Abstract:
:For the mouse hybridoma cell line VO 208, kinetics of growth, consumption of glucose and glutamine, and production of lactate, ammonia and antibodies were compared in batch and continuous cultures. At a given specific growth rate, different metabolic activities were observed: a 40% lower glucose and glutamine consumption rate, but a 70% higher antibody production rate in continuous than in batch culture. Much higher metabolic rates were also measured during the initial lag phase of the batch culture. When representing the variation of the specific antibody production rate as a function of the specific growth rate, there was a positive association between growth and antibody production in the batch culture, but a negative association during the transient phase of the continuous culture. The kinetic differences between cellular metabolism in batch and continuous cultures may be the result of modifications in the physiology and metabolism of cells which, in continuous cultures, were extensively exposed to glucose limitations.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Goergen JL,Marc A,Engasser JMdoi
10.1007/BF00570891subject
Has Abstractpub_date
1992-01-01 00:00:00pages
147-55issue
2eissn
0920-9069issn
1573-0778journal_volume
10pub_type
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