Abstract:
:A human continuous cell line (huGK-14) within a lineage of passaged cultures was investigated in the mode of integration and expression of hepatitis B virus (HBV) genes. HBV DNA was integrated in eight different sites of the cellular DNA, in each of which HBV genome was rearranged, fragmented, and/or partly deleted. Complete HBV genome that may lead to production of infectious virus particles was not detected in the cells nor in the culture medium. Clones of cDNA containing a complete coding frame for small HBs antigen protein (type adr) were obtained from mRNA of the cells. The cells were stable over the period of six months of cultivation and more than 60 population doublings in the mode of HBV integration and HBs mRNA expression.These results provide substantial evidence for the absence of an ability for the integrated DNA to create an infectious product in the cell; for the stable production of HBs mRNA from the cells, and suggest the usefulness of this cell line as a substrate for HBV vaccine production.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Nakamichi N,Noda A,Yonezu T,Koike K,Matsumura Tdoi
10.1023/A:1007924119018subject
Has Abstractpub_date
1997-11-01 00:00:00pages
61-70issue
1-3eissn
0920-9069issn
1573-0778journal_volume
25pub_type
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