to irregular defect shapes and be applied in a potentially minimally invasive manner. An important process requirement in the use of photopolymerizable hydrogels is the ability of the suspended cells to w...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-012-9512-8
更新日期:2013-08-01 00:00:00
abstract::The decline of European abalone Haliotis tuberculata populations has been associated with various pathogens including bacteria of the genus Vibrio. Following the summer mortality outbreaks reported in France between 1998 and 2000, Vibrio harveyi strains were isolated from moribund abalones, allowing in vivo and in vit...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-013-9583-1
更新日期:2013-10-01 00:00:00
abstract::Electroporation is a way to induce nanometersized membrane pore for exogenous substances delivery into cytoplasm using an artificial electric field. Now it was widely used for molecules transfer especially in molecular experiments and genetic aspects. In recent years, modern electroporation on the embryo was developed...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-007-9082-3
更新日期:2007-06-01 00:00:00
abstract::Toxicity testing is required for new chemicals being introduced onto the market. The use of animals in evaluating chemical safety is costly and time consuming. Furthermore, there is the ethical need to develop alternative methods to reduce the required number of animals. The new in vitro assays offer numerous advantag...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::The cytotoxic necrotizing factor type 1 (CNF1) fromE. coli causes necrosis in rabbit skin and multinucleation in cultured cells. Cells exposed to CNF1 were characterized by changes in actin organization, mainly consisting in the presence of well-developed stress fibers and membrane ruffles. The interaction of CNF1 wit...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00746055
更新日期:1993-01-01 00:00:00
abstract::Specific respiration rate ( [Formula: see text]) is a key parameter to understand cell metabolism and physiological state, providing useful information for process supervision and control. In this work, we cultivated different insect cells in a very controlled environment, being able to measure [Formula: see text]. Sp...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-007-9118-8
更新日期:2008-05-01 00:00:00
abstract::Primary rat hepatocytes dedifferentiate rapidly losing theactivities of the drug metabolising enzymes involved in thedetoxification of xenobiotics in the liver. An alternativeapproach to using primary hepatocytes for toxicity testing isthe development of immortalised hepatocyte cell lines via thetransfection of primar...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008159912634
更新日期:2000-10-01 00:00:00