Effect of Partial Medium Replacement on Cell Growth and Protein Production for the High-Fivetrade mark insect cell line.


:The potential of spent medium to support the growth and recombinant protein production of High-Fivetrade mark cells was investigated. Growth in medium consisting of three parts fresh and one part spent medium was comparable to that in fresh medium (maximal specific growth rates of 0.028 and 0.029 h(-1), and maximal cell densities of 4 and 4.5 x 10(6) cells ml(-1), respectively). Glucose exhaustion coincided with an abrupt decrease of viability. Of 15 amino acids analyzed, not a single one was completely exhausted at the end of the growth phase. Growth in medium consisting of equal parts spent and fresh medium led to lower maximal cell concentration (2.9 x 10(6) cells ml(-1)) with a smoother death phase. Glucose supplementation at the beginning of the culture or at the end of the growth phase did not lead to an increase of either the maximal cell density or the specific growth rate. Infection of High-Fivetrade mark cells at three different densities (1.4, 2.5 and 4.2 x 10(6) cells ml(-1)) without medium change led to monotonically decreased specific productions for beta-galactosidase. Partial (75%) or total medium replacement at the higher infection density restored the specific production at the levels of the intermediate density infection (321, 292 and 389 U.(10(6) cells)(-1), respectively).






Ikonomou L,Bastin G,Schneider YJ,Agathos SN




Has Abstract


2004-01-01 00:00:00












  • The effect of glucose and glutamine on the intracellular nucleotide pool and oxygen uptake rate of a murine hybridoma.

    abstract::The effects of media concentrations of glucose andglutamine on the intracellular nucleotide pools andoxygen uptake rates of a murine antibody-secretinghybridoma cell line were investigated. Cells takenfrom mid-exponential phase of growth were incubated inmedium containing varying concentrations of glucose(0-25 mM) and...


    pub_type: 杂志文章


    authors: Barnabé N,Butler M

    更新日期:2000-10-01 00:00:00

  • Morphological observation and analysis using automated image cytometry for the comparison of trypan blue and fluorescence-based viability detection method.

    abstract::The ability to accurately determine cell viability is essential to performing a well-controlled biological experiment. Typical experiments range from standard cell culturing to advanced cell-based assays that may require cell viability measurement for downstream experiments. The traditional cell viability measurement ...


    pub_type: 杂志文章


    authors: Chan LL,Kuksin D,Laverty DJ,Saldi S,Qiu J

    更新日期:2015-05-01 00:00:00

  • Towards the development of a bioartificial pancreas: immunoisolation and NMR monitoring of mouse insulinomas.

    abstract::A promising method for diabetes treatment is the implantation of immunoisolated cells secreting insulin in response to glucose. Cell availability limits the application of this approach at a medically-relevant scale. We explore the use of transformed cells that can be grown to large homogeneous populations in developi...


    pub_type: 杂志文章


    authors: Sambanis A,Papas KK,Flanders PC,Long RC Jr,Kang H,Constantinidis I

    更新日期:1994-01-01 00:00:00

  • Evaluating the bottlenecks of recombinant IgM production in mammalian cells.

    abstract::Despite the fact, that monoclonal antibodies are the fastest growing group of biopharmaceuticals in development, this is not true for the IgM class, which remains as enigmatic as ever. While more examples of usefulness of IgMs for medical applications are emerging, their recombinant production is still not common. In ...


    pub_type: 杂志文章


    authors: Chromikova V,Mader A,Steinfellner W,Kunert R

    更新日期:2015-03-01 00:00:00

  • Real architecture For 3D Tissue (RAFT™) culture system improves viability and maintains insulin and glucagon production of mouse pancreatic islet cells.

    abstract::There is an unmet medical need for the improvement of pancreatic islet maintenance in culture. Due to restricted donor availability it is essential to ameliorate islet viability and graft engraftment. The aim of this study was to compare the standard tissue culture techniques with the advanced Real Architecture For 3D...


    pub_type: 杂志文章


    authors: Szebeni GJ,Tancos Z,Feher LZ,Alfoldi R,Kobolak J,Dinnyes A,Puskas LG

    更新日期:2017-04-01 00:00:00

  • Efficient production of recombinant IgG by metabolic control and co-expression with GLUT5 in a fructose-based medium.

    abstract::A fructose-based cell culture is suitable for the process control of protein production because of slow sugar consumption rate and low lactate accumulation. The fructose transporter, GLUT5, mediates its incorporation into cells and is required for the fructose-based culture. In order to produce efficiently recombinant...


