Abstract：
:Epstein-Barr virus (EBV) has been implicated in the pathogenesis of several human malignancies including B lymphomas and nasopharyngeal carcinoma. The EBV R transactivator (Rta) has been found to play essential roles in stimulating a lytic cycle and viral gene expression. Recently, it was shown that ELISA detecting serum IgG-Rta(150+185) (two internal fragments of Rta) levels may be useful as a serological parameter to assist in the diagnosis of nasopharyngeal carcinoma. The present studies were to prepare monoclonal antibodies specific for the Rta185 and provide a useful tool for the detection of Rta. For this purpose, two monoclonal antibodies (Mabs) specific for the Rta185 were generated. They were identified by Western blot, enzyme-linked immunosorbent assay (ELISA) and immunofluorescence analysis. The results revealed two different immunofluorescence patterns in EBV-positive B cells and epithelial cells, and suggested that there might be a difference in EBV replication mode between B cells and epithelial cells. The Mabs obtained in this study have a potential for the diagnosis of EBV associated diseases.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Zhu YH,Wei YS,Li H,Liang WB,Du B,Zhang GQ,Zhang Ldoi
10.1016/j.jviromet.2007.03.011subject
Has Abstractpub_date
2007-09-01 00:00:00pages
12-6issue
1-2eissn
0166-0934issn
1879-0984pii
S0166-0934(07)00103-6journal_volume
144pub_type
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pub_type： 杂志文章
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