Culture of major pelvic ganglion neurons from adult rat.

abstract：:Human skin has continuous self-renewal potential throughout adult life and serves as first line of defence. Its cellular components such as human epidermal keratinocytes (HEKs) and dermal mesenchymal stromal cells (DMSCs) are valuable resources for wound healing applications and cell based therapies. Here we show a si...

journal_title：Cytotechnology

authors： Mahabal S,Konala VB,Mamidi MK,Kanafi MM,Mishra S,Shankar K,Pal R,Bhonde R

更新日期：2016-08-01 00:00:00

abstract：:Monoclonal antibodies (mAb) are high added value glycoproteins recommended for immunotherapy, diagnosis, and also for the treatment of bacterial infections resistant to multiple drugs such as Methicillin Resistant Staphylococcus aureus (MRSA). In addition to environmental conditions related to cell cultures, the intri...

journal_title：Cytotechnology

authors： Corrêa AL,Senna JP,de Sousa ÁP

更新日期：2016-05-01 00:00:00

abstract：:This work focused on determining the effect of dissolved oxygen concentration (DO) on growth and metabolism of BHK-21 cell line (host cell for recombinant proteins manufacturing and viral vaccines) cultured in two stirred tank bioreactors with different aeration-homogenization systems, as well as pH control mode. BHK-...

journal_title：Cytotechnology

authors： Núñez EG,Leme J,de Almeida Parizotto L,Chagas WA,de Rezende AG,da Costa BL,Monteiro DC,Boldorini VL,Jorge SA,Astray RM,Pereira CA,Caricati CP,Tonso A

更新日期：2014-08-01 00:00:00

abstract：:Cell cycle, cell size and rhodamine 123 fluorescence in cell populations of two batch cultures were analysed and quantified with a fluorescence-activated cell sorter (FACS). Two cultures derived from either exponential or stationary phase innocula were investigated in order to demonstrate the dependency of the subsequ...

journal_title：Cytotechnology

authors： al-Rubeai M,Chalder S,Bird R,Emery AN

更新日期：1991-11-01 00:00:00

abstract：:We describe a novel fully automated high-throughput time-lapse microscopy system and evaluate its performance for precisely tracking the motility of several glioma and osteoblastic cell lines. Use of this system revealed cell motility behavior not discernable with conventional techniques by collecting data (1) from cl...

journal_title：Cytotechnology

authors： Fotos JS,Patel VP,Karin NJ,Temburni MK,Koh JT,Galileo DS

更新日期：2006-05-01 00:00:00

abstract：:Daphnoretin is a bicoumarin compound isolated from a natural product, Wikstroemia indica, which has been used to treat many diseases. It has strong antiviral and anti-tumor activities. Taking the anti-tumor activity of daphnoretin as a starting point, the present study aimed to test the pro-apoptotic effect of daphnor...

journal_title：Cytotechnology

authors： Yang ZY,Kan JT,Cheng ZY,Wang XL,Zhu YZ,Guo W

更新日期：2014-01-01 00:00:00

abstract：:The Pseudopimelodidae family comprises 35 species however, cytogenetic studies have been performed in only six species. This study uncovered karyotypic data on Pseudopimelodus pulcher and Microglanis cottoides. Both species possessed 2n = 54, with 20m + 16sm + 10st + 8a and FN = 100 for P. pulcher and 30m + 14sm + 6st...

journal_title：Cytotechnology

authors： Gouveia JG,de Moraes VP,Pires LB,da Rosa R,Dias AL

更新日期：2015-03-01 00:00:00

abstract：:Tea catechin is one of the compounds that are closely related to obesity and insulin sensitivity. In order to determine the effect of catechin on adipocyte differentiation, we treated 3T3-L1 preadipocytes with different kinds of catechins. Our results showed that catechins, especially epigallocatechin gallate (EGCG), ...

journal_title：Cytotechnology

authors： Kim H,Hiraishi A,Tsuchiya K,Sakamoto K

更新日期：2010-07-01 00:00:00

abstract：:A differentiation method of human bone marrow mesenchymal stem cells (MSCs) to chondrocytes was developed for the construction of a three-dimensional (3D) cartilage tissue. The adhesive cells, which were isolated from a human bone marrow aspirate were embedded in type I collagen in a poly-L: -lactate-glycolic acid cop...

journal_title：Cytotechnology

authors： Matsuda C,Takagi M,Hattori T,Wakitani S,Yoshida T

更新日期：2005-01-01 00:00:00

abstract：:Advancements in cell cultures are occurring at a rapid pace, an important direction is culturing cells in 3D conditions. We demonstrate the usefulness of agarose hydrogels in obtaining 3 dimensional aggregates of three cell lines, A549, MCF-7 and Sp2/0. The differences in culture phases, susceptibility to cisplatin-in...

