Non-radioactive assay of natural killer cell-mediated cytotoxicity against cytomegalovirus-infected fibroblasts by DNA fragmentation ELISA.

abstract：:Nucleic acid amplification techniques have become the mainstay for ultimate sensitivity for detecting low levels of virus, including human immunodeficiency virus (HIV). As a sophisticated technology with relative expensive reagents and instrumentation, adoption of nucleic acid testing (NAT) can be cost inhibited in se...

journal_title：Journal of virological methods

authors： Chang L,Song L,Fournier DR,Kan CW,Patel PP,Ferrell EP,Pink BA,Minnehan KA,Hanlon DW,Duffy DC,Wilson DH

更新日期：2013-03-01 00:00:00

abstract：BACKGROUND:The HIV/HCV/HBsAg Triplex consists in manually performed, visually interpreted, lateral flow, immunochromatographic rapid diagnostic test simultaneously detecting in 15min human immunodeficiency virus (HIV)-1 and HIV-2 and hepatitis C virus (HCV)- specific antibodies (Ab) (IgG and IgM) and hepatitis B virus ...

journal_title：Journal of virological methods

authors： Robin L,Mboumba Bouassa RS,Nodjikouambaye ZA,Charmant L,Matta M,Simon S,Filali M,Mboup S,Bélec L

更新日期：2018-03-01 00:00:00

abstract：:Vascular puncture inoculation (VPI) is an effective technique for transmission of maize viruses without using arthropods or other biological vectors. It involves using a jeweler's engraving tool to push minuten pins through a droplet of virus inoculum toward the major vascular bundle in the scutellum of germinating ke...

journal_title：Journal of virological methods

authors： Redinbaugh MG,Louie R,Ngwira P,Edema R,Gordon DT,Bisaro DM

更新日期：2001-11-01 00:00:00

abstract：:Infection with high-risk types of human papillomavirus (HPV) is necessary for the development of cervical cancer. However, the majority of the HPV infections are efficiently cleared by the immune system and only a minority persist and induce the development of malignant lesions. Several studies provided evidence that ...

journal_title：Journal of virological methods

authors： Gemignani F,Landi S,Chabrier A,Smet A,Zehbe I,Canzian F,Tommasino M

更新日期：2004-08-01 00:00:00

abstract：:Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed livestock which has a drastic economic impact for affected countries. Although FMDV is distributed worldwide, many regional programs have been effective eradicating this agent. In Argentina, as in many other regions of South America, the comb...

journal_title：Journal of virological methods

authors： Jaworski JP,Compaired D,Trotta M,Perez M,Trono K,Fondevila N

更新日期：2011-12-01 00:00:00

abstract：:Identification of African swine fever virus (ASFV) proteins recognised by cytotoxic T lymphocytes (CTL) from swine surviving ASFV/NH/P68 infection was assessed using expression vectors based on the Pseudomonas aeruginosa outer membrane lipoprotein I gene (oprI). Viral antigens expressed as fusion lipoproteins were sho...

journal_title：Journal of virological methods

authors： Leitão A,Malur A,Cornelis P,Martins CL

更新日期：1998-11-01 00:00:00

abstract：:A method for duplex nested RT-PCR (nRT-PCR) with internal control (IC) for the detection of Nipah virus RNA is described. Incorporation of IC RNA distinguished false and true negative results. The extrinsic RNA was added directly to the PCR master mix and co-amplified with virus specific RNA in a duplex reaction to de...

journal_title：Journal of virological methods

authors： Wacharapluesadee S,Hemachudha T

更新日期：2007-04-01 00:00:00

abstract：:A multiplex polymerase chain reaction (mPCR) was developed and evaluated for detection of pox viral infections simultaneously using clinical samples from sheep and goats. Specific primers for three pox viruses of sheep and goats including sheeppox virus (SPPV), goatpox virus (GTPV) and orf virus (ORFV) were designed t...

journal_title：Journal of virological methods

authors： Venkatesan G,Balamurugan V,Bhanuprakash V

更新日期：2014-01-01 00:00:00

abstract：:Louping ill-like virus (LI) has been recently detected in two different locations in the north of Spain and separated by only around 400 km. Using molecular approaches, the viruses causing both outbreaks have been shown to be different to LI virus, but also different to each other. They have been called SSEV (Spanish ...

journal_title：Journal of virological methods

authors： Del Cerro A,Balseiro A,Casais R,Dalton KP,Salinas L,Saiz JC,Royo LJ

更新日期：2018-05-01 00:00:00

abstract：:The small surface antigen of hepatitis B virus (HBV) was produced in Drosophila melanogaster Schneider-2 (DS-2) cells transfected stably using an inducible Drosophila metallothionein promoter. Selected clonal DS-2 cell-lines expressed and secreted large quantities of HBsAg particles consisting exclusively of non-glyco...