    pub_type: 杂志文章


    authors: Inoue Y,Tsukamoto Y,Yamanaka M,Nakamura S,Inoue A,Nishino N,Kawahara H

    更新日期:2010-08-01 00:00:00

  • Identification of the genes specifically expressed in orally tolerized T cells.

    abstract::Oral tolerance is the systemic immunological unresponsiveness that occurs after feeding protein antigens. Its physiological role is thought to be the prevention of hypersensitivity to food antigens, and its therapeutic use to treat inflammatory diseases has been suggested. Although it has been shown that CD4(+) T cell...


    pub_type: 杂志文章


    authors: Gotoh T,Ise W,Nonaka A,Hamaguchi S,Hachimura S,Kaminogawa S

    更新日期:2003-11-01 00:00:00

  • Cloning of a new fibroblast cell line from an early primary culture from mandarin fish (Siniperca chuatsi) fry for efficient proliferation of megalocytiviruses.

    abstract::Megalocytiviruses are important emerging pathogens in both freshwater and marine finfish aquaculture. However, a limited number of piscine cell lines are persistently susceptible to these viruses, which greatly limits the study of megalocytiviruses. In this study, a new fibroblast-like cell line was established from a...


    pub_type: 杂志文章


    authors: Dong C,Shuang F,Weng S,He J

    更新日期:2014-12-01 00:00:00

  • Succinoyl trehalose lipid induced differentiation of human monocytoid leukemic cell line U937 into monocyte-macrophages.

    abstract::A novel type of succinoyl trehalose lipid (STL-1) prepared from n-hexadecane-culture of Rhodococcus erythropolis SD-74 markedly inhibited the growth of a human monocytoid leukemic cell line, U937, and induced its morphological alteration along a monocyte-macrophage lineage. STL-1 markedly increased differention-associ...


    pub_type: 杂志文章


    authors: Isoda H,Shinmoto H,Matsumura M,Nakahara T

    更新日期:1995-01-01 00:00:00

  • Anti-photoaging activities of Sorbaria kirilowii ethanol extract in UVB-damaged cells.

    abstract::Sorbaria kirilowii (Regel) Maxim, a plant found in China, Korea, Japan, and east of Europe, is a common herb used for traditional medicinal purposes. However, its ability to prevent photoaging has not been studied. In this study, we investigated the anti-photoaging functions of an ethanol extract (Sk-EE) of S. kirilow...


    pub_type: 杂志文章


    authors: Chen H,Jang J,Kopalli SR,Yum J,Yoon K,Cho JY

    更新日期:2021-02-01 00:00:00

  • Differentiation of Human Bone Marrow Mesenchymal Stem Cells to Chondrocytes for Construction of Three-dimensional Cartilage Tissue.

    abstract::A differentiation method of human bone marrow mesenchymal stem cells (MSCs) to chondrocytes was developed for the construction of a three-dimensional (3D) cartilage tissue. The adhesive cells, which were isolated from a human bone marrow aspirate were embedded in type I collagen in a poly-L: -lactate-glycolic acid cop...


    pub_type: 杂志文章


    authors: Matsuda C,Takagi M,Hattori T,Wakitani S,Yoshida T

    更新日期:2005-01-01 00:00:00

  • Aldehyde dehydrogenase activity of Wharton jelly mesenchymal stromal cells: isolation and characterization.

    abstract::Mesenchymal stromal cells (MSCs) are promising tools in regenerative medicine and targeted therapies. Although different origins have been described, there is still huge need to find a valuable source harboring specific subpopulations of MSCs with precise therapeutic functions. Here, we isolated by fluorescence activa...


    pub_type: 杂志文章


    authors: Najar M,Crompot E,van Grunsven LA,Dollé L,Lagneaux L

    更新日期:2019-02-01 00:00:00

  • The cell yields and biological characteristics of stromal/stem cells from lipoaspirate with different digestion loading ratio.

    abstract::Effective harvesting procedure for adipose tissue is demanded by the affordable Good Manufacturing Practice-Compliant Production of clinical-grade adipose tissue-derived stem cells (hADSCs). Enzymatic digestion using collagenase is the most reliable method of adipose tissue-derived stem cells (hADSCs) isolation, while...


    pub_type: 杂志文章


    authors: Li Z,Mu D,Liu C,Xin M,Fu S,Li S,Qi J,Wang Q,Luan J

    更新日期:2020-04-01 00:00:00

  • Versatile stem cells, young and old. A review.

    abstract::Both embryonic and somatic stem cells have been studied in recent years with particular regard to their differentiation potential. In vitro studies allow a considerable amplification of such cells in culture as well as the induction of commitment in different directions under proper stimulating factors. Moreover, a su...


