Structure and expression of integrated hepatitis B virus genes in an HBs antigen producing human cell line (huGK-14).

Abstract:

:A human continuous cell line (huGK-14) within a lineage of passaged cultures was investigated in the mode of integration and expression of hepatitis B virus (HBV) genes. HBV DNA was integrated in eight different sites of the cellular DNA, in each of which HBV genome was rearranged, fragmented, and/or partly deleted. Complete HBV genome that may lead to production of infectious virus particles was not detected in the cells nor in the culture medium. Clones of cDNA containing a complete coding frame for small HBs antigen protein (type adr) were obtained from mRNA of the cells. The cells were stable over the period of six months of cultivation and more than 60 population doublings in the mode of HBV integration and HBs mRNA expression.These results provide substantial evidence for the absence of an ability for the integrated DNA to create an infectious product in the cell; for the stable production of HBs mRNA from the cells, and suggest the usefulness of this cell line as a substrate for HBV vaccine production.

journal_name

Cytotechnology

journal_title

Cytotechnology

authors

Nakamichi N,Noda A,Yonezu T,Koike K,Matsumura T

doi

10.1023/A:1007924119018

subject

Has Abstract

pub_date

1997-11-01 00:00:00

pages

61-70

issue

1-3

eissn

0920-9069

issn

1573-0778

journal_volume

25

pub_type

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