Primary adipocyte culture: adipocyte purification methods may lead to a new understanding of adipose tissue growth and development.


:In the present manuscript, the methods required to generate purified cultures of mature adipocytes, as well as stromal vascular cells, from the same isolation are detailed. Also, we describe the in vitro conditions for the dedifferentiation of the isolated mature adipocytes. These two types of cells may be used to reevaluate differences between presently available cellular models for lipogenesis/lipolysis and might provide a new cellular physiological system for studies utilizing the proliferative progeny from mature adipocyte dedifferentiation. Alternative possibilities to the dedifferentiation phenomenon are proposed, as this new area of research is novel.






Fernyhough ME,Vierck JL,Hausman GJ,Mir PS,Okine EK,Dodson MV




Has Abstract


2004-10-01 00:00:00












  • Development of human monoclonal antibodies: A review.

    abstract::This article describes the current status in the development of human monoclonal antibodies. Over the last ten years a lot of information about the human immune system has emerged. Combining these with the many new (bio-)technologies it is plausible that the long awaited breakthrough of this technology is close. This ...


    pub_type: 杂志文章


    authors: Lindl T

    更新日期:1996-01-01 00:00:00

  • Osteogenic differentiation of human placenta-derived mesenchymal stem cells (PMSCs) on electrospun nanofiber meshes.

    abstract::Numerous challenges remain in the successful clinical translation of cell-based therapeutic studies for skeletal tissue repair, including appropriate cell sources and viable cell delivery systems. Poly(ethylene glycol)-poly(ε-caprolactone) (PEG-PCL) amphiphilic block copolymers have been extensively explored in micros...


    pub_type: 杂志文章


    authors: Zhang D,Tong A,Zhou L,Fang F,Guo G

    更新日期:2012-12-01 00:00:00

  • Inhibition of hepatoma cell invasion beneath mesothelial-cell monolayer by sera from tea- and related component-treated rats and their modes of action.

    abstract::The bioavailability and action of teas on the invasion of a rat ascites hepatoma cell line, AH109A, were determined and their modes of action were by co-culturing the cancer cells with a rat mesentery-derived mesothelial-cell (M-cell) monolayer in the presence of sera from rats orally given teas and their component, (...


    pub_type: 杂志文章


    authors: Zhang G,Miura Y,Yagasaki K

    更新日期:2001-07-01 00:00:00

  • Doxorubicin and 5-fluorouracil resistant hepatic cancer cells demonstrate stem-like properties.

    abstract::The efficacy of hepatocellular carcinoma (HCC) treatment is very low because of the high percentage of recurrence and resistance to anticancer agents. Hepatic cancer stem cells (HCSCs) are considered the origin of such recurrence and resistance. Our aim was to evaluate the stemness of doxorubicin and 5-fluorouracil re...


    pub_type: 杂志文章


    authors: Vu NB,Nguyen TT,Tran LC,Do CD,Nguyen BH,Phan NK,Pham PV

    更新日期:2013-08-01 00:00:00

  • Dielectric spectroscopy of mammalian cells. 2. Simultaneous in situ evaluation by aperture impedance pulse spectroscopy and low frequency dielectric spectroscopy of the biomass of HTC cells on Cytodex 3.

    abstract::Low-frequency dielectric spectroscopy has been used in situ, i.e. while the cells are still attached to their microsupport, to monitor the changes of biomass accompanying the growth of anchorage-dependent cells. This method, when compared to Aperture Impedance Pulse Spectroscopy (also called electronic sizing), is cha...


    pub_type: 杂志文章


    authors: Degouys V,Cerckel I,Garcia A,Harfield J,Dubois D,Fabry L,Miller AO

    更新日期:1993-01-01 00:00:00

  • Chaperone and foldase coexpression in the baculovirus-insect cell expression system.

    abstract:CONCLUSIONS:The BEVS has become widely utilized for production of recombinant proteins. However, protein aggregation and inefficient processing often limit yields, especially for secreted and membrane proteins. Since many proteins of pharmaceutical interest require similar posttranslational processing steps, engineerin...


    pub_type: 杂志文章


    authors: Betenbaugh MJ,Ailor E,Whiteley E,Hinderliter P,Hsu TA

    更新日期:1996-01-01 00:00:00

  • Construction and characterization of a multilayered gingival keratinocyte culture model: the TURK-U model.

    abstract::In construction of epithelial cells as multilayers, the cells are grown submerged to confluence on fibroblast-embedded collagen gels and, then, lifted to air to promote their stratification. We recently demonstrated that gingival epithelial cells form uniform monolayers on semi-permeable nitrocellulose membranes, supp...


