听力与言语-语言病理学

行为科学

医学伦理学

你正在浏览JOURNAL OF VIROLOGICAL METHODS期刊下所有文献
  • A new assay for quantitative detection of hepatitis A virus.

    abstract::Hepatitis A virus (HAV) is mainly transmitted via contaminated food or water or through person-to-person contact. Here, we describe development and evaluation of a reverse transcription droplet digital PCR (RT-ddPCR) and reverse transcription real-time PCR (RT-qPCR) assay for detection of HAV in food and clinical spec...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2020.114010

    authors: Persson S,Alm E,Karlsson M,Enkirch T,Norder H,Eriksson R,Simonsson M,Ellström P

    更新日期:2021-02-01 00:00:00

  • Evaluating SARS-CoV-2 spike and nucleocapsid proteins as targets for antibody detection in severe and mild COVID-19 cases using a Luminex bead-based assay.

    abstract::Large-scale serosurveillance of severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) will only be possible if serological tests are sufficiently reliable, rapid and affordable. Many assays are either labour-intensive and require specialised facilities (e.g. virus neutralization assays), or are expensive w...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2020.114025

    authors: Mariën J,Ceulemans A,Michiels J,Heyndrickx L,Kerkhof K,Foque N,Widdowson MA,Mortgat L,Duysburgh E,Desombere I,Jansens H,Van Esbroeck M,Ariën KK

    更新日期:2021-02-01 00:00:00

  • Immunoblots may not be effective in confirming the recency of HIV-1 infection.

    abstract::Recently, immunoblots (IBs) have tended to substitute Western blots (WBs) for HIV infection diagnosis. Several studies have confirmed IBs' high sensitivity to confirm HIV infection for every stage. Since the nature and pattern of the antigens of IBs are different from those of WB, the abilities of IBs and WBs to disti...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2021.114074

    authors: Mariaggi AA,Gardiennet E,Stefic K,Essat A,Cheret A,Goujard C,Meyer L,Barin F,Avettand-Fenoel V,ANRS PRIMO cohort.

    更新日期:2021-01-21 00:00:00

  • Duplex SYBR Green I-based real-time PCR assay for the rapid detection of canine kobuvirus and canine astrovirus.

    abstract::A duplex SYBR Green I-based real-time PCR assay was established for the simultaneous detection of canine kobuvirus (CaKoV) and canine astrovirus (CaAstV). This assay can easily distinguish the two viruses according to their different melting temperatures (Tm) of 80 °C for CaKoV and 86.5 °C for CaAstV; other canine ent...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2021.114066

    authors: Wang Y,Li Y,Cui Y,Jiang S,Liu H,Wang J,Li Y

    更新日期:2021-01-13 00:00:00

  • An in-well direct lysis method for rapid detection of SARS-CoV-2 by real time RT-PCR in eSwab specimens.

    abstract:BACKGROUND:Diagnostic real time reverse transcription PCR (rRT-PCR) is usually done using nucleic acid (NA) purified from the sample. In the SARS-CoV-2 pandemic reagents and utensils for NA purification has been in short supply. This has generated interest in methods that eliminate the need for NA purification. OBJECT...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2021.114062

    authors: Jørgensen RL,Pedersen MS,Chauhan AS,Andreasson LM,Kristiansen GQ,Lisby JG,Rosenstierne MW,Schønning K

    更新日期:2021-01-08 00:00:00

  • Evaluation of SYBR Green real time PCR for detecting SARS-CoV-2 from clinical samples.

    abstract::The pandemic caused by SARS-CoV-2 has triggered an extraordinary collapse of healthcare systems and hundred thousand of deaths worldwide. Following the declaration of the outbreak as a Public Health Emergency of International Concern by the World Health Organization (WHO) on January 30th, 2020, it has become imperativ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2020.114035

    authors: Pereira-Gómez M,Fajardo Á,Echeverría N,López-Tort F,Perbolianachis P,Costábile A,Aldunate F,Moreno P,Moratorio G

    更新日期:2020-12-04 00:00:00

  • Long amplicon sequencing for improved genetic characterization of African swine fever virus.

