Abstract：
:Protein delivery represents a powerful tool for experiments in live cells including studies of protein-protein interactions, protein interference with blocking antibodies, intracellular trafficking and protein or peptide biological functions. Most available reagents dedicated to the protein delivery allow efficient crossing of the plasma membrane. Nevertheless, the major disadvantage for these reagents is a weak release of the delivered protein into the cytoplasm. In this publication we demonstrate efficient protein delivery with a non-peptide based reagent, in human epithelial carcinoma HeLa cells and primary human skin fibroblasts. Using a fluorescent protein in combination with fluorescence microscopy and fluorescence-assisted cell sorting analysis, we show that the delivered protein is indeed released effectively in the cytoplasm, as expected for a dedicated carrier. Furthermore, we present a step-by-step method to optimize conditions for successful intracellular protein delivery.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Weill CO,Biri S,Adib A,Erbacher Pdoi
10.1007/s10616-007-9102-3subject
Has Abstractpub_date
2008-01-01 00:00:00pages
41-8issue
1eissn
0920-9069issn
1573-0778journal_volume
56pub_type
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