Abstract:
:Alphaviruses are positive stranded RNA viruses that replicate to extremely high titers. Sindbis and Semliki Forest viral vectors are widely used tools for high-level production of recombinant proteins. Recent studies have broadened their scope to vaccine production, gene therapy, and analysis of cell function. Here we discuss the development of non-cytopathic and inducible expression vectors which can be applied to bioprocess development strategies. Furthermore, a Sindbis-based expression cloning system has been developed that allows for the rapid identification of genes encoding proteins with a selected functional activity.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Boorsma M,Koller D,Renner WA,Bachmann MFdoi
10.1023/A:1013192017048subject
Has Abstractpub_date
2001-05-01 00:00:00pages
203-12issue
3eissn
0920-9069issn
1573-0778journal_volume
35pub_type
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