Abstract:
:The establishment of a cell culture system promoting chondrocyte differentiation has been utilized to better characterize phenotypic stages of chondrogenesis at the cellular level. Although the expression of the type II collagen gene has been studied during "in vitro" chondrocyte differentiation, little is known about the expression of the gene coding for its receptor: anchorin CII. The modulation of the anchorin mRNA steady state level in chick embryo chondrocytes at different developmental stages is described here.The anchorin mRNA level was low in dedifferentiated chondrocytes, progressively increased after the cell transfer into suspension (a condition promoting differentiation), reached its maximal value after 4 weeks and decreased after 5 weeks.Therefore anchorin CII mRNA reaches its maximum level in hypertrophic stage II chondrocytes.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Castagnola P,Cancedda Rdoi
10.1007/BF00736804subject
Has Abstractpub_date
1991-02-01 00:00:00pages
41-4issue
Suppl 1eissn
0920-9069issn
1573-0778journal_volume
5pub_type
杂志文章相关文献
CYTOTECHNOLOGY文献大全abstract::The advancement of immunocytochemistry (ICC) allows one to observe detailed spatial distribution of cellular antigens, but, with some limitations. Using conventional ICC, it is difficult to distinguish the nuclear localization from cytoplasm, as two large subcellular compartments overlap on the z-axis. In this study, ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-010-9266-0
更新日期:2010-04-01 00:00:00
abstract::To determine the function of miR-206 in epilepsy. Epileptic rat model was established by intra-amygdala injection of kainic acid (KA). Expression levels of miR-206, C-C Motif Chemokine Ligand 2 (CCL2) and interleukin-1β (Il-1β) in hippocampus tissues was measured by reverse transcription-quantitative PCR (RT-qPCR) and...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-019-00324-3
更新日期:2019-06-26 00:00:00
abstract::Both embryonic and somatic stem cells have been studied in recent years with particular regard to their differentiation potential. In vitro studies allow a considerable amplification of such cells in culture as well as the induction of commitment in different directions under proper stimulating factors. Moreover, a su...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1017526408890
更新日期:2001-03-01 00:00:00
abstract::A hallmark of human cancer cells is immortal cell growth, which is associated with telomere maintenance by telomerase. The transcriptional regulation of the human telomerase reverse transcriptase (hTERT) gene is a major mechanism that negatively and positively controls telomerase activity in normal and cancer cells, r...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-004-5122-4
更新日期:2004-06-01 00:00:00
abstract::A murine hybridoma line (Zac3), secreting an IgA monoclonal antibody, was cultivated in different systems: a BALB/c mouse, a T-flask, a stirred-tank bioreactor and a hollow fiber reactor. These systems were characterized in terms of cell metabolism and performances for IgA production. Cultures in T-flask and batch bio...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00749221
更新日期:1995-02-01 00:00:00
abstract::Obesity, mainly characterized by the excess fat storage, is a global health problem resulting in serious morbidity and mortality. Identification of molecular mechanisms in adipogenic differentiation pathway might lead to development of new strategies for diagnosis, prevention and therapy of obesity and associated dise...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-016-0047-2
更新日期:2017-02-01 00:00:00
abstract::This study was undertaken to evaluate genotoxic potential of Thermopsis turcica aqueous extracts on the roots of onion bulb (Allium cepa L.) by comet assay and random amplified polymorphic DNA technique. The Allium root growth inhibition test indicated that the EC50 and 2×EC50 values were 8 and 16 mg/ml concentrations...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-014-9835-8
更新日期:2016-08-01 00:00:00
abstract::Photopolymerizable hydrogels offer great potential in cartilage tissue engineering due to their ability to conform to irregular defect shapes and be applied in a potentially minimally invasive manner. An important process requirement in the use of photopolymerizable hydrogels is the ability of the suspended cells to w...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-012-9512-8
更新日期:2013-08-01 00:00:00
abstract::It is well known that the characteristics of cell lines possibly alter when cell lines are at high-passage number because of the environmental selection. We do not know whether non-permissive or low-permissive cell lines could become permissive or more permissive to virus infection after over-high passage. In the pres...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-008-9158-8
更新日期:2008-07-01 00:00:00
abstract::The development of specific catalytic inhibitors for the serine protease urokinase-type plasminogen activator (uPA) has been hindered due to difficulties in producing sufficient amounts of active recombinant uPA that is catalytically equivalent to native uPA. The purpose of this study was to develop an efficient syste...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-005-4637-7
更新日期:2005-09-01 00:00:00
abstract::Dental pulp stem cells constitute an attractive source of multipotent mesenchymal stem cells owing to their high proliferation rate and multilineage differentiation potential. Osteogenesis is initiated by osteoblasts, which originate from mesenchymal stem cells. These cells express specific surface antigens that disap...