    pub_type: 杂志文章


    authors: Gursoy UK,Gursoy M,Könönen E,Sintim HO,Uitto VJ,Syrjänen S

    更新日期:2016-12-01 00:00:00

  • Modeling of cell culture processes.

    abstract::Models of cell processes can be particularly useful in simulating, optimizing and controlling cell culture systems. Models reported in the literature are of various degrees of biological structure and mathematical complexity and describe cell growth, death, metabolism, and product formation, alone or in combination wi...


    pub_type: 杂志文章,评审


    authors: Tziampazis E,Sambanis A

    更新日期:1994-01-01 00:00:00

  • Productivity enhancement of recombinant protein in CHO cells via specific promoter activation by oncogenes.

    abstract::To construct a recombinant protein highly producing cell lines, we have previously developed the Oncogene Activated Production (OAP) system by using BHK-21 cells. Here we verified the availability of the OAP system in CHO cells. We firstly generated 'primed' ras amplified CHO cells, ras clone I, by introducing human c...


    pub_type: 杂志文章


    authors: Katakura Y,Seto P,Miura T,Ohashi H,Teruya K,Shirahata S

    更新日期:1999-09-01 00:00:00

  • DESI-MS as a tool for direct lipid analysis in cultured cells.

    abstract::Desorption electrospray ionization may be used as a fast and convenient method for analysis and identification of lipids in the cell culture. Oxidative stress, which usually involves changes in lipids, was used as a model of pathology to show the utility of this analysis methodology. This paper addresses the surface p...


    pub_type: 杂志文章


    authors: Bodzon-Kulakowska A,Cichon T,Golec A,Drabik A,Ner J,Suder P

    更新日期:2015-12-01 00:00:00

  • Effect of new rotenoid glycoside from the fruits of Amorpha fruticosa LINNE on the growth of human immune cells.

    abstract::A new compound, rotenoid isoflavone glycoside named, 6'-O-beta-D: -glucopyranosyl-12a-hydroxydalpanol was isolated from the methanolic (MeOH) fruit extract of Amorpha fruticosa LINNE by means of multi-stage column chromatography. Immuno-modulatory activities of this new glycoside were compared with the partitioned fra...


    pub_type: 杂志文章


    authors: Lee HJ,Kang HY,Kim CH,Kim HS,Kwon MC,Kim SM,Shin IS,Lee HY

    更新日期:2006-11-01 00:00:00

  • A methoxyflavanone derivative from the Asian medicinal herb (Perilla frutescens) induces p53-mediated G2/M cell cycle arrest and apoptosis in A549 human lung adenocarcinoma.

    abstract::Perilla frutescens is an Asian dietary herb consumed as an essential seasoning in Japanese cuisine as well as used for a Chinese medicine. Here, we report that a newly found methoxyflavanone derivative from P. frutescens (Perilla-derived methoxyflavanone, PDMF; 8-hydroxy-5,7-dimethoxyflavanone) shows carcinostatic act...


    pub_type: 杂志文章


    authors: Abd El-Hafeez AA,Fujimura T,Kamei R,Hirakawa N,Baba K,Ono K,Kawamoto S

    更新日期:2018-06-01 00:00:00

  • MiR-548c-3p inhibits the proliferation, migration and invasion of human breast cancer cell by targeting E2F3.

    abstract::MiR-548 has been reported to be involved in a variety of tumor processes, but its function in breast cancer remains unclear. In this study, we found that miR-548 was low expressed in breast cancer tissues and cells compared with normal control. We then examined whether up-regulation of miR-548 could improve the progre...


    pub_type: 杂志文章


    authors: Tan PY,Wen LJ,Li HN,Chai SW

    更新日期:2020-10-01 00:00:00

  • Signs of deferasirox genotoxicity.

    abstract::Iron overload is a major health problem for patients who have to have continuous blood transfusions. It brings some metabolic problems together. Various iron chelating agents are being used for treatment of hemochromatosis which arises from excess iron accumulation. This study was conducted with the aim of determining...


    pub_type: 杂志文章


    authors: Ila HB,Topaktas M,Arslan M,Büyükleyla M

    更新日期:2014-08-01 00:00:00

  • Liver stem cells.

    abstract::The capacity of hepatocytes and cholangiocytes to contribute to their own maintenance has long been recognized. More recently, studies have indicated the presence of both intra-hepatic and extra-hepatic stem/progenitor cell populations. The intraorgan compartment probably derives primarily from the biliary tree, most ...