    abstract::African Swine Fever Virus (ASFV) causes a transmissible and fatal disease in pigs that is currently devastating global swine production. Efficient and economical collection of genetic data from ASFV field isolates is essential for bio-surveillance, to limit and control its spread, and to better understand ASF disease ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2020.113946

    authors: Meekins DA,Trujillo JD,Gaudreault NN,Morozov I,Pérez-Núñez D,Revilla Y,Richt JA

    更新日期:2020-11-01 00:00:00

  • Screening of PRRSV- and ASFV-encoded proteins involved in the inflammatory response using a porcine iGLuc reporter.

    abstract::Inflammasome plays a major role in innate immune responses by activating caspase-1, resulting in secretion of interleukin-1β (IL-1β) and inflammatory pathologic responses. IL-1β release is widely used as an indirect readout to study inflammasome activation. Here we report an iGLuc reporter (pro-IL-1β-Gluc) of pig orig...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2020.113958

    authors: Song J,Li K,Li T,Zhao G,Zhou S,Li H,Li J,Weng C

    更新日期:2020-11-01 00:00:00

  • Madin-Darby bovine kidney (MDBK) cells are a suitable cell line for the propagation and study of the bovine poxvirus lumpy skin disease virus.

    abstract::Lumpy skin disease virus (LSDV) is a poxvirus that causes systemic disease in cattle, resulting in substantial economic loss to affected communities. LSDV is a rapidly emerging pathogen of growing global concern that recently spread from Africa and the Middle East into Europe and Asia, impacting the cattle population ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2020.113943

    authors: Fay PC,Cook CG,Wijesiriwardana N,Tore G,Comtet L,Carpentier A,Shih B,Freimanis G,Haga IR,Beard PM

    更新日期:2020-11-01 00:00:00

  • Evaluation of three different filters and two methods for recovering viruses from drinking water.

    abstract::Among the enteric viruses implicated in waterborne outbreaks, human norovirus and hepatitis A virus (HAV) are a serious public health issue. Most foodborne viruses are difficult or currently unlikely to cultivate. Because of the lack of a cell culture method, real-time reverse transcriptase PCR is commonly used for th...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2020.113939

    authors: Hennechart-Collette C,Dehan O,Fraisse A,Martin-Latil S,Perelle S

    更新日期:2020-10-01 00:00:00

  • Brief validation of the novel GeneXpert Xpress SARS-CoV-2 PCR assay.

    abstract::The clinical and epidemiologic management of the SARS-CoV-2 pandemic is critically dependent on molecular assays with short turn-around time. We validated the novel Xpert Xpress SARS-CoV-2 assay using a commercial nucleic acid testing (Roche Cobas 6800). We found an excellent concordance over a range of SARS-CoV-2 loa...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2020.113925

    authors: Goldenberger D,Leuzinger K,Sogaard KK,Gosert R,Roloff T,Naegele K,Cuénod A,Mari A,Seth-Smith H,Rentsch K,Hinić V,Hirsch HH,Egli A

    更新日期:2020-10-01 00:00:00

  • Colonisation of mice and pigs by a chimeric porcine circovirus 1-2 prototype vaccine strain and a PCV2 isolate originating in China and their induction of cytokines.

    abstract::A chimeric porcine circovirus (PCV) 1-2b vaccine strain and its parental wild-type PCV2b strain from China (PCV2-J) were used separately to vaccinate BALB/c mice and tissue and serum samples were collected from the mice to investigate whether the replication properties of the viruses differed. The spleen lymphocytes f...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2020.113905

    authors: Cui X,Wang X,Gao Q,Liu X,Kai Y,Chen C,Gao S

    更新日期:2020-09-01 00:00:00

  • Detection of African swine fever virus in cell culture and wild boar tissues using a commercially available monoclonal antibody.

    abstract::Following the introduction of African swine fever virus (ASFV) into Europe in 2007, ASFV infection has spread continuously over the past years and it became a high level disease threat in Europe and also Asia. Examination of suspect clinical cases for ASF with rapid and sensitive laboratory methods can substantially c...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2020.113886

    authors: Szeredi L,Bakcsa E,Zádori Z,Mészáros I,Olasz F,Bálint Á,Locsmándi G,Erdélyi K

