Abstract:
:Arrowroot (Maranta arundinacea. L) is an underutilized local crop potentially to be developed as carbohydrate source and functional food in Indonesia. The objectives of this research are to evaluate the immunostimulatory effects of arrowroot extracts in vitro by using animal cell culture techniques, and in vivo by using BALB/c mice. The arrowroot tuber extracts were prepared by heat-treatment at 121 °C for 20 min in distilled water. The IgM production stimulatory activity of arrowroot tuber extracts against human hybridoma HB4C5 cells and mouse splenocytes was assessed. The result indicated that the arrowroot tuber extract stimulated IgM production by HB4C5 cells and immunoglobulin (IgG, IgA and IgM) production by splenocytes in vitro. In addition, the arrowroot tuber extracts strongly enhanced interferon γ production by splenocytes. In vivo study indicated that the diet containing arrowroot extracts increased the serum IgG, IgA and IgM levels in mice. These results revealed that the arrowroot tuber extracts have immunostimulatory effects in vivo as well as in vitro.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Kumalasari ID,Harmayani E,Lestari LA,Raharjo S,Asmara W,Nishi K,Sugahara Tdoi
10.1007/s10616-011-9403-4subject
Has Abstractpub_date
2012-03-01 00:00:00pages
131-7issue
2eissn
0920-9069issn
1573-0778journal_volume
64pub_type
杂志文章相关文献
CYTOTECHNOLOGY文献大全abstract::This article describes the current status in the development of human monoclonal antibodies. Over the last ten years a lot of information about the human immune system has emerged. Combining these with the many new (bio-)technologies it is plausible that the long awaited breakthrough of this technology is close. This ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00365341
更新日期:1996-01-01 00:00:00
abstract::We describe possible functions of carbohydrates attached to growth factors and strategies to examine the functions, concentrating on erythropoietin, a major regulator of erythropoiesis. Erythropoietin in erythropoiesis functions as an endocrine hormone; it is produced by kidney cells and transferred into the circulati...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00744323
更新日期:1995-01-01 00:00:00
abstract::Chicken egg yolk lipoprotein (YLP) was partially fractionated into some constituents, and the effect of constituents of YLP were examined on the growth and immunoglobulin (IgM and IgG) secretion of a HB4C5 human-human hybridoma cell line cultured in serum-free medium. Among the fractions, YP-1 and YP-2 fractions (LDL-...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00749004
更新日期:1993-01-01 00:00:00
abstract::Understanding the growth dynamics of influenza viruses is an essential step in virus replication and cell-adaptation. The aim of this study was to elucidate the growth kinetic of a low pathogenic avian influenza H9N2 subtype in chicken embryo fibroblast (CEF) and chicken tracheal epithelial (CTE) cells during consecut...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-012-9495-5
更新日期:2013-05-01 00:00:00
abstract::The metabolism of hybridoma cells was controlled to reduce metabolic formation in fed-batch cultures by dynamically feeding a salt-free nutrient concentrate. For this purpose, on-line oxygen uptake rate (OUR) measurement was used to estimate the metabolic demand of hybridoma cells and to determine the feeding rate of ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1007945826228
更新日期:1997-07-01 00:00:00
abstract::We screened for biological activity which induces neurite outgrowth in vitro from 300 species of marine algae from along the Japan coast for possible use as a treatment for the lack of neurotrophic factor which is considered to be a cause of Alzheimer's disease. In this study, we evaluated the neurite outgrowth promot...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1023974323807
更新日期:2002-11-01 00:00:00
abstract::A fructose-based cell culture is suitable for the process control of protein production because of slow sugar consumption rate and low lactate accumulation. The fructose transporter, GLUT5, mediates its incorporation into cells and is required for the fructose-based culture. In order to produce efficiently recombinant...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-010-9289-6
更新日期:2010-08-01 00:00:00
abstract::Previous studies have demonstrated antidiabetic effects for rooibos (Aspalathus linearis) and aspalathin (ASP), one of its main polyphenols. Rooibos, an endemic plant of South Africa, is well-known for its use as herbal tea. Green ('unfermented') rooibos has been shown to contain more ASP than 'fermented' rooibos tea,...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-014-9816-y