    pub_type: 杂志文章


    authors: Theise ND

    更新日期:2003-03-01 00:00:00

  • A modified preplate technique for efficient isolation and proliferation of mice muscle-derived stem cells.

    abstract::We modified an existing protocol to develop a more efficient method to acquire and culture muscle-derived stem cells (MDSCs) and compared the characteristics of cells obtained from the two methods. This method is based on currently used multistep enzymatic digestion and preplate technique. During the replating process...


    pub_type: 杂志文章


    authors: Xu Z,Yu L,Lu H,Feng W,Chen L,Zhou J,Yang X,Qi Z

    更新日期:2018-12-01 00:00:00

  • Cephalic hedgehog expression is regulated directly by Sox17 in endoderm development of Xenopus laevis.

    abstract::In early development of animals, hedgehog (Hh) genes function as morphogen in the axis determination and the organ formation. In Xenopus, three hedgehog genes, sonic (shh), banded (bhh), and cephalic (chh), were identified and might organize various tissues and organs in embryogenesis. Here, we report the spatial and ...


    pub_type: 杂志文章


    authors: Yagi Y,Ito Y,Kuhara S,Tashiro K

    更新日期:2008-06-01 00:00:00

  • Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells.

    abstract::The adult heart contains a population of cardiac progenitor cells (CPCs). Growing and collecting an adequate number of CPCs demands complex culture media containing growth factors. Since activated macrophages secrete many growth factors, we investigated if activated isolated heart cells seeded on a feeder layer of act...


    pub_type: 杂志文章


    authors: Sepúlveda DE,Cabeza Meckert P,Locatelli P,Olea FD,Pérez NG,Pinilla OA,Díaz RG,Crottogini A,Laguens RP

    更新日期:2016-08-01 00:00:00

  • β-mercaptoethanol promotes osteogenesis of human mesenchymal stem cells via sirt1-ERK pathway.

    abstract::Human umbilical cord-derived mesenchymal stem cells (hUMSCs) hold strong self-renewal capacity and low immunogenicity, which have attracted attention as potential candidates for bone repair and regeneration. However, insufficient osteogenic differentiation markedly hinders the clinical applications of hUMSCs. In the p...


    pub_type: 杂志文章


    authors: Liu J,Wang H,Ren W,Zhou Y,Ye Z,Tan WS

    更新日期:2020-10-01 00:00:00

  • Use of cell cultures, nuclear and radioanalytical techniques for metallotoxicological studies at the JRC-Ispra.

    abstract::This paper reviews the JRC-Ispra research activity on toxicological studies related to the environmental and occupational exposure of trace metals, as carried out by a combination of cell culture methodologies with nuclear and radioanalytical techniques. Applications concern the setting of uptake-effect relationships ...


    pub_type: 杂志文章


    authors: Sabbioni E,Pozzi G,Fischbach M,Bahbouth E

    更新日期:1993-01-01 00:00:00

  • Characterization of abalone Haliotis tuberculata-Vibrio harveyi interactions in gill primary cultures.

    abstract::The decline of European abalone Haliotis tuberculata populations has been associated with various pathogens including bacteria of the genus Vibrio. Following the summer mortality outbreaks reported in France between 1998 and 2000, Vibrio harveyi strains were isolated from moribund abalones, allowing in vivo and in vit...


    pub_type: 杂志文章


    authors: Pichon D,Cudennec B,Huchette S,Djediat C,Renault T,Paillard C,Auzoux-Bordenave S

    更新日期:2013-10-01 00:00:00

  • Nitric oxide synthesis inhibition and cytotoxicity of Korean horse mussel Modiolus modiolus extracts on cancer cells in culture.

    abstract::The Korean horse mussel extract was purified and fractionated by a bioassay-guided purification step. The final fraction contained seven steroid and one polycyclic aromatic compounds, in which cholest-7-en-3-ol, (3β,5α)- (58.7 %) was a main component followed by ergosta-7,22dien-3-ol (3β,5α,22E) (13.0 %). This extract...


    pub_type: 杂志文章


    authors: Wikarta JM,Kim SM

    更新日期:2016-08-01 00:00:00

  • DNA and cell death.

    abstract::The type of DNA damage and the role of poly (ADP-ribosyl) polymerase (ADPRP) and sulphated glyprotein 2 (SGP-2) in programmed cell death (apoptosis) was investigated in the following model systems: i) rat thymocytes treated with dexamethasone (DEX) eitherin vitro orin vivo; ii) human perypheral blood mononuclear cells...


