Human fetal aorta-derived vascular progenitor cells: identification and potential application in ischemic diseases.

abstract：CONCLUSIONS:With the wide availability of insect cell culture media, it can generally be considered a routine process to develop new cell lines. Exceptions to this statement do exist, of course. Difficulties may arise when attempting to culture a specific cell type. For example, while there are a few cell lines from in...

journal_title：Cytotechnology

authors： Lynn DE

更新日期：1996-01-01 00:00:00

abstract：:The growth of rat adrenal nerve cells was remarkably enhanced by supplementing the cultured medium from the human fibroblast cell line, Hs 68. Maximum specific growth rate and length of the neurites were observed as 0.076 (1/hr) and 0.026 mm, respectively in 20% supplement of five day old medium. In adding more than 2...

journal_title：Cytotechnology

authors： Hong JS,Kim DS,Kim SH,Choi DH,Lee JH,Lee HY

更新日期：1998-03-01 00:00:00

abstract：:Light scattering properties of hybridoma cells were examined with flow cytometry. Viable and dead cells form two distinct populations. The distribution of the two populations changes during a batch culture. the concentration of dead cells measured by flow cytometry correlates well to that measured by hemacytometer. Th...

journal_title：Cytotechnology

authors： Sen S,Srienc F,Hu WS

更新日期：1989-05-01 00:00:00

abstract：:There may exist a connection between Echinococcus granulosus infection and cancer development. Here, it is aimed to investigate specific effects of E. granulosus protoscoleces (PSCs) on the proliferation and invasion capacities of hepatocellular carcinoma (HCC) cells in vitro and ex vitro. HepG2 cells were cultured wi...

journal_title：Cytotechnology

authors： Yasen A,Wang M,Ran B,Lv G,Aji T,Xiao H,Shao Y,Wen H

更新日期：2021-02-01 00:00:00

abstract：:Monoclonal antibodies (mAb) are high added value glycoproteins recommended for immunotherapy, diagnosis, and also for the treatment of bacterial infections resistant to multiple drugs such as Methicillin Resistant Staphylococcus aureus (MRSA). In addition to environmental conditions related to cell cultures, the intri...

journal_title：Cytotechnology

authors： Corrêa AL,Senna JP,de Sousa ÁP

更新日期：2016-05-01 00:00:00

abstract：:Animal cells in suspension culture can suffer severe mechanical damage from bursting gas bubbles or other hydrodynamic force sources. Certain chemical additives in the culture media, particularly some surface-active chemicals, can effectively protect animal cells against such damage. Previously we proposed that the pr...

journal_title：Cytotechnology

authors： Wu J

更新日期：1996-01-01 00:00:00

abstract：:The neurite outgrowth of PC12 cells on collagen-coated glass plates under light emitting diode (LED) irradiation at several wavelengths (i.e., 455, 470, 525, 600, 630, 880 and 945 nm) was investigated. No neurite outgrowth was observed during cultivation under irradiation from the lamp of an inverted light microscope ...

journal_title：Cytotechnology

authors： Higuchi A,Watanabe T,Noguchi Y,Chang Y,Chen WY,Matsuoka Y

更新日期：2007-07-01 00:00:00

abstract：:The capacity of hepatocytes and cholangiocytes to contribute to their own maintenance has long been recognized. More recently, studies have indicated the presence of both intra-hepatic and extra-hepatic stem/progenitor cell populations. The intraorgan compartment probably derives primarily from the biliary tree, most ...

journal_title：Cytotechnology

authors： Theise ND

更新日期：2003-03-01 00:00:00

abstract：:We describe possible functions of carbohydrates attached to growth factors and strategies to examine the functions, concentrating on erythropoietin, a major regulator of erythropoiesis. Erythropoietin in erythropoiesis functions as an endocrine hormone; it is produced by kidney cells and transferred into the circulati...

journal_title：Cytotechnology

authors： Nagao M,Masuda S,Ueda M,Sasaki R

更新日期：1995-01-01 00:00:00

abstract：:To explore the role of ribosomal protein S15A (RPS15A) in breast cancer. The Oncomine database was used to compare the expression of RPS15A in human breast cancer tissues and normal tissues. RPS15A in breast cancer cell line ZR-75-30 and BT474 was specifically knocked down using lentivirus-mediated short hairpin RNAs ...