    更新日期:2020-05-23 00:00:00

  • Virus isolation and full-length genome sequencing of a representative canine distemper virus wild type strain of the South America 2 clade.

    abstract::Canine Distemper Virus (CDV) is a highly contagious pathogen of dogs that causes severe respiratory, gastrointestinal and nervous signs. Although vaccines have been used to prevent infections, CDV has been reported worldwide, even in vaccinated animals. In the present study, a representative wild type CDV strain (Arg2...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2020.113857

    authors: Romanutti C,Keller L,La Torre J,Panzera Y,Fuques E,Pérez R,Gallo Calderon M

    更新日期:2020-05-01 00:00:00

  • Evaluation of the cobas® HCV test for quantifying HCV RNA in dried plasma spots collected using the cobas® Plasma Separation Card.

    abstract:OBJECTIVE:This study evaluated the performance of the cobas® hepatitis C virus (HCV) Test for use on the cobas® 6800/8800 Systems for the detection and quantification of HCV RNA collected using the cobas® Plasma Separation Card (PSC) compared with plasma samples. METHODS:Whole EDTA-venous blood was collected from 50 H...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2020.113820

    authors: Marins EG,Krey N,Becker A,Melzer S,Hoppler M

    更新日期:2020-04-01 00:00:00

  • Development of RT-qPCR assays for the detection of three latent viruses of pome.

    abstract::Latent fruit tree viruses present economic threat to the industry and nurseries as diseases they cause not only reduce fruit quality and production yield, but can also be spread inadvertently through propagation due to the lack of viral symptoms on an infected mother plant. As a result, these viruses require appropria...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2020.113836

    authors: Beaver-Kanuya E,Harper SJ

    更新日期:2020-04-01 00:00:00

  • Analysis of preferred codon usage in the coronavirus N genes and their implications for genome evolution and vaccine design.

    abstract::The nucleocapsid (N) protein of a coronavirus plays a crucial role in virus assembly and in its RNA transcription. It is important to characterize a virus at the nucleotide level to discover the virus's genomic sequence variations and similarities relative to other viruses that could have an impact on the functions of...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2019.113806

    authors: Sheikh A,Al-Taher A,Al-Nazawi M,Al-Mubarak AI,Kandeel M

    更新日期:2020-03-01 00:00:00

  • Predicting whole genome sequencing success for archived avian influenza virus (Orthomyxoviridae) samples using real-time and droplet PCRs.

    abstract::Long-term viral archives are valuable sources of research data. Each archive can store hundreds of thousands of diverse sample types. In the current era of whole genome sequencing, archived samples become a rich source of evolutionary and epidemiological data that can span years, and even decades. However, the ability...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2019.113777

    authors: Hopken MW,Piaggio AJ,Pabilonia KL,Pierce J,Anderson T,Abdo Z

    更新日期:2020-02-01 00:00:00

  • Generation and molecular docking analysis of specific single-chain variable fragments selected by phage display against the recombinant nucleocapsid protein of fig mosaic virus.

    abstract::The mosaic disease caused by fig mosaic virus (FMV) is considered the plague of fig worldwide. A naïve phage display library, raised against the recombinant nucleocapsid protein of FMV (FMV-Np) was screened to obtain specific monoclonal recombinant antibodies in the form of single chain variable fragments (scFvs). Aft...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2019.113796

    authors: Shahmirzaie M,Safarnejad MR,Rakhshandehroo F,Safarpour H,Shirazi FH,Zamanizadeh HR,Elbeaino T

    更新日期:2020-02-01 00:00:00

  • Methods for successful inactivation of Rift Valley fever virus in infected mosquitoes.

    abstract::Ensuring the successful inactivation of select agent material is critical for maintaining compliance with federal regulations and safeguarding laboratory personnel from exposure to dangerous pathogens. Rift Valley fever virus (RVFV), naturally transmitted by mosquitoes, is classified as a select agent by the CDC and U...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2019.113794

    authors: Bergren NA,Patterson EI,Blair H,Ellis RP,Kading RC

    更新日期:2020-02-01 00:00:00

  • A rapid RT-LAMP assay for the detection of all four lineages of Peste des Petits Ruminants Virus.

