Primary adipocyte culture: adipocyte purification methods may lead to a new understanding of adipose tissue growth and development.

Abstract:

:In the present manuscript, the methods required to generate purified cultures of mature adipocytes, as well as stromal vascular cells, from the same isolation are detailed. Also, we describe the in vitro conditions for the dedifferentiation of the isolated mature adipocytes. These two types of cells may be used to reevaluate differences between presently available cellular models for lipogenesis/lipolysis and might provide a new cellular physiological system for studies utilizing the proliferative progeny from mature adipocyte dedifferentiation. Alternative possibilities to the dedifferentiation phenomenon are proposed, as this new area of research is novel.

journal_name

Cytotechnology

journal_title

Cytotechnology

authors

Fernyhough ME,Vierck JL,Hausman GJ,Mir PS,Okine EK,Dodson MV

doi

10.1007/s10616-005-2602-0

subject

Has Abstract

pub_date

2004-10-01 00:00:00

pages

163-72

issue

2-3

eissn

0920-9069

issn

1573-0778

journal_volume

46

pub_type

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