Heterohybridomas that secrete high levels of pseudomonas-specific therapeutic human monoclonal antibodies: their generation and large scale growth in an automated hollow fiber cell culture system.

Abstract:

:Fusion of lymphoblastoid cell lines that produce human monoclonal antibodies against Pseudomonas aeruginosa with the human/mouse heteromyeloma SHM-D33 generated heterohybrids that were stable and secreted antibody in the range of 20 to 300 micrograms/ml. One of the hybridoma cell lines ws adapted to serum-free medium and maintained for 60 days in an automated hollow fiber system. During that time, 3 g of antibody was produced. Such yields make it possible to evaluate these monoclonals for their therapeutic potential in patients at risk for Pseudomonas infections.

journal_name

Cytotechnology

journal_title

Cytotechnology

authors

Gammon MC,Banas MH,Boccumini LE,Sigal NH,Zweerink HJ

doi

10.1007/BF00365266

subject

Has Abstract

pub_date

1990-01-01 00:00:00

pages

51-60

issue

1

eissn

0920-9069

issn

1573-0778

journal_volume

3

pub_type

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