Impact of chicken-origin cells on adaptation of a low pathogenic influenza virus.

Abstract:

:Understanding the growth dynamics of influenza viruses is an essential step in virus replication and cell-adaptation. The aim of this study was to elucidate the growth kinetic of a low pathogenic avian influenza H9N2 subtype in chicken embryo fibroblast (CEF) and chicken tracheal epithelial (CTE) cells during consecutive passages. An egg-adapted H9N2 virus was seeded into both cell culture systems. The amount of infectious virus released into the cell culture supernatants at interval times post-infection were titered and plaque assayed. The results as well as cell viability results indicate that the infectivity of the influenza virus was different among these primary cells. The egg-adapted H9N2 virus featured higher infectivity in CTE than in CEF cells. After serial passages and plaque purifications of the virus, a CTE cell-adapted strain was generated which carried amino acid substitutions within the HA stem region. The strain showed faster replication kinetics in cell culture resulting in an increase in virus titer. Overall, the present study provides the impact of cell type, multiplicity of infection, cellular protease roles in virus infectivity and finally molecular characterization during H9N2 virus adaptation procedure.

journal_name

Cytotechnology

journal_title

Cytotechnology

authors

Shahsavandi S,Ebrahimi MM,Mohammadi A,Zarrin Lebas N

doi

10.1007/s10616-012-9495-5

subject

Has Abstract

pub_date

2013-05-01 00:00:00

pages

419-24

issue

3

eissn

0920-9069

issn

1573-0778

journal_volume

65

pub_type

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