journal_title：Journal of virological methods

authors： Deml L,Schirmbeck R,Reimann J,Wolf H,Wagner R

更新日期：1999-05-01 00:00:00

abstract：:Formalinized chick erythrocytes agglutinated with Japanese encephalitis virus antigens over a wider range of pH (6.0-7.2) than fresh erythrocytes (pH 6.4-6.8). The virus antigens were unstable below pH 7.0 and therefore the use of the formalinized erythrocytes made it possible for a one-diluent system of the hemagglut...

journal_title：Journal of virological methods

authors： Ito H,Iwasa S

更新日期：1980-01-01 00:00:00

abstract：:Lymphocystis disease virus (LCDV) is the causative agent of lymphocystis disease affecting marine and freshwater fish worldwide. Here an antibody microarray was developed and employed to detect LCDV in fish. Rabbit anti-LCDV serum was arrayed on agarose gel-modified slides as capture antibody, and Cy3-conjugated anti-...

journal_title：Journal of virological methods

authors： Sheng X,Xu X,Zhan W

更新日期：2013-05-01 00:00:00

abstract：:A real-time RT-PCR assay based on the TaqMan technology was developed for rapid and sensitive detection of pestiviruses infecting cattle, i.e., bovine viral diarrhea virus (BVDV) 1, BVDV-2, and the emerging HoBi-like pestiviruses. The assay was linear and reproducible, being able to detect as few as 10 copies of viral...

journal_title：Journal of virological methods

authors： Losurdo M,Mari V,Lucente MS,Colaianni ML,Padalino I,Cavaliere N,Buonavoglia C,Decaro N

更新日期：2015-11-01 00:00:00

abstract：:A simple one-step ELISA to detect anti-CMV antibodies was developed. The test was based on the inhibition principle, and used anti-CMV coated microtitre plates, CMV nuclear antigen and anti-CMV Fab'-HRP conjugate; total assay time was 1.5 h. The use of Fab'-HRP conjugates improved discrimination between positive and n...

journal_title：Journal of virological methods

authors： Wielaard F,Scherders J,Dagelinckx C,Gausset P

更新日期：1987-08-01 00:00:00

abstract：:The Cucumber mosaic virus (CMV) is a three-component isodiametric plant virus with an extremely wide host range, present worldwide. A pseudorecombinant form has been described, deriving from the RNA3 component of the CMV-S strain, carrying the coat protein (CP) gene, and the RNA 1, 2 components of the CMV-D strain. Th...

journal_title：Journal of virological methods

authors： Nuzzaci M,Bochicchio I,De Stradis A,Vitti A,Natilla A,Piazzolla P,Tamburro AM

更新日期：2009-02-01 00:00:00

abstract：:Conditions for using slot-blot nucleic acid hybridization to quantitatively detect dengue-2 virus using a radiolabelled cDNA probe, pVV17, were determined. As little as 11 plaque-forming units of virus were detected using a hybridization mixture without formamide and performing the test at 70 degrees C. While predomin...

journal_title：Journal of virological methods

authors： Henchal EA,Narupiti S,Feighny R,Padmanabhan R,Vakharia V

更新日期：1987-02-01 00:00:00

abstract：:Highly pathogenic avian influenza virus H5N1 is a continuous threat to public health and poultry industry. The recurrence of the H5N1 led us to develop a robust, specific, and rapid detection method for the virus. In this study, an impedance aptasensor was developed for the virus detection using specific H5N1 aptamer ...

journal_title：Journal of virological methods

authors： Karash S,Wang R,Kelso L,Lu H,Huang TJ,Li Y

更新日期：2016-10-01 00:00:00

abstract：:A polymerase chain reaction (PCR) based on the use of multiple primers enabling the simultaneous detection of HPV-6b, -11, -16 and -18 in a single tube reaction was developed and validated on cervico-vaginal specimens, including tissues embedded in paraffin. This PCR setting proved to be specific and sensitive, allowi...

journal_title：Journal of virological methods

authors： Anceschi MM,Falcinelli C,Pieretti M,Cosmi EV

更新日期：1990-04-01 00:00:00

abstract：:Feline calicivirus (FCV) has been used by researchers as a surrogate for Norwalk virus (NV), since they share a similar genomic organization, physicochemical characteristics, and are grouped in the same family, Caliciviridae. Unlike NV, however, FCV can grow in established cell lines and produce a syncytial form of cy...

journal_title：Journal of virological methods

authors： Bidawid S,Malik N,Adegbunrin O,Sattar SA,Farber JM

更新日期：2003-02-01 00:00:00

abstract：:In order to identify new potential antiviral drugs, small amounts of extracts or compounds have to be examined for cytotoxicity and antiviral activity in primary screening using a rapid, easy, inexpensive, and highly standardised test system. In this study, high-throughput cytopathic effect (CPE) inhibitory assays wer...