    abstract::Peste des petits ruminants (PPR) is a viral disease of small ruminants that is caused by the PPR virus (PPRV) and is a significant burden on subsistence farmers across the developing world. Loop-mediated isothermal amplification (LAMP) provides cost-effective, rapid, specific and sensitive detection of nucleic acid an...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2019.113730

    authors: Rajko-Nenow P,Flannery J,Arnold H,Howson ELA,Darpel K,Stedman A,Corla A,Batten C

    更新日期:2019-12-01 00:00:00

  • A portable detection assay for Apple stem pitting virus using reverse transcription-recombinase polymerase amplification.

    abstract::A molecular diagnostic assay for the rapid, sensitive and specific detection of Apple stem pitting virus (ASPV) in infected samples, utilizing reverse transcription-recombinase polymerase amplification (RT-RPA) at an isothermal constant temperature of 42 °C and the designed target-specific primers, was developed. The ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2019.113747

    authors: Kim NY,Lee HJ,Jeong RD

    更新日期:2019-12-01 00:00:00

  • Rapid detection of giant salamander iridovirus by cross-priming amplification.

    abstract::Giant salamander iridovirus (GSIV) belongs to the epizootic genus Ranavirus, and is the cause of epidemic diseases associated with high mortality and great losses to artificial breeding and farming. Here, we established a simple, accurate, and reliable cross-priming amplification (CPA) method to detect GSIV. The CPA a...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2019.113678

    authors: Zhang H,Wang F,Jia R,Fan J,Hao J,Du J,Niu Y,Han S,Deng R,Zhang G

    更新日期:2019-12-01 00:00:00

  • TaqMan real-time and conventional nested PCR tests specific to yellow head virus genotype 7 (YHV7) identified in giant tiger shrimp in Australia.

    abstract::In 2013, a unique seventh yellow head virus genotype (YHV7) was detected in Black Tiger shrimp (Penaeus monodon) broodstock that suffered high mortality following their capture from Joseph Bonaparte Gulf (JBG) in northern Australia. To assist with its diagnosis and assessment of its distribution, prevalence and pathog...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2019.113689

    authors: Cowley JA,Rao M,Mohr P,Moody NJ,Sellars MJ,Crane MS

    更新日期:2019-11-01 00:00:00

  • Analytical evaluation of the microarray-based FluChip-8G Influenza A+B Assay.

    abstract:BACKGROUND:Influenza causes a significant annual disease burden, with characterization of the infecting virus important in clinical and public health settings. Rapid immunoassays are fast but insensitive, whereas real-time RT-PCR is sensitive but susceptible to genetic mutations and often requires multiple serial assay...

    journal_title:Journal of virological methods

    pub_type: 杂志文章,多中心研究

    doi:10.1016/j.jviromet.2019.113686

    authors: Taylor AW,Dawson ED,Blair RH,Johnson JE Jr,Slinskey AH,Smolak AW,Toth E,Liikanen K,Stoughton RS,Smith C,Talbot S,Rowlen KL

    更新日期:2019-11-01 00:00:00

  • Evaluation of currently available bovine viral diarrhoea virus (BVDV) and HoBi-like pestivirus (HoBiPeV) specific diagnostic tests in detection of highly divergent HoBiPeVs in cattle.

    abstract::The emergence of novel and divergent HoBi-like pestivirus (HoBiPeV) strains in cattle in Asia recently has raised concerns with regard to their reliable and accurate diagnosis. Hence, the aim of this study was to evaluate currently available BVDV diagnostic tests and HoBiPeV-specific diagnostic tests in detection of g...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2019.113707

    authors: Moorthy D,Mishra N,Kalaiyarasu S,Jhade SK,Singh VP

    更新日期:2019-10-01 00:00:00

  • Detection and quantification of four viruses in Prunus pollen: Implications for biosecurity.

    abstract::Pollen transmitted viruses require accurate detection and identification to minimize the risk of spread through the global import and export of pollen. Therefore in this study we developed RT-qPCR assays for the detection of Cherry leaf roll virus (CLRV), Prune dwarf virus (PDV), Prunus necrotic ringspot virus (PNRSV)...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2019.113673

    authors: Beaver-Kanuya E,Harper SJ

    更新日期:2019-09-01 00:00:00

  • A flow-through cell counting assay for point-of-care enumeration of CD4 T-cells.

