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Enzyme-linked fluorescence immunoassays using beta-galactosidase and antibodies covalently bound to polystyrene plates.

Abstract：

:A solid-phase enzyme-linked immunoassay using a fluorogenic substrate (4-methylumbelliferyl-beta-D-galactopyranoside) was developed. Antibodies were covalently linked to glutaraldehyde-activated 96-well aminopolystyrene plates. Antigens from test samples were adsorbed to the solid phase and detected using antibodies conjugated with E. coli beta-galactosidase. Glutaraldehyde, N-succinimidyl-3-(2-pyridyldithio)-propionate or N-succinimidyl-6-(4-azido-2-nitrophenylamino)-hexanoate were used as linkers between antibodies and the enzyme. The measurement of fluorescence can be automated for rapid screening of many specimens. The sensitivity limit of the test for HBsAg is about 5-10 pg.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Neurath AR,Strick N

doi

10.1016/0166-0934(81)90050-1

subject

Has Abstract

pub_date

1981-10-01 00:00:00

pages

155-65

issue

3

eissn

0166-0934

issn

1879-0984

pii

0166-0934(81)90050-1

journal_volume

3

pub_type

杂志文章

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