Abstract：
:The production of monoclonal antibodies (mAbs) to a common antigenic region on rabbit haemorrhagic disease virus (RHDV) has enabled the development of a capture ELISA for virus detection. The assay was shown to detect reliably the presence of viral antigen in crude homogenates of a range of rabbit tissues and has provided the first evidence for the presence of RHDV in blood, serum and heart tissue. A limited time course study of the progression of virus infection revealed viral antigen is first detected in the liver at between 18 and 24 h post-infection (p.i.) and in the spleen between 24 and 30 h p.i. The assay displayed a high level of specificity, clearly differentiating between groups of infected (lowest observed optical density (O.D.) 0.45) and uninfected (highest O.D. 0.06) rabbits. No viral antigen was detected in the urine or faeces collected from infected rabbits at post-mortem. Experiments employing the 'spiking' of pools of urine and faecal homogenates collected from uninfected rabbits, with a known amount of virus, indicated that RHDV may not be present in the faeces of infected animals and if present in urine, it appears to undergo substantial degradation.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Collins BJ,White JR,Lenghaus C,Morrissy CJ,Westbury HAdoi
10.1016/0166-0934(96)02004-6subject
Has Abstractpub_date
1996-04-26 00:00:00pages
145-54issue
1-2eissn
0166-0934issn
1879-0984pii
0166-0934(96)02004-6journal_volume
58pub_type
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