Abstract：
:The liquid-phase blocking ELISA (LPBE) and a specific isotype assay (SIA) modified for caprine/ovine IgG1 and IgG2 were used to detect antibodies against foot-and-mouth disease virus isolate O(1) Manisa in sheep milk samples. The majority of samples from animals vaccinated 14-23 weeks previously were indistinguishable from naive sheep when tested in the LPBE but 97% were positive using the SIA. All milk samples taken at 7 days after parturition from immunised animals were positive by LPBE. Thereafter, this proportion declined, although occasional reactors were detected at every stage up to 83 days. However, when mean titres were calculated for every sampling point after lambing, results were only positive for the first 18 days in this test. By contrast, the SIA was able to detect almost all (i.e. 97%) the vaccinated animals up to 83 days post parturition. Milk samples from convalescent animals all gave positive results in the assays. Sicilo-Sarde sheep gave higher titres on average than the Commisane breed at comparable stages after lambing. Both tests could be used for surveillance purposes following an outbreak of FMD to identify flocks of sheep which had been infected, but the SIA would be required to show the presence of vaccinated animals unless they were sampled soon after parturition.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Armstrong RMdoi
10.1016/s0166-0934(97)00144-4subject
Has Abstractpub_date
1997-12-01 00:00:00pages
45-51issue
1-2eissn
0166-0934issn
1879-0984pii
S0166-0934(97)00144-4journal_volume
69pub_type
杂志文章abstract：:The fully automated IMx immunoassay analyzer was used to develop a system for the detection of IgG and IgM antibodies to rubella virus for immune status screening and diagnosis of primary infections. Reagents and assay protocol software were developed using rubella virus sensitized microparticles as the solid phase to...
journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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更新日期：1995-11-01 00:00:00
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更新日期：2008-03-01 00:00:00
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journal_title：Journal of virological methods
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更新日期：1987-05-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 临床试验,杂志文章
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更新日期：2006-09-01 00:00:00
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更新日期：2011-11-01 00:00:00
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journal_title：Journal of virological methods
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更新日期：2012-03-01 00:00:00
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更新日期：2017-01-01 00:00:00
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journal_title：Journal of virological methods
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更新日期：2011-05-01 00:00:00
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journal_title：Journal of virological methods
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更新日期：2015-04-01 00:00:00
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更新日期：1991-12-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
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journal_title：Journal of virological methods
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更新日期：2008-11-01 00:00:00
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更新日期：1984-12-01 00:00:00