    abstract::CD4 T-cell count is a priority for staging HIV disease and guiding clinical management as part of HIV care. Conventional CD4 T-cell enumeration methods based on flow cytometry are expensive, require well-trained personnel, and are challenging to use in rural, resource-scarce areas. A simple CD4 T-cell count test that ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2019.05.012

    authors: Bystryak S,Bandwar RP,Santockyte R

    更新日期:2019-09-01 00:00:00

  • Development and validation of a real-time RT-PCR assay for the quantification of rabies virus as quality control of inactivated rabies vaccines.

    abstract::Rabies is an infectious viral disease, characterized as a neglected zoonosis, responsible for nearly 60,000 deaths annually. The virus is transmitted mainly by dogs in Africa and Asia, and wildlife in Europe and the Americas, to all mammals' species, causing severe encephalitis almost always fatal after the onset of n...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2019.04.025

    authors: Lourenç Correia Moreira B,Aparecida Pereira L,Lappas Gimenez AP,Minor Fernandes Inagaki J,Raboni SM

    更新日期:2019-08-01 00:00:00

  • Validation of quantitative real-time RT-PCR assays for the detection of six honeybee viruses.

    abstract::Acute bee paralysis virus (ABPV), Black queen cell virus (BQCV), Chronic bee paralysis virus (CBPV), Deformed wing virus (DWV), Sacbrood virus (SBV) and Varroa destructor virus 1 (VDV1) are the six main honeybee viruses reported in Europe. We assessed the accuracy (trueness and precision) of reverse transcriptase quan...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2019.04.020

    authors: Schurr F,Tison A,Militano L,Cheviron N,Sircoulomb F,Rivière MP,Ribière-Chabert M,Thiéry R,Dubois E

    更新日期:2019-08-01 00:00:00

  • A loop-mediated isothermal amplification coupling with a lateral flow dipstick for rapid and specific detection of fowl adenovirus serotype-4.

    abstract::Fowl adenovirus serotype-4 (FAdV-4) has been recognized as a predominant threat to the broilers aged from three to five weeks. Hydropericardium syndrome (HPS) is one of its major clinical diseases by FAdV-4 resulting in heavy economic losses. In this study, a loop-mediated isothermal amplification coupling with a late...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2019.04.026

    authors: Zhai X,Mei X,Wu X,Zuo L,Zhou L,Tian Y,Han X,Yang X,Wang H

    更新日期:2019-08-01 00:00:00

  • Development and evaluation of a one-step reverse transcription loop-mediated isothermal amplification for detection of Citrus leaf blotch virus.

    abstract::Citrus leaf blotch virus (CLBV) is a type member of the genus Citrivirus belonging to Betaflexiviridae. In this study, a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method was developed to detect CLBV; this technology has been widely used in the detection of various plant pathogenic microorg...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2019.05.009

    authors: Liu H,Wu W,Tan J,Li Y,Mi W,Jiang L,Wu Y

    更新日期:2019-08-01 00:00:00

  • A multiplex real-time PCR assay for the detection and differentiation of the newly emerged porcine circovirus type 3 and continuously evolving type 2 strains in the United States.

    abstract::A multiplex quantitative real-time polymerase chain reaction (mqPCR) assay was developed and validated for the detection and differentiation of porcine circovirus type 3 (PCV3) and type 2 (PCV2) strains. The assay coverage was 97.9% (184/188) for PCV3 and 99.1% (1889/1907) for PCV2 sequences that were available from t...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2019.03.011

    authors: Wang Y,Feng Y,Zheng W,Noll L,Porter E,Potter M,Cino G,Peddireddi L,Liu X,Anderson G,Bai J

    更新日期:2019-07-01 00:00:00

  • Quantification of a legume begomovirus to evaluate soybean genotypes for resistance to yellow mosaic disease.

