abstract：:Hepatitis A virus (HAV) is mainly transmitted via contaminated food or water or through person-to-person contact. Here, we describe development and evaluation of a reverse transcription droplet digital PCR (RT-ddPCR) and reverse transcription real-time PCR (RT-qPCR) assay for detection of HAV in food and clinical spec...

journal_title：Journal of virological methods

authors： Persson S,Alm E,Karlsson M,Enkirch T,Norder H,Eriksson R,Simonsson M,Ellström P

更新日期：2021-02-01 00:00:00

abstract：:Large-scale serosurveillance of severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) will only be possible if serological tests are sufficiently reliable, rapid and affordable. Many assays are either labour-intensive and require specialised facilities (e.g. virus neutralization assays), or are expensive w...

journal_title：Journal of virological methods

authors： Mariën J,Ceulemans A,Michiels J,Heyndrickx L,Kerkhof K,Foque N,Widdowson MA,Mortgat L,Duysburgh E,Desombere I,Jansens H,Van Esbroeck M,Ariën KK

更新日期：2021-02-01 00:00:00

abstract：:Recently, immunoblots (IBs) have tended to substitute Western blots (WBs) for HIV infection diagnosis. Several studies have confirmed IBs' high sensitivity to confirm HIV infection for every stage. Since the nature and pattern of the antigens of IBs are different from those of WB, the abilities of IBs and WBs to disti...

journal_title：Journal of virological methods

authors： Mariaggi AA,Gardiennet E,Stefic K,Essat A,Cheret A,Goujard C,Meyer L,Barin F,Avettand-Fenoel V,ANRS PRIMO cohort.

更新日期：2021-01-21 00:00:00

abstract：:A duplex SYBR Green I-based real-time PCR assay was established for the simultaneous detection of canine kobuvirus (CaKoV) and canine astrovirus (CaAstV). This assay can easily distinguish the two viruses according to their different melting temperatures (Tm) of 80 °C for CaKoV and 86.5 °C for CaAstV; other canine ent...

journal_title：Journal of virological methods

authors： Wang Y,Li Y,Cui Y,Jiang S,Liu H,Wang J,Li Y

更新日期：2021-01-13 00:00:00

abstract：BACKGROUND:Diagnostic real time reverse transcription PCR (rRT-PCR) is usually done using nucleic acid (NA) purified from the sample. In the SARS-CoV-2 pandemic reagents and utensils for NA purification has been in short supply. This has generated interest in methods that eliminate the need for NA purification. OBJECT...

journal_title：Journal of virological methods

authors： Jørgensen RL,Pedersen MS,Chauhan AS,Andreasson LM,Kristiansen GQ,Lisby JG,Rosenstierne MW,Schønning K

更新日期：2021-01-08 00:00:00

abstract：:The pandemic caused by SARS-CoV-2 has triggered an extraordinary collapse of healthcare systems and hundred thousand of deaths worldwide. Following the declaration of the outbreak as a Public Health Emergency of International Concern by the World Health Organization (WHO) on January 30th, 2020, it has become imperativ...

journal_title：Journal of virological methods

authors： Pereira-Gómez M,Fajardo Á,Echeverría N,López-Tort F,Perbolianachis P,Costábile A,Aldunate F,Moreno P,Moratorio G

更新日期：2020-12-04 00:00:00

abstract：:African Swine Fever Virus (ASFV) causes a transmissible and fatal disease in pigs that is currently devastating global swine production. Efficient and economical collection of genetic data from ASFV field isolates is essential for bio-surveillance, to limit and control its spread, and to better understand ASF disease ...

journal_title：Journal of virological methods

authors： Meekins DA,Trujillo JD,Gaudreault NN,Morozov I,Pérez-Núñez D,Revilla Y,Richt JA

更新日期：2020-11-01 00:00:00

abstract：:Inflammasome plays a major role in innate immune responses by activating caspase-1, resulting in secretion of interleukin-1β (IL-1β) and inflammatory pathologic responses. IL-1β release is widely used as an indirect readout to study inflammasome activation. Here we report an iGLuc reporter (pro-IL-1β-Gluc) of pig orig...