更新日期:2015-08-01 00:00:00
abstract::The adult heart contains a population of cardiac progenitor cells (CPCs). Growing and collecting an adequate number of CPCs demands complex culture media containing growth factors. Since activated macrophages secrete many growth factors, we investigated if activated isolated heart cells seeded on a feeder layer of act...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-014-9814-0
更新日期:2016-08-01 00:00:00
abstract::Explant cultures of porcine coronary artery provided a coculture model, used as a paradigm of arterial wall in contact with vascular prosthesis which allowed the study of spatial and temporal changes in cell phenotype. First cells emerging from the explant had an endothelial phenotype monitored by cytoimmunostaining. ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1016195029359
更新日期:2001-09-01 00:00:00
abstract::This study describes two packed bed bioreactor configurations which were used to culture a mouse-mouse hybridoma cell line (ATCC HB-57) which produces an IgG1 monoclonal antibody. The first configuration consists of a packed column which is continuously perfused by recirculating oxygenated media through the column. In...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF02521730
更新日期:1992-01-01 00:00:00
abstract::Desorption electrospray ionization may be used as a fast and convenient method for analysis and identification of lipids in the cell culture. Oxidative stress, which usually involves changes in lipids, was used as a model of pathology to show the utility of this analysis methodology. This paper addresses the surface p...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-014-9734-z
更新日期:2015-12-01 00:00:00
abstract::We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum free media and suspension growth. Subsequent...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008021428969
更新日期:1998-11-01 00:00:00
abstract::The current study was conducted to determine whether there is a relation between hypertension and two different polymorphisms, including C1562T of the Matrix metalloproteinase-9 (MMP-9) gene and C677T of the methylenetetrahydrofolate reductase (MTHFR) gene. Genomic DNA obtained from 224 persons (125 patients with hype...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-013-9665-0
更新日期:2015-01-01 00:00:00
abstract::In this article, we describe a new method that facilitates to isolate mammalian cells inducible hyper-producing heterologous proteins. This method uses the tetracycline-inducible system to express both the selection marker and the heterologous gene, therefore, allows to increase the selection pressure by reducing the ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1023945517446
更新日期:2002-11-01 00:00:00
abstract::Carvacrol, the predominant monoterpene in many essential oils of Labitae including Origanum, Satureja, Thymbra, Thymus, and Corydothymus has substantial antibacterial, antifungal, antihelmintic, insecticial, analgesic and antioxidant activities. Approximately 75% of lung cancer is non-small cell carcinoma (NSCLC) whic...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/b:cyto.0000039917.60348.45
更新日期:2003-11-01 00:00:00
abstract::"In vitro" studies, commonly using porcine ovarian follicles, may generate inconsistent results when atretic follicles are not eliminated from the pool of experimental follicles. The present experiment was conducted to test the practical value of the macroscopie identification of large porcine follicles, which were as...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00758179
更新日期:1994-01-01 00:00:00
abstract::More than 35 years ago double Nobel laureate Linus Carl Pauling published a powerful model of the molecular mechanism of general anesthesia, generally referred to as the hydrate-microcrystal (aqueous-phase) theory. This hypothesis, based on the molecular behavior of water molecules, did not receive serious attention d...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-009-9216-x
更新日期:2009-04-01 00:00:00
abstract::The relatively recent discovery of miRNAs has added a completely new dimension to the study of the regulation of gene expression. The mechanism of action of miRNAs, the conservation between diverse species and the fact that each miRNA can regulate a number of targets and phenotypes clearly indicates the importance of ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-007-9049-4
更新日期:2007-04-01 00:00:00
