Abstract:
:We present a study of up-regulation of genes responsible for pancreatic development in glucose-sensitive insulin-secreting mesenchymal stem cells (IS-MSC) generated and differentiated from human adipose tissue (h-AD), with use of our specific differentiation media and without use of any xenogenic material. Anterior wall abdominal fat was collected from 56 volunteers and cultured in self-designed proliferation medium for 10 days. Cells were harvested by trypsinization and differentiated into insulin-expressing cells using self-designed differentiation medium for 3 days followed by evaluation for transcriptional factors Pax-6, Ipf-1, Isl-1, C-peptide and insulin secretion. Generated IS-MSC showed expression of Pax-6, Pdx-6 and Isl-1. Non-differentiated MSC as well as their further culture in absence of differentiation medium were used as negative controls. Generated 56 IS-MSC cell-lines were glucose responsive i.e. mean C-Peptide and insulin secretion levels were measured 0.41 ng/ml and 13.13 μU/ml, respectively, in absence of glucose which rose to 1.18 ng/ml and 83.42 μU/ml, respectively, following glucose challenge (p < 0.001). The mean rise in C-peptide and insulin secretion levels was 2.88 and 6.35 fold, respectively. To conclude insulin-secreting h-AD-MSC can be generated safely and effectively with application of specific differentiation media without xenogeneic material/any genetic modification, showing expression of transcriptional factors Pax-6, Ipf-1 and Isl-1.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Dave SD,Vanikar AV,Trivedi HLdoi
10.1007/s10616-013-9573-3subject
Has Abstractpub_date
2014-03-01 00:00:00pages
299-307issue
2eissn
0920-9069issn
1573-0778journal_volume
66pub_type
杂志文章相关文献
CYTOTECHNOLOGY文献大全abstract::In the recent years, the possibility of utilizing extracellular vesicles for drug delivery purposes has been investigated in various models, suggesting that these vesicles may have such potential. In addition to the choice of donor cell type for vesicle production, a major obstacle still exists with respect of loading...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-016-9952-7
更新日期:2016-10-01 00:00:00
abstract::ADG cell line was established from an abalonedigestive gland and previously characterized. ADGcells have the potential to grow in protein-freeculture and secrete l3 types of glycosidases. Inthis article, we determined the origin of ADG cell line,using electron microscopy, and purified a glycosidasesecreted by these ce...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008175226819
更新日期:2000-07-01 00:00:00
abstract::The Korean horse mussel extract was purified and fractionated by a bioassay-guided purification step. The final fraction contained seven steroid and one polycyclic aromatic compounds, in which cholest-7-en-3-ol, (3β,5α)- (58.7 %) was a main component followed by ergosta-7,22dien-3-ol (3β,5α,22E) (13.0 %). This extract...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-014-9840-y
更新日期:2016-08-01 00:00:00
abstract::Lymphoid cells expressing sufficient levels of Bcl-2 or E1B-19K are known to resist to induction of apoptosis in glutamine-free or nutrient-limited batch cultures. However, despite the increased viability and prolonged stationary phase achieved in batch culture, product yields are not necessarily improved. Here we hav...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008054403470
更新日期:1998-11-01 00:00:00
abstract::We provide evidence that the expression of the human MRP/GS-X pump encoded by the MRP (multidrug resistance associated protein) gene is induced by cisplatin in human leukemia HL-60/R-CP (cisplatin-resistant) cells and modulates cell growth inhibition by delta(7)-prostaglandin A1 (PGA1) methyl ester. The MRP mRNA level...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00744216
更新日期:1996-01-01 00:00:00
abstract::Toxicity testing is required for new chemicals being introduced onto the market. The use of animals in evaluating chemical safety is costly and time consuming. Furthermore, there is the ethical need to develop alternative methods to reduce the required number of animals. The new in vitro assays offer numerous advantag...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::To explore the role of ribosomal protein S15A (RPS15A) in breast cancer. The Oncomine database was used to compare the expression of RPS15A in human breast cancer tissues and normal tissues. RPS15A in breast cancer cell line ZR-75-30 and BT474 was specifically knocked down using lentivirus-mediated short hairpin RNAs ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-018-0221-9
更新日期:2018-10-01 00:00:00
