Detection of mycoplasma contaminations by the polymerase chain reaction.

abstract：:The ratio of hapten to bovine serum albumin (BSA) in an antigen conjugate was determined by matrix-assisted laser desorption/ionization (MALDI) tof mass spectrometry. A hybridoma secreting monoclonal antibody (MAb) was produced by fusing splenocytes immunized with an antigen-BSA conjugate with HAT-sensitive mouse myel...

journal_title：Cytotechnology

authors： Shoyama Y,Tanaka H,Fukuda N

更新日期：1999-09-01 00:00:00

abstract：:There is an unmet medical need for the improvement of pancreatic islet maintenance in culture. Due to restricted donor availability it is essential to ameliorate islet viability and graft engraftment. The aim of this study was to compare the standard tissue culture techniques with the advanced Real Architecture For 3D...

journal_title：Cytotechnology

authors： Szebeni GJ,Tancos Z,Feher LZ,Alfoldi R,Kobolak J,Dinnyes A,Puskas LG

更新日期：2017-04-01 00:00:00

abstract：:Hepatocytes self-assemble in culture to form compacted spherical aggregates, or spheroids, that mimic the structure of the liver by forming tight junctions and bile canalicular channels. Hepatocyte spheroids thus resemble the liver to a great extent. However, liver tissue contains other cell types and has bile ducts a...

journal_title：Cytotechnology

authors： Abu-Absi SF,Hansen LK,Hu WS

更新日期：2004-07-01 00:00:00

abstract：:Gingival fibroblast cultures from four patients with Down's Syndrome (DS) and periodontal disease were compared with four in vitro age-matched fibroblast cultures of handicapped subjects (ND) also affected by periodontitis. The extra copy of chromosome 21 could alter growth regulation and biochemical mechanisms, so we...

journal_title：Cytotechnology

authors： Solmi R,Rossetti A,Talassi O,Tomasini GL,Fato R,Estornell E,Lucarini G,Lenaz G,Simonelli L,Brunelli MA

更新日期：1993-01-01 00:00:00

abstract：:In the present study, we isolated preadipocytes from the adipose tissue of Peking duck and subsequently cultured them in vitro. Cell counting kit-8 assay was employed to establish the growth curve of duck primary preadipocytes. Meanwhile, after the cells reaching full confluency, they were induced to differentiate int...

journal_title：Cytotechnology

authors： Ding F,Li QQ,Li L,Gan C,Yuan X,Gou H,He H,Han CC,Wang JW

更新日期：2015-10-01 00:00:00

abstract：:Human umbilical cord-derived mesenchymal stem cells (hUMSCs) hold strong self-renewal capacity and low immunogenicity, which have attracted attention as potential candidates for bone repair and regeneration. However, insufficient osteogenic differentiation markedly hinders the clinical applications of hUMSCs. In the p...

journal_title：Cytotechnology

authors： Liu J,Wang H,Ren W,Zhou Y,Ye Z,Tan WS

更新日期：2020-10-01 00:00:00

abstract：:The efficacy of hepatocellular carcinoma (HCC) treatment is very low because of the high percentage of recurrence and resistance to anticancer agents. Hepatic cancer stem cells (HCSCs) are considered the origin of such recurrence and resistance. Our aim was to evaluate the stemness of doxorubicin and 5-fluorouracil re...

journal_title：Cytotechnology

authors： Vu NB,Nguyen TT,Tran LC,Do CD,Nguyen BH,Phan NK,Pham PV

更新日期：2013-08-01 00:00:00

abstract：:Hypertension (HT) is a common and life threating health problem worldwide leading to stroke, heart attack and renal failure. It is characterized by elevated blood pressure forced heart load. Human interleukin-6 (IL-6) and C- reactive protein (CRP) are known to be involved in inflammatory processes. IL-6 gene is a poly...

journal_title：Cytotechnology

authors： Karaman E,Urhan Kucuk M,Bayramoglu A,Uzun Göçmen S,Ercan S,Guler HI,Kucukkaya Y,Erden S

更新日期：2015-12-01 00:00:00

abstract：:Vasculogenesis, the formation of blood vessels in embryonic or fetal tissue mediated by immature vascular cells (i.e., angioblasts), is poorly understood. Here we report a summary of our recent studies on the identification of a population of vascular progenitor cells (VPCs) in human fetal aorta. These undifferentiate...

journal_title：Cytotechnology

authors： Invernici G,Madeddu P,Emanueli C,Parati EA,Alessandri G

更新日期：2008-09-01 00:00:00

abstract：:A new compound, rotenoid isoflavone glycoside named, 6'-O-beta-D: -glucopyranosyl-12a-hydroxydalpanol was isolated from the methanolic (MeOH) fruit extract of Amorpha fruticosa LINNE by means of multi-stage column chromatography. Immuno-modulatory activities of this new glycoside were compared with the partitioned fra...

