Abstract:
:Advancements in cell cultures are occurring at a rapid pace, an important direction is culturing cells in 3D conditions. We demonstrate the usefulness of agarose hydrogels in obtaining 3 dimensional aggregates of three cell lines, A549, MCF-7 and Sp2/0. The differences in culture phases, susceptibility to cisplatin-induced cytotoxicity are studied. Also, the 3D aggregates of the three cell lines were reverted into 2D cultures and the protein profile differences among the 2D, 3D and revert cultures were studied. The analysis of protein profile differences using UniProt data base further augment the usefulness of agarose hydrogels for obtaining 3D cell cultures.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Ravi M,Kaviya SR,Paramesh Vdoi
10.1007/s10616-014-9795-zsubject
Has Abstractpub_date
2016-05-01 00:00:00pages
429-41issue
3eissn
0920-9069issn
1573-0778pii
10.1007/s10616-014-9795-zjournal_volume
68pub_type
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