journal_title:Cytotechnology
pub_type: 杂志文章,评审
doi:10.1007/s10616-018-0225-5
更新日期:2018-10-01 00:00:00
abstract::Sorbaria kirilowii (Regel) Maxim, a plant found in China, Korea, Japan, and east of Europe, is a common herb used for traditional medicinal purposes. However, its ability to prevent photoaging has not been studied. In this study, we investigated the anti-photoaging functions of an ethanol extract (Sk-EE) of S. kirilow...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-020-00449-w
更新日期:2021-02-01 00:00:00
abstract::Helichrysum Mill. (Asteraceae) species have been used in folk medicine for thousands of years in the world. The in vitro cytogenetic effects in human lymphocytes of nine Helichrysum taxa used in Turkey folk medicine were investigated. Blood samples were obtained from healthy donors, non-smoking volunteers, which were ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-009-9193-0
更新日期:2009-01-01 00:00:00
abstract::Cisplatin is one of the most effective chemotherapeutic agents but injury may occur at higher doses. The aim of this study was to investigate the effect of bilberry on cisplatin induced toxic effects in rat ovary. Twenty-one female Wistar-Albino rats were utilized to form three groups: In group 1 (control group), each...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-013-9621-z
更新日期:2014-08-01 00:00:00
abstract::Actions of chlorogenic acid, a major component of coffee, andits constituents, caffeic and quinic acids, on theproliferation and invasion of AH109A, a rat ascites hepatomacell line, were investigated using in vitro assay systems. Allthree components suppressed the AH109A invasion atconcentrations of 5-40 muM without a...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008141918852
更新日期:2000-07-01 00:00:00
abstract::In the last 10 years, new assignments and the special demands of mammalian cells to the culture conditions caused the development of complex small scale fermentation setups. The use of continuous fermentation and cell retention devices requires appropriate process control systems. An arrangement for control and data-a...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00762402
更新日期:1994-01-01 00:00:00
abstract::Tea catechin is one of the compounds that are closely related to obesity and insulin sensitivity. In order to determine the effect of catechin on adipocyte differentiation, we treated 3T3-L1 preadipocytes with different kinds of catechins. Our results showed that catechins, especially epigallocatechin gallate (EGCG), ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-010-9285-x
更新日期:2010-07-01 00:00:00
abstract::Expression of mRNAs encoding ten laminin chain variants was studied by reverse transcription-polymerase chain reaction (RT-PCR) of mRNA extracted from cultured mouse blastocysts. alpha1, beta1 and gamma1 mRNAs, of which products form laminin-1, were the only chains expressed in blastocysts before hatching. alpha4 and ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008084508489
更新日期:1999-09-01 00:00:00
abstract::Prostacyclin alternatively called prostaglandin (PG) I2 is an unstable metabolite synthesized by the arachidonate cyclooxygenase pathway. Earlier studies have suggested that prostacyclin analogues can act as a potent effector of adipose differentiation. However, biosynthesis of PGI2 has not been determined comprehensi...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-013-9616-9
更新日期:2014-08-01 00:00:00
abstract::In this investigation, clastogenic effects of Thymus kotschyanus var. glabrescens Boiss. extract (TE) and anticlastogenic effects of this extract against Mitomycin C (MMC) induced chromosome damage have been evaluated in human peripheral blood lymphocytes in vitro. Two series of experiments were conducted. In the firs...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-006-9015-6
更新日期:2006-06-01 00:00:00