    pub_type: 杂志文章


    authors: Eridani S

    更新日期:2001-03-01 00:00:00

  • Development of a Mammalian suspension culture for expression of active recombinant human urokinase-type plasminogen activator.

    abstract::The development of specific catalytic inhibitors for the serine protease urokinase-type plasminogen activator (uPA) has been hindered due to difficulties in producing sufficient amounts of active recombinant uPA that is catalytically equivalent to native uPA. The purpose of this study was to develop an efficient syste...


    pub_type: 杂志文章


    authors: Atkins E,Zamora S,Candia BJ,Baca A,Orlando RA

    更新日期:2005-09-01 00:00:00

  • Heat induction of reporter gene expression via the gadd153 promoter and its possible application to hyperthermia treatment of cancer.

    abstract::The use of the gadd153promoter to induce expression of a reporter geneunder heat stress conditions was investigated,since the results of previous studies have suggestedthat the gadd153promoter is likely to be activated by the indirecteffects of hyperthermia, that is, by DNA damage thatoccurs when reactive oxygen speci...


    pub_type: 杂志文章


    authors: Bouhon IA,Ito A,Shinkai M,Honda H,Kobayashi T

    更新日期:2000-07-01 00:00:00

  • High-level expression of a foreign gene by a recombinant baculovirus with an expanded host range.

    abstract::The usefulness of host range expanded viruses as an expressionvector system was investigated by following the expression ofthe E. coli lacZ gene. The host range expanded recombinantviruses were obtained from Sf-21 or BmN-4 cells coinfected withAutographa californica and Bombyx mori nuclearpolyhedrosis viruses. Among t...


    pub_type: 杂志文章


    authors: Kim HS,Woo SD,Kim WJ,Choi JY,Kang SK

    更新日期:2000-02-01 00:00:00

  • A plant extract which enhances the plating efficiency of lymphoid cell lines and enhances the survival of normal lymphoid cells in vitro.

    abstract::A water soluble extract from the bark of the Samoan medicinal plant Alphitonia zizyphoides A. Gray (Rhamnaceae), enhances the plating efficiency in vitro of lymphoid cell lines as well as the survival of bone marrow cells and normal T and B lymphocytes. Furthermore, the inclusion of bark-extract into culture media enh...


    pub_type: 杂志文章


    authors: Dunstan CA,Andersson J,Bohlin L,Cox PA,Grönvik KO

    更新日期:1994-01-01 00:00:00

  • Visible light regulates neurite outgrowth of nerve cells.

    abstract::The neurite outgrowth of PC12 cells on collagen-coated glass plates under light emitting diode (LED) irradiation at several wavelengths (i.e., 455, 470, 525, 600, 630, 880 and 945 nm) was investigated. No neurite outgrowth was observed during cultivation under irradiation from the lamp of an inverted light microscope ...


    pub_type: 杂志文章


    authors: Higuchi A,Watanabe T,Noguchi Y,Chang Y,Chen WY,Matsuoka Y

    更新日期:2007-07-01 00:00:00

  • Atorvastatin neutralises the thrombin-induced tissue factor expresion in endothelial cells via geranylgeranyl pyrophosphate.

    abstract::Statins may have beneficial effects in atherogenesis given their antithrombotic properties involving non-lipid mechanisms that modify endothelial function of tissue factor induction by thrombin. In this study, we investigate the effect of atorvastatin on tissue factor (TF) activity in thrombin-stimulated endothelial c...


    pub_type: 杂志文章


    authors: Martínez-Sales V,Vila V,Ferrando M,Reganon E

    更新日期:2011-01-01 00:00:00

  • A rat mammary gland cancer cell with stem cell properties of self-renewal and multi-lineage differentiation.

    abstract::The cancer stem cell hypothesis posits that tumors are derived from a single cancer-initiating cell with stem cell properties. The task of identifying and characterizing cancer-initiating cells with stem cell properties at the single cell level has proven technically difficult because of the scarcity of the cancer ste...


    pub_type: 杂志文章


    authors: Cocola C,Sanzone S,Astigiano S,Pelucchi P,Piscitelli E,Vilardo L,Barbieri O,Bertoli G,Reinbold RA,Zucchi I

    更新日期:2008-09-01 00:00:00

  • Evaluation of the antiviral effect of new compounds by using cellular cultures.

    abstract::Replication Kinetics of HIV was studied under single-growth cycle conditions in C8166 lymphoblastoid cells by back-titrating released and cell-associated infectious virus et each time point. Under these conditions HIV seemed to replicate faster than previously estimated. The amount cell-associated virus always exceede...