abstract::Fishes have been recently recognized as a suitable model organism to study vertebrate physiological processes, in particular skeletal development and tissue mineralization. However, there is a lack of well characterized in vitro cell systems derived from fish calcified tissues. We describe here a protocol that was suc...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-007-9098-8
更新日期:2007-09-01 00:00:00
abstract::The type of DNA damage and the role of poly (ADP-ribosyl) polymerase (ADPRP) and sulphated glyprotein 2 (SGP-2) in programmed cell death (apoptosis) was investigated in the following model systems: i) rat thymocytes treated with dexamethasone (DEX) eitherin vitro orin vivo; ii) human perypheral blood mononuclear cells...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00736814
更新日期:1991-02-01 00:00:00
abstract::In early development of animals, hedgehog (Hh) genes function as morphogen in the axis determination and the organ formation. In Xenopus, three hedgehog genes, sonic (shh), banded (bhh), and cephalic (chh), were identified and might organize various tissues and organs in embryogenesis. Here, we report the spatial and ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-008-9127-2
更新日期:2008-06-01 00:00:00
abstract::Desorption electrospray ionization may be used as a fast and convenient method for analysis and identification of lipids in the cell culture. Oxidative stress, which usually involves changes in lipids, was used as a model of pathology to show the utility of this analysis methodology. This paper addresses the surface p...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-014-9734-z
更新日期:2015-12-01 00:00:00
abstract::Several lichen species have been used for medicinal purposes throughout the ages, and they are reported to be effective in the treatment of different disorders including ulcer and cancer. It is revealed that lichens may be easily accessible sources of natural drugs and possible food supplements after their safety eval...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-012-9447-0
更新日期:2012-12-01 00:00:00
abstract::By employing the affinity gel fraction technique, we have detected a 175 kDa tyrosine-O-sulfate (TyrS)-binding protein in sodium choleate extracts of the microsomal membrane fractions of bovine liver and pancreas, as well as canine liver and pancreas. Western blot analysis revealed the presence of the bovine liver Tyr...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1007903315687
更新日期:1997-01-01 00:00:00
abstract::Medium-chain fatty acids (MCFAs) have been suggested as an alternative to the use of antibiotics in animal nutrition with promising results. First, we studied the sensitivity of Salmonella Enteritidis and an enteropathogenic Escherichia coli strain against caprylic (C8), capric (C10) and lauric (C12) acids. A porcine ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-016-0003-1
更新日期:2016-10-01 00:00:00
abstract::We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum free media and suspension growth. Subsequent...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008021428969
更新日期:1998-11-01 00:00:00
abstract::Early developments in serum-free media led to a variety of formulations in which components normally provided in serum and required for growth (insulin, transferrin, lipid supplements, trace elements) and poorly defined components (extracts, hydrolysates) were added to defined basal media. These additives were mostly ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-006-9002-y
更新日期:2006-03-01 00:00:00
abstract::The aim of the study was to obtain the highest number of multipotent adipose-derived mesenchymal stem cells (ADMSCs) by using culture conditions which favour cell expansion without loss of mesenchymal stem cells (MSC)-like properties. Based on the assumption that stem cells reside in niches characterized by hypoxic co...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-014-9731-2
更新日期:2015-12-01 00:00:00
abstract::We screened for biological activity which induces neurite outgrowth in vitro from 300 species of marine algae from along the Japan coast for possible use as a treatment for the lack of neurotrophic factor which is considered to be a cause of Alzheimer's disease. In this study, we evaluated the neurite outgrowth promot...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1023974323807
更新日期:2002-11-01 00:00:00
abstract::Material and degree of reductance balance equations are used to estimate the rates of oxygen uptake and carbon dioxide evolution of animal cell cultures. Lumped compositions, molecular weight and reductance degree of cellular protein, monoclonal antibody, biomass and amino acid consumption (excluding glutamine and ala...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008004618163