    abstract::Mungbean yellow mosaic India virus (MYMIV) infecting soybean and other legumes causes yellow mosaic disease (YMD). Evaluation of soybean genotypes for YMD resistance involves field screening at disease hot spots or in a protected environment using infectious clones or viruliferous whiteflies as sources of virus inocul...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2019.03.002

    authors: Ramesh SV,Shivakumar M,Ramteke R,Bhatia VS,Chouhan BS,Goyal S,Singh A,Praveen S,Gill BS,Chand S

    更新日期:2019-06-01 00:00:00

  • Development and evaluation of a droplet digital PCR assay for the detection of fowl adenovirus serotypes 4 and 10 in attenuated vaccines.

    abstract::In recent years, there has been an increase in reported cases of fowl adenovirus serotype 4 (FAdV-4) in chickens in China. The use of live attenuated vaccines contaminated with FAdV-4 has been proved to be one of the important causes of massive outbreaks of hydropericardium syndrome. To detect the contamination with F...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2018.09.005

    authors: Dong G,Meng F,Zhang Y,Cui Z,Lidan H,Chang S,Zhao P

    更新日期:2019-03-01 00:00:00

  • Engineering of the hepatitis C virus helicase for enhanced seroreactivity.

    abstract::Hepatitis C Virus c33, a recombinant protein comprising residues 1192-1457 of NS3 helicase, has been a mainstay of HCV serology for decades. With seven unpaired cysteines, seroreactivity of E. coli expressed c33 is dependant on reductants. While engineering a c33 replacement for new anti-HCV serological tests, we soug...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2018.10.019

    authors: Marohnic CC,Birkenmeyer LG,Bogdan MF,Frias EC,Otis KS,Palafox MAP,McSherry TD,Gregory SD,Zhao C,Gutierrez RA,Prostko JC,Muerhoff AS

    更新日期:2019-02-01 00:00:00

  • Ferrihydrite treatment to mitigate inhibition of RT-qPCR virus detection from large-volume environmental water samples.

    abstract::Molecular assays are currently the most widely used method to quantify pathogenic viruses in water; however, their performance is often disrupted by matrices present in environmental samples. The present study used ferrihydrite (Fh) treatment to mitigate inhibition of RT-qPCR virus detection from environmental water c...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2018.10.018

    authors: Canh VD,Osawa H,Inoue K,Kasuga I,Takizawa S,Furumai H,Katayama H

    更新日期:2019-01-01 00:00:00

  • In-vitro inhibition of spring viremia of carp virus replication by RNA interference targeting the RNA-dependent RNA polymerase gene.

    abstract::Spring viremia of carp, a fatal viral disease, is caused by the spring viremia of carp virus (SVCV) and can result in up to 70% mortalities in common carps and significant economic losses in several other cyprinid aquaculture. The present study aimed to investigate the possible control of SVCV replication in Epithelio...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2018.10.008

    authors: Fouad AM,Soliman H,Abdallah ESH,Ibrahim S,El-Matbouli M,Elkamel AA

    更新日期:2019-01-01 00:00:00

  • Comparative analysis and generation of a robust HIV-1 DNA quantification assay.

    abstract::HIV-1 infection cannot be cured due to the presence of the latent reservoir (LR). Novel cure or treatment strategies, such as "shock and kill" or therapeutic vaccination, aim to reduce or eradicate the LR. Cure strategies utilise robust DNA quantification assays to measure the change in the LR in low copy scenarios. N...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2018.10.010

    authors: Thomas J,Ruggiero A,Procopio FA,Pantaleo G,Paxton WA,Pollakis G

    更新日期:2019-01-01 00:00:00

  • Comparison of somatic and F+ coliphage enumeration methods with large volume surface water samples.

    abstract::Coliphages are alternative fecal indicators that may be suitable surrogates for viral pathogens, but majority of standard detection methods utilize insufficient volumes for routine detection in environmental waters. We compared three somatic and F+ coliphage methods based on a paired measurement from 1 L samples colle...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2018.08.007

    authors: McMinn BR,Rhodes ER,Huff EM,Wanjugi P,Ware MM,Nappier SP,Cyterski M,Shanks OC,Oshima K,Korajkic A

    更新日期:2018-11-01 00:00:00

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