journal_title：Cytotechnology

authors： Feng W,Liang C,Wang C,Yu X,Li Q,Yang H

更新日期：2018-10-01 00:00:00

abstract：:Previous studies have demonstrated antidiabetic effects for rooibos (Aspalathus linearis) and aspalathin (ASP), one of its main polyphenols. Rooibos, an endemic plant of South Africa, is well-known for its use as herbal tea. Green ('unfermented') rooibos has been shown to contain more ASP than 'fermented' rooibos tea,...

journal_title：Cytotechnology

authors： Kamakura R,Son MJ,de Beer D,Joubert E,Miura Y,Yagasaki K

更新日期：2015-08-01 00:00:00

abstract：:This paper reviews the JRC-Ispra research activity on toxicological studies related to the environmental and occupational exposure of trace metals, as carried out by a combination of cell culture methodologies with nuclear and radioanalytical techniques. Applications concern the setting of uptake-effect relationships ...

journal_title：Cytotechnology

authors： Sabbioni E,Pozzi G,Fischbach M,Bahbouth E

更新日期：1993-01-01 00:00:00

abstract：:The type of DNA damage and the role of poly (ADP-ribosyl) polymerase (ADPRP) and sulphated glyprotein 2 (SGP-2) in programmed cell death (apoptosis) was investigated in the following model systems: i) rat thymocytes treated with dexamethasone (DEX) eitherin vitro orin vivo; ii) human perypheral blood mononuclear cells...

journal_title：Cytotechnology

authors： Monti D,Troiano L,Tropea F,Grassilli E,Cossarizza A,Barozzi D,Bettuzzi S,Corti A,Franceschi C

更新日期：1991-02-01 00:00:00

abstract：:G protein-coupled receptors (GPCRs) are seven-transmembrane domain receptors that interact with the β-arrestin family, particularly β-arrestin 1 (ARRB1). GPCRs interact with 33% of small molecule drugs. Ligand screening is promising for drug discovery concerning GPCR-related diseases. Luciferase complementation assay ...

journal_title：Cytotechnology

authors： Uno N,Fujimoto T,Komoto S,Kurosawa G,Sawa M,Suzuki T,Kazuki Y,Oshimura M

更新日期：2018-12-01 00:00:00

abstract：:Daphnoretin is a bicoumarin compound isolated from a natural product, Wikstroemia indica, which has been used to treat many diseases. It has strong antiviral and anti-tumor activities. Taking the anti-tumor activity of daphnoretin as a starting point, the present study aimed to test the pro-apoptotic effect of daphnor...

journal_title：Cytotechnology

authors： Yang ZY,Kan JT,Cheng ZY,Wang XL,Zhu YZ,Guo W

更新日期：2014-01-01 00:00:00

abstract：:A novel type of succinoyl trehalose lipid (STL-1) prepared from n-hexadecane-culture of Rhodococcus erythropolis SD-74 markedly inhibited the growth of a human monocytoid leukemic cell line, U937, and induced its morphological alteration along a monocyte-macrophage lineage. STL-1 markedly increased differention-associ...

journal_title：Cytotechnology

authors： Isoda H,Shinmoto H,Matsumura M,Nakahara T

更新日期：1995-01-01 00:00:00

abstract：:Structure-activity relationship of antioxidants for the protective effects on linoleic acid hydroperoxide (LOOH)-induced toxicity were examined in cultured human umbilical vein endothelial cells. alpha-Tocopherol, 2,2,5,7,8-pentamethylchroman-6-ol, butylated hydroxytoluene, probucol, and fatty acid esters of ascorbic ...

journal_title：Cytotechnology

authors： Kaneko T,Baba N,Matsuo M

更新日期：2001-01-01 00:00:00

abstract：:Primary cell cultures obtained from embryos of Mizuchopecten yessoensis (Bivalvia) survived for four months. Although the number of cells progressively decreased during the cultivation, mitotic cells were observed both at the first stages and at the end. A possibility of growing marine invertebrates cells in long term...

journal_title：Cytotechnology

authors： Odintsova NA,Khomenko AV

更新日期：1991-05-01 00:00:00

abstract：:Alphaviruses are positive stranded RNA viruses that replicate to extremely high titers. Sindbis and Semliki Forest viral vectors are widely used tools for high-level production of recombinant proteins. Recent studies have broadened their scope to vaccine production, gene therapy, and analysis of cell function. Here we...

journal_title：Cytotechnology

authors： Boorsma M,Koller D,Renner WA,Bachmann MF

更新日期：2001-05-01 00:00:00

abstract：:The subcellular localisation of doxorubicin and Victoria Blue BO (VBBO) in a murine mammary tumour cell line EMT6-S, and the resistant sub-lineEMT6-R was studied, using confocal microscopy, in order to investigate their sites of action. In cells treated with doxorubicin (10 mu M) for 90 min, the pattern of intracellul...