journal_title：Journal of virological methods

authors： Song J,Li K,Li T,Zhao G,Zhou S,Li H,Li J,Weng C

更新日期：2020-11-01 00:00:00

abstract：:Lumpy skin disease virus (LSDV) is a poxvirus that causes systemic disease in cattle, resulting in substantial economic loss to affected communities. LSDV is a rapidly emerging pathogen of growing global concern that recently spread from Africa and the Middle East into Europe and Asia, impacting the cattle population ...

journal_title：Journal of virological methods

authors： Fay PC,Cook CG,Wijesiriwardana N,Tore G,Comtet L,Carpentier A,Shih B,Freimanis G,Haga IR,Beard PM

更新日期：2020-11-01 00:00:00

abstract：:Among the enteric viruses implicated in waterborne outbreaks, human norovirus and hepatitis A virus (HAV) are a serious public health issue. Most foodborne viruses are difficult or currently unlikely to cultivate. Because of the lack of a cell culture method, real-time reverse transcriptase PCR is commonly used for th...

journal_title：Journal of virological methods

authors： Hennechart-Collette C,Dehan O,Fraisse A,Martin-Latil S,Perelle S

更新日期：2020-10-01 00:00:00

abstract：:The clinical and epidemiologic management of the SARS-CoV-2 pandemic is critically dependent on molecular assays with short turn-around time. We validated the novel Xpert Xpress SARS-CoV-2 assay using a commercial nucleic acid testing (Roche Cobas 6800). We found an excellent concordance over a range of SARS-CoV-2 loa...

journal_title：Journal of virological methods

authors： Goldenberger D,Leuzinger K,Sogaard KK,Gosert R,Roloff T,Naegele K,Cuénod A,Mari A,Seth-Smith H,Rentsch K,Hinić V,Hirsch HH,Egli A

更新日期：2020-10-01 00:00:00

abstract：:A chimeric porcine circovirus (PCV) 1-2b vaccine strain and its parental wild-type PCV2b strain from China (PCV2-J) were used separately to vaccinate BALB/c mice and tissue and serum samples were collected from the mice to investigate whether the replication properties of the viruses differed. The spleen lymphocytes f...

journal_title：Journal of virological methods

authors： Cui X,Wang X,Gao Q,Liu X,Kai Y,Chen C,Gao S

更新日期：2020-09-01 00:00:00

abstract：:Following the introduction of African swine fever virus (ASFV) into Europe in 2007, ASFV infection has spread continuously over the past years and it became a high level disease threat in Europe and also Asia. Examination of suspect clinical cases for ASF with rapid and sensitive laboratory methods can substantially c...

journal_title：Journal of virological methods

authors： Szeredi L,Bakcsa E,Zádori Z,Mészáros I,Olasz F,Bálint Á,Locsmándi G,Erdélyi K

更新日期：2020-05-23 00:00:00

abstract：:Canine Distemper Virus (CDV) is a highly contagious pathogen of dogs that causes severe respiratory, gastrointestinal and nervous signs. Although vaccines have been used to prevent infections, CDV has been reported worldwide, even in vaccinated animals. In the present study, a representative wild type CDV strain (Arg2...

journal_title：Journal of virological methods

authors： Romanutti C,Keller L,La Torre J,Panzera Y,Fuques E,Pérez R,Gallo Calderon M

更新日期：2020-05-01 00:00:00

abstract：OBJECTIVE:This study evaluated the performance of the cobas® hepatitis C virus (HCV) Test for use on the cobas® 6800/8800 Systems for the detection and quantification of HCV RNA collected using the cobas® Plasma Separation Card (PSC) compared with plasma samples. METHODS:Whole EDTA-venous blood was collected from 50 H...

journal_title：Journal of virological methods

authors： Marins EG,Krey N,Becker A,Melzer S,Hoppler M

更新日期：2020-04-01 00:00:00

abstract：:Latent fruit tree viruses present economic threat to the industry and nurseries as diseases they cause not only reduce fruit quality and production yield, but can also be spread inadvertently through propagation due to the lack of viral symptoms on an infected mother plant. As a result, these viruses require appropria...