journal_title：Journal of virological methods

authors： Schmidtke M,Schnittler U,Jahn B,Dahse H,Stelzner A

更新日期：2001-06-01 00:00:00

abstract：:Three anti-H5 influenza virus monoclonal antibody (mAb) clones, IFH5-26, IFH5-115 and IFH5-136, were obtained by immunising a BALB/C mouse with inactivated A/duck/Hokkaido/Vac-1/04 (H5N1). These mAbs were found to recognise specifically the haemagglutinin (HA) epitope of the influenza H5 subtypes by western blotting w...

journal_title：Journal of virological methods

authors： Miyagawa E,Kogaki H,Uchida Y,Fujii N,Shirakawa T,Sakoda Y,Kida H

更新日期：2011-05-01 00:00:00

abstract：:Herpes simplex virus type 1 (HSV-1) amplicon preparations are usually quantified as transducing units/ml (TU/ml), with little information on genomic copy/TU ratios. In the present study, two HSV-1 amplicons expressing enhanced green fluorescent protein (EGFP) were analysed by quantitative PCR (qPCR) and transducing ac...

journal_title：Journal of virological methods

authors： El-Sherbini YM,Stevenson MM,Seymour LW,Wade-Martins R

更新日期：2009-08-01 00:00:00

abstract：:An efficient procedure for the detection of avian reovirus (ARV)-specific RNA sequences in veterinary immunological medicinal products using reverse transcriptase polymerase chain reaction (RT-PCR) is described. Four ARV vaccine strains (1133, 1733, 2408 and Olson WVU2937), two ATCC strains (VR826 and VR856) as well a...

journal_title：Journal of virological methods

authors： Bruhn S,Bruckner L,Ottiger HP

更新日期：2005-02-01 00:00:00

abstract：:A direct ELISA was developed for determination of IgM antibody to varicella zoster virus (VZV). With this sensitive method VZV IgM antibodies were detected in all patients with a varicella and in 84% with a herpes zoster infection. All but one of 28 renal allograft recipients had previously had varicella. A primary in...

journal_title：Journal of virological methods

authors： Sundqvist VA

更新日期：1982-11-01 00:00:00

abstract：:A simple immunoperoxidase assay (IPA), adapted for detection of serum IgM antibodies to cytomegalovirus (CMV) is described. The antigen consisted of CMV infected human embryonic fibroblasts or isolated nuclei. The sera were absorbed with aggregated gamma-globulins prior to testing. Rabbit anti-human IgM peroxidase con...

journal_title：Journal of virological methods

authors： Singer Y,Kimmel N,Sarov I

更新日期：1985-05-01 00:00:00

abstract：:Noroviruses (NoVs) are recognized as the most common agents of outbreaks of food-borne viral gastroenteritis and the efficiency of different methods for detection of NoVs from food matrices have been tested in several laboratories worldwide. The aim of this study was to develop a rapid and sensitive method for recover...

journal_title：Journal of virological methods

authors： Fumian TM,Leite JP,Marin VA,Miagostovich MP

更新日期：2009-01-01 00:00:00

abstract：:Rice tungro disease (RTD) is a recurring disease affecting rice farming especially in the South and Southeast Asia. The disease is commonly diagnosed by visual observation of the symptoms on diseased plants in paddy fields and by polymerase chain reaction (PCR). However, visual observation is unreliable and PCR can be...

journal_title：Journal of virological methods

authors： Yee SF,Chu CH,Poili E,Sum MSH

更新日期：2017-02-01 00:00:00

abstract：:Cutting edge immune monitoring techniques increasingly measure multiple functional outputs for various cell types, such as intracellular cytokine staining (ICS) assays that measure cytokines expressed by T cells. To date, however, there is no precise method to measure virus-specific cytokine production by both T cells...

journal_title：Journal of virological methods

authors： Pattacini L,Murnane PM,Fluharty TR,Katabira E,De Rosa SC,Baeten JM,Lund JM

更新日期：2014-04-01 00:00:00

abstract：:A technique for coupling foot-and-mouth disease virus (FMDV) to tanned sheep red blood cells (SRBC) is reported. Different parameters influencing the procedure were studied. Subtypes C2, C3, O1 and A24 were used as antigens, and guinea pig hyperimmune sera obtained were tested for specific antibody in passive hemagglu...

journal_title：Journal of virological methods

authors： Pujol CA,Feledi CA,Massouh EJ

更新日期：1988-08-01 00:00:00

abstract：:Human cytomegalovirus (HCMV) genome manipulation has always been difficult. Recently, the introduction of full-length HCMV DNA into Escherichia coli as an artificial bacterial chromosome (BAC) clone has allowed reliable targeted mutagenesis. Here, we show the next generation of improvement in designing recombinant HCM...

journal_title：Journal of virological methods

authors： Wang W,Patterson CE,Yang S,Zhu H

更新日期：2004-11-01 00:00:00