    pub_type: 杂志文章


    authors: Monti D,Troiano L,Tropea F,Grassilli E,Cossarizza A,Barozzi D,Bettuzzi S,Corti A,Franceschi C

    更新日期:1991-02-01 00:00:00

  • Cloning of a new fibroblast cell line from an early primary culture from mandarin fish (Siniperca chuatsi) fry for efficient proliferation of megalocytiviruses.

    abstract::Megalocytiviruses are important emerging pathogens in both freshwater and marine finfish aquaculture. However, a limited number of piscine cell lines are persistently susceptible to these viruses, which greatly limits the study of megalocytiviruses. In this study, a new fibroblast-like cell line was established from a...


    pub_type: 杂志文章


    authors: Dong C,Shuang F,Weng S,He J

    更新日期:2014-12-01 00:00:00

  • Dental pulp stem cells and osteogenesis: an update.

    abstract::Dental pulp stem cells constitute an attractive source of multipotent mesenchymal stem cells owing to their high proliferation rate and multilineage differentiation potential. Osteogenesis is initiated by osteoblasts, which originate from mesenchymal stem cells. These cells express specific surface antigens that disap...


    pub_type: 杂志文章,评审


    authors: Mortada I,Mortada R

    更新日期:2018-10-01 00:00:00

  • Mouse single oocyte imaging by MALDI-TOF MS for lipidomics.

    abstract::Reproductive cells are a very special kind of material for the analysis. Depending on the species, their dimensions allow for the application of mass spectrometry imaging-based techniques to receive a reasonable data for interpretation of their condition without any additional sample preparation steps, except for typi...


    pub_type: 杂志文章


    authors: Bodzon-Kulakowska A,Arena R,Mielczarek P,Hartman K,Kozoł P,Gibuła-Tarlowska E,Wrobel TP,Gąsior Ł,Polański Z,Ptak GE,Suder P

    更新日期:2020-06-01 00:00:00

  • Different expression levels of TNF receptors on the rheumatoid synovial macrophages derived from surgery and a synovectomy as detected by a new flow cytometric analysis.

    abstract::TNFalpha plays a crucial role in the pathogenesis of rheumatoid arthritis. It is very important to examine the expression of the TNF receptors, the ligand of TNFalpha. In this study, we developed a triple-color flow cytometric analysis using CD45 and CD14 monoclonal antibodies to simply detect the expression of the TN...


    pub_type: 杂志文章


    authors: Ida H,Aramaki T,Nakamura H,Fujikawa K,Arima K,Tamai M,Kamachi M,Satoh K,Origuchi T,Kawakami A,Furuichi I,Kawabe Y,Eguchi K

    更新日期:2009-07-01 00:00:00

  • Small interfering RNA knockdown of mini-TyrRS and mini-TrpRS effects angiogenesis in human umbilical vein endothelial cells in hypoxic culture.

    abstract::Aim We studied the role of mini-TyrRS and mini-TrpRS in angiogenesis by using small interfering RNA-mediated mini-TyrRS/mini-TrpRS knockout in hypoxic culture of human umbilical vein endothelial cells. Methods SiRNA was used as the main method to inhibited the gene function. Silencing efficiency was assayed by real-ti...


    pub_type: 杂志文章


    authors: Zeng R,Chen YC,Zeng Z,Liu R,Qiang O,Jiang XF,Liu XX,Li X,Wang HY

    更新日期:2008-03-01 00:00:00

  • Epitope analysis of peanut allergen Ara h1 with human monoclonal IgM antibody 92-2.

    abstract::A human-mouse hybridoma clone 92-2 secreting IgM-class human monoclonal antibody to peanut allergen protein Ara h1 was established. To detect antibody-binding sequences on Ara h1, we synthesized a series of peptides of the Ara h1 protein on a multi-pin apparatus for the pin-peptide ELISA. The 92-2 human monoclonal ant...


    pub_type: 杂志文章


    authors: Shinmoto H,Matsuo Y,Naganawa Y,Tomita S,Takano-Ishikawa Y

    更新日期:2010-08-01 00:00:00

  • A continuous multistage roller reactor for animal cell culture: 1. Patterns of growth, production and catabolism of a murine hybridoma.

    abstract::A Tubular Liquid Film Reactor was designed as a model system to transfer a batch culture kinetic to a continuous cascade. Cell density, product formation and substrate consumption rates were followed during fermentation at two dilution rates. In spite of the high dilution rates effective in each segment by itself high...


    pub_type: 杂志文章


    authors: Borth N,Steindl F,Weigang F,Reiter M,Katinger H

    更新日期:1990-05-01 00:00:00