Abstract：
:Lumpy skin disease virus (LSDV) is a poxvirus that causes systemic disease in cattle, resulting in substantial economic loss to affected communities. LSDV is a rapidly emerging pathogen of growing global concern that recently spread from Africa and the Middle East into Europe and Asia, impacting the cattle population in these regions. An increase in research efforts into LSDV is required to address key knowledge gaps, however this is hampered by lack of suitable cell lines on which to propagate and study the virus. In this work we describe the replication and spread of LSDV on Madin-Darby bovine kidney (MDBK) cells, and the formation of foci-type poxvirus plaques by LSDV on MDBK cells. Methods utilising MDBK cells to quantify neutralising antibodies to LSDV, and to purify LSDV genomic DNA suitable for short read sequencing are described. These research methods broaden the tools available for LSDV researchers and will facilitate the gathering of evidence to underpin the development of LSD control and prevention programmes.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Fay PC,Cook CG,Wijesiriwardana N,Tore G,Comtet L,Carpentier A,Shih B,Freimanis G,Haga IR,Beard PMdoi
10.1016/j.jviromet.2020.113943subject
Has Abstractpub_date
2020-11-01 00:00:00pages
113943eissn
0166-0934issn
1879-0984pii
S0166-0934(20)30195-6journal_volume
285pub_type
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