abstract::We modified an existing protocol to develop a more efficient method to acquire and culture muscle-derived stem cells (MDSCs) and compared the characteristics of cells obtained from the two methods. This method is based on currently used multistep enzymatic digestion and preplate technique. During the replating process...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-018-0262-0
更新日期:2018-12-01 00:00:00
abstract::In this article, we describe a new method that facilitates to isolate mammalian cells inducible hyper-producing heterologous proteins. This method uses the tetracycline-inducible system to express both the selection marker and the heterologous gene, therefore, allows to increase the selection pressure by reducing the ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1023945517446
更新日期:2002-11-01 00:00:00
abstract::Vasculogenesis, the formation of blood vessels in embryonic or fetal tissue mediated by immature vascular cells (i.e., angioblasts), is poorly understood. Here we report a summary of our recent studies on the identification of a population of vascular progenitor cells (VPCs) in human fetal aorta. These undifferentiate...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-008-9167-7
更新日期:2008-09-01 00:00:00
abstract::A promising method for diabetes treatment is the implantation of immunoisolated cells secreting insulin in response to glucose. Cell availability limits the application of this approach at a medically-relevant scale. We explore the use of transformed cells that can be grown to large homogeneous populations in developi...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00762410
更新日期:1994-01-01 00:00:00
abstract::Fibroblast growth factor 21 (FGF21) potentially regulates glucose and lipid metabolism in energy homeostasis. We investigated dynamic changes in goat adipocytes treated with 75 nM FGF21 for 24, 36 and 48 h. Compared to controls, FGF21-treated adipocytes displayed smaller lipid droplets and altered levels of the mRNA t...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-018-0237-1
更新日期:2018-10-01 00:00:00
abstract::Transient gene expression (TGE) bioprocesses have been difficult to scale up in large stirred tank bioreactors with volumes of more than 1.5 L. Low production levels are often observed, but the causes have not been investigated (Gutierrez-Granados et al. in Crit Rev Biotechnol 38:918-940, 2018). Chikungunya Virus-like...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-019-00346-x
更新日期:2019-12-01 00:00:00
abstract::The subcellular localisation of doxorubicin and Victoria Blue BO (VBBO) in a murine mammary tumour cell line EMT6-S, and the resistant sub-lineEMT6-R was studied, using confocal microscopy, in order to investigate their sites of action. In cells treated with doxorubicin (10 mu M) for 90 min, the pattern of intracellul...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1022435829894
更新日期:2002-05-01 00:00:00
abstract::Numerous challenges remain in the successful clinical translation of cell-based therapeutic studies for skeletal tissue repair, including appropriate cell sources and viable cell delivery systems. Poly(ethylene glycol)-poly(ε-caprolactone) (PEG-PCL) amphiphilic block copolymers have been extensively explored in micros...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-012-9450-5
更新日期:2012-12-01 00:00:00
abstract::For the mouse hybridoma cell line VO 208, kinetics of growth, consumption of glucose and glutamine, and production of lactate, ammonia and antibodies were compared in batch and continuous cultures. At a given specific growth rate, different metabolic activities were observed: a 40% lower glucose and glutamine consumpt...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00570891
更新日期:1992-01-01 00:00:00
abstract::Carvacrol, the predominant monoterpene in many essential oils of Labitae including Origanum, Satureja, Thymbra, Thymus, and Corydothymus has substantial antibacterial, antifungal, antihelmintic, insecticial, analgesic and antioxidant activities. Approximately 75% of lung cancer is non-small cell carcinoma (NSCLC) whic...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/b:cyto.0000039917.60348.45
更新日期:2003-11-01 00:00:00