abstract::A differentiation method of human bone marrow mesenchymal stem cells (MSCs) to chondrocytes was developed for the construction of a three-dimensional (3D) cartilage tissue. The adhesive cells, which were isolated from a human bone marrow aspirate were embedded in type I collagen in a poly-L: -lactate-glycolic acid cop...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-005-3751-x
更新日期:2005-01-01 00:00:00
abstract::Over the past decade, global interest in the development of therapeutic monoclonal antibodies (mAbs) has risen rapidly. As therapeutic agents, antibodies have shown marked efficacy in combatting a range of cancers and immune diseases with high target specificity and low toxicity (Carla Lucia et al. in PLoS ONE 6:e2407...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-020-00375-x
更新日期:2020-04-01 00:00:00
abstract::A simple protein free medium was formulated and tested in suspension culture using three hybridoma cell lines. The medium, referred to as CDSS (Chemically Defined Serum Substitutes), consisted of the basal medium DMEM:Ham F12, 1:1, with HEPES (D12H), plus pluronic F68, trace elements, ferric citrate, ascorbic acid, an...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF02215660
更新日期:1996-06-01 00:00:00
abstract::This study was undertaken to evaluate genotoxic potential of Thermopsis turcica aqueous extracts on the roots of onion bulb (Allium cepa L.) by comet assay and random amplified polymorphic DNA technique. The Allium root growth inhibition test indicated that the EC50 and 2×EC50 values were 8 and 16 mg/ml concentrations...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-014-9835-8
更新日期:2016-08-01 00:00:00
abstract::Although a variety of assays have been used to examine T cell responses in vitro, standardized ex vivo detection of antigen-specific CD4+ T cells from human circulatory PBMCs remains constrained by low-dimensional characterization outputs and the need for polyclonal, mitogen-induced expansion methods to generate detec...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-018-0222-8
更新日期:2018-10-01 00:00:00
abstract::Material and degree of reductance balance equations are used to estimate the rates of oxygen uptake and carbon dioxide evolution of animal cell cultures. Lumped compositions, molecular weight and reductance degree of cellular protein, monoclonal antibody, biomass and amino acid consumption (excluding glutamine and ala...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008004618163
更新日期:1999-05-01 00:00:00
abstract::The presence of a highly purified recombinant interleukin-2 (rIL-2) increased the production of immunoglobulin (IgM or IgG) by human-human hybridomas to 1.5-2.0 times the production by untreated cells. However, these cells did not react with anti-Tac (IL-2 receptor alpha) antibody. To enhance the response of the hybri...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00148808
更新日期:1990-07-01 00:00:00
abstract::Electroporation is a way to induce nanometersized membrane pore for exogenous substances delivery into cytoplasm using an artificial electric field. Now it was widely used for molecules transfer especially in molecular experiments and genetic aspects. In recent years, modern electroporation on the embryo was developed...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-007-9082-3
更新日期:2007-06-01 00:00:00
abstract::Primary rat hepatocytes dedifferentiate rapidly losing theactivities of the drug metabolising enzymes involved in thedetoxification of xenobiotics in the liver. An alternativeapproach to using primary hepatocytes for toxicity testing isthe development of immortalised hepatocyte cell lines via thetransfection of primar...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008159912634
更新日期:2000-10-01 00:00:00
abstract::Light scattering properties of hybridoma cells were examined with flow cytometry. Viable and dead cells form two distinct populations. The distribution of the two populations changes during a batch culture. the concentration of dead cells measured by flow cytometry correlates well to that measured by hemacytometer. Th...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00386140
更新日期:1989-05-01 00:00:00
abstract::We describe an organotypic model of human skin comprised of a stratified layer of human epidermal keratinocytes and dermal fibroblasts within a contracted collagen lattice. Feasible and reproducible production of the skin construct has required the use of traditional as well as specialized culture techniques. The conf...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF02521744
更新日期:1992-01-01 00:00:00