journal_title：Journal of virological methods

authors： Beaver-Kanuya E,Harper SJ

更新日期：2020-04-01 00:00:00

abstract：:The nucleocapsid (N) protein of a coronavirus plays a crucial role in virus assembly and in its RNA transcription. It is important to characterize a virus at the nucleotide level to discover the virus's genomic sequence variations and similarities relative to other viruses that could have an impact on the functions of...

journal_title：Journal of virological methods

authors： Sheikh A,Al-Taher A,Al-Nazawi M,Al-Mubarak AI,Kandeel M

更新日期：2020-03-01 00:00:00

abstract：:Long-term viral archives are valuable sources of research data. Each archive can store hundreds of thousands of diverse sample types. In the current era of whole genome sequencing, archived samples become a rich source of evolutionary and epidemiological data that can span years, and even decades. However, the ability...

journal_title：Journal of virological methods

authors： Hopken MW,Piaggio AJ,Pabilonia KL,Pierce J,Anderson T,Abdo Z

更新日期：2020-02-01 00:00:00

abstract：:The mosaic disease caused by fig mosaic virus (FMV) is considered the plague of fig worldwide. A naïve phage display library, raised against the recombinant nucleocapsid protein of FMV (FMV-Np) was screened to obtain specific monoclonal recombinant antibodies in the form of single chain variable fragments (scFvs). Aft...

journal_title：Journal of virological methods

authors： Shahmirzaie M,Safarnejad MR,Rakhshandehroo F,Safarpour H,Shirazi FH,Zamanizadeh HR,Elbeaino T

更新日期：2020-02-01 00:00:00

abstract：:Ensuring the successful inactivation of select agent material is critical for maintaining compliance with federal regulations and safeguarding laboratory personnel from exposure to dangerous pathogens. Rift Valley fever virus (RVFV), naturally transmitted by mosquitoes, is classified as a select agent by the CDC and U...

journal_title：Journal of virological methods

authors： Bergren NA,Patterson EI,Blair H,Ellis RP,Kading RC

更新日期：2020-02-01 00:00:00

abstract：:Peste des petits ruminants (PPR) is a viral disease of small ruminants that is caused by the PPR virus (PPRV) and is a significant burden on subsistence farmers across the developing world. Loop-mediated isothermal amplification (LAMP) provides cost-effective, rapid, specific and sensitive detection of nucleic acid an...

journal_title：Journal of virological methods

authors： Rajko-Nenow P,Flannery J,Arnold H,Howson ELA,Darpel K,Stedman A,Corla A,Batten C

更新日期：2019-12-01 00:00:00

abstract：:A molecular diagnostic assay for the rapid, sensitive and specific detection of Apple stem pitting virus (ASPV) in infected samples, utilizing reverse transcription-recombinase polymerase amplification (RT-RPA) at an isothermal constant temperature of 42 °C and the designed target-specific primers, was developed. The ...

journal_title：Journal of virological methods

authors： Kim NY,Lee HJ,Jeong RD

更新日期：2019-12-01 00:00:00

abstract：:Giant salamander iridovirus (GSIV) belongs to the epizootic genus Ranavirus, and is the cause of epidemic diseases associated with high mortality and great losses to artificial breeding and farming. Here, we established a simple, accurate, and reliable cross-priming amplification (CPA) method to detect GSIV. The CPA a...

journal_title：Journal of virological methods

authors： Zhang H,Wang F,Jia R,Fan J,Hao J,Du J,Niu Y,Han S,Deng R,Zhang G

更新日期：2019-12-01 00:00:00

abstract：:In 2013, a unique seventh yellow head virus genotype (YHV7) was detected in Black Tiger shrimp (Penaeus monodon) broodstock that suffered high mortality following their capture from Joseph Bonaparte Gulf (JBG) in northern Australia. To assist with its diagnosis and assessment of its distribution, prevalence and pathog...

journal_title：Journal of virological methods

authors： Cowley JA,Rao M,Mohr P,Moody NJ,Sellars MJ,Crane MS

更新日期：2019-11-01 00:00:00

abstract：BACKGROUND:Influenza causes a significant annual disease burden, with characterization of the infecting virus important in clinical and public health settings. Rapid immunoassays are fast but insensitive, whereas real-time RT-PCR is sensitive but susceptible to genetic mutations and often requires multiple serial assay...

