Abstract：
:A system which allows the reconstitution of measles virus (MV) from cloned cDNA is described. The severely host cell restricted vaccinia vector MVA-T7 expressing bacteriophage T7 RNA polymerase was used to generate full-length antigenomic MV RNA and simultaneously the mRNAs encoding the viral N, P and L proteins in order to produce replicationally and transcriptionally active nucleocapsids. The functionality of the N, P and L proteins was demonstrated first by their ability to rescue MV specific subgenomic RNAs. Assembly and budding of reconstituted MV was shown by syncytia formation and subsequently by virus isolation. The inability of MVA-T7 to produce progeny virus in most mammalian cells circumvents the necessity to separate the reconstituted MV from the MVA-T7 helper virus. Since all components are expressed transiently, this system is especially suitable for studying the functions of N, P and L. Furthermore, it is useful for investigating later steps in the MV life cycle.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Schneider H,Spielhofer P,Kaelin K,Dötsch C,Radecke F,Sutter G,Billeter MAdoi
10.1016/s0166-0934(96)02137-4subject
Has Abstractpub_date
1997-02-01 00:00:00pages
57-64issue
1eissn
0166-0934issn
1879-0984pii
S0166093496021374journal_volume
64pub_type
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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