journal_title：Cytotechnology

authors： Lee HJ,Kang HY,Kim CH,Kim HS,Kwon MC,Kim SM,Shin IS,Lee HY

更新日期：2006-11-01 00:00:00

abstract：:The propagation of foot-and-mouth disease virus on BHK-21 suspension cells, although economically convenient, may yield a scarcely immunizing antigen. Helpful insights were obtained by investigating a few genotypic and phenotypic features of the cell cultures. The appearance of polyploid populations, higher cell conce...

journal_title：Cytotechnology

authors： Amadori M,Berneri C

更新日期：1993-01-01 00:00:00

abstract：:We describe a novel fully automated high-throughput time-lapse microscopy system and evaluate its performance for precisely tracking the motility of several glioma and osteoblastic cell lines. Use of this system revealed cell motility behavior not discernable with conventional techniques by collecting data (1) from cl...

journal_title：Cytotechnology

authors： Fotos JS,Patel VP,Karin NJ,Temburni MK,Koh JT,Galileo DS

更新日期：2006-05-01 00:00:00

abstract：:Chemical genomics is a new research paradigm with importantapplications in drug discovery. It links genomic targets withsmall-molecule chemistries thereby allowing for efficient targetvalidation and lead compound identification. ACADIA'schemical-genomics platform consists of a large and diverse small-moleculelibrary (...

journal_title：Cytotechnology

authors： Hacksell U,Nash N,Burstein ES,Piu F,Croston G,Brann MR

更新日期：2002-01-01 00:00:00

abstract：:This paper reviews the JRC-Ispra research activity on toxicological studies related to the environmental and occupational exposure of trace metals, as carried out by a combination of cell culture methodologies with nuclear and radioanalytical techniques. Applications concern the setting of uptake-effect relationships ...

journal_title：Cytotechnology

authors： Sabbioni E,Pozzi G,Fischbach M,Bahbouth E

更新日期：1993-01-01 00:00:00

abstract：:The aim of this study was to reveal the biological activities and in vivo toxicity profiles of n-hexane, chloroform and methanol extracts of brown algae Halopteris scoparia L. Sauvageau. In this study, extracts were tested for their phytochemical contents and antioxidant activities. The cytotoxic activities of the ext...

journal_title：Cytotechnology

authors： Güner A,Nalbantsoy A,Sukatar A,Karabay Yavaşoğlu NÜ

更新日期：2019-06-01 00:00:00

abstract：:We have previously demonstrated the radio-sensitizing effect of zoledronic acid (ZOL), a third generation bisphosphonate, on human esophageal squamous cell carcinoma (ESCC) cells. Here we show that ZOL suppresses metastatic progression of ESCC cells mainly through up-regulating the tight junction protein occludin. Exp...

journal_title：Cytotechnology

authors： Lin C,Xin S,Qin X,Li H,Lin L,You Y

更新日期：2016-08-01 00:00:00

abstract：:Benfuracarb is a carbamate insecticide used to control insect pests in vegetables and it has anti-acetylcholinesterase activity lower than other carbamates. Cytotoxic effects of benfuracarb were evaluated by using root growth inhibition (EC50), mitotic index (MI), and mitotic phase determinations on the root meristem ...

journal_title：Cytotechnology

authors： Eren Y,Erdoğmuş SF,Akyıl D,Özkara A

更新日期：2016-08-01 00:00:00

abstract：:A simple protein free medium was formulated and tested in suspension culture using three hybridoma cell lines. The medium, referred to as CDSS (Chemically Defined Serum Substitutes), consisted of the basal medium DMEM:Ham F12, 1:1, with HEPES (D12H), plus pluronic F68, trace elements, ferric citrate, ascorbic acid, an...

journal_title：Cytotechnology

authors： Qi YM,Greenfield PF,Reid S

更新日期：1996-06-01 00:00:00

abstract：:Protein delivery represents a powerful tool for experiments in live cells including studies of protein-protein interactions, protein interference with blocking antibodies, intracellular trafficking and protein or peptide biological functions. Most available reagents dedicated to the protein delivery allow efficient cr...

journal_title：Cytotechnology

authors： Weill CO,Biri S,Adib A,Erbacher P

更新日期：2008-01-01 00:00:00

abstract：:It is well known that the characteristics of cell lines possibly alter when cell lines are at high-passage number because of the environmental selection. We do not know whether non-permissive or low-permissive cell lines could become permissive or more permissive to virus infection after over-high passage. In the pres...

