Abstract:
:Fusion of lymphoblastoid cell lines that produce human monoclonal antibodies against Pseudomonas aeruginosa with the human/mouse heteromyeloma SHM-D33 generated heterohybrids that were stable and secreted antibody in the range of 20 to 300 micrograms/ml. One of the hybridoma cell lines ws adapted to serum-free medium and maintained for 60 days in an automated hollow fiber system. During that time, 3 g of antibody was produced. Such yields make it possible to evaluate these monoclonals for their therapeutic potential in patients at risk for Pseudomonas infections.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Gammon MC,Banas MH,Boccumini LE,Sigal NH,Zweerink HJdoi
10.1007/BF00365266subject
Has Abstractpub_date
1990-01-01 00:00:00pages
51-60issue
1eissn
0920-9069issn
1573-0778journal_volume
3pub_type
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