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Generation of a DNA microarray for determination of E6 natural variants of human papillomavirus type 16.

Abstract：

:Infection with high-risk types of human papillomavirus (HPV) is necessary for the development of cervical cancer. However, the majority of the HPV infections are efficiently cleared by the immune system and only a minority persist and induce the development of malignant lesions. Several studies provided evidence that intratype genetic variations are implicated in determining the clinical outcome of HPV infections. In this study, we describe a DNA chip based on arrayed primer extension (APEX) for the analysis of the natural variants of HPV16, the most frequently detected type in cervical cancer world-wide. We show that HPV16 E6 variants are detected efficiently by APEX. In addition, APEX is a valid assay for the simultaneous detection of different HPV16 variants. Thus, it could be used for clinical studies aiming to analyse multiple infections with different HPV16 subtypes. Finally, since APEX combines the advantages of dideoxy DNA sequencing with the high-throughput potential of the microarray, it is suitable for the simultaneous analysis of a number of variable regions of HPV16 genome in large-scale studies.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Gemignani F,Landi S,Chabrier A,Smet A,Zehbe I,Canzian F,Tommasino M

doi

10.1016/j.jviromet.2004.02.018

subject

Has Abstract

pub_date

2004-08-01 00:00:00

pages

95-102

issue

2

eissn

0166-0934

issn

1879-0984

pii

S0166093404000692

journal_volume

119

pub_type

杂志文章

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