journal_title：Cytotechnology

authors： Zhang X,Lan W,Deng Y,Ma Y,Liu K,Peng J,Li Y,Hong H

更新日期：2008-07-01 00:00:00

abstract：:The low-metastatic RSV-transformed fibrosarcoma line B77-3T3 and its metastatic variant AA12, selected in vitro, have been analysed by blot and in situ hybridization with v-src and murine c-myc specific probes in order to detect molecular rearrangements underlying the transition from the low-metastatic to the high-met...

journal_title：Cytotechnology

authors： Doneda L,Custode P,Morghen Cde G,Larizza L

更新日期：1987-10-01 00:00:00

abstract：:Many cytological studies have reported that the numbers of binucleated cells were elevated in various tumors. However, binucleated cells are observed in not only malignant tumors but also normal tissues. Thus, the clinical significance of binucleated cells is controversial. Here we attempted to elucidate the character...

journal_title：Cytotechnology

authors： Nishimura K,Watanabe S,Hayashida R,Sugishima S,Iwasaka T,Kaku T

更新日期：2016-08-01 00:00:00

abstract：:This study demonstrated a newly developed method using adipose tissue-derived stromal cells (ADSCs) and hydroxypropylmethylcellulose (HPMC) in building injectable tissue engineered cartilage in vivo. ADSCs from rabbit subcutaneous fatty tissue were cultured in chondrogenic differentiation medium and supplemented with ...

journal_title：Cytotechnology

authors： Xu Y,Zhang J,Ma Y,Han Y,Min J,Liang Y,Zhao D,Qiu J

更新日期：2014-10-01 00:00:00

abstract：:Several lichen species have been used for medicinal purposes throughout the ages, and they are reported to be effective in the treatment of different disorders including ulcer and cancer. It is revealed that lichens may be easily accessible sources of natural drugs and possible food supplements after their safety eval...

journal_title：Cytotechnology

authors： Turkez H,Aydin E,Aslan A

更新日期：2012-12-01 00:00:00

abstract：:Human-human hybridoma SH-76 cells were found to produce a factor that supported the growth of lymphocytic cells at low densities. The factor was purified from serum-free conditioned medium of the hybridoma cells by a successive application of ammonium sulfate precipitation, DEAE-Toyopearl, TSK G3000 SW and DEAE-5PW co...

journal_title：Cytotechnology

authors： Miyata M,Murakami H,Hashizume S,Yamada K,Omura H

更新日期：1988-11-01 00:00:00

abstract：:The benzofuran lignans egonol and homoegonol are found in all species of the genus Styrax. Since natural products are important sources of new anticancer drugs, this study evaluated the cytotoxic activity of a hydroalcoholic extract of the stems of S. camporum (SCHE) and their chemical markers, egonol (EG) and homoego...

journal_title：Cytotechnology

authors： de Oliveira PF,Damasceno JL,Bertanha CS,Araújo AR,Pauletti PM,Tavares DC

更新日期：2016-08-01 00:00:00

abstract：:To explore the role of ribosomal protein S15A (RPS15A) in breast cancer. The Oncomine database was used to compare the expression of RPS15A in human breast cancer tissues and normal tissues. RPS15A in breast cancer cell line ZR-75-30 and BT474 was specifically knocked down using lentivirus-mediated short hairpin RNAs ...

journal_title：Cytotechnology

authors： Feng W,Liang C,Wang C,Yu X,Li Q,Yang H

更新日期：2018-10-01 00:00:00

abstract：:Material and degree of reductance balance equations are used to estimate the rates of oxygen uptake and carbon dioxide evolution of animal cell cultures. Lumped compositions, molecular weight and reductance degree of cellular protein, monoclonal antibody, biomass and amino acid consumption (excluding glutamine and ala...

journal_title：Cytotechnology

authors： Xiu ZL,Deckwer WD,Zeng AP

更新日期：1999-05-01 00:00:00

abstract：:Expression of mRNAs encoding ten laminin chain variants was studied by reverse transcription-polymerase chain reaction (RT-PCR) of mRNA extracted from cultured mouse blastocysts. alpha1, beta1 and gamma1 mRNAs, of which products form laminin-1, were the only chains expressed in blastocysts before hatching. alpha4 and ...

journal_title：Cytotechnology

authors： Azimi M,Niimi T,Yoshida N,Kitagawa Y

更新日期：1999-09-01 00:00:00

abstract：:The usefulness of host range expanded viruses as an expressionvector system was investigated by following the expression ofthe E. coli lacZ gene. The host range expanded recombinantviruses were obtained from Sf-21 or BmN-4 cells coinfected withAutographa californica and Bombyx mori nuclearpolyhedrosis viruses. Among t...

journal_title：Cytotechnology

authors： Kim HS,Woo SD,Kim WJ,Choi JY,Kang SK

更新日期：2000-02-01 00:00:00