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Half-genome human immunodeficiency virus type 1 constructs for rapid production of reporter viruses.

Abstract：

:The use of reporter constructs of HIV-1 molecular clones has been useful in studying the effects of infection on individual cells. A strategy of producing viral half-genome constructs containing reporter genes that can be used to produce recombinant complete infectious virions is described. This approach allows rapid generation of reporter and non-reporter virions from the same genetic construct, obviating the need to produce a new reporter genetic construct for each viral change to be studied. We provide an example of the utility of this system for studying the effects of Nef on infected cells.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Ali A,Jamieson BD,Yang OO

doi

10.1016/s0166-0934(03)00110-1

subject

Has Abstract

pub_date

2003-06-30 00:00:00

pages

137-42

issue

2

eissn

0166-0934

issn

1879-0984

pii

S0166093403001101

journal_volume

110

pub_type

杂志文章

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