Abstract:
:Integration of reverse transcribed viral DNA of HIV into host chromosomes is mediated by the viral enzyme, integrase. This enzymatic activity can be monitored in vitro by integration of a small labeled DNA (donor) into a second unlabeled DNA (target). The methodology usually involves isotope labeling and gel electrophoresis. To simplify the measurement, a method mimicking enzyme-linked immunosorbent assay (ELISA) procedures was developed. Fragments of DNA were adsorbed directly on 96-well plates and used as the target DNA. The donor was a synthetic 21-bp DNA duplex of HIV-1 U5 LTR; biotin was incorporated into the 5' end of one strand whose two nucleotides at the 3' end were specifically removed during the integration. As a result of integration, the biotin-labeled donor DNA was joined with the target DNA and became immobilized on plates. These integration products were then measured by binding of avidin-alkaline phosphatase on plates. The method is simple and straightforward and can easily be adapted for high throughput screening of integrase inhibitors.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Chang YC,Ching TT,Syu WJdoi
10.1016/0166-0934(96)02033-2subject
Has Abstractpub_date
1996-05-01 00:00:00pages
135-40issue
1-2eissn
0166-0934issn
1879-0984pii
0166-0934(96)02033-2journal_volume
59pub_type
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/j.jviromet.2007.12.002
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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abstract::A new approach to simultaneous detection and typing of related agents by the multiplex polymerase chain reaction (PCR) is described. The reaction was been applied to human herpesviruses by nested amplification of fragments of the DNA polymerase genes. During the first amplification, primers were used as two equimolar ...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(93)90061-u
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
pub_type: 杂志文章
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/j.jviromet.2006.11.023
更新日期:2007-04-01 00:00:00
abstract::Rapid characterization of herpesvirus isolates exemplified by equine herpesviruses is described. Total DNAs were isolated from virus infected small scale cell cultures. The DNA fragments obtained after restriction enzyme digestion were separated on agarose gels, transferred and immobilized on filter membranes. A radio...
journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(86)90030-3
更新日期:1986-11-01 00:00:00
abstract::A single amino acid substitution, from histidine to tyrosine at position 274 of the neuraminidase gene has converted Oseltamivir sensitive H5N1 influenza A virus into a resistant strain. Currently, Oseltamivir is being stockpiled in many countries potentially affected by the influenza A virus subtype H5N1 epidemic. To...
journal_title:Journal of virological methods
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doi:10.1016/j.jviromet.2006.09.007
更新日期:2007-01-01 00:00:00
abstract::The sensitivity and specificity of four modes of two assays, immediate early nuclear antigen detection in cell culture (IENAD) at 24 and 48 h post-infection (p.i.) by immunofluorescence using a murine monoclonal antibody, and dot-blot DNA hybridisation with overnight or prolonged autoradiography using the 32P-labelled...
journal_title:Journal of virological methods
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journal_title:Journal of virological methods
pub_type: 杂志文章
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更新日期:2013-11-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/s0166-0934(02)00247-1
更新日期:2003-03-01 00:00:00
abstract::Ensuring the successful inactivation of select agent material is critical for maintaining compliance with federal regulations and safeguarding laboratory personnel from exposure to dangerous pathogens. Rift Valley fever virus (RVFV), naturally transmitted by mosquitoes, is classified as a select agent by the CDC and U...
journal_title:Journal of virological methods
pub_type: 杂志文章
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更新日期:2020-02-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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abstract::Congenital human cytomegalovirus infections are the major infectious cause of birth defects in the United States. How this virus crosses the placenta and causes fetal disease is poorly understood. Guinea pig cytomegalovirus (GPCMV) is a related virus that provides an important model for studying cytomegaloviral congen...
journal_title:Journal of virological methods
pub_type: 杂志文章
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abstract::A simple method for evaluating the virucidal activity of water-soluble antiseptic and disinfectant products using poliovirus type 1 (Sabin strain) is described. Using a commercial concentrator, kinetic studies of four products were carried out. It was shown that 2% glutaraldehyde and 5% povidone iodine are rapidly vir...
journal_title:Journal of virological methods
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journal_title:Journal of virological methods
pub_type: 杂志文章
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journal_title:Journal of virological methods
pub_type: 杂志文章
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journal_title:Journal of virological methods
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更新日期:2004-03-15 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/j.jviromet.2012.12.006
更新日期:2013-03-01 00:00:00
abstract::Human cytomegalovirus and human herpesvirus-6 are closely related viruses which cause similar diseases, have similar cellular repositories of latent infection, and may be detected largely in the same types of clinical specimens. DNA amplification appears likely to play an increasing role in the diagnosis of recent and...
journal_title:Journal of virological methods
pub_type: 杂志文章
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更新日期:1995-06-01 00:00:00
abstract::In recent years, there has been an increase in reported cases of fowl adenovirus serotype 4 (FAdV-4) in chickens in China. The use of live attenuated vaccines contaminated with FAdV-4 has been proved to be one of the important causes of massive outbreaks of hydropericardium syndrome. To detect the contamination with F...
journal_title:Journal of virological methods
pub_type: 杂志文章
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更新日期:2019-03-01 00:00:00