    pub_type: 杂志文章


    authors: Dianzani F,Antonelli G

    更新日期:1991-02-01 00:00:00

  • A continuous multistage roller reactor for animal cell culture: 1. Patterns of growth, production and catabolism of a murine hybridoma.

    abstract::A Tubular Liquid Film Reactor was designed as a model system to transfer a batch culture kinetic to a continuous cascade. Cell density, product formation and substrate consumption rates were followed during fermentation at two dilution rates. In spite of the high dilution rates effective in each segment by itself high...


    pub_type: 杂志文章


    authors: Borth N,Steindl F,Weigang F,Reiter M,Katinger H

    更新日期:1990-05-01 00:00:00

  • MiR-206 inhibits epilepsy and seizure-induced brain injury by targeting CCL2.

    abstract::To determine the function of miR-206 in epilepsy. Epileptic rat model was established by intra-amygdala injection of kainic acid (KA). Expression levels of miR-206, C-C Motif Chemokine Ligand 2 (CCL2) and interleukin-1β (Il-1β) in hippocampus tissues was measured by reverse transcription-quantitative PCR (RT-qPCR) and...


    pub_type: 杂志文章


    authors: Wu Z,Liu Y,Huang J,Huang Y,Fan L

    更新日期:2019-06-26 00:00:00

  • Effect of new rotenoid glycoside from the fruits of Amorpha fruticosa LINNE on the growth of human immune cells.

    abstract::A new compound, rotenoid isoflavone glycoside named, 6'-O-beta-D: -glucopyranosyl-12a-hydroxydalpanol was isolated from the methanolic (MeOH) fruit extract of Amorpha fruticosa LINNE by means of multi-stage column chromatography. Immuno-modulatory activities of this new glycoside were compared with the partitioned fra...


    pub_type: 杂志文章


    authors: Lee HJ,Kang HY,Kim CH,Kim HS,Kwon MC,Kim SM,Shin IS,Lee HY

    更新日期:2006-11-01 00:00:00

  • Mathematical models for multidrug resistance and its reversal.

    abstract::Mathematical models describing drug resistance are briefly reviewed. One model which describes the molecular function of the P-glycoprotein pump in multidrug resistant (MDR) cell lines has been developed and is presented in detail. The pump is modeled as an energy dependent facilitated diffusion process. A partial dif...


    pub_type: 杂志文章,评审


    authors: Michelson S

    更新日期:1993-01-01 00:00:00

  • Explants of porcine coronary artery in culture: A paradigm for studying the influence of heparin on vascular wall cell proliferation.

    abstract::Explant cultures of porcine coronary artery provided a coculture model, used as a paradigm of arterial wall in contact with vascular prosthesis which allowed the study of spatial and temporal changes in cell phenotype. First cells emerging from the explant had an endothelial phenotype monitored by cytoimmunostaining. ...


    pub_type: 杂志文章


    authors: Dufresne M,Warocquier-Clérout R

    更新日期:2001-09-01 00:00:00

  • Mouse single oocyte imaging by MALDI-TOF MS for lipidomics.

    abstract::Reproductive cells are a very special kind of material for the analysis. Depending on the species, their dimensions allow for the application of mass spectrometry imaging-based techniques to receive a reasonable data for interpretation of their condition without any additional sample preparation steps, except for typi...


    pub_type: 杂志文章


    authors: Bodzon-Kulakowska A,Arena R,Mielczarek P,Hartman K,Kozoł P,Gibuła-Tarlowska E,Wrobel TP,Gąsior Ł,Polański Z,Ptak GE,Suder P

    更新日期:2020-06-01 00:00:00

  • Comparison of RAP-PCR analysis of gene expression in fresh and immortalised rat hepatocyte cell lines.

    abstract::Primary rat hepatocytes dedifferentiate rapidly losing theactivities of the drug metabolising enzymes involved in thedetoxification of xenobiotics in the liver. An alternativeapproach to using primary hepatocytes for toxicity testing isthe development of immortalised hepatocyte cell lines via thetransfection of primar...


    pub_type: 杂志文章


    authors: Kelly HT,Hill E,Reid F,Grant MH,Macdonald C

    更新日期:2000-10-01 00:00:00

  • Optimization of cultivation conditions for monoclonal IgM antibody production by M1A2 hybridoma using artificial neural network.

    abstract::Monoclonal antibody (McAb) has been established as one of the most successful therapeutic strategies for the treatment of cancer. M1A2 (McAb) as a new monoclonal antibody was designed to recognize heat shock protein (HSP60), but its optimum production condition has not been studied. In this study, the cell culture con...


    pub_type: 杂志文章


    authors: Bashokouh F,Abbasiliasi S,Tan JS

    更新日期:2019-07-16 00:00:00