journal_title：Cytotechnology

authors： Ravi M,Kaviya SR,Paramesh V

更新日期：2016-05-01 00:00:00

abstract：:The growth of rat adrenal nerve cells was remarkably enhanced by supplementing the cultured medium from the human fibroblast cell line, Hs 68. Maximum specific growth rate and length of the neurites were observed as 0.076 (1/hr) and 0.026 mm, respectively in 20% supplement of five day old medium. In adding more than 2...

journal_title：Cytotechnology

authors： Hong JS,Kim DS,Kim SH,Choi DH,Lee JH,Lee HY

更新日期：1998-03-01 00:00:00

abstract：:Fibroblast growth factors (FGFs) have been implicated in a number of proliferative lesions, including malignant tumor growth and vascularization. As a result, cytotoxic agents that target cell surface FGF receptors are currently under investigation. Previous reports have shown that conjugation of basic FGF with the ri...

journal_title：Cytotechnology

authors： Miller K,Wilder PJ,Rizzino A

更新日期：1993-01-01 00:00:00

abstract：:Previously, it has been shown that the binding of epidermal growth factor (EGF) by a wide range of cells decreases as cell density increases. In this report, we demonstrate that KB cells treated chronically with phorbol esters continue to exhibit decreases in EGF receptor binding as cell density increases. This findin...

journal_title：Cytotechnology

authors： Rizzino A,Huebert C,Kuszynski C,Wilder PJ

更新日期：1991-10-01 00:00:00

abstract：:Benfuracarb is a carbamate insecticide used to control insect pests in vegetables and it has anti-acetylcholinesterase activity lower than other carbamates. Cytotoxic effects of benfuracarb were evaluated by using root growth inhibition (EC50), mitotic index (MI), and mitotic phase determinations on the root meristem ...

journal_title：Cytotechnology

authors： Eren Y,Erdoğmuş SF,Akyıl D,Özkara A

更新日期：2016-08-01 00:00:00

abstract：:"In vitro" studies, commonly using porcine ovarian follicles, may generate inconsistent results when atretic follicles are not eliminated from the pool of experimental follicles. The present experiment was conducted to test the practical value of the macroscopie identification of large porcine follicles, which were as...

journal_title：Cytotechnology

authors： Dudek D

更新日期：1994-01-01 00:00:00

abstract：:We found that the formation of multilayer rosettes by transformed human blood lymphocytes after phytohemagglutinin (PHA) stimulation is correlated with conformational changes of the chromatin as seen by premature chromosome condensation (PCC). The frequency distribution of grades of PCC and multilayer rosette formatio...

journal_title：Cytotechnology

authors： Thomou H,Sambani C,Kitsiou P,Politis G

更新日期：1988-07-01 00:00:00

abstract：:Arrowroot (Maranta arundinacea. L) is an underutilized local crop potentially to be developed as carbohydrate source and functional food in Indonesia. The objectives of this research are to evaluate the immunostimulatory effects of arrowroot extracts in vitro by using animal cell culture techniques, and in vivo by usi...

journal_title：Cytotechnology

authors： Kumalasari ID,Harmayani E,Lestari LA,Raharjo S,Asmara W,Nishi K,Sugahara T

更新日期：2012-03-01 00:00:00

abstract：:A human continuous cell line (huGK-14) within a lineage of passaged cultures was investigated in the mode of integration and expression of hepatitis B virus (HBV) genes. HBV DNA was integrated in eight different sites of the cellular DNA, in each of which HBV genome was rearranged, fragmented, and/or partly deleted. C...

journal_title：Cytotechnology

authors： Nakamichi N,Noda A,Yonezu T,Koike K,Matsumura T

更新日期：1997-11-01 00:00:00

abstract：:We developed a simple method for freezing anchorage-dependent cells, including primary cultured rat liver parenchymal cells, without detaching the cells from the culture dish. The method consists of preculture of the cells to confluence, changing the growth medium to a conventional freezing medium, packaging in a cont...

journal_title：Cytotechnology

authors： Ohno T,Saijo-Kurita K,Miyamoto-Eimori N,Kurose T,Aoki Y,Yosimura S

更新日期：1991-03-01 00:00:00

abstract：:Several lichen species have been used for medicinal purposes throughout the ages, and they are reported to be effective in the treatment of different disorders including ulcer and cancer. It is revealed that lichens may be easily accessible sources of natural drugs and possible food supplements after their safety eval...