更新日期:1999-05-01 00:00:00
abstract::Bauhinia holophylla (Bong.) Steud. (Fabaceae) is a plant used in Brazilian folk medicine to treat diabetes and inflammation. This study evaluated the phytochemical properties, cytotoxic, apoptotic, mutagenic/antimutagenic effects and alterations in gene expression (RNAm) in HepG2 cells treated with the B. holophylla e...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-017-0173-5
更新日期:2018-04-01 00:00:00
abstract::Vasculogenesis, the formation of blood vessels in embryonic or fetal tissue mediated by immature vascular cells (i.e., angioblasts), is poorly understood. Here we report a summary of our recent studies on the identification of a population of vascular progenitor cells (VPCs) in human fetal aorta. These undifferentiate...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-008-9167-7
更新日期:2008-09-01 00:00:00
abstract::This study describes two packed bed bioreactor configurations which were used to culture a mouse-mouse hybridoma cell line (ATCC HB-57) which produces an IgG1 monoclonal antibody. The first configuration consists of a packed column which is continuously perfused by recirculating oxygenated media through the column. In...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF02521730
更新日期:1992-01-01 00:00:00
abstract::We developed a simple method for freezing anchorage-dependent cells, including primary cultured rat liver parenchymal cells, without detaching the cells from the culture dish. The method consists of preculture of the cells to confluence, changing the growth medium to a conventional freezing medium, packaging in a cont...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00556297
更新日期:1991-03-01 00:00:00
abstract::Statins may have beneficial effects in atherogenesis given their antithrombotic properties involving non-lipid mechanisms that modify endothelial function of tissue factor induction by thrombin. In this study, we investigate the effect of atorvastatin on tissue factor (TF) activity in thrombin-stimulated endothelial c...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-010-9319-4
更新日期:2011-01-01 00:00:00
abstract::The objective of this study was to compare effects of in vitro culture systems on embryonic development and expression patterns of developmentally important genes in preimplantation buffalo embryos. After IVM/IVF presumptive zygotes were cultured in one of three systems: undefined TCM-199, mCR2aa medium supplemented w...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-016-0010-2
更新日期:2016-10-01 00:00:00
abstract::In the present study, we isolated preadipocytes from the adipose tissue of Peking duck and subsequently cultured them in vitro. Cell counting kit-8 assay was employed to establish the growth curve of duck primary preadipocytes. Meanwhile, after the cells reaching full confluency, they were induced to differentiate int...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-014-9715-2
更新日期:2015-10-01 00:00:00
abstract::Although the insect cell/baculovirus system is an important expression platform for recombinant protein production, our understanding of insect cell metabolism with respect to enhancing cell growth capability and productivity is still limited. Moreover, different host insect cell lines may have different growth charac...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-013-9637-4
更新日期:2014-10-01 00:00:00
abstract::Human herpes virus 6 (HHV-6) is a common human pathogen that is most often detected in hematopoietic cells. Although human cells harboring chromosomally integrated HHV-6 can be generated in vitro, the availability of such cell lines originating from in vivo tissues is limited. In this study, chromosomally integrated H...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-017-0119-y
更新日期:2018-02-01 00:00:00
abstract::Daphnoretin is a bicoumarin compound isolated from a natural product, Wikstroemia indica, which has been used to treat many diseases. It has strong antiviral and anti-tumor activities. Taking the anti-tumor activity of daphnoretin as a starting point, the present study aimed to test the pro-apoptotic effect of daphnor...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-013-9536-8
更新日期:2014-01-01 00:00:00
abstract::Dental pulp stem cells constitute an attractive source of multipotent mesenchymal stem cells owing to their high proliferation rate and multilineage differentiation potential. Osteogenesis is initiated by osteoblasts, which originate from mesenchymal stem cells. These cells express specific surface antigens that disap...
journal_title:Cytotechnology
pub_type: 杂志文章,评审
doi:10.1007/s10616-018-0225-5
更新日期:2018-10-01 00:00:00