journal_title：Journal of virological methods

authors： Taylor AW,Dawson ED,Blair RH,Johnson JE Jr,Slinskey AH,Smolak AW,Toth E,Liikanen K,Stoughton RS,Smith C,Talbot S,Rowlen KL

更新日期：2019-11-01 00:00:00

abstract：:The emergence of novel and divergent HoBi-like pestivirus (HoBiPeV) strains in cattle in Asia recently has raised concerns with regard to their reliable and accurate diagnosis. Hence, the aim of this study was to evaluate currently available BVDV diagnostic tests and HoBiPeV-specific diagnostic tests in detection of g...

journal_title：Journal of virological methods

authors： Moorthy D,Mishra N,Kalaiyarasu S,Jhade SK,Singh VP

更新日期：2019-10-01 00:00:00

abstract：:Pollen transmitted viruses require accurate detection and identification to minimize the risk of spread through the global import and export of pollen. Therefore in this study we developed RT-qPCR assays for the detection of Cherry leaf roll virus (CLRV), Prune dwarf virus (PDV), Prunus necrotic ringspot virus (PNRSV)...

journal_title：Journal of virological methods

authors： Beaver-Kanuya E,Harper SJ

更新日期：2019-09-01 00:00:00

abstract：:CD4 T-cell count is a priority for staging HIV disease and guiding clinical management as part of HIV care. Conventional CD4 T-cell enumeration methods based on flow cytometry are expensive, require well-trained personnel, and are challenging to use in rural, resource-scarce areas. A simple CD4 T-cell count test that ...

journal_title：Journal of virological methods

authors： Bystryak S,Bandwar RP,Santockyte R

更新日期：2019-09-01 00:00:00

abstract：:Rabies is an infectious viral disease, characterized as a neglected zoonosis, responsible for nearly 60,000 deaths annually. The virus is transmitted mainly by dogs in Africa and Asia, and wildlife in Europe and the Americas, to all mammals' species, causing severe encephalitis almost always fatal after the onset of n...

journal_title：Journal of virological methods

authors： Lourenç Correia Moreira B,Aparecida Pereira L,Lappas Gimenez AP,Minor Fernandes Inagaki J,Raboni SM

更新日期：2019-08-01 00:00:00

abstract：:Acute bee paralysis virus (ABPV), Black queen cell virus (BQCV), Chronic bee paralysis virus (CBPV), Deformed wing virus (DWV), Sacbrood virus (SBV) and Varroa destructor virus 1 (VDV1) are the six main honeybee viruses reported in Europe. We assessed the accuracy (trueness and precision) of reverse transcriptase quan...

journal_title：Journal of virological methods

authors： Schurr F,Tison A,Militano L,Cheviron N,Sircoulomb F,Rivière MP,Ribière-Chabert M,Thiéry R,Dubois E

更新日期：2019-08-01 00:00:00

abstract：:Fowl adenovirus serotype-4 (FAdV-4) has been recognized as a predominant threat to the broilers aged from three to five weeks. Hydropericardium syndrome (HPS) is one of its major clinical diseases by FAdV-4 resulting in heavy economic losses. In this study, a loop-mediated isothermal amplification coupling with a late...

journal_title：Journal of virological methods

authors： Zhai X,Mei X,Wu X,Zuo L,Zhou L,Tian Y,Han X,Yang X,Wang H

更新日期：2019-08-01 00:00:00

abstract：:Citrus leaf blotch virus (CLBV) is a type member of the genus Citrivirus belonging to Betaflexiviridae. In this study, a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method was developed to detect CLBV; this technology has been widely used in the detection of various plant pathogenic microorg...

journal_title：Journal of virological methods

authors： Liu H,Wu W,Tan J,Li Y,Mi W,Jiang L,Wu Y

更新日期：2019-08-01 00:00:00

abstract：:A multiplex quantitative real-time polymerase chain reaction (mqPCR) assay was developed and validated for the detection and differentiation of porcine circovirus type 3 (PCV3) and type 2 (PCV2) strains. The assay coverage was 97.9% (184/188) for PCV3 and 99.1% (1889/1907) for PCV2 sequences that were available from t...