journal_title：Cytotechnology

authors： Turkez H,Aydin E,Aslan A

更新日期：2012-12-01 00:00:00

abstract：:Olive oil intake has been shown to induce significant levels of apoptosis in various cancer cells. These anti-cancer properties are thought to be mediated by phenolic compounds present in olive. These beneficial health effects of olive have been attributed, at least in part, to the presence of oleuropein and hydroxyty...

journal_title：Cytotechnology

authors： Han J,Talorete TP,Yamada P,Isoda H

更新日期：2009-01-01 00:00:00

abstract：:In the recent years, the possibility of utilizing extracellular vesicles for drug delivery purposes has been investigated in various models, suggesting that these vesicles may have such potential. In addition to the choice of donor cell type for vesicle production, a major obstacle still exists with respect of loading...

journal_title：Cytotechnology

authors： Johnsen KB,Gudbergsson JM,Skov MN,Christiansen G,Gurevich L,Moos T,Duroux M

更新日期：2016-10-01 00:00:00

abstract：:Mandibular molar anlages excised from 17-day mouse foetuses were cultured in vitro or in ovo (on the chorioallantoic membrane). In both cases, the explants were underlain either with a Millipore filter or with a piece of fibrin foam. Tooth germs were harvested after 7 days of cultivation and processed histologically. ...

journal_title：Cytotechnology

authors： Peterka M,Mandys V,Peterková R

更新日期：1991-09-01 00:00:00

abstract：:We screened for biological activity which induces neurite outgrowth in vitro from 300 species of marine algae from along the Japan coast for possible use as a treatment for the lack of neurotrophic factor which is considered to be a cause of Alzheimer's disease. In this study, we evaluated the neurite outgrowth promot...

journal_title：Cytotechnology

authors： Kamei Y,Sagara A

更新日期：2002-11-01 00:00:00

abstract：:To construct a recombinant protein highly producing cell lines, we have previously developed the Oncogene Activated Production (OAP) system by using BHK-21 cells. Here we verified the availability of the OAP system in CHO cells. We firstly generated 'primed' ras amplified CHO cells, ras clone I, by introducing human c...

journal_title：Cytotechnology

authors： Katakura Y,Seto P,Miura T,Ohashi H,Teruya K,Shirahata S

更新日期：1999-09-01 00:00:00

abstract：:In neuronal dendrites, septins localize to the base of the spine, a unique position which is sandwiched between the microtubule (MT)-rich dendritic shaft and the actin filament-rich spine. Here, we provide evidence for the association of SEPT6 with MTs in cultured rat hippocampal neurons. In normal cultures, SEPT6 clu...

journal_title：Cytotechnology

authors： Moon IS,Lee H,Walikonis RS

更新日期：2013-03-01 00:00:00

abstract：:Precision-cut liver tissue slices (PCLS) have been used for decades to study pharmacological metabolism as well as toxicology and efficacy of novel substances on primary material under standardized conditions. Slicing of primary liver tissue has been done using different slicing machines. Since there has been great va...

journal_title：Cytotechnology

authors： Zimmermann M,Lampe J,Lange S,Smirnow I,Königsrainer A,Hann-von-Weyhern C,Fend F,Gregor M,Bitzer M,Lauer UM

更新日期：2009-12-01 00:00:00

abstract：:The effect of oxygen partial pressure on viral replication was investigated with Vero/VSV system. At 10% oxygen partial pressure in spinner culture, VSV titer was significantly increased 130 fold compared to that obtained at 21%. A similar result was obtained for viral production in 1liter bioreactor. This implies tha...

journal_title：Cytotechnology

authors： Lim HS,Chang KH,Kim JH

更新日期：1999-11-01 00:00:00

abstract：:Electroporation is a way to induce nanometersized membrane pore for exogenous substances delivery into cytoplasm using an artificial electric field. Now it was widely used for molecules transfer especially in molecular experiments and genetic aspects. In recent years, modern electroporation on the embryo was developed...

journal_title：Cytotechnology

authors： Yuan TF

更新日期：2007-06-01 00:00:00

abstract：:The aim of this research was a comparative study on the isolation and culture of head kidney macrophages derived from Acipenser persicous and Rutilus frisii kutum as teleost and chondrostei species of fish. The macrophages were isolated by density gradient sedimentation, followed by adherence to a plastic surface. The...

journal_title：Cytotechnology

authors： Rastgar S,Movahedinia A,Salamat N,Salati A,Zabihi E

更新日期：2018-12-01 00:00:00