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A solid phase reverse transcriptase micro-assay for the detection of human immunodeficiency virus and other retroviruses in cell culture supernatants.

Abstract：

:A simple and rapid solid-phase reverse transcriptase assay was developed based on the use of poly(rA):oligo(dT)12-18 as template primer immobilized on DEAE cellulose paper squares to detect human immunodeficiency virus (HIV) and/or other retroviruses in cell culture supernatants. It was found that PEG (per se) -up to 4% concentrations (w/v)--did not inhibit reverse transcriptase activity. Optimal conditions of the assay were determined. This solid-phase technique is much faster and more convenient than the methods described previously.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Somogyi PA,Gyuris A,Földes I

doi

10.1016/0166-0934(90)90095-w

subject

Has Abstract

pub_date

1990-03-01 00:00:00

pages

269-76

issue

3

eissn

0166-0934

issn

1879-0984

pii

0166-0934(90)90095-W

journal_volume

27

pub_type

杂志文章

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