journal_title：Journal of virological methods

authors： Wang Y,Feng Y,Zheng W,Noll L,Porter E,Potter M,Cino G,Peddireddi L,Liu X,Anderson G,Bai J

更新日期：2019-07-01 00:00:00

abstract：:Mungbean yellow mosaic India virus (MYMIV) infecting soybean and other legumes causes yellow mosaic disease (YMD). Evaluation of soybean genotypes for YMD resistance involves field screening at disease hot spots or in a protected environment using infectious clones or viruliferous whiteflies as sources of virus inocul...

journal_title：Journal of virological methods

authors： Ramesh SV,Shivakumar M,Ramteke R,Bhatia VS,Chouhan BS,Goyal S,Singh A,Praveen S,Gill BS,Chand S

更新日期：2019-06-01 00:00:00

abstract：:In recent years, there has been an increase in reported cases of fowl adenovirus serotype 4 (FAdV-4) in chickens in China. The use of live attenuated vaccines contaminated with FAdV-4 has been proved to be one of the important causes of massive outbreaks of hydropericardium syndrome. To detect the contamination with F...

journal_title：Journal of virological methods

authors： Dong G,Meng F,Zhang Y,Cui Z,Lidan H,Chang S,Zhao P

更新日期：2019-03-01 00:00:00

abstract：:Hepatitis C Virus c33, a recombinant protein comprising residues 1192-1457 of NS3 helicase, has been a mainstay of HCV serology for decades. With seven unpaired cysteines, seroreactivity of E. coli expressed c33 is dependant on reductants. While engineering a c33 replacement for new anti-HCV serological tests, we soug...

journal_title：Journal of virological methods

authors： Marohnic CC,Birkenmeyer LG,Bogdan MF,Frias EC,Otis KS,Palafox MAP,McSherry TD,Gregory SD,Zhao C,Gutierrez RA,Prostko JC,Muerhoff AS

更新日期：2019-02-01 00:00:00

abstract：:Molecular assays are currently the most widely used method to quantify pathogenic viruses in water; however, their performance is often disrupted by matrices present in environmental samples. The present study used ferrihydrite (Fh) treatment to mitigate inhibition of RT-qPCR virus detection from environmental water c...

journal_title：Journal of virological methods

authors： Canh VD,Osawa H,Inoue K,Kasuga I,Takizawa S,Furumai H,Katayama H

更新日期：2019-01-01 00:00:00

abstract：:Spring viremia of carp, a fatal viral disease, is caused by the spring viremia of carp virus (SVCV) and can result in up to 70% mortalities in common carps and significant economic losses in several other cyprinid aquaculture. The present study aimed to investigate the possible control of SVCV replication in Epithelio...

journal_title：Journal of virological methods

authors： Fouad AM,Soliman H,Abdallah ESH,Ibrahim S,El-Matbouli M,Elkamel AA

更新日期：2019-01-01 00:00:00

abstract：:HIV-1 infection cannot be cured due to the presence of the latent reservoir (LR). Novel cure or treatment strategies, such as "shock and kill" or therapeutic vaccination, aim to reduce or eradicate the LR. Cure strategies utilise robust DNA quantification assays to measure the change in the LR in low copy scenarios. N...

journal_title：Journal of virological methods

authors： Thomas J,Ruggiero A,Procopio FA,Pantaleo G,Paxton WA,Pollakis G

更新日期：2019-01-01 00:00:00

abstract：:Coliphages are alternative fecal indicators that may be suitable surrogates for viral pathogens, but majority of standard detection methods utilize insufficient volumes for routine detection in environmental waters. We compared three somatic and F+ coliphage methods based on a paired measurement from 1 L samples colle...

journal_title：Journal of virological methods

authors： McMinn BR,Rhodes ER,Huff EM,Wanjugi P,Ware MM,Nappier SP,Cyterski M,Shanks OC,Oshima K,Korajkic A

更新日期：2018-11-01 00:00:00