journal_title：Cytotechnology

authors： Burrow SM,Phoenix DA,Wainwright M,Tobin MJ

更新日期：2002-05-01 00:00:00

abstract：:Fibroblast growth factors (FGFs) have been implicated in a number of proliferative lesions, including malignant tumor growth and vascularization. As a result, cytotoxic agents that target cell surface FGF receptors are currently under investigation. Previous reports have shown that conjugation of basic FGF with the ri...

journal_title：Cytotechnology

authors： Miller K,Wilder PJ,Rizzino A

更新日期：1993-01-01 00:00:00

abstract：:Dental pulp stem cells constitute an attractive source of multipotent mesenchymal stem cells owing to their high proliferation rate and multilineage differentiation potential. Osteogenesis is initiated by osteoblasts, which originate from mesenchymal stem cells. These cells express specific surface antigens that disap...

journal_title：Cytotechnology

authors： Mortada I,Mortada R

更新日期：2018-10-01 00:00:00

abstract：:The ratio of hapten to bovine serum albumin (BSA) in an antigen conjugate was determined by matrix-assisted laser desorption/ionization (MALDI) tof mass spectrometry. A hybridoma secreting monoclonal antibody (MAb) was produced by fusing splenocytes immunized with an antigen-BSA conjugate with HAT-sensitive mouse myel...

journal_title：Cytotechnology

authors： Shoyama Y,Tanaka H,Fukuda N

更新日期：1999-09-01 00:00:00

abstract：:Electroporation is a way to induce nanometersized membrane pore for exogenous substances delivery into cytoplasm using an artificial electric field. Now it was widely used for molecules transfer especially in molecular experiments and genetic aspects. In recent years, modern electroporation on the embryo was developed...

journal_title：Cytotechnology

authors： Yuan TF

更新日期：2007-06-01 00:00:00

abstract：:A new bioreactor for animal cell cultivation employs two compartments for cells and medium respectively. The two chambers are separated by an ultrafiltration membrane. Cells and solution of collagen or collagen/chitosan mixture were loaded to the cell chamber and were allowed to form gel inside. Contraction of the cel...

journal_title：Cytotechnology

authors： Scholz M,Hu WS

更新日期：1990-09-01 00:00:00

abstract：:Vascular growth factors, including vascular endothelial growth factor and fibroblast growth factor-2, bind to heparan sulfate proteoglycans in the basement membrane. While this binding, storage, and release system provides a critical model for controlled drug release devices, basement membrane-growth factor binding ki...

journal_title：Cytotechnology

authors： Clyne AM,Edelman ER

更新日期：2009-07-01 00:00:00

abstract：:An efficient mammalian cell system for producing bioproducts should retain high cell viability and efficient use of energy sources rendering the need to understand the effects of various variables on the cell system. In this study, global metabolite (metabolomics) analysis approach was used to try and understand the r...

journal_title：Cytotechnology

authors： Mohmad-Saberi SE,Hashim YZ,Mel M,Amid A,Ahmad-Raus R,Packeer-Mohamed V

更新日期：2013-08-01 00:00:00

abstract：:Menadione-catalyzed H(2)O(2) production by viable animal cells was proportional to the viable cell number, and H(2)O(2) production decreased with increasing cytotoxic effects after the incubation of cells with cytotoxic compounds. The cytotoxic effects of food additives, pesticides, antibiotics, heavy metals, phytotox...

journal_title：Cytotechnology

authors： Yamashoji S,Isshiki K

更新日期：2001-11-01 00:00:00

abstract：:The cytotoxic necrotizing factor type 1 (CNF1) fromE. coli causes necrosis in rabbit skin and multinucleation in cultured cells. Cells exposed to CNF1 were characterized by changes in actin organization, mainly consisting in the presence of well-developed stress fibers and membrane ruffles. The interaction of CNF1 wit...

journal_title：Cytotechnology

authors： Falzano L,Fiorentini C,Boquet P,Donelli G

更新日期：1993-01-01 00:00:00

abstract：:The statistics of estimators used with the endpoint assay for virus titration were investigated. For a standard assay with 10 wells/dilution, the graphical estimator traditionally used was found to produce estimates with significant positive bias and a relatively low accuracy. Furthermore, the graphical estimator was ...

journal_title：Cytotechnology

authors： Nielsen LK,Smyth GK,Greenfield PF

更新日